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The map label for this gene is arnB [H]

Identifier: 78223535

GI number: 78223535

Start: 2639462

End: 2640676

Strand: Reverse

Name: arnB [H]

Synonym: Gmet_2332

Alternate gene names: 78223535

Gene position: 2640676-2639462 (Counterclockwise)

Preceding gene: 78223536

Following gene: 78223534

Centisome position: 66.06

GC content: 54.24

Gene sequence:

>1215_bases
ATGAAACTGAAGACTTCTTGTGATGAGCTTGCTGTTATGGGAGGTTCTCCGGCCTTTTCGGATACTCTCCACGTGGGCCG
GCCCAACATTGGAGACCGCTCTGAGTTTTTGGAAAGGGTCAACGGCATCCTTGACCGGCGCTGGCTCACCAATAACGGCA
TCTGCGTCCAGGAGTTCGAGCGGCGGCTTGAGGCGTTGCTGGGGGTCAAACACTGTATCGCCATGTGCAATGCAACGGTG
GCTCTGGAAATTACCATCAGAGCCCTGGGGATGACGGGGGAAGTGATCGTTCCTTCATTTACGTTTGTCGCCACTCCCCA
TTCACTCCAGTGGCAGCAGATTACCCCCGTTTTCTGTGATATCGATCCAGAAACACACTCCATCGACCCGCGTCGGGTCG
AGTCGCTCATTACCCCCCGCACGACAGGAATCATTGGTGTTCATCTGTGGGGGAAGCCGTGCAAGATCGAGGCGTTGGCT
GAGATCGCCGGTAGGCGCAATCTGAGATTGCTCTTCGATGCGGCACATGCGTTCGGCTGCTCCCATAACGGACGCATGAT
AGGAAGTTTCGGCGATGCCGAGGTCTTCAGCTTTCACGCGACGAAATTCTTCAATACCTTCGAGGGGGGAGCGGTTGCCA
CCAACGACGATGACCTGGCCGCACGGATCAGACTGATGAAGAATTTCGGGTTTGCGGGCTACGATAACGTCGTCTATATC
GGGACGAACGGCAAGATGTCTGAAATCTCGGCGGCAATGGGGCTCACATCGCTCGATCATTTGGAGGAAATCATTACCAT
CAATCGCCGAAACTATGCAGCTTATGCCAGTGGCCTTGCCAATGTGCAAGGCATTCGCCTCGTCCCCTATGAGGGGGCTG
ATCGCTACAACTACCAGTATGTGGTCCTTGAGGTAGATGAGCAGGCTGCGGGCATCCACCGCGACATGCTGCTGCAGGCG
CTTCATGCCGAAAATATCATGGCTCGGCGATATTTCTATCCCGGCTGCCATCGTATGGAGCCGTACCGGTCATATTTCCC
GAATGCCGGCATGCTGCTTCCTGAGACCGAACGACTTGCTGAGCGAGTATTGTCACTGCCGACGGGAACAGCCGTATCGG
AAGAGGATATTGTCGATATCTGCTCAATTATTCGGCTTGTACTAAAGAATGGGGTTGAAGTGACTAACGCCTTGGCCAAT
GGTAAAAACACGTAA

Upstream 100 bases:

>100_bases
CAGGCTCGTTCATAGGCGCCGGTTCGGTTGTTGTGGGCGATATTCCGGACAACGCGCTTGCGTACGGTGTGCCGGCAAAA
ATCAGAAAAAGGATCGAATA

Downstream 100 bases:

>100_bases
CCCGGAACTCAATCCCATGCCGCTCCGTGTCCTCATCGTCAAAGTCTCCGCCCTCGGCGACGTCATCCACGCCCTGCCGG
TCCTCGACTATCTCCACCAG

Product: DegT/DnrJ/EryC1/StrS aminotransferase

Products: NA

Alternate protein names: UDP-(beta-L-threo-pentapyranosyl-4''-ulose diphosphate) aminotransferase; UDP-Ara4O aminotransferase; UDP-4-amino-4-deoxy-L-arabinose aminotransferase [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKLKTSCDELAVMGGSPAFSDTLHVGRPNIGDRSEFLERVNGILDRRWLTNNGICVQEFERRLEALLGVKHCIAMCNATV
ALEITIRALGMTGEVIVPSFTFVATPHSLQWQQITPVFCDIDPETHSIDPRRVESLITPRTTGIIGVHLWGKPCKIEALA
EIAGRRNLRLLFDAAHAFGCSHNGRMIGSFGDAEVFSFHATKFFNTFEGGAVATNDDDLAARIRLMKNFGFAGYDNVVYI
GTNGKMSEISAAMGLTSLDHLEEIITINRRNYAAYASGLANVQGIRLVPYEGADRYNYQYVVLEVDEQAAGIHRDMLLQA
LHAENIMARRYFYPGCHRMEPYRSYFPNAGMLLPETERLAERVLSLPTGTAVSEEDIVDICSIIRLVLKNGVEVTNALAN
GKNT

Sequences:

>Translated_404_residues
MKLKTSCDELAVMGGSPAFSDTLHVGRPNIGDRSEFLERVNGILDRRWLTNNGICVQEFERRLEALLGVKHCIAMCNATV
ALEITIRALGMTGEVIVPSFTFVATPHSLQWQQITPVFCDIDPETHSIDPRRVESLITPRTTGIIGVHLWGKPCKIEALA
EIAGRRNLRLLFDAAHAFGCSHNGRMIGSFGDAEVFSFHATKFFNTFEGGAVATNDDDLAARIRLMKNFGFAGYDNVVYI
GTNGKMSEISAAMGLTSLDHLEEIITINRRNYAAYASGLANVQGIRLVPYEGADRYNYQYVVLEVDEQAAGIHRDMLLQA
LHAENIMARRYFYPGCHRMEPYRSYFPNAGMLLPETERLAERVLSLPTGTAVSEEDIVDICSIIRLVLKNGVEVTNALAN
GKNT
>Mature_404_residues
MKLKTSCDELAVMGGSPAFSDTLHVGRPNIGDRSEFLERVNGILDRRWLTNNGICVQEFERRLEALLGVKHCIAMCNATV
ALEITIRALGMTGEVIVPSFTFVATPHSLQWQQITPVFCDIDPETHSIDPRRVESLITPRTTGIIGVHLWGKPCKIEALA
EIAGRRNLRLLFDAAHAFGCSHNGRMIGSFGDAEVFSFHATKFFNTFEGGAVATNDDDLAARIRLMKNFGFAGYDNVVYI
GTNGKMSEISAAMGLTSLDHLEEIITINRRNYAAYASGLANVQGIRLVPYEGADRYNYQYVVLEVDEQAAGIHRDMLLQA
LHAENIMARRYFYPGCHRMEPYRSYFPNAGMLLPETERLAERVLSLPTGTAVSEEDIVDICSIIRLVLKNGVEVTNALAN
GKNT

Specific function: Catalyzes the conversion of UDP-4-keto-arabinose (UDP- Ara4O) to UDP-4-amino-4-deoxy-L-arabinose (UDP-L-Ara4N). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family. ArnB subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693159, Length=365, Percent_Identity=35.3424657534247, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI2367285, Length=360, Percent_Identity=27.7777777777778, Blast_Score=105, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022850
- InterPro:   IPR000653
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: =2.6.1.87 [H]

Molecular weight: Translated: 44748; Mature: 44748

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKTSCDELAVMGGSPAFSDTLHVGRPNIGDRSEFLERVNGILDRRWLTNNGICVQEFE
CCCCCCCCHHEEECCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHCCEECCCCEEHHHHH
RRLEALLGVKHCIAMCNATVALEITIRALGMTGEVIVPSFTFVATPHSLQWQQITPVFCD
HHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCEEECCEEEEECCCCCCCCCCCCEEEE
IDPETHSIDPRRVESLITPRTTGIIGVHLWGKPCKIEALAEIAGRRNLRLLFDAAHAFGC
CCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEEHHHHHCC
SHNGRMIGSFGDAEVFSFHATKFFNTFEGGAVATNDDDLAARIRLMKNFGFAGYDNVVYI
CCCCEEEECCCCCHHEEEHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCEEEE
GTNGKMSEISAAMGLTSLDHLEEIITINRRNYAAYASGLANVQGIRLVPYEGADRYNYQY
ECCCCHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEECCCCCCCCEEE
VVLEVDEQAAGIHRDMLLQALHAENIMARRYFYPGCHRMEPYRSYFPNAGMLLPETERLA
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCHHHHH
ERVLSLPTGTAVSEEDIVDICSIIRLVLKNGVEVTNALANGKNT
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCC
>Mature Secondary Structure
MKLKTSCDELAVMGGSPAFSDTLHVGRPNIGDRSEFLERVNGILDRRWLTNNGICVQEFE
CCCCCCCCHHEEECCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHCCEECCCCEEHHHHH
RRLEALLGVKHCIAMCNATVALEITIRALGMTGEVIVPSFTFVATPHSLQWQQITPVFCD
HHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCEEECCEEEEECCCCCCCCCCCCEEEE
IDPETHSIDPRRVESLITPRTTGIIGVHLWGKPCKIEALAEIAGRRNLRLLFDAAHAFGC
CCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEEHHHHHCC
SHNGRMIGSFGDAEVFSFHATKFFNTFEGGAVATNDDDLAARIRLMKNFGFAGYDNVVYI
CCCCEEEECCCCCHHEEEHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCEEEE
GTNGKMSEISAAMGLTSLDHLEEIITINRRNYAAYASGLANVQGIRLVPYEGADRYNYQY
ECCCCHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEECCCCCCCCEEE
VVLEVDEQAAGIHRDMLLQALHAENIMARRYFYPGCHRMEPYRSYFPNAGMLLPETERLA
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCHHHHH
ERVLSLPTGTAVSEEDIVDICSIIRLVLKNGVEVTNALANGKNT
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA