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The map label for this gene is guaA

Identifier: 78223495

GI number: 78223495

Start: 2597752

End: 2599314

Strand: Reverse

Name: guaA

Synonym: Gmet_2292

Alternate gene names: 78223495

Gene position: 2599314-2597752 (Counterclockwise)

Preceding gene: 78223496

Following gene: 78223494

Centisome position: 65.02

GC content: 62.32

Gene sequence:

>1563_bases
ATGAGCAGTGATATCCACAGCGAGAAAATCCTGATCCTGGACTTCGGTTCACAGTACACCCAACTCATCGCCCGGCGAGT
GCGGGAGGCCCACGTCTACTGCGAACTCCATCCCTTCGATATGGACCTGGCGGCGATCCGCGCCTTTGCCCCGCGGGGGA
TCATCCTCTCCGGCGGCCCCAAGTCGGTGTATGAGGAAGGGGCCCCGGCGGTGGAGGAGGCTCTTTTCGAGCTGGGAGTG
CCGGTCCTTGGCATCTGCTACGGCATGCAGCTCATGAGCCGCCACTTTGGCGGCCAGGTGGTGCCGGCCGGGAAACGGGA
GTTCGGCCACGCTGACCTCCTGGCTGTGGGGACTCCCGGCCCCCTCTTCGATGGCTTTTTCGTGGAAGGGAAAAGCCCGG
TCTGGATGAGCCATGGCGACCATGTGTCTCTGGTTCCCGCAGGGTTTCAGGTGGTGGGGGAGACGGCCAACGCGCCGGTC
TGCGCCATCCAGGATCTTGCCCGCAATCTCTATGGTGTCCAGTTTCACCCGGAAGTTAACCATACCCCTCGGGGGGAACT
CCTCATCGATACCTTTGTCCGGAAGATCTGCGGTTGCAGTGGCCAGTGGACGCCGGGACACATTATCGATGATGCCGTCA
GCCGTATCCGCGCCCAGGTGGGGAATGACCGGGTGATCCTCGGCCTTTCGGGCGGAGTCGACTCGTCGGTGGCCGCAGCC
CTGATCCATCGCGCCATCGGCGACCAGCTCACCTGTGTTTTCGTGGACAACGGCCTGTTGCGCCTCAGCGAGGGGGACCA
GGTGATGGCCACCTTTGCCGAGAATCTCGGAGTCAAGGTGATACGGGTGGACGCCGAGGATCGCTTTCTCACGGCCCTGG
CCGGCGAGAGCGACCCGGAGAAAAAACGCAAGATCATCGGCAAACTCTTCGTGGATATCTTCGAGGAAGAGTCGAATAAG
ATCACCGACGCCCGCTGGCTTGCCCAAGGGACCATCTACCCCGACGTCATCGAGAGCGCCGGAGCCAAGACCGGCAAGGC
CCACAACATCAAGAGCCACCACAACGTGGGGGGACTCCCCGACTACATGAAGCTGAAGCTCCTGGAGCCCCTGCGGGAGC
TCTTCAAGGATGAGGTCCGGGCCATCGGCGAGGAGCTGGGGCTTCCCCATCAGATGGTATGGCGCCATCCCTTCCCGGGG
CCGGGGCTTGGGGTCAGGATCCTTGGCGAGGTGAAGAAGGAGTACGCCGACATCCTCCGCCGGGCCGACGCCATCTACAT
CGAGGAGCTTTACGCTGCCGGCCACTACGACAAGATCAGTCAGGCTTTCGCCGTCTTCCTGCCGGTTAAGAGCGTCGGGG
TCATGGGTGACGGTCGCACCTACGAGTACGTGGTGGCGCTGCGGGCCGTGGAGACCAAGGACTTCATGACCGCAGGGTGG
TATCCGCTTCCCTACGAGGACATGGCCCGCATATCGAGCAGGATCATCAACGAGGTGAAGGGGGTCAATCGGGTGGTGTA
CGACATCTCCAGCAAGCCTCCCGCTACCATAGAGTGGGAATAG

Upstream 100 bases:

>100_bases
GGCGCTTCATCCGGATCACCGGCGCCGGGCTCAAGGAGTCCCACGTCCACGACGTCATGATTACCAAGGAAGCTCCCAAC
TACCGGGTTGAGAACGTACG

Downstream 100 bases:

>100_bases
ATTCATTTCAGAAAGGGCGCCGAACGGCGCCTTTTTCTTTTTTTCGGACCCACCTGAAAATACGGAGTGATTTGGTATTA
TTTCAGTAGAGAGAGTAAAT

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MSSDIHSEKILILDFGSQYTQLIARRVREAHVYCELHPFDMDLAAIRAFAPRGIILSGGPKSVYEEGAPAVEEALFELGV
PVLGICYGMQLMSRHFGGQVVPAGKREFGHADLLAVGTPGPLFDGFFVEGKSPVWMSHGDHVSLVPAGFQVVGETANAPV
CAIQDLARNLYGVQFHPEVNHTPRGELLIDTFVRKICGCSGQWTPGHIIDDAVSRIRAQVGNDRVILGLSGGVDSSVAAA
LIHRAIGDQLTCVFVDNGLLRLSEGDQVMATFAENLGVKVIRVDAEDRFLTALAGESDPEKKRKIIGKLFVDIFEEESNK
ITDARWLAQGTIYPDVIESAGAKTGKAHNIKSHHNVGGLPDYMKLKLLEPLRELFKDEVRAIGEELGLPHQMVWRHPFPG
PGLGVRILGEVKKEYADILRRADAIYIEELYAAGHYDKISQAFAVFLPVKSVGVMGDGRTYEYVVALRAVETKDFMTAGW
YPLPYEDMARISSRIINEVKGVNRVVYDISSKPPATIEWE

Sequences:

>Translated_520_residues
MSSDIHSEKILILDFGSQYTQLIARRVREAHVYCELHPFDMDLAAIRAFAPRGIILSGGPKSVYEEGAPAVEEALFELGV
PVLGICYGMQLMSRHFGGQVVPAGKREFGHADLLAVGTPGPLFDGFFVEGKSPVWMSHGDHVSLVPAGFQVVGETANAPV
CAIQDLARNLYGVQFHPEVNHTPRGELLIDTFVRKICGCSGQWTPGHIIDDAVSRIRAQVGNDRVILGLSGGVDSSVAAA
LIHRAIGDQLTCVFVDNGLLRLSEGDQVMATFAENLGVKVIRVDAEDRFLTALAGESDPEKKRKIIGKLFVDIFEEESNK
ITDARWLAQGTIYPDVIESAGAKTGKAHNIKSHHNVGGLPDYMKLKLLEPLRELFKDEVRAIGEELGLPHQMVWRHPFPG
PGLGVRILGEVKKEYADILRRADAIYIEELYAAGHYDKISQAFAVFLPVKSVGVMGDGRTYEYVVALRAVETKDFMTAGW
YPLPYEDMARISSRIINEVKGVNRVVYDISSKPPATIEWE
>Mature_519_residues
SSDIHSEKILILDFGSQYTQLIARRVREAHVYCELHPFDMDLAAIRAFAPRGIILSGGPKSVYEEGAPAVEEALFELGVP
VLGICYGMQLMSRHFGGQVVPAGKREFGHADLLAVGTPGPLFDGFFVEGKSPVWMSHGDHVSLVPAGFQVVGETANAPVC
AIQDLARNLYGVQFHPEVNHTPRGELLIDTFVRKICGCSGQWTPGHIIDDAVSRIRAQVGNDRVILGLSGGVDSSVAAAL
IHRAIGDQLTCVFVDNGLLRLSEGDQVMATFAENLGVKVIRVDAEDRFLTALAGESDPEKKRKIIGKLFVDIFEEESNKI
TDARWLAQGTIYPDVIESAGAKTGKAHNIKSHHNVGGLPDYMKLKLLEPLRELFKDEVRAIGEELGLPHQMVWRHPFPGP
GLGVRILGEVKKEYADILRRADAIYIEELYAAGHYDKISQAFAVFLPVKSVGVMGDGRTYEYVVALRAVETKDFMTAGWY
PLPYEDMARISSRIINEVKGVNRVVYDISSKPPATIEWE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=551, Percent_Identity=38.2940108892922, Blast_Score=322, Evalue=8e-88,
Organism=Escherichia coli, GI1788854, Length=525, Percent_Identity=63.4285714285714, Blast_Score=689, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71992717, Length=571, Percent_Identity=37.1278458844133, Blast_Score=318, Evalue=5e-87,
Organism=Caenorhabditis elegans, GI71992710, Length=571, Percent_Identity=37.1278458844133, Blast_Score=318, Evalue=6e-87,
Organism=Caenorhabditis elegans, GI133901714, Length=571, Percent_Identity=37.1278458844133, Blast_Score=317, Evalue=8e-87,
Organism=Saccharomyces cerevisiae, GI6323873, Length=519, Percent_Identity=52.7938342967245, Blast_Score=563, Evalue=1e-161,
Organism=Drosophila melanogaster, GI281365319, Length=552, Percent_Identity=37.1376811594203, Blast_Score=320, Evalue=1e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_GEOMG (Q39TA7)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_385242.1
- ProteinModelPortal:   Q39TA7
- SMR:   Q39TA7
- STRING:   Q39TA7
- GeneID:   3739320
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_2292
- NMPDR:   fig|269799.3.peg.2536
- eggNOG:   COG0519
- HOGENOM:   HBG391775
- OMA:   MKLVEPL
- PhylomeDB:   Q39TA7
- ProtClustDB:   PRK00074
- BioCyc:   GMET269799:GMET_2292-MONOMER
- HAMAP:   MF_00344
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00884
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase

EC number: =6.3.5.2

Molecular weight: Translated: 57141; Mature: 57010

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 86-86 ACT_SITE 176-176 ACT_SITE 178-178

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDIHSEKILILDFGSQYTQLIARRVREAHVYCELHPFDMDLAAIRAFAPRGIILSGGP
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHCCCCEEEECCC
KSVYEEGAPAVEEALFELGVPVLGICYGMQLMSRHFGGQVVPAGKREFGHADLLAVGTPG
HHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCEEEECCCC
PLFDGFFVEGKSPVWMSHGDHVSLVPAGFQVVGETANAPVCAIQDLARNLYGVQFHPEVN
CCCCCEEECCCCCEEECCCCEEEEECCCCEEECCCCCCCHHHHHHHHHHHCCEEECCCCC
HTPRGELLIDTFVRKICGCSGQWTPGHIIDDAVSRIRAQVGNDRVILGLSGGVDSSVAAA
CCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
LIHRAIGDQLTCVFVDNGLLRLSEGDQVMATFAENLGVKVIRVDAEDRFLTALAGESDPE
HHHHHHCCCEEEEEECCCEEEECCCCHHHHHHHHHCCEEEEEECCCCCEEEEECCCCCHH
KKRKIIGKLFVDIFEEESNKITDARWLAQGTIYPDVIESAGAKTGKAHNIKSHHNVGGLP
HHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCC
DYMKLKLLEPLRELFKDEVRAIGEELGLPHQMVWRHPFPGPGLGVRILGEVKKEYADILR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
RADAIYIEELYAAGHYDKISQAFAVFLPVKSVGVMGDGRTYEYVVALRAVETKDFMTAGW
HCCHHHHHHHHHCCCHHHHHHHHHHEEEHHHHCCCCCCCCEEEEEEEEHHCCCCHHCCCC
YPLPYEDMARISSRIINEVKGVNRVVYDISSKPPATIEWE
CCCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCEEECC
>Mature Secondary Structure 
SSDIHSEKILILDFGSQYTQLIARRVREAHVYCELHPFDMDLAAIRAFAPRGIILSGGP
CCCCCCCEEEEEECCCHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHCCCCEEEECCC
KSVYEEGAPAVEEALFELGVPVLGICYGMQLMSRHFGGQVVPAGKREFGHADLLAVGTPG
HHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCEEEECCCC
PLFDGFFVEGKSPVWMSHGDHVSLVPAGFQVVGETANAPVCAIQDLARNLYGVQFHPEVN
CCCCCEEECCCCCEEECCCCEEEEECCCCEEECCCCCCCHHHHHHHHHHHCCEEECCCCC
HTPRGELLIDTFVRKICGCSGQWTPGHIIDDAVSRIRAQVGNDRVILGLSGGVDSSVAAA
CCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
LIHRAIGDQLTCVFVDNGLLRLSEGDQVMATFAENLGVKVIRVDAEDRFLTALAGESDPE
HHHHHHCCCEEEEEECCCEEEECCCCHHHHHHHHHCCEEEEEECCCCCEEEEECCCCCHH
KKRKIIGKLFVDIFEEESNKITDARWLAQGTIYPDVIESAGAKTGKAHNIKSHHNVGGLP
HHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCC
DYMKLKLLEPLRELFKDEVRAIGEELGLPHQMVWRHPFPGPGLGVRILGEVKKEYADILR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
RADAIYIEELYAAGHYDKISQAFAVFLPVKSVGVMGDGRTYEYVVALRAVETKDFMTAGW
HCCHHHHHHHHHCCCHHHHHHHHHHEEEHHHHCCCCCCCCEEEEEEEEHHCCCCHHCCCC
YPLPYEDMARISSRIINEVKGVNRVVYDISSKPPATIEWE
CCCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA