BacMap

Definition	Geobacter metallireducens GS-15 chromosome, complete genome.
Accession	NC_007517
Length	3,997,420

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The map label for this gene is paaF [C]

Identifier: 78223412

GI number: 78223412

Start: 2497643

End: 2498440

Strand: Reverse

Name: paaF [C]

Synonym: Gmet_2207

Alternate gene names: 78223412

Gene position: 2498440-2497643 (Counterclockwise)

Preceding gene: 78223413

Following gene: 78223411

Centisome position: 62.5

GC content: 53.76

Gene sequence:

>798_bases
ATGTCTTTTGAGACTATCATTCTGGACAAGAAAGATGGCATTGCCACCATAACGTTTAACCGTCCCAAGGTATTCAACGC
CTACAGCGAGCAGATGTCTCAGGAGTTGAAGGCTGCCGTGGCGGATGTCGGATCCGATACTTCACTGCGTGTTCTCGTTC
TTAAAGGGAGTGGTGAAAACTTCCTCGCCGGCGCGGACATAAACATGCTCAATTCCTGGTCAAAGATCTCTGCGGAGCAG
GGCTGGGAAAAGGTTAAGGAGATCCTTGACCATCATTTCAGCCCAACGTCGCTGGAAAAAATTCCTCTGCCGGTCATTGC
GGCAGTAGATGGCATGGCTTGGGGGATGGGGAGCGAGATAGCGCTGGGGTGCGATTTCCGGATATGTACGACGCGGGCGT
CATTTGCCCAGCCTGAAATCAACCTTGGGATCATCACCGGCGGAGGGGCGAGCCAGCGGCTTCCCAGGATCGTCGGTAAG
GCAAAGGCGATGGAGATGATCCTGACCGGAAAGCCGATTAATGCGGCCGATGCCTGCAAATGGGGGTTGGTGAATGAGGT
TGTCGAGCCTGAAGGACTCGATGCTGCGGTTGCACGGCTTGCCAAGGCGATCATGGGGAAAAGCCCCCTGATGGTTAAAT
GGGCGAAGGATTGTGTCAACCTGGTGCTGGACCACGATCTTTTATCCGGCATTGACAAGGAGTTGACCCAGTTTGCCAAG
ACCTTTGCAACGCAGGATTCAAAGGAAGGGACCGCCGCATTTCTGGAAAAACGGAAGGCGAATTTCACGGGAGCATAA

Upstream 100 bases:

>100_bases
AGAAACCGTTGCCGAATACATCAAGGAAAATGTGGGCAAGGTCAGCTTGCGCTAGTGCCGTAGCAGCATAAACAAAACAA
CTGGTCGAAAGGTGAATCCC

Downstream 100 bases:

>100_bases
CGTCTCGGTGTGCATGAAGAGCTGTCGGGACTGTTCGGAAAAAAGCAACAATTCTATTTAAGGGAGAGCATATGAGTCTG
GACATATTCAATCTGGAGGG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MSFETIILDKKDGIATITFNRPKVFNAYSEQMSQELKAAVADVGSDTSLRVLVLKGSGENFLAGADINMLNSWSKISAEQ
GWEKVKEILDHHFSPTSLEKIPLPVIAAVDGMAWGMGSEIALGCDFRICTTRASFAQPEINLGIITGGGASQRLPRIVGK
AKAMEMILTGKPINAADACKWGLVNEVVEPEGLDAAVARLAKAIMGKSPLMVKWAKDCVNLVLDHDLLSGIDKELTQFAK
TFATQDSKEGTAAFLEKRKANFTGA

Sequences:

>Translated_265_residues
MSFETIILDKKDGIATITFNRPKVFNAYSEQMSQELKAAVADVGSDTSLRVLVLKGSGENFLAGADINMLNSWSKISAEQ
GWEKVKEILDHHFSPTSLEKIPLPVIAAVDGMAWGMGSEIALGCDFRICTTRASFAQPEINLGIITGGGASQRLPRIVGK
AKAMEMILTGKPINAADACKWGLVNEVVEPEGLDAAVARLAKAIMGKSPLMVKWAKDCVNLVLDHDLLSGIDKELTQFAK
TFATQDSKEGTAAFLEKRKANFTGA
>Mature_264_residues
SFETIILDKKDGIATITFNRPKVFNAYSEQMSQELKAAVADVGSDTSLRVLVLKGSGENFLAGADINMLNSWSKISAEQG
WEKVKEILDHHFSPTSLEKIPLPVIAAVDGMAWGMGSEIALGCDFRICTTRASFAQPEINLGIITGGGASQRLPRIVGKA
KAMEMILTGKPINAADACKWGLVNEVVEPEGLDAAVARLAKAIMGKSPLMVKWAKDCVNLVLDHDLLSGIDKELTQFAKT
FATQDSKEGTAAFLEKRKANFTGA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=264, Percent_Identity=40.530303030303, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI4502327, Length=262, Percent_Identity=32.0610687022901, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI31542718, Length=255, Percent_Identity=30.5882352941176, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI20127408, Length=187, Percent_Identity=34.7593582887701, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI45643119, Length=219, Percent_Identity=32.4200913242009, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI260275230, Length=219, Percent_Identity=32.4200913242009, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI260274832, Length=219, Percent_Identity=32.4200913242009, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI68989263, Length=193, Percent_Identity=30.5699481865285, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI150378541, Length=258, Percent_Identity=30.6201550387597, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI70995211, Length=268, Percent_Identity=27.6119402985075, Blast_Score=91, Evalue=7e-19,
Organism=Homo sapiens, GI221136756, Length=265, Percent_Identity=23.3962264150943, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI221136753, Length=265, Percent_Identity=23.3962264150943, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI213417737, Length=220, Percent_Identity=28.6363636363636, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI157694516, Length=220, Percent_Identity=28.6363636363636, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI221307494, Length=265, Percent_Identity=23.3962264150943, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI37594469, Length=193, Percent_Identity=26.9430051813472, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI37594471, Length=193, Percent_Identity=26.9430051813472, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI116235487, Length=260, Percent_Identity=24.2307692307692, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI25453481, Length=266, Percent_Identity=22.1804511278195, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI51538573, Length=266, Percent_Identity=22.1804511278195, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI4757966, Length=248, Percent_Identity=22.5806451612903, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI51538581, Length=248, Percent_Identity=22.5806451612903, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI4757968, Length=267, Percent_Identity=21.7228464419476, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI49355787, Length=267, Percent_Identity=21.7228464419476, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI261878539, Length=93, Percent_Identity=37.6344086021505, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1787659, Length=265, Percent_Identity=34.7169811320755, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI221142681, Length=274, Percent_Identity=32.4817518248175, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1788682, Length=213, Percent_Identity=36.150234741784, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1788597, Length=265, Percent_Identity=31.3207547169811, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1787660, Length=264, Percent_Identity=31.4393939393939, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI87082183, Length=270, Percent_Identity=28.5185185185185, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790281, Length=208, Percent_Identity=36.0576923076923, Blast_Score=107, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17554946, Length=260, Percent_Identity=36.9230769230769, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI25145438, Length=254, Percent_Identity=38.9763779527559, Blast_Score=156, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI17540714, Length=232, Percent_Identity=34.9137931034483, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17560910, Length=262, Percent_Identity=28.6259541984733, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17534483, Length=253, Percent_Identity=28.4584980237154, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17536985, Length=274, Percent_Identity=24.0875912408759, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17558304, Length=191, Percent_Identity=30.3664921465969, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25144276, Length=219, Percent_Identity=30.5936073059361, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17508951, Length=219, Percent_Identity=30.5936073059361, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17508953, Length=219, Percent_Identity=30.5936073059361, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17535521, Length=245, Percent_Identity=28.9795918367347, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=259, Percent_Identity=27.027027027027, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI25144157, Length=208, Percent_Identity=27.8846153846154, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25144160, Length=208, Percent_Identity=27.8846153846154, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=258, Percent_Identity=25.968992248062, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=204, Percent_Identity=27.4509803921569, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI20129971, Length=264, Percent_Identity=38.6363636363636, Blast_Score=168, Evalue=4e-42,
Organism=Drosophila melanogaster, GI24653477, Length=264, Percent_Identity=38.6363636363636, Blast_Score=168, Evalue=4e-42,
Organism=Drosophila melanogaster, GI24653139, Length=259, Percent_Identity=38.2239382239382, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI19920382, Length=265, Percent_Identity=32.0754716981132, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=27.2030651340996, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24654903, Length=197, Percent_Identity=26.9035532994924, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI21357171, Length=192, Percent_Identity=31.25, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24650670, Length=186, Percent_Identity=30.1075268817204, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19921000, Length=187, Percent_Identity=31.0160427807487, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583077, Length=187, Percent_Identity=31.0160427807487, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583079, Length=187, Percent_Identity=31.0160427807487, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28571730, Length=176, Percent_Identity=28.9772727272727, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28571729, Length=176, Percent_Identity=28.9772727272727, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28516; Mature: 28385

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFETIILDKKDGIATITFNRPKVFNAYSEQMSQELKAAVADVGSDTSLRVLVLKGSGEN
CCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
FLAGADINMLNSWSKISAEQGWEKVKEILDHHFSPTSLEKIPLPVIAAVDGMAWGMGSEI
EEECCCHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCHHCCCCCCE
ALGCDFRICTTRASFAQPEINLGIITGGGASQRLPRIVGKAKAMEMILTGKPINAADACK
EEECCEEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHEEECCCCCHHHHHH
WGLVNEVVEPEGLDAAVARLAKAIMGKSPLMVKWAKDCVNLVLDHDLLSGIDKELTQFAK
HHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFATQDSKEGTAAFLEKRKANFTGA
HHHCCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SFETIILDKKDGIATITFNRPKVFNAYSEQMSQELKAAVADVGSDTSLRVLVLKGSGEN
CCEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
FLAGADINMLNSWSKISAEQGWEKVKEILDHHFSPTSLEKIPLPVIAAVDGMAWGMGSEI
EEECCCHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCHHCCCCCCE
ALGCDFRICTTRASFAQPEINLGIITGGGASQRLPRIVGKAKAMEMILTGKPINAADACK
EEECCEEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHEEECCCCCHHHHHH
WGLVNEVVEPEGLDAAVARLAKAIMGKSPLMVKWAKDCVNLVLDHDLLSGIDKELTQFAK
HHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFATQDSKEGTAAFLEKRKANFTGA
HHHCCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA