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The map label for this gene is mutB [H]

Identifier: 78223414

GI number: 78223414

Start: 2498972

End: 2500642

Strand: Reverse

Name: mutB [H]

Synonym: Gmet_2209

Alternate gene names: 78223414

Gene position: 2500642-2498972 (Counterclockwise)

Preceding gene: 78223415

Following gene: 78223413

Centisome position: 62.56

GC content: 56.19

Gene sequence:

>1671_bases
ATGAGTAGTCCTGTTGCTAATGAAAATTTTTCCAAGGTGAAAGAGGATTGGGAGAAAGCCCGGGCAAAATACGTTGCCAA
GCTGAAGCAACGGGAAAACCTGTCGGGCATCCCCCTGAAAACGGTCTATACCCAGGAAGACATCCAGGGGCTTGACCTGG
CAACCATGCCCGGGGTTTACCCCTATACCCGCGGCCTCTACCCCGACGGTTACGCCCTGACCCCCTGGATGCAGCAGATG
GTTTTCGGTTACGGGACAGTCGAGGAAACCAGGAAGAAGATGGAGACCCTGGTTGCCCAAGGGATGGAGGGGTATTTCGG
CAACAAGGTGTTCAACATGGTACTGGATATCCCGTCCATGTACGGCATCGATTCCGACCATCCCGAGGCCGAAGGCAACG
TCGGGCAATGCGGCGTCACCCTCTGTACCGTGGAAGACTATGATGACCTTATCCAGGGGTGGGATCTCAACACGACTAAT
TTTTCGCTGATAACCGGCGATAACTGTCTCCCCGCCCTGGCACTGATAACGGCAGCCGCAATCCGGCGCGGCGAGGAACC
CGGGGTTCTTCATGGCAACTCCATGAACTGGTATCCGCGGACCGCTGTTCAGGACATCCCTTCATGGGAGCCGAAAAATG
GCTATCTGCTTATGACCGACCTTATCAGGTACTGTTCCAACAATATCCCCGCCTGGAACACGGCCAACCTGTTCATGTAC
GGATTGTCAGAGGCTGGTGCCACGCCGGTCCAGGAACTGGCGTATGGTTTGGCCTGGGGCAAGTCGATTATCGATGCCTG
TAAGGAGCGTGGGCTTGAACCGGACAGTTTTGTTGGGCGCATGGGTTTCCAGATCGGCATCCAGATGAATTTCTTCGAGG
AGGTCGCCAAGCTGCGGGCCCATCGGCGGATGTGGGCGAAAATAACCAGAGACGCCGGCTGCACCAAGCCCCAGTGCATG
CATGCCCGGGTGCATGTCCATACCAGTGGCAATGTGCTGACCGCCCAGCAACCTCTCAACAACACGGCGCGCATCACCAT
GCAACTCATGGCGGCAGTCATGGGGGGGGTACAGTCGATACATTCCTGCTCCTACGACGAAACGATTGGCATCCCGACGG
AACTGTCGCACCGGACAGCCTTACGCTCCCAGCAGATCATGATGTGGGAGAGCGGGTTGCCGGATGTGGCAGATCCCCTG
GCAGGGTCCTATTACATTGAAGCATTAACCGATCAGATGGAGCAGGCTGCCTGGAAGATATACAACGAACTGGAAGAGCA
GGGAGGCTATAGGCAAGGTCTCGCTGACGGCAGCATCAAGCGGGCCATCGACAACAGCGCCTATGCAACCAAGGTCAAGA
TCAAGAGCGGCAAACAACCGGTGGTGGGCCTTAACAAGTATGTCATGGACAGAAAAGACAACTATGAACCATTCAAGGTT
GACACCGCTCTGGAAGTGAAGGCACTGGAAAGGTTGAAGGCGTTCAAGGCCAAACGGAACCAGGGGGATGTGGACAAGGC
CCTGGCAACGGTGAAGAATGCCTGTCTGGAACTGCGGGATGGCCAGGGTGAATTGATGCCGGTTCTGGTGGAAGCTGCGC
TGAAGGGGGCTACGAACGGCGAGATGTGCCAGGTGATGCGGGATGTGTTCGGTTGGGTAGTGACGGAATAG

Upstream 100 bases:

>100_bases
CGCCACGGAACAACAGATAGTCGACAAAATATCCTGGAATTAAGCTTCATGCCGACGCCACGGGTTCCCGCCCCATACGA
ATAGAAACGGAGGAGTAAAT

Downstream 100 bases:

>100_bases
GCTGGTTCTGTCGGTAACAGGGATTGTGCGCGCAGAGTCTACTACTTCAATGAAGATTGATAATTCGAGGTGAATGGTAT
GTCCAATCAACACATAAAAG

Product: methylmalonyl-CoA mutase-like

Products: NA

Alternate protein names: MCM-alpha [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MSSPVANENFSKVKEDWEKARAKYVAKLKQRENLSGIPLKTVYTQEDIQGLDLATMPGVYPYTRGLYPDGYALTPWMQQM
VFGYGTVEETRKKMETLVAQGMEGYFGNKVFNMVLDIPSMYGIDSDHPEAEGNVGQCGVTLCTVEDYDDLIQGWDLNTTN
FSLITGDNCLPALALITAAAIRRGEEPGVLHGNSMNWYPRTAVQDIPSWEPKNGYLLMTDLIRYCSNNIPAWNTANLFMY
GLSEAGATPVQELAYGLAWGKSIIDACKERGLEPDSFVGRMGFQIGIQMNFFEEVAKLRAHRRMWAKITRDAGCTKPQCM
HARVHVHTSGNVLTAQQPLNNTARITMQLMAAVMGGVQSIHSCSYDETIGIPTELSHRTALRSQQIMMWESGLPDVADPL
AGSYYIEALTDQMEQAAWKIYNELEEQGGYRQGLADGSIKRAIDNSAYATKVKIKSGKQPVVGLNKYVMDRKDNYEPFKV
DTALEVKALERLKAFKAKRNQGDVDKALATVKNACLELRDGQGELMPVLVEAALKGATNGEMCQVMRDVFGWVVTE

Sequences:

>Translated_556_residues
MSSPVANENFSKVKEDWEKARAKYVAKLKQRENLSGIPLKTVYTQEDIQGLDLATMPGVYPYTRGLYPDGYALTPWMQQM
VFGYGTVEETRKKMETLVAQGMEGYFGNKVFNMVLDIPSMYGIDSDHPEAEGNVGQCGVTLCTVEDYDDLIQGWDLNTTN
FSLITGDNCLPALALITAAAIRRGEEPGVLHGNSMNWYPRTAVQDIPSWEPKNGYLLMTDLIRYCSNNIPAWNTANLFMY
GLSEAGATPVQELAYGLAWGKSIIDACKERGLEPDSFVGRMGFQIGIQMNFFEEVAKLRAHRRMWAKITRDAGCTKPQCM
HARVHVHTSGNVLTAQQPLNNTARITMQLMAAVMGGVQSIHSCSYDETIGIPTELSHRTALRSQQIMMWESGLPDVADPL
AGSYYIEALTDQMEQAAWKIYNELEEQGGYRQGLADGSIKRAIDNSAYATKVKIKSGKQPVVGLNKYVMDRKDNYEPFKV
DTALEVKALERLKAFKAKRNQGDVDKALATVKNACLELRDGQGELMPVLVEAALKGATNGEMCQVMRDVFGWVVTE
>Mature_555_residues
SSPVANENFSKVKEDWEKARAKYVAKLKQRENLSGIPLKTVYTQEDIQGLDLATMPGVYPYTRGLYPDGYALTPWMQQMV
FGYGTVEETRKKMETLVAQGMEGYFGNKVFNMVLDIPSMYGIDSDHPEAEGNVGQCGVTLCTVEDYDDLIQGWDLNTTNF
SLITGDNCLPALALITAAAIRRGEEPGVLHGNSMNWYPRTAVQDIPSWEPKNGYLLMTDLIRYCSNNIPAWNTANLFMYG
LSEAGATPVQELAYGLAWGKSIIDACKERGLEPDSFVGRMGFQIGIQMNFFEEVAKLRAHRRMWAKITRDAGCTKPQCMH
ARVHVHTSGNVLTAQQPLNNTARITMQLMAAVMGGVQSIHSCSYDETIGIPTELSHRTALRSQQIMMWESGLPDVADPLA
GSYYIEALTDQMEQAAWKIYNELEEQGGYRQGLADGSIKRAIDNSAYATKVKIKSGKQPVVGLNKYVMDRKDNYEPFKVD
TALEVKALERLKAFKAKRNQGDVDKALATVKNACLELRDGQGELMPVLVEAALKGATNGEMCQVMRDVFGWVVTE

Specific function: Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates. This conversion most likely represents an important source of building blocks for polyketide antibiotic biosynthes

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=544, Percent_Identity=35.4779411764706, Blast_Score=311, Evalue=1e-84,
Organism=Escherichia coli, GI1789284, Length=513, Percent_Identity=33.5282651072125, Blast_Score=296, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI25144500, Length=550, Percent_Identity=34.1818181818182, Blast_Score=310, Evalue=9e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 61825; Mature: 61694

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPVANENFSKVKEDWEKARAKYVAKLKQRENLSGIPLKTVYTQEDIQGLDLATMPGVY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHCCCCCCCCCCCCCC
PYTRGLYPDGYALTPWMQQMVFGYGTVEETRKKMETLVAQGMEGYFGNKVFNMVLDIPSM
CHHCCCCCCCCEECHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHH
YGIDSDHPEAEGNVGQCGVTLCTVEDYDDLIQGWDLNTTNFSLITGDNCLPALALITAAA
CCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHH
IRRGEEPGVLHGNSMNWYPRTAVQDIPSWEPKNGYLLMTDLIRYCSNNIPAWNTANLFMY
HHCCCCCCEEECCCCCCCCCHHHHCCCCCCCCCCEEEHHHHHHHHCCCCCCCCCCCEEEE
GLSEAGATPVQELAYGLAWGKSIIDACKERGLEPDSFVGRMGFQIGIQMNFFEEVAKLRA
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEHHHHHHHHHHHH
HRRMWAKITRDAGCTKPQCMHARVHVHTSGNVLTAQQPLNNTARITMQLMAAVMGGVQSI
HHHHHHHHHHCCCCCCCCEEEEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHH
HSCSYDETIGIPTELSHRTALRSQQIMMWESGLPDVADPLAGSYYIEALTDQMEQAAWKI
HCCCCCCCCCCCCCHHHHHHHHHHHEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHHHH
YNELEEQGGYRQGLADGSIKRAIDNSAYATKVKIKSGKQPVVGLNKYVMDRKDNYEPFKV
HHHHHHCCCHHCCCCCCHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHCCCCCCCCEEE
DTALEVKALERLKAFKAKRNQGDVDKALATVKNACLELRDGQGELMPVLVEAALKGATNG
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCH
EMCQVMRDVFGWVVTE
HHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SSPVANENFSKVKEDWEKARAKYVAKLKQRENLSGIPLKTVYTQEDIQGLDLATMPGVY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHCCCCCCCCCCCCCC
PYTRGLYPDGYALTPWMQQMVFGYGTVEETRKKMETLVAQGMEGYFGNKVFNMVLDIPSM
CHHCCCCCCCCEECHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHH
YGIDSDHPEAEGNVGQCGVTLCTVEDYDDLIQGWDLNTTNFSLITGDNCLPALALITAAA
CCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHH
IRRGEEPGVLHGNSMNWYPRTAVQDIPSWEPKNGYLLMTDLIRYCSNNIPAWNTANLFMY
HHCCCCCCEEECCCCCCCCCHHHHCCCCCCCCCCEEEHHHHHHHHCCCCCCCCCCCEEEE
GLSEAGATPVQELAYGLAWGKSIIDACKERGLEPDSFVGRMGFQIGIQMNFFEEVAKLRA
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEHHHHHHHHHHHH
HRRMWAKITRDAGCTKPQCMHARVHVHTSGNVLTAQQPLNNTARITMQLMAAVMGGVQSI
HHHHHHHHHHCCCCCCCCEEEEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHH
HSCSYDETIGIPTELSHRTALRSQQIMMWESGLPDVADPLAGSYYIEALTDQMEQAAWKI
HCCCCCCCCCCCCCHHHHHHHHHHHEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHHHH
YNELEEQGGYRQGLADGSIKRAIDNSAYATKVKIKSGKQPVVGLNKYVMDRKDNYEPFKV
HHHHHHCCCHHCCCCCCHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHCCCCCCCCEEE
DTALEVKALERLKAFKAKRNQGDVDKALATVKNACLELRDGQGELMPVLVEAALKGATNG
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCH
EMCQVMRDVFGWVVTE
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8099072 [H]