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The map label for this gene is bclA [H]

Identifier: 78223350

GI number: 78223350

Start: 2410719

End: 2412290

Strand: Direct

Name: bclA [H]

Synonym: Gmet_2143

Alternate gene names: 78223350

Gene position: 2410719-2412290 (Clockwise)

Preceding gene: 78223347

Following gene: 78223351

Centisome position: 60.31

GC content: 61.32

Gene sequence:

>1572_bases
ATGCCCTACAATCTCAACCTGCCCGAAACCTTCAACGCCGCCGATTACTTCATCGACCGCAACATCCGCGAGGGGAGAGG
CGACAAGCTCGCCGTTCTCTGCGAGGAGCGCTCCCTCACCTACCGGCAGCTGCAGGAAGGGGTCAACCGCTTCGGAAACG
CCCTGAAATCCCTGGATGTGCGCATGGAAGAGCGCGTCGCCCTCCTTCTGCTGGATACGGAGGTCTACCCCCAGGCATTT
TTCGGAGCCATCAAGATCGGGGCGGTTCCCATCTGCCTCAACACCATGAACCGTCCCCAGGATTTCCAGTTCTACCTCAA
CGACAGCCGGGCGCGGGTCCTGGTTGTCGATGCGCTGCTCCTTGACCAGATCGAGCCCATTCGCCGCAACCTGCAGTTCC
TGAAGCATGTCATCGTGGCCAACGGCTCCGCCCCGGCAGGCGATCTGAGCCTGGCGGAACTGTGCGCCCCCCAGCCCACG
GAGCTTGAAACCGCCCCCACCTGCCGCGACGACGCCTGCTTCTGGCTCTACAGCTCCGGCTCCACCGGCTCACCCAAGGG
GACGGTCCATCTCCAGCACGACATGGTCTATTCGGCCAAGACCTACGGGGCAAAAGTGCTCGACATCAAGGAAAACGACG
TATTTTTCTCGGCCGCGAAACTGTTCTTTGCCTACGGCCTGGGGAACGGCATCTACTTCCCTTTCTGTGTCGGGGCAACC
GCCGTGTACCTGCCGCTGCGGCCGACCCCGGCCAATGTTTATGAAACGGTGCGCCGCCACCGTCCGACCCTGTTTTTCGG
CGTTCCCACCCTCTACGGCCAGATGCTTGAGGAAGAAGGGAGCATGAACGGCGTCAGGCTGTGCGTCTCCGCGGGCGAGG
CCCTGCCGGCAGCCTACATCCACCGCTGGAAAGCACGCTTCCAGCTGGACATTCTCGACGGGATCGGCTCCACCGAGATG
GCCCACATCTTCATCTCCAACAGGCCCGGCGAAATCGTCGCCGGCAGCTCCGGGAGGGTCGTCCCCGGCTACGAGGCCCG
CATCGTGGATGAAAACATGCACGATCTCCCGGCCGGCGAGATCGGCACGCTCCTGGTCAAGGGTGACAGCGCCACGGCCC
TCTACTGGAACAAGCATGAGAAAACCAGGCAGACCATCATGGGGCACTGGATAAATACCGGCGACAAATATGTCTGCGAT
GAAAACGGCTATTTCCACTGCGCCGGCCGCTCCGACGACATGCTCAAGGTGGGGGGGATCTGGGTGTCGCCCAACGAGGT
TGAGTCTTGCCTGATCGGACACCCCGCCATCCTGGAGTGTGCCGTCATCGGCGCCCCTGACGAGGACAACCTGATCAAGC
CCATGGCCTTCGTGGTCCTCAATTCCCAGCATCAGCCATCGCCGGAGATGGAGAACGAGTTGAAGGAGTATGTGAAGACC
ACCCTGGCGATGTACAAGTACCCGCGCTGGATACGGTTCATCGATGAACTCCCCAAGACCGCCACCGGCAAGATCAAACG
CTTCGAACTGCGCACCATGATCCAGAAGGAAGAGTTGCAGCACGTGGCCTGA

Upstream 100 bases:

>100_bases
GTTGTAAATTTGACGGTTCGTATCATTTCACTTGGTCCAGTATCTGCAAAACACTGTTTATCAAATTAACACCTTAACCA
CAAGCTGCCGGAGGTCCACA

Downstream 100 bases:

>100_bases
ATCAGGGGCCTGATCGTCACGAACACACTCGATGCACTACCTGCCAAGGAGGTACGGGATGGAGAAATACGCAGTCAGAA
GAATGTTCAAGGGTCAACTG

Product: benzoate-CoA ligase family

Products: NA

Alternate protein names: Benzoyl-CoA synthetase [H]

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MPYNLNLPETFNAADYFIDRNIREGRGDKLAVLCEERSLTYRQLQEGVNRFGNALKSLDVRMEERVALLLLDTEVYPQAF
FGAIKIGAVPICLNTMNRPQDFQFYLNDSRARVLVVDALLLDQIEPIRRNLQFLKHVIVANGSAPAGDLSLAELCAPQPT
ELETAPTCRDDACFWLYSSGSTGSPKGTVHLQHDMVYSAKTYGAKVLDIKENDVFFSAAKLFFAYGLGNGIYFPFCVGAT
AVYLPLRPTPANVYETVRRHRPTLFFGVPTLYGQMLEEEGSMNGVRLCVSAGEALPAAYIHRWKARFQLDILDGIGSTEM
AHIFISNRPGEIVAGSSGRVVPGYEARIVDENMHDLPAGEIGTLLVKGDSATALYWNKHEKTRQTIMGHWINTGDKYVCD
ENGYFHCAGRSDDMLKVGGIWVSPNEVESCLIGHPAILECAVIGAPDEDNLIKPMAFVVLNSQHQPSPEMENELKEYVKT
TLAMYKYPRWIRFIDELPKTATGKIKRFELRTMIQKEELQHVA

Sequences:

>Translated_523_residues
MPYNLNLPETFNAADYFIDRNIREGRGDKLAVLCEERSLTYRQLQEGVNRFGNALKSLDVRMEERVALLLLDTEVYPQAF
FGAIKIGAVPICLNTMNRPQDFQFYLNDSRARVLVVDALLLDQIEPIRRNLQFLKHVIVANGSAPAGDLSLAELCAPQPT
ELETAPTCRDDACFWLYSSGSTGSPKGTVHLQHDMVYSAKTYGAKVLDIKENDVFFSAAKLFFAYGLGNGIYFPFCVGAT
AVYLPLRPTPANVYETVRRHRPTLFFGVPTLYGQMLEEEGSMNGVRLCVSAGEALPAAYIHRWKARFQLDILDGIGSTEM
AHIFISNRPGEIVAGSSGRVVPGYEARIVDENMHDLPAGEIGTLLVKGDSATALYWNKHEKTRQTIMGHWINTGDKYVCD
ENGYFHCAGRSDDMLKVGGIWVSPNEVESCLIGHPAILECAVIGAPDEDNLIKPMAFVVLNSQHQPSPEMENELKEYVKT
TLAMYKYPRWIRFIDELPKTATGKIKRFELRTMIQKEELQHVA
>Mature_522_residues
PYNLNLPETFNAADYFIDRNIREGRGDKLAVLCEERSLTYRQLQEGVNRFGNALKSLDVRMEERVALLLLDTEVYPQAFF
GAIKIGAVPICLNTMNRPQDFQFYLNDSRARVLVVDALLLDQIEPIRRNLQFLKHVIVANGSAPAGDLSLAELCAPQPTE
LETAPTCRDDACFWLYSSGSTGSPKGTVHLQHDMVYSAKTYGAKVLDIKENDVFFSAAKLFFAYGLGNGIYFPFCVGATA
VYLPLRPTPANVYETVRRHRPTLFFGVPTLYGQMLEEEGSMNGVRLCVSAGEALPAAYIHRWKARFQLDILDGIGSTEMA
HIFISNRPGEIVAGSSGRVVPGYEARIVDENMHDLPAGEIGTLLVKGDSATALYWNKHEKTRQTIMGHWINTGDKYVCDE
NGYFHCAGRSDDMLKVGGIWVSPNEVESCLIGHPAILECAVIGAPDEDNLIKPMAFVVLNSQHQPSPEMENELKEYVKTT
LAMYKYPRWIRFIDELPKTATGKIKRFELRTMIQKEELQHVA

Specific function: Catalyzes the ligation of benzoate and CoA to form benzoyl-CoA at the expense of ATP. The enzyme also ligates 2- aminobenzoate and CoA. The enzyme shows activity toward a number of benzoate derivates [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. Benzoate-CoA ligase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=531, Percent_Identity=28.060263653484, Blast_Score=209, Evalue=7e-54,
Organism=Homo sapiens, GI122937307, Length=529, Percent_Identity=28.1663516068053, Blast_Score=192, Evalue=9e-49,
Organism=Homo sapiens, GI38505220, Length=491, Percent_Identity=28.9205702647658, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI58082049, Length=490, Percent_Identity=27.3469387755102, Blast_Score=187, Evalue=3e-47,
Organism=Homo sapiens, GI115511026, Length=531, Percent_Identity=27.683615819209, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI157311624, Length=490, Percent_Identity=26.530612244898, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI157311622, Length=490, Percent_Identity=26.530612244898, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI28416953, Length=558, Percent_Identity=27.2401433691756, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI187761345, Length=533, Percent_Identity=27.9549718574109, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI187761343, Length=533, Percent_Identity=27.9549718574109, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI8923896, Length=577, Percent_Identity=27.3830155979203, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI156151445, Length=528, Percent_Identity=24.8106060606061, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI13375727, Length=551, Percent_Identity=23.5934664246824, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI253970489, Length=391, Percent_Identity=30.4347826086957, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI42544134, Length=377, Percent_Identity=22.5464190981432, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI195947378, Length=410, Percent_Identity=22.9268292682927, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI13325057, Length=553, Percent_Identity=27.3056057866184, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI38524616, Length=515, Percent_Identity=23.8834951456311, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI13236579, Length=483, Percent_Identity=25.2587991718427, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI62865631, Length=540, Percent_Identity=23.1481481481481, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI13325055, Length=540, Percent_Identity=23.1481481481481, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1790505, Length=548, Percent_Identity=28.2846715328467, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI1788107, Length=524, Percent_Identity=29.0076335877863, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI145693145, Length=524, Percent_Identity=28.8167938931298, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI221142682, Length=508, Percent_Identity=28.740157480315, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1789201, Length=506, Percent_Identity=25.296442687747, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1786810, Length=506, Percent_Identity=22.5296442687747, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1786529, Length=536, Percent_Identity=23.5074626865672, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788595, Length=510, Percent_Identity=23.5294117647059, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1786801, Length=440, Percent_Identity=24.3181818181818, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17558820, Length=513, Percent_Identity=24.9512670565302, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI71994690, Length=372, Percent_Identity=29.8387096774194, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI71994694, Length=372, Percent_Identity=29.8387096774194, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI71994703, Length=372, Percent_Identity=29.8387096774194, Blast_Score=145, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI32563687, Length=514, Percent_Identity=24.5136186770428, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17559526, Length=526, Percent_Identity=26.425855513308, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71982997, Length=569, Percent_Identity=25.3075571177504, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71983001, Length=569, Percent_Identity=25.3075571177504, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17531443, Length=453, Percent_Identity=24.9448123620309, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17557194, Length=519, Percent_Identity=25.2408477842004, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17560308, Length=527, Percent_Identity=24.4781783681214, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17538037, Length=515, Percent_Identity=23.495145631068, Blast_Score=112, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17560140, Length=492, Percent_Identity=25.8130081300813, Blast_Score=102, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI71985884, Length=352, Percent_Identity=24.7159090909091, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71996755, Length=508, Percent_Identity=21.8503937007874, Blast_Score=90, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6319264, Length=535, Percent_Identity=26.7289719626168, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6319699, Length=516, Percent_Identity=26.1627906976744, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6323182, Length=538, Percent_Identity=25.6505576208178, Blast_Score=136, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24581924, Length=529, Percent_Identity=24.952741020794, Blast_Score=160, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24667955, Length=559, Percent_Identity=26.654740608229, Blast_Score=160, Evalue=3e-39,
Organism=Drosophila melanogaster, GI62472339, Length=532, Percent_Identity=26.1278195488722, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI18859661, Length=507, Percent_Identity=27.6134122287968, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI21355181, Length=519, Percent_Identity=24.6628131021195, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24667959, Length=363, Percent_Identity=28.099173553719, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24656500, Length=505, Percent_Identity=23.5643564356436, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21355225, Length=576, Percent_Identity=22.0486111111111, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24648260, Length=475, Percent_Identity=24.4210526315789, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19922652, Length=522, Percent_Identity=22.7969348659004, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21356441, Length=495, Percent_Identity=23.2323232323232, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356947, Length=426, Percent_Identity=24.6478873239437, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI28573631, Length=549, Percent_Identity=24.2258652094718, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20130357, Length=513, Percent_Identity=22.2222222222222, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI20130353, Length=549, Percent_Identity=24.2258652094718, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24648253, Length=495, Percent_Identity=23.2323232323232, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24648255, Length=495, Percent_Identity=23.030303030303, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI281365686, Length=419, Percent_Identity=21.2410501193317, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24653035, Length=201, Percent_Identity=30.8457711442786, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011957 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.25 [H]

Molecular weight: Translated: 58544; Mature: 58413

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYNLNLPETFNAADYFIDRNIREGRGDKLAVLCEERSLTYRQLQEGVNRFGNALKSLDV
CCCCCCCCCCCCCHHHHEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCC
RMEERVALLLLDTEVYPQAFFGAIKIGAVPICLNTMNRPQDFQFYLNDSRARVLVVDALL
CCCCCEEEEEEECCCCHHHHHCCCEECCEEEEECCCCCCCCEEEEECCCCCEEEEEEHHH
LDQIEPIRRNLQFLKHVIVANGSAPAGDLSLAELCAPQPTELETAPTCRDDACFWLYSSG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEEECC
STGSPKGTVHLQHDMVYSAKTYGAKVLDIKENDVFFSAAKLFFAYGLGNGIYFPFCVGAT
CCCCCCCEEEEEECEEEEHHHCCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEHEECCCE
AVYLPLRPTPANVYETVRRHRPTLFFGVPTLYGQMLEEEGSMNGVRLCVSAGEALPAAYI
EEEEECCCCCHHHHHHHHHCCCEEEEECHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
HRWKARFQLDILDGIGSTEMAHIFISNRPGEIVAGSSGRVVPGYEARIVDENMHDLPAGE
HHHHHEEEEEEECCCCCCEEEEEEEECCCCCEEECCCCCCCCCCCEEEECCCCCCCCCCC
IGTLLVKGDSATALYWNKHEKTRQTIMGHWINTGDKYVCDENGYFHCAGRSDDMLKVGGI
EEEEEEECCCCEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCEEEECCCCCCEEEECCE
WVSPNEVESCLIGHPAILECAVIGAPDEDNLIKPMAFVVLNSQHQPSPEMENELKEYVKT
EECHHHHHHHHCCCCHHEEEEEECCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
TLAMYKYPRWIRFIDELPKTATGKIKRFELRTMIQKEELQHVA
HHHHHHCHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PYNLNLPETFNAADYFIDRNIREGRGDKLAVLCEERSLTYRQLQEGVNRFGNALKSLDV
CCCCCCCCCCCCHHHHEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCC
RMEERVALLLLDTEVYPQAFFGAIKIGAVPICLNTMNRPQDFQFYLNDSRARVLVVDALL
CCCCCEEEEEEECCCCHHHHHCCCEECCEEEEECCCCCCCCEEEEECCCCCEEEEEEHHH
LDQIEPIRRNLQFLKHVIVANGSAPAGDLSLAELCAPQPTELETAPTCRDDACFWLYSSG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEEECC
STGSPKGTVHLQHDMVYSAKTYGAKVLDIKENDVFFSAAKLFFAYGLGNGIYFPFCVGAT
CCCCCCCEEEEEECEEEEHHHCCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEHEECCCE
AVYLPLRPTPANVYETVRRHRPTLFFGVPTLYGQMLEEEGSMNGVRLCVSAGEALPAAYI
EEEEECCCCCHHHHHHHHHCCCEEEEECHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
HRWKARFQLDILDGIGSTEMAHIFISNRPGEIVAGSSGRVVPGYEARIVDENMHDLPAGE
HHHHHEEEEEEECCCCCCEEEEEEEECCCCCEEECCCCCCCCCCCEEEECCCCCCCCCCC
IGTLLVKGDSATALYWNKHEKTRQTIMGHWINTGDKYVCDENGYFHCAGRSDDMLKVGGI
EEEEEEECCCCEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCEEEECCCCCCEEEECCE
WVSPNEVESCLIGHPAILECAVIGAPDEDNLIKPMAFVVLNSQHQPSPEMENELKEYVKT
EECHHHHHHHHCCCCHHEEEEEECCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
TLAMYKYPRWIRFIDELPKTATGKIKRFELRTMIQKEELQHVA
HHHHHHCHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12897012 [H]