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The map label for this gene is pucB [H]

Identifier: 78223347

GI number: 78223347

Start: 2406812

End: 2407420

Strand: Direct

Name: pucB [H]

Synonym: Gmet_2140

Alternate gene names: 78223347

Gene position: 2406812-2407420 (Clockwise)

Preceding gene: 78223346

Following gene: 78223350

Centisome position: 60.21

GC content: 65.19

Gene sequence:

>609_bases
ATGGAGGCCCGTCCCAGGGTTGCCGGGATTGTCCTCGCCGCCGGCAAGAGCACCCGCATGGGGCGGCCAAAGCAGCTCCT
CCCCTTCCGGGGCCGGACCGTTCTGGAATGTGTCGTCGACAATGCCCTTGCCTCACGCCTCCACCGCGTTGTCGTGGTGC
TCGGCCACGAGGCGGAGGCCGTCATGCCGCTTCTTGGGCACCGGTGCGTCACAACCGTCGTAAACCCCTTGTACGGTGAG
GGTCAGAGCTCGTCGCTGAAGGCGGGCCTGGGGGCGATGACCGAAGAGACCGATGCCGCCCTCTTTCTCCTGGGCGACCA
ACCCCTCGTCACCGCCGACACCATCAACCTTCTCATCGCCGCCTTCGCCGACTTCCCCTCCCCCATCGTCATCCCCACCT
TTGAAGGCCACAGGGGAAATCCGGTCCTCTTCAGCCGGGAGACCTTCCCCCGCATCGAGGCGTTGAGCGGAGATCGCGGC
GCCAGGGGGCTCTTCCGGGAGTATGGCGATGATATCCGCAGCATCGACGTGCGAACCCCGGCTATCCTCTTCGATCTGGA
TACGGAGGAGGATTACCTCCGCCTGTTGCGGGAATTCCCCGCGGTGTAA

Upstream 100 bases:

>100_bases
TCCAGCTCAAGGAGATCGCGCCTTCCGTTGCCGAAGCGGTCATCGTGGGGCAACTGCGGCAGGAGATCACCCTGCACCGG
GTCTATCCGAGGGGAGAGTA

Downstream 100 bases:

>100_bases
GCCCAACTCCCCGCTCATTGCCGCCGAACCCTCTTAAGCACCCCGGCCACACTTGTCAGTCACGACAAAAACCAGCACCG
CCATTGACAGCTCGCACCCC

Product: molybdenum cofactor biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MEARPRVAGIVLAAGKSTRMGRPKQLLPFRGRTVLECVVDNALASRLHRVVVVLGHEAEAVMPLLGHRCVTTVVNPLYGE
GQSSSLKAGLGAMTEETDAALFLLGDQPLVTADTINLLIAAFADFPSPIVIPTFEGHRGNPVLFSRETFPRIEALSGDRG
ARGLFREYGDDIRSIDVRTPAILFDLDTEEDYLRLLREFPAV

Sequences:

>Translated_202_residues
MEARPRVAGIVLAAGKSTRMGRPKQLLPFRGRTVLECVVDNALASRLHRVVVVLGHEAEAVMPLLGHRCVTTVVNPLYGE
GQSSSLKAGLGAMTEETDAALFLLGDQPLVTADTINLLIAAFADFPSPIVIPTFEGHRGNPVLFSRETFPRIEALSGDRG
ARGLFREYGDDIRSIDVRTPAILFDLDTEEDYLRLLREFPAV
>Mature_202_residues
MEARPRVAGIVLAAGKSTRMGRPKQLLPFRGRTVLECVVDNALASRLHRVVVVLGHEAEAVMPLLGHRCVTTVVNPLYGE
GQSSSLKAGLGAMTEETDAALFLLGDQPLVTADTINLLIAAFADFPSPIVIPTFEGHRGNPVLFSRETFPRIEALSGDRG
ARGLFREYGDDIRSIDVRTPAILFDLDTEEDYLRLLREFPAV

Specific function: Required for xanthine dehydrogenase activity. Could be involved in formation of the molybdenum cofactor required by xanthine dehydrogenase [H]

COG id: COG2068

COG function: function code R; Uncharacterized MobA-related protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789242, Length=185, Percent_Identity=26.4864864864865, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017615 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 22037; Mature: 22037

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEARPRVAGIVLAAGKSTRMGRPKQLLPFRGRTVLECVVDNALASRLHRVVVVLGHEAEA
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCHHH
VMPLLGHRCVTTVVNPLYGEGQSSSLKAGLGAMTEETDAALFLLGDQPLVTADTINLLIA
HHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCEEEEEECCCCEEHHHHHHHHHH
AFADFPSPIVIPTFEGHRGNPVLFSRETFPRIEALSGDRGARGLFREYGDDIRSIDVRTP
HHHCCCCCEEEECCCCCCCCEEEEECCCCCCCEECCCCCCCHHHHHHHCCHHEEEECCCC
AILFDLDTEEDYLRLLREFPAV
EEEEECCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MEARPRVAGIVLAAGKSTRMGRPKQLLPFRGRTVLECVVDNALASRLHRVVVVLGHEAEA
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCHHH
VMPLLGHRCVTTVVNPLYGEGQSSSLKAGLGAMTEETDAALFLLGDQPLVTADTINLLIA
HHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCEEEEEECCCCEEHHHHHHHHHH
AFADFPSPIVIPTFEGHRGNPVLFSRETFPRIEALSGDRGARGLFREYGDDIRSIDVRTP
HHHCCCCCEEEECCCCCCCCEEEEECCCCCCCEECCCCCCCHHHHHHHCCHHEEEECCCC
AILFDLDTEEDYLRLLREFPAV
EEEEECCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]