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The map label for this gene is fhlA [H]

Identifier: 78223428

GI number: 78223428

Start: 2513938

End: 2515608

Strand: Reverse

Name: fhlA [H]

Synonym: Gmet_2223

Alternate gene names: 78223428

Gene position: 2515608-2513938 (Counterclockwise)

Preceding gene: 78223432

Following gene: 78223423

Centisome position: 62.93

GC content: 55.72

Gene sequence:

>1671_bases
ATGGAGAAACCTGGAGGAAGCGGAAACAGGGGGGGTGCGACGCAGGGAGAGAGCAATCCTCCGGTAGACTGTTCTTCTTG
TGGTCCAAACAGCAGCACGTTGGAAGAACGTCTGAGATTCGAGATGCTTCTTTCGGAGATATCTTCAGTCTATTCCAATC
TCCCTGTGGAAGAGGTGGATAAGACGATTGAGTCTGGTCTCCAGAGGCTTGGTGAATTTTTGGGGGCCGACCGATGCAAC
CTGGTGCAATTCTCCGAAGACGGTTCCCGCGTCCTTGTTTTTCATTCGTGGATACACGATGACGCAAAGCCTGACCCCGG
GTTTATCGCCCCCGATCATAATCTTTTCCCCTGGTTTGCGAAGATGTTTCATCTTGGGAAGGTCGTACAGTTTTCCCATC
TGGATGACCTGCCGGCGGAGGCGGCGACTGATATAACGGCACTGCTGGCATTGCGCATAAAGTCGCAGGTGTCGGTTCCG
ATCTCGGTAGGGGGGCATGTCTTCGGGACTCTTTCCCTTGCGACGGTCCGGAGCTACCGTTCCTGGCCGGATGAACTGGT
GCCACGGTTGCGTCTTGTGGGGGAAATCTTTGCCAACGCCCTGGTAAGGAAGCAGAAAGAACTGCAGATTCGCAAAGCGT
TTCAGCAGATCAAGGAACTGAACGAGGAACTTGCGGCTGACTGCACGTATTTGCGGGAGGAGATCGATCTGAACTACGAT
GTGCATCATATCATCGGCCAGAGTCTTCCCCTCAAACGGGTGTTGGCCAAGATCCAGCAGATTGCCCTTACGGACACGAC
CGTCCTCATACTGGGGGAAACCGGCACTGGCAAGGAACTGGTTGCCCGGGCGATTCATGCGGCGAGCCAGCGTCGAGATC
GGCCGATGGTTAAGGTGAACTGTGCCTCGCTTCCTGCAAATCTTATAGAGAGCGAGTTGTTCGGCCACGAGAAGGGTGCG
TTTACCAGTGCCCAGGTGAGGCAGATCGGGCGTTTCGAGTTTGCCGACCGCAATACCCTCTTTCTCGACGAGATCGGTGA
ACTTCCTCTGGAATCCCAGGCGAAACTCCTCCGGGTGCTCCAGGAAGGAGAGTTCGAGAGGTTGGGCAGTTCGCGCACGT
TAAAGGTCGATGTCCGTATCGTTGCCGCCACCAACAGGGACCTGGAGGAAGAGGTCCGCAAGGGGCGTTTTCGTCAGGAT
CTGTGGTATCGCCTCAACATCTTTCCCATCACGGTTCCGCCGCTGCGTGAGCGTACGGAAGATATCCCCCTGCTCATCAA
TCTCTTCGTGAACAAATTCGGCCGGAAACTGGGGAAGAGTATTCGGCGCATTCCCGCAGGTGTGATGAAGTCCTTACAGG
CCTACGATTGGCCGGGGAACGTGCGGGAACTGGAAAACGTCATCGAGCGTGCGGCGATCAATACCGTCGGCCCATCCCTC
CAACTGCTCGATAACCTGAAGAGTTCCTTGGTAACGGAAAAGACCGCAGCACCACGGCTGACCCTGGAAGAAGCCGAACG
GAGCCATATCCTGCAGGTGCTTGCGGATACGAACGGCCGTATAGAGGGACCCAAGGGGGCCGCATTGATACTCGGTATCA
ACCACTCGACTCTTCGTGCCAGGATGCGCAAGCTCGGCATTCACATCCGAAGGATTTCAGACGCCGATTGA

Upstream 100 bases:

>100_bases
TTCCTGTTGTATTTAACAGTAGTGTAACAATTGAAAGAAAAGTTTCGCTGGAAAGCGCTGTCGTGGGTGGCAGCGATGTC
GTTAGCCGAGGAGGCGATGA

Downstream 100 bases:

>100_bases
GCTGCAATGCAGGTGTAACGAGGCGTTGATTACAGGCGTTGGCAGAGCTTTTTTCGCAATATCGCAGTCAATTTTCCGGT
AACGTCCTTTGCCGGTCCCT

Product: NifA subfamily transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MEKPGGSGNRGGATQGESNPPVDCSSCGPNSSTLEERLRFEMLLSEISSVYSNLPVEEVDKTIESGLQRLGEFLGADRCN
LVQFSEDGSRVLVFHSWIHDDAKPDPGFIAPDHNLFPWFAKMFHLGKVVQFSHLDDLPAEAATDITALLALRIKSQVSVP
ISVGGHVFGTLSLATVRSYRSWPDELVPRLRLVGEIFANALVRKQKELQIRKAFQQIKELNEELAADCTYLREEIDLNYD
VHHIIGQSLPLKRVLAKIQQIALTDTTVLILGETGTGKELVARAIHAASQRRDRPMVKVNCASLPANLIESELFGHEKGA
FTSAQVRQIGRFEFADRNTLFLDEIGELPLESQAKLLRVLQEGEFERLGSSRTLKVDVRIVAATNRDLEEEVRKGRFRQD
LWYRLNIFPITVPPLRERTEDIPLLINLFVNKFGRKLGKSIRRIPAGVMKSLQAYDWPGNVRELENVIERAAINTVGPSL
QLLDNLKSSLVTEKTAAPRLTLEEAERSHILQVLADTNGRIEGPKGAALILGINHSTLRARMRKLGIHIRRISDAD

Sequences:

>Translated_556_residues
MEKPGGSGNRGGATQGESNPPVDCSSCGPNSSTLEERLRFEMLLSEISSVYSNLPVEEVDKTIESGLQRLGEFLGADRCN
LVQFSEDGSRVLVFHSWIHDDAKPDPGFIAPDHNLFPWFAKMFHLGKVVQFSHLDDLPAEAATDITALLALRIKSQVSVP
ISVGGHVFGTLSLATVRSYRSWPDELVPRLRLVGEIFANALVRKQKELQIRKAFQQIKELNEELAADCTYLREEIDLNYD
VHHIIGQSLPLKRVLAKIQQIALTDTTVLILGETGTGKELVARAIHAASQRRDRPMVKVNCASLPANLIESELFGHEKGA
FTSAQVRQIGRFEFADRNTLFLDEIGELPLESQAKLLRVLQEGEFERLGSSRTLKVDVRIVAATNRDLEEEVRKGRFRQD
LWYRLNIFPITVPPLRERTEDIPLLINLFVNKFGRKLGKSIRRIPAGVMKSLQAYDWPGNVRELENVIERAAINTVGPSL
QLLDNLKSSLVTEKTAAPRLTLEEAERSHILQVLADTNGRIEGPKGAALILGINHSTLRARMRKLGIHIRRISDAD
>Mature_556_residues
MEKPGGSGNRGGATQGESNPPVDCSSCGPNSSTLEERLRFEMLLSEISSVYSNLPVEEVDKTIESGLQRLGEFLGADRCN
LVQFSEDGSRVLVFHSWIHDDAKPDPGFIAPDHNLFPWFAKMFHLGKVVQFSHLDDLPAEAATDITALLALRIKSQVSVP
ISVGGHVFGTLSLATVRSYRSWPDELVPRLRLVGEIFANALVRKQKELQIRKAFQQIKELNEELAADCTYLREEIDLNYD
VHHIIGQSLPLKRVLAKIQQIALTDTTVLILGETGTGKELVARAIHAASQRRDRPMVKVNCASLPANLIESELFGHEKGA
FTSAQVRQIGRFEFADRNTLFLDEIGELPLESQAKLLRVLQEGEFERLGSSRTLKVDVRIVAATNRDLEEEVRKGRFRQD
LWYRLNIFPITVPPLRERTEDIPLLINLFVNKFGRKLGKSIRRIPAGVMKSLQAYDWPGNVRELENVIERAAINTVGPSL
QLLDNLKSSLVTEKTAAPRLTLEEAERSHILQVLADTNGRIEGPKGAALILGINHSTLRARMRKLGIHIRRISDAD

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=405, Percent_Identity=48.3950617283951, Blast_Score=353, Evalue=2e-98,
Organism=Escherichia coli, GI87082117, Length=413, Percent_Identity=46.9733656174334, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI1788550, Length=354, Percent_Identity=44.3502824858757, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI1790437, Length=304, Percent_Identity=47.0394736842105, Blast_Score=262, Evalue=5e-71,
Organism=Escherichia coli, GI87082152, Length=361, Percent_Identity=42.1052631578947, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI1789233, Length=324, Percent_Identity=40.4320987654321, Blast_Score=235, Evalue=6e-63,
Organism=Escherichia coli, GI1790299, Length=341, Percent_Identity=40.4692082111437, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1788905, Length=283, Percent_Identity=44.1696113074205, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI87081872, Length=320, Percent_Identity=40.625, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1786524, Length=335, Percent_Identity=40.5970149253731, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI1787583, Length=313, Percent_Identity=37.3801916932907, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI87081858, Length=303, Percent_Identity=32.3432343234323, Blast_Score=148, Evalue=8e-37,
Organism=Escherichia coli, GI1789828, Length=232, Percent_Identity=38.3620689655172, Blast_Score=143, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 62094; Mature: 62094

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKPGGSGNRGGATQGESNPPVDCSSCGPNSSTLEERLRFEMLLSEISSVYSNLPVEEVD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
KTIESGLQRLGEFLGADRCNLVQFSEDGSRVLVFHSWIHDDAKPDPGFIAPDHNLFPWFA
HHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEEECCCCCCCCCCEECCCCCCHHHHH
KMFHLGKVVQFSHLDDLPAEAATDITALLALRIKSQVSVPISVGGHVFGTLSLATVRSYR
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHH
SWPDELVPRLRLVGEIFANALVRKQKELQIRKAFQQIKELNEELAADCTYLREEIDLNYD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VHHIIGQSLPLKRVLAKIQQIALTDTTVLILGETGTGKELVARAIHAASQRRDRPMVKVN
HHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEE
CASLPANLIESELFGHEKGAFTSAQVRQIGRFEFADRNTLFLDEIGELPLESQAKLLRVL
HHHCCHHHHHHHHHCCCCCCCCHHHHHHHHCCEECCCCEEEHHHHCCCCCHHHHHHHHHH
QEGEFERLGSSRTLKVDVRIVAATNRDLEEEVRKGRFRQDLWYRLNIFPITVPPLRERTE
HCCCHHHCCCCCEEEEEEEEEEECCCCHHHHHHCCCHHHHHEEEEEEEEECCCCHHHCCC
DIPLLINLFVNKFGRKLGKSIRRIPAGVMKSLQAYDWPGNVRELENVIERAAINTVGPSL
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHH
QLLDNLKSSLVTEKTAAPRLTLEEAERSHILQVLADTNGRIEGPKGAALILGINHSTLRA
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCCEEEEECCHHHHHH
RMRKLGIHIRRISDAD
HHHHHCCEEEECCCCC
>Mature Secondary Structure
MEKPGGSGNRGGATQGESNPPVDCSSCGPNSSTLEERLRFEMLLSEISSVYSNLPVEEVD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
KTIESGLQRLGEFLGADRCNLVQFSEDGSRVLVFHSWIHDDAKPDPGFIAPDHNLFPWFA
HHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEEECCCCCCCCCCEECCCCCCHHHHH
KMFHLGKVVQFSHLDDLPAEAATDITALLALRIKSQVSVPISVGGHVFGTLSLATVRSYR
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHH
SWPDELVPRLRLVGEIFANALVRKQKELQIRKAFQQIKELNEELAADCTYLREEIDLNYD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VHHIIGQSLPLKRVLAKIQQIALTDTTVLILGETGTGKELVARAIHAASQRRDRPMVKVN
HHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEE
CASLPANLIESELFGHEKGAFTSAQVRQIGRFEFADRNTLFLDEIGELPLESQAKLLRVL
HHHCCHHHHHHHHHCCCCCCCCHHHHHHHHCCEECCCCEEEHHHHCCCCCHHHHHHHHHH
QEGEFERLGSSRTLKVDVRIVAATNRDLEEEVRKGRFRQDLWYRLNIFPITVPPLRERTE
HCCCHHHCCCCCEEEEEEEEEEECCCCHHHHHHCCCHHHHHEEEEEEEEECCCCHHHCCC
DIPLLINLFVNKFGRKLGKSIRRIPAGVMKSLQAYDWPGNVRELENVIERAAINTVGPSL
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHH
QLLDNLKSSLVTEKTAAPRLTLEEAERSHILQVLADTNGRIEGPKGAALILGINHSTLRA
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCCEEEEECCHHHHHH
RMRKLGIHIRRISDAD
HHHHHCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]