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The map label for this gene is fadJ [H]

Identifier: 78223401

GI number: 78223401

Start: 2483530

End: 2484369

Strand: Reverse

Name: fadJ [H]

Synonym: Gmet_2196

Alternate gene names: 78223401

Gene position: 2484369-2483530 (Counterclockwise)

Preceding gene: 78223402

Following gene: 78223400

Centisome position: 62.15

GC content: 56.79

Gene sequence:

>840_bases
ATGAGCGCATACAGATATGAAAGGGATGGAGAGGGTATCGTAACCGTTACCATCAATCCCGCAGGCCAGCCGGCCAGCAT
CATGGATGAAGGAACGGCGGGAGAGCTTCTCGATCGCCTTGAACGCGATACCATCAGTGGTGTCATTATCACCATTGATC
AGGCATCACCCGCTACCGCCTGCACTGTCAGCGAAAACAGCCTTTCGGCACGTCAGCAGGTTTTTAACCGCCTCAGTGCT
TACAAAAGCATGCTGCGGCGTCTTGAAACTCTTGGCAAACCGGTCGTTGCAGCCATCAATGGTCCGACTCAGGGAAGAGA
TTACGAACTTTGCCTGGCCGCCCATCACCGCATAGCGGTCAACGATCTTGCCATCGCGATCGGCCTGCCGGACGTAGCGG
CAGGTCTGATTCCCTGCAACGGTGGAGTAGCGCGCTTGGTCCGCCTGATTGGGCTCGAAAAGGGGTTGCCGCTCCTCATG
GCAGGAACAAAACTTGCCCCGGAAAAGGCGAAAGAGACTGGTCTGATTGACCAACTAGCTGCCAGCAGTGGCGAACTCCA
ATCCATGGCACGAGACTGGATCGCTGCCAATCCCGCTGCCGGTCAGCCCTGGGACCAGAAAGGATATAAGATGCCGGGGG
GGACACCTTCCAGCCCCAATATAGCGCAACAGATCTTTGTTGCACCGGCCATCCTGCGGAAAAAATGCGCTGACGGGCAA
CCGGCACAGGAATCGATACTGGCGGTGGCGGTCGAAGGCGCCCAAGTCGACCTGGAATCGGCTCTGCGCATCGAATCCCG
CTATTTTACGGATCTGCTGTTTCGAACAGCTGCCGGTTAA

Upstream 100 bases:

>100_bases
GCCTGTGCACCCTCTGCGTCGGTGGCGGAATCGGGATAGCGACCATTGTCGAACGGCTGTGACAGCGGCACACAATCAAA
CAATAAAAGGTGGGACATAT

Downstream 100 bases:

>100_bases
CGCGGAATGCCGGTGTGGCAAAAACTGAAATCAATCTGGATTCCCTGAGGAGAGAACCATGACCGAAAAATTCGAAACGA
TTATCTTCGAAAAGCGCGGA

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase; 3-hydroxyacyl-CoA dehydrogenase [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MSAYRYERDGEGIVTVTINPAGQPASIMDEGTAGELLDRLERDTISGVIITIDQASPATACTVSENSLSARQQVFNRLSA
YKSMLRRLETLGKPVVAAINGPTQGRDYELCLAAHHRIAVNDLAIAIGLPDVAAGLIPCNGGVARLVRLIGLEKGLPLLM
AGTKLAPEKAKETGLIDQLAASSGELQSMARDWIAANPAAGQPWDQKGYKMPGGTPSSPNIAQQIFVAPAILRKKCADGQ
PAQESILAVAVEGAQVDLESALRIESRYFTDLLFRTAAG

Sequences:

>Translated_279_residues
MSAYRYERDGEGIVTVTINPAGQPASIMDEGTAGELLDRLERDTISGVIITIDQASPATACTVSENSLSARQQVFNRLSA
YKSMLRRLETLGKPVVAAINGPTQGRDYELCLAAHHRIAVNDLAIAIGLPDVAAGLIPCNGGVARLVRLIGLEKGLPLLM
AGTKLAPEKAKETGLIDQLAASSGELQSMARDWIAANPAAGQPWDQKGYKMPGGTPSSPNIAQQIFVAPAILRKKCADGQ
PAQESILAVAVEGAQVDLESALRIESRYFTDLLFRTAAG
>Mature_278_residues
SAYRYERDGEGIVTVTINPAGQPASIMDEGTAGELLDRLERDTISGVIITIDQASPATACTVSENSLSARQQVFNRLSAY
KSMLRRLETLGKPVVAAINGPTQGRDYELCLAAHHRIAVNDLAIAIGLPDVAAGLIPCNGGVARLVRLIGLEKGLPLLMA
GTKLAPEKAKETGLIDQLAASSGELQSMARDWIAANPAAGQPWDQKGYKMPGGTPSSPNIAQQIFVAPAILRKKCADGQP
AQESILAVAVEGAQVDLESALRIESRYFTDLLFRTAAG

Specific function: Catalyzes the formation of an hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the central section; belongs to the 3-hydroxyacyl- CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI20127408, Length=206, Percent_Identity=28.1553398058252, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1788682, Length=102, Percent_Identity=36.2745098039216, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1790281, Length=249, Percent_Identity=25.3012048192771, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17558304, Length=190, Percent_Identity=28.9473684210526, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17508953, Length=201, Percent_Identity=29.3532338308458, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25144276, Length=201, Percent_Identity=29.3532338308458, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17508951, Length=201, Percent_Identity=29.3532338308458, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006180
- InterPro:   IPR006176
- InterPro:   IPR006108
- InterPro:   IPR008927
- InterPro:   IPR001753
- InterPro:   IPR013328
- InterPro:   IPR018376
- InterPro:   IPR012802
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00725 3HCDH; PF02737 3HCDH_N; PF00378 ECH [H]

EC number: =4.2.1.17; =5.1.2.3; =1.1.1.35 [H]

Molecular weight: Translated: 29504; Mature: 29373

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAYRYERDGEGIVTVTINPAGQPASIMDEGTAGELLDRLERDTISGVIITIDQASPATA
CCCCCCCCCCCEEEEEEECCCCCCHHHHCCCCHHHHHHHHHHHCCCEEEEEEECCCCCEE
CTVSENSLSARQQVFNRLSAYKSMLRRLETLGKPVVAAINGPTQGRDYELCLAAHHRIAV
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEHHCCEEEE
NDLAIAIGLPDVAAGLIPCNGGVARLVRLIGLEKGLPLLMAGTKLAPEKAKETGLIDQLA
EEEEEEEECCHHHHCCEECCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHCCHHHHHH
ASSGELQSMARDWIAANPAAGQPWDQKGYKMPGGTPSSPNIAQQIFVAPAILRKKCADGQ
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
PAQESILAVAVEGAQVDLESALRIESRYFTDLLFRTAAG
CHHHCEEEEEECCCEECHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAYRYERDGEGIVTVTINPAGQPASIMDEGTAGELLDRLERDTISGVIITIDQASPATA
CCCCCCCCCCEEEEEEECCCCCCHHHHCCCCHHHHHHHHHHHCCCEEEEEEECCCCCEE
CTVSENSLSARQQVFNRLSAYKSMLRRLETLGKPVVAAINGPTQGRDYELCLAAHHRIAV
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEHHCCEEEE
NDLAIAIGLPDVAAGLIPCNGGVARLVRLIGLEKGLPLLMAGTKLAPEKAKETGLIDQLA
EEEEEEEECCHHHHCCEECCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHCCHHHHHH
ASSGELQSMARDWIAANPAAGQPWDQKGYKMPGGTPSSPNIAQQIFVAPAILRKKCADGQ
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
PAQESILAVAVEGAQVDLESALRIESRYFTDLLFRTAAG
CHHHCEEEEEECCCEECHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA