Click here to switch to the map view.

The map label for this gene is ptsI [H]

Identifier: 78223607

GI number: 78223607

Start: 2717497

End: 2719851

Strand: Direct

Name: ptsI [H]

Synonym: Gmet_2404

Alternate gene names: 78223607

Gene position: 2717497-2719851 (Clockwise)

Preceding gene: 78223602

Following gene: 78223616

Centisome position: 67.98

GC content: 62.63

Gene sequence:

>2355_bases
ATGGCCGAAGAACAACTGGAGACGACCGGTCTGCGGACACTGGAGGATATCAGCACCCTCATCCTCAAATCCCACGACCT
CCCCGACACCCTCGACAACATCGTCCATCTGGTTGCAAAACGGATCGGCACCGATGTCTGCTCCATCTACCTGCTGGAAG
ATGACGGCAAGACCCTCCGTCTCGCGGCCAGCAAGGGGCTTTCGAAAAGCTCCGTCGGCAAGATCACCATGCAGACCTCC
GAAGGTCTCGCCGGTCTGGTAATCGAGACCAAAGGCTTGGTCACCGCCGACAACGCGCCGCTCCACCCCCGCTACAAGTA
TTTCCCCGAAACCCGCGAAGAACGGTTCAACTCCTTCCTCGGCATCCCCCTCATGCTGCGGGAGTCCCCCATGGGGGTCA
TCGTCATCCAGACCAGGGAATCGCGCACCTTCCAGCAGGAGGAGGTGAGCACCCTCACCACCATCGCCTACCAGATCTCC
AGCATCGTCATCAACGCCAAGCTTCTGGATTCCATCCGCCTGAAGGAGGCGGAACGGGAGCGGCTCGAACGGGAACTGGA
AAAATTCAAGGCCATCGGGGCGCCGGCGGGAGCGGAACCATCCGGTGACAAGCGCAAGCGGGAAAAACGTTCGCTCCTGC
TGATGGGGACGCCGGTTTCAGCGGGTTTCTCCCGGGGCAGGGCCGTCATCATCGACCGCCGGGACGAACATGACGGGATG
GGAGTGGCGACGGTGCGGTCGCGCCCCGAGGAGCGAAGCCGATTCCAGACCGCCCTCGAAAAGGCGAAGATAGAAACCAT
CTGCATGGAAAAAAGGGTAGCCGAACTCCTTTCCAAGGAGGATGCCGCCATCTTCCACACCCACCTGATGATCCTGGAAG
ATCGTGGGTTCATCGCCAAGGTCCACGACCTGATTGAGGAGGATTACGGTGCGGTACGGGCCGTGAATGAGGTGGTGGCC
CATTACGTGAGCGCTTTCTCCCGGATGGAGGACCCTTATCTCAAGGAGCGCTCTGCCGACATGGAGGATATCCGGCGCAG
GATCATCGCCTTCCTGGACGGTTCGGGCCATGCGCGGCTCAGGCTGCGGGAGAAGCGGATCCTCGTGGCCCACGAGATAC
TTCCCTCGGACATGGCCACCCTCGACCACGAGAAGCTCCTGGGGATCGTCACCGAAAAGGGGGATCTCAACTCCCACGCC
GCCATCATGGCCAAGTCCCTCGGCATACCGGCCGTGGTGGGGGTGGAGGGAATACTCCAGAAACTGGGGCTGCGGGATGA
AGTGATCGTCGACGGCAACTCGGGACGCATCTACATCAACCCGAGCGAAAAGATAAAGGGTGAGTACGAGCGGCTGGAAA
TCGACTTCGGGGTCAGGAGGCGGGAGCTTGAGGGGTTGCGGGACCTGCCGGCGGTGACCCGGGACGGCGTGGACGTGGCG
CTCAAGGCCAATATCGGCCTCTTGAGCGACATCCGGGTGGCCCTCGCCAATGGTGCCGAGGGGGTGGGGCTCTACCGGAC
CGAGTTTCCGTACATGACCCGCTCCGCCTTTCCGACCCGCAGCGAGCTCGTCGGACTCTACCGCAAGGTGCTGGAAGGCT
TCGCTCCCCATCCGGTCACCATCCGCACCCTCGACATCGGGGGGGACAAGACGCTTCCCTACTTCCCCATGGCAGCGGAG
GACAACCCCTTCATGGGATGGCGCTCCATCCGGGTGTCTTTGGAGCGGGAGGACATCTTCCGGGTCCAGTTGGCGGGGAT
CCTCCTGGCCTCGCTCCACGGCAAGGCCAGGATAATGTTCCCCATGGTGTCGAGCATCGCCGAGATCTGGCAGATCAAAA
AGATCCTCGGGGAGGTGCGCGAGGAACTGGCCCACGAAGGGCAGACCTTCGACCCCGATATCAGCTTCGGGATCATGGTG
GAACTGCCAGCTGCCGTGCAGACCGCCCACATCCTCGTGAACGAGGTCGACTTCTTCAGCATCGGCACCAACGATCTGAT
CCAGTACACCATGGCGGCCGACCGGAACAACCCGAAGGTGCGCCGATACTATGACTCCTGCCAGCCGGCGGTCCTCCACT
CCATCAAACGGGTGGTGGACGTGGCCCTGGCGACCGGAAAGCCGGTGTCGGTATGCGGCGAGATGGCCGCCGACCCGGTC
ATGGCCATCCTCCTGGTGGGAATGGGCATCCGCGAACTGAGCCTGTCGGCCCCCTCCATCCCGGTGGTCAAGCAGGCCAT
CCGCTCCGTGGATCTCCGCACGGCGCGGGAGATGGCGGACCGAGTGCTTTCCCTCGTCAGTTCCGGCGCCGTGCGTACCT
ACCTGGCAGAGACCCAGAACGCCCTGGGGCTCTGA

Upstream 100 bases:

>100_bases
CACCGCCGCAGTCACAGAAGGATGATTTTCCCAACTCCGGACTTTCCATTGACCGATTCATGGCTTATAATTAAGTCACT
TTATCGTCAAGGGCATCTAC

Downstream 100 bases:

>100_bases
CCCCCCCAAAACCACTCTCCGAACAGCAGAAAAACGCTACGTCGCCCGCAGCGCATCCACGATATTCATCCGCGCCGCCC
GGAAGGCCGGCAGCAATCCC

Product: phosphoenolpyruvate--protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 784; Mature: 783

Protein sequence:

>784_residues
MAEEQLETTGLRTLEDISTLILKSHDLPDTLDNIVHLVAKRIGTDVCSIYLLEDDGKTLRLAASKGLSKSSVGKITMQTS
EGLAGLVIETKGLVTADNAPLHPRYKYFPETREERFNSFLGIPLMLRESPMGVIVIQTRESRTFQQEEVSTLTTIAYQIS
SIVINAKLLDSIRLKEAERERLERELEKFKAIGAPAGAEPSGDKRKREKRSLLLMGTPVSAGFSRGRAVIIDRRDEHDGM
GVATVRSRPEERSRFQTALEKAKIETICMEKRVAELLSKEDAAIFHTHLMILEDRGFIAKVHDLIEEDYGAVRAVNEVVA
HYVSAFSRMEDPYLKERSADMEDIRRRIIAFLDGSGHARLRLREKRILVAHEILPSDMATLDHEKLLGIVTEKGDLNSHA
AIMAKSLGIPAVVGVEGILQKLGLRDEVIVDGNSGRIYINPSEKIKGEYERLEIDFGVRRRELEGLRDLPAVTRDGVDVA
LKANIGLLSDIRVALANGAEGVGLYRTEFPYMTRSAFPTRSELVGLYRKVLEGFAPHPVTIRTLDIGGDKTLPYFPMAAE
DNPFMGWRSIRVSLEREDIFRVQLAGILLASLHGKARIMFPMVSSIAEIWQIKKILGEVREELAHEGQTFDPDISFGIMV
ELPAAVQTAHILVNEVDFFSIGTNDLIQYTMAADRNNPKVRRYYDSCQPAVLHSIKRVVDVALATGKPVSVCGEMAADPV
MAILLVGMGIRELSLSAPSIPVVKQAIRSVDLRTAREMADRVLSLVSSGAVRTYLAETQNALGL

Sequences:

>Translated_784_residues
MAEEQLETTGLRTLEDISTLILKSHDLPDTLDNIVHLVAKRIGTDVCSIYLLEDDGKTLRLAASKGLSKSSVGKITMQTS
EGLAGLVIETKGLVTADNAPLHPRYKYFPETREERFNSFLGIPLMLRESPMGVIVIQTRESRTFQQEEVSTLTTIAYQIS
SIVINAKLLDSIRLKEAERERLERELEKFKAIGAPAGAEPSGDKRKREKRSLLLMGTPVSAGFSRGRAVIIDRRDEHDGM
GVATVRSRPEERSRFQTALEKAKIETICMEKRVAELLSKEDAAIFHTHLMILEDRGFIAKVHDLIEEDYGAVRAVNEVVA
HYVSAFSRMEDPYLKERSADMEDIRRRIIAFLDGSGHARLRLREKRILVAHEILPSDMATLDHEKLLGIVTEKGDLNSHA
AIMAKSLGIPAVVGVEGILQKLGLRDEVIVDGNSGRIYINPSEKIKGEYERLEIDFGVRRRELEGLRDLPAVTRDGVDVA
LKANIGLLSDIRVALANGAEGVGLYRTEFPYMTRSAFPTRSELVGLYRKVLEGFAPHPVTIRTLDIGGDKTLPYFPMAAE
DNPFMGWRSIRVSLEREDIFRVQLAGILLASLHGKARIMFPMVSSIAEIWQIKKILGEVREELAHEGQTFDPDISFGIMV
ELPAAVQTAHILVNEVDFFSIGTNDLIQYTMAADRNNPKVRRYYDSCQPAVLHSIKRVVDVALATGKPVSVCGEMAADPV
MAILLVGMGIRELSLSAPSIPVVKQAIRSVDLRTAREMADRVLSLVSSGAVRTYLAETQNALGL
>Mature_783_residues
AEEQLETTGLRTLEDISTLILKSHDLPDTLDNIVHLVAKRIGTDVCSIYLLEDDGKTLRLAASKGLSKSSVGKITMQTSE
GLAGLVIETKGLVTADNAPLHPRYKYFPETREERFNSFLGIPLMLRESPMGVIVIQTRESRTFQQEEVSTLTTIAYQISS
IVINAKLLDSIRLKEAERERLERELEKFKAIGAPAGAEPSGDKRKREKRSLLLMGTPVSAGFSRGRAVIIDRRDEHDGMG
VATVRSRPEERSRFQTALEKAKIETICMEKRVAELLSKEDAAIFHTHLMILEDRGFIAKVHDLIEEDYGAVRAVNEVVAH
YVSAFSRMEDPYLKERSADMEDIRRRIIAFLDGSGHARLRLREKRILVAHEILPSDMATLDHEKLLGIVTEKGDLNSHAA
IMAKSLGIPAVVGVEGILQKLGLRDEVIVDGNSGRIYINPSEKIKGEYERLEIDFGVRRRELEGLRDLPAVTRDGVDVAL
KANIGLLSDIRVALANGAEGVGLYRTEFPYMTRSAFPTRSELVGLYRKVLEGFAPHPVTIRTLDIGGDKTLPYFPMAAED
NPFMGWRSIRVSLEREDIFRVQLAGILLASLHGKARIMFPMVSSIAEIWQIKKILGEVREELAHEGQTFDPDISFGIMVE
LPAAVQTAHILVNEVDFFSIGTNDLIQYTMAADRNNPKVRRYYDSCQPAVLHSIKRVVDVALATGKPVSVCGEMAADPVM
AILLVGMGIRELSLSAPSIPVVKQAIRSVDLRTAREMADRVLSLVSSGAVRTYLAETQNALGL

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=561, Percent_Identity=39.7504456327986, Blast_Score=404, Evalue=1e-113,
Organism=Escherichia coli, GI1789193, Length=775, Percent_Identity=33.1612903225806, Blast_Score=401, Evalue=1e-112,
Organism=Escherichia coli, GI48994992, Length=460, Percent_Identity=37.3913043478261, Blast_Score=306, Evalue=3e-84,
Organism=Escherichia coli, GI1788726, Length=501, Percent_Identity=34.5309381237525, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI226510935, Length=162, Percent_Identity=27.7777777777778, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 86990; Mature: 86859

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEEQLETTGLRTLEDISTLILKSHDLPDTLDNIVHLVAKRIGTDVCSIYLLEDDGKTLR
CCCHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEE
LAASKGLSKSSVGKITMQTSEGLAGLVIETKGLVTADNAPLHPRYKYFPETREERFNSFL
EEECCCCCCCCCCEEEEECCCCCEEEEEEECCEEEECCCCCCCCCCCCCCHHHHHHHHHC
GIPLMLRESPMGVIVIQTRESRTFQQEEVSTLTTIAYQISSIVINAKLLDSIRLKEAERE
CCCEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLERELEKFKAIGAPAGAEPSGDKRKREKRSLLLMGTPVSAGFSRGRAVIIDRRDEHDGM
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCEEEEECCCCCCCCCCCEEEEECCCCCCCC
GVATVRSRPEERSRFQTALEKAKIETICMEKRVAELLSKEDAAIFHTHLMILEDRGFIAK
CEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCHHHH
VHDLIEEDYGAVRAVNEVVAHYVSAFSRMEDPYLKERSADMEDIRRRIIAFLDGSGHARL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHCCCCCCEEE
RLREKRILVAHEILPSDMATLDHEKLLGIVTEKGDLNSHAAIMAKSLGIPAVVGVEGILQ
EEECCCEEHHHHHCCCHHHHCCHHHHHEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHH
KLGLRDEVIVDGNSGRIYINPSEKIKGEYERLEIDFGVRRRELEGLRDLPAVTRDGVDVA
HCCCCCEEEEECCCCEEEECCCHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCEE
LKANIGLLSDIRVALANGAEGVGLYRTEFPYMTRSAFPTRSELVGLYRKVLEGFAPHPVT
EECCCCCHHHHHHHHHCCCCCCCEEEECCCCHHCCCCCCHHHHHHHHHHHHHCCCCCCEE
IRTLDIGGDKTLPYFPMAAEDNPFMGWRSIRVSLEREDIFRVQLAGILLASLHGKARIMF
EEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHCCCEEEHH
PMVSSIAEIWQIKKILGEVREELAHEGQTFDPDISFGIMVELPAAVQTAHILVNEVDFFS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCEEEE
IGTNDLIQYTMAADRNNPKVRRYYDSCQPAVLHSIKRVVDVALATGKPVSVCGEMAADPV
CCCHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
MAILLVGMGIRELSLSAPSIPVVKQAIRSVDLRTAREMADRVLSLVSSGAVRTYLAETQN
HHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ALGL
HCCC
>Mature Secondary Structure 
AEEQLETTGLRTLEDISTLILKSHDLPDTLDNIVHLVAKRIGTDVCSIYLLEDDGKTLR
CCHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEE
LAASKGLSKSSVGKITMQTSEGLAGLVIETKGLVTADNAPLHPRYKYFPETREERFNSFL
EEECCCCCCCCCCEEEEECCCCCEEEEEEECCEEEECCCCCCCCCCCCCCHHHHHHHHHC
GIPLMLRESPMGVIVIQTRESRTFQQEEVSTLTTIAYQISSIVINAKLLDSIRLKEAERE
CCCEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLERELEKFKAIGAPAGAEPSGDKRKREKRSLLLMGTPVSAGFSRGRAVIIDRRDEHDGM
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCEEEEECCCCCCCCCCCEEEEECCCCCCCC
GVATVRSRPEERSRFQTALEKAKIETICMEKRVAELLSKEDAAIFHTHLMILEDRGFIAK
CEEHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCHHHH
VHDLIEEDYGAVRAVNEVVAHYVSAFSRMEDPYLKERSADMEDIRRRIIAFLDGSGHARL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHCCCCCCEEE
RLREKRILVAHEILPSDMATLDHEKLLGIVTEKGDLNSHAAIMAKSLGIPAVVGVEGILQ
EEECCCEEHHHHHCCCHHHHCCHHHHHEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHH
KLGLRDEVIVDGNSGRIYINPSEKIKGEYERLEIDFGVRRRELEGLRDLPAVTRDGVDVA
HCCCCCEEEEECCCCEEEECCCHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCEE
LKANIGLLSDIRVALANGAEGVGLYRTEFPYMTRSAFPTRSELVGLYRKVLEGFAPHPVT
EECCCCCHHHHHHHHHCCCCCCCEEEECCCCHHCCCCCCHHHHHHHHHHHHHCCCCCCEE
IRTLDIGGDKTLPYFPMAAEDNPFMGWRSIRVSLEREDIFRVQLAGILLASLHGKARIMF
EEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHCCCEEEHH
PMVSSIAEIWQIKKILGEVREELAHEGQTFDPDISFGIMVELPAAVQTAHILVNEVDFFS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCEEEE
IGTNDLIQYTMAADRNNPKVRRYYDSCQPAVLHSIKRVVDVALATGKPVSVCGEMAADPV
CCCHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
MAILLVGMGIRELSLSAPSIPVVKQAIRSVDLRTAREMADRVLSLVSSGAVRTYLAETQN
HHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ALGL
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1551842; 1901791 [H]