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The map label for this gene is spsK [H]

Identifier: 78223675

GI number: 78223675

Start: 2811352

End: 2812194

Strand: Reverse

Name: spsK [H]

Synonym: Gmet_2472

Alternate gene names: 78223675

Gene position: 2812194-2811352 (Counterclockwise)

Preceding gene: 78223676

Following gene: 78223674

Centisome position: 70.35

GC content: 64.06

Gene sequence:

>843_bases
ATGATTCTCGTCGTCGGCGCCAAGGGGATGCTCGGCCAGGACCTCATGCGGGTGCTGCCGGGAGACGTGCGCGGGGTGGA
CATCGAGGAGATCGACATCACCTCGCCTGAGTCGGTGCGTCGAGTGCTCCTGACCCTCAAGCCCCGGGTGGTGGTGAACT
GCGCCGCCTACACCGATGTCGACGGGTGCGAAACCAACGTCGACCTCGCCATGCGCGTGAACGGGGACGGTGTGGGGCAC
CTGGCAGCCGCGACCCGGGAAATCGGAGCGCTCCTCGTTCAGGTGAGCACTGATTACGTCTTCGACGGCACCAAGGGAAC
ACCCTACGTTGAGGATGATCGGGTCAATCCCCTGAGCGTCTACGGCAAATCGAAGCTCGTGGGGGAGAAAAAGGCCCGGG
AGAACCCCGACCACCTCATCGTCCGGACCCAGTGGCTCTATGGCCATGGGGGAAAGAACTTCGTGGAGACCATGCTGCGG
CTTGCCGGGGAGCGGACCGAGATCGCCGTGGTGGATGACCAGATCGGCTCCCCCACCTGGACCGCGGACCTCTCCCTCGC
CATCACCGAACTCATCGAGAACAACTGTCGGGGCACCTACCACGCCGCCAACCGGGGCACCTGCTCCTGGTACGAATTCG
CCAGGGCGATCTTCGCCGAAGCTGGCGTCGGGATGACGGTGCGCCCCCAGAGCACGGAAGAGCTGGGACGGCCGGCGCCC
CGTCCTCTCTATTCGGTCCTCGACTGCGACAAGCTTACCCATGATGCGGGGCTTGAGTTGGAGGACTGGCGCGAGGCCCT
TAAAAACTATCTGGAAAGGCGTCAGCCGGCAGTAAGAAGCTGA

Upstream 100 bases:

>100_bases
CCGAGACCGTTGACTGGTACCTGGCCAATGGCGCTTGGGTCGAGGAGGTCACCTCGGGGGCCTATCGGGACTATTACGAG
AAACAGTATGGAGGAGAGTC

Downstream 100 bases:

>100_bases
CGGGAGGGAGACCCTGTGTTGGAACGAGGAGAACGCCCCTGGGGGACCTACACGGTCCTTGAGGAAAACACCAGTTACAA
GATCAAGCGGATCGAAGTGA

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MILVVGAKGMLGQDLMRVLPGDVRGVDIEEIDITSPESVRRVLLTLKPRVVVNCAAYTDVDGCETNVDLAMRVNGDGVGH
LAAATREIGALLVQVSTDYVFDGTKGTPYVEDDRVNPLSVYGKSKLVGEKKARENPDHLIVRTQWLYGHGGKNFVETMLR
LAGERTEIAVVDDQIGSPTWTADLSLAITELIENNCRGTYHAANRGTCSWYEFARAIFAEAGVGMTVRPQSTEELGRPAP
RPLYSVLDCDKLTHDAGLELEDWREALKNYLERRQPAVRS

Sequences:

>Translated_280_residues
MILVVGAKGMLGQDLMRVLPGDVRGVDIEEIDITSPESVRRVLLTLKPRVVVNCAAYTDVDGCETNVDLAMRVNGDGVGH
LAAATREIGALLVQVSTDYVFDGTKGTPYVEDDRVNPLSVYGKSKLVGEKKARENPDHLIVRTQWLYGHGGKNFVETMLR
LAGERTEIAVVDDQIGSPTWTADLSLAITELIENNCRGTYHAANRGTCSWYEFARAIFAEAGVGMTVRPQSTEELGRPAP
RPLYSVLDCDKLTHDAGLELEDWREALKNYLERRQPAVRS
>Mature_280_residues
MILVVGAKGMLGQDLMRVLPGDVRGVDIEEIDITSPESVRRVLLTLKPRVVVNCAAYTDVDGCETNVDLAMRVNGDGVGH
LAAATREIGALLVQVSTDYVFDGTKGTPYVEDDRVNPLSVYGKSKLVGEKKARENPDHLIVRTQWLYGHGGKNFVETMLR
LAGERTEIAVVDDQIGSPTWTADLSLAITELIENNCRGTYHAANRGTCSWYEFARAIFAEAGVGMTVRPQSTEELGRPAP
RPLYSVLDCDKLTHDAGLELEDWREALKNYLERRQPAVRS

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=273, Percent_Identity=31.5018315018315, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI11034825, Length=273, Percent_Identity=31.5018315018315, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1788352, Length=288, Percent_Identity=36.8055555555556, Blast_Score=184, Evalue=5e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 30843; Mature: 30843

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVVGAKGMLGQDLMRVLPGDVRGVDIEEIDITSPESVRRVLLTLKPRVVVNCAAYTDV
CEEEEECCCCHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHCCCEEEEEEEEECCC
DGCETNVDLAMRVNGDGVGHLAAATREIGALLVQVSTDYVFDGTKGTPYVEDDRVNPLSV
CCCCCCCEEEEEECCCCCHHHHHHHHHHHEEEEEEECCEEEECCCCCCCCCCCCCCCEEE
YGKSKLVGEKKARENPDHLIVRTQWLYGHGGKNFVETMLRLAGERTEIAVVDDQIGSPTW
CCCHHHCCCHHCCCCCCEEEEEEEEEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCCCE
TADLSLAITELIENNCRGTYHAANRGTCSWYEFARAIFAEAGVGMTVRPQSTEELGRPAP
EHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCEEECCCCHHHHCCCCC
RPLYSVLDCDKLTHDAGLELEDWREALKNYLERRQPAVRS
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MILVVGAKGMLGQDLMRVLPGDVRGVDIEEIDITSPESVRRVLLTLKPRVVVNCAAYTDV
CEEEEECCCCHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHCCCEEEEEEEEECCC
DGCETNVDLAMRVNGDGVGHLAAATREIGALLVQVSTDYVFDGTKGTPYVEDDRVNPLSV
CCCCCCCEEEEEECCCCCHHHHHHHHHHHEEEEEEECCEEEECCCCCCCCCCCCCCCEEE
YGKSKLVGEKKARENPDHLIVRTQWLYGHGGKNFVETMLRLAGERTEIAVVDDQIGSPTW
CCCHHHCCCHHCCCCCCEEEEEEEEEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCCCE
TADLSLAITELIENNCRGTYHAANRGTCSWYEFARAIFAEAGVGMTVRPQSTEELGRPAP
EHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCEEECCCCHHHHCCCCC
RPLYSVLDCDKLTHDAGLELEDWREALKNYLERRQPAVRS
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]