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The map label for this gene is gltD [H]

Identifier: 78223325

GI number: 78223325

Start: 2386317

End: 2388164

Strand: Direct

Name: gltD [H]

Synonym: Gmet_2118

Alternate gene names: 78223325

Gene position: 2386317-2388164 (Clockwise)

Preceding gene: 78223324

Following gene: 78223326

Centisome position: 59.7

GC content: 65.8

Gene sequence:

>1848_bases
ATGGCAAACCCAAGCGGCAAATATCAGGTGAAGATCCTGGGCATCGAGGACTATCAGGCCTTGACGGCATGTCAGAGCGC
CTGCCCCCTGGGAACTGATACCAAGCGGTACGTACGGGCCATCACCGAAGGAGATTACGAGAAGGCTTTTCTCATCGCCC
GGCAGACCAATCCCCTGGTGTCGGTCTGCAGCAGGGTCTGCACCGCCCCCTGCGAAAAGTCCTGCCGGAAAGGGGAGCAA
GGACACCCGGTTGATATCCGCGCCCTGAAGCGGTTCGCCTGCGACAAGCATGGCGTGACATCGCCCCGCGCCGTCGCCGG
GAGGCTTGAAGAGATCTCGCGCCAGAACGGAGGGGTGGGAGACCGGACCGGCAACCATATCCTGACCATGGCCGCCGTGC
AAAAGGCCGGCGCAGCGGCCACATCGCGAAAGCCTGCCCCCCGGGTGGCCATCATCGGGTCCGGCCCGACCGGCCTCTCG
GCAGCCCACGATCTCGCCCTGCTCGGCTACGGGGTGACCATCTTCGAGGCAGCCCCCTACGCGGGGGGGATGCTCCGCAC
CGGGATTCCGGCCTTCCGCCTCCCCAAAGACGTGCTCCAGCAGGAGATCGATGCGATCCTGAATCTCGGCGTGGAACTGA
AGCTGAACAGCCCCATCGGCGAAGACCTGACCCTGGCCGACCTCAAGGCGCAGGGGTACGAGTCGGTGTTCATCACCATC
GGCCTGCAAGATCCCCTGCGGATTCTGAACATCGAAGGGACCGACCTGGCGGGGATCTACAGCGGCGTCGAGTATGTGCG
GGATCACGAAAAGATCCGGCTCGGGAAACGCTGTCTGGTCATCGGAGGCGGCGGGGTGGCCATTGACTGCGCCCAGCACG
CCGTGCGGCAGGGGGCGGAGCAGGTGATGATCGCCTGCCTCGAGTCCTGGGAGACGATGCCCGCAAGCCTGACGGAACGG
GAAGACGCCCAGGAGGAGGGGATCGTGTTCCACCCTGCCCTCGGCCCGCGCCGCTTCATGGGCCACAACGGGCACGTCGC
CCGGGCCGAGTTCCTCCAGGTGAGCTCCGTCTTCGACAGCGAAGGACGATTCGCCCCCACTTTTGTCCCGGACAGCACCA
CCACCCTGGAAGTCGATTCGGTCATCCTCGCCGTGGGCCAGGCCTCCACTGCCCCTTCCCTGGCGGGACTCGCCGGGCTG
GACCTCACCCCCAACGGACTGATCAAGGCGGCAGAGGACATGTCCACCAACCTCCCCGGCGTTTTTGCCGGCGGCGACGT
GCGCTGGCGCTTCACCCGCAATGCCACGGACGCCATTGCCGACGGGCAGAAGGCGGCCCGCGCCATCCATGGTTATCTCA
GCGGCCATACGCCCCAGGTGATCCGGAAGGGGTTCATGCGGCCGGTGTCGCCGGACTTCGAGAATACCCGCTGCGACACC
ATCGCCCCGGTACCGATCCCCAAGCGTGATGCCGGCGAGCGGGTCCGGACCAAAGAGGAGATCACCATCGGCTTCGGAGA
GGCGGAGGCACGGGAGCAAGCCGCCCGGTGCCGGCGGTGCAACATCCAGACGGTGTTCGAGCGGTCCCGGTGCCTGCGGT
GCGGCACCTGCGTCGACACCTGCGTGAATGGCGCCCTGAGGATAGTCCGGCTGGCGGATATCCAGGGTGACGAAGATGTG
GCGAAGCTGACCGATGCCCTGAAGAAGAGCGCGCAGACCCGCAGCAAGGCAATGACCGCGATCATCAAGGACGAGAGCCG
CTGCGCCAGCTGCGGCATGTGCGCCCGACGCTGCCCCGGCGGAGCCATAACCATGGCCGAGTTCTACTGCCAAGAGGAAT
GGGAATAA

Upstream 100 bases:

>100_bases
CGGGCACCCCTGCCCCCATCCGGACCTCCCCCTGCTCCCGAATAAGTGAGTCCATGTCGTTCCAGCCCTTAAGAGAGCAC
AACGAACAGAGGAGAAGACG

Downstream 100 bases:

>100_bases
CCAACAACCTGAGTACGCGGAGACCAATCATGAGTGAATCAGCCTACAGCATTACAGAAGCCATCCGGCAACTTCCAAGA
AACATCAAGGCGTCCATCTC

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 615; Mature: 614

Protein sequence:

>615_residues
MANPSGKYQVKILGIEDYQALTACQSACPLGTDTKRYVRAITEGDYEKAFLIARQTNPLVSVCSRVCTAPCEKSCRKGEQ
GHPVDIRALKRFACDKHGVTSPRAVAGRLEEISRQNGGVGDRTGNHILTMAAVQKAGAAATSRKPAPRVAIIGSGPTGLS
AAHDLALLGYGVTIFEAAPYAGGMLRTGIPAFRLPKDVLQQEIDAILNLGVELKLNSPIGEDLTLADLKAQGYESVFITI
GLQDPLRILNIEGTDLAGIYSGVEYVRDHEKIRLGKRCLVIGGGGVAIDCAQHAVRQGAEQVMIACLESWETMPASLTER
EDAQEEGIVFHPALGPRRFMGHNGHVARAEFLQVSSVFDSEGRFAPTFVPDSTTTLEVDSVILAVGQASTAPSLAGLAGL
DLTPNGLIKAAEDMSTNLPGVFAGGDVRWRFTRNATDAIADGQKAARAIHGYLSGHTPQVIRKGFMRPVSPDFENTRCDT
IAPVPIPKRDAGERVRTKEEITIGFGEAEAREQAARCRRCNIQTVFERSRCLRCGTCVDTCVNGALRIVRLADIQGDEDV
AKLTDALKKSAQTRSKAMTAIIKDESRCASCGMCARRCPGGAITMAEFYCQEEWE

Sequences:

>Translated_615_residues
MANPSGKYQVKILGIEDYQALTACQSACPLGTDTKRYVRAITEGDYEKAFLIARQTNPLVSVCSRVCTAPCEKSCRKGEQ
GHPVDIRALKRFACDKHGVTSPRAVAGRLEEISRQNGGVGDRTGNHILTMAAVQKAGAAATSRKPAPRVAIIGSGPTGLS
AAHDLALLGYGVTIFEAAPYAGGMLRTGIPAFRLPKDVLQQEIDAILNLGVELKLNSPIGEDLTLADLKAQGYESVFITI
GLQDPLRILNIEGTDLAGIYSGVEYVRDHEKIRLGKRCLVIGGGGVAIDCAQHAVRQGAEQVMIACLESWETMPASLTER
EDAQEEGIVFHPALGPRRFMGHNGHVARAEFLQVSSVFDSEGRFAPTFVPDSTTTLEVDSVILAVGQASTAPSLAGLAGL
DLTPNGLIKAAEDMSTNLPGVFAGGDVRWRFTRNATDAIADGQKAARAIHGYLSGHTPQVIRKGFMRPVSPDFENTRCDT
IAPVPIPKRDAGERVRTKEEITIGFGEAEAREQAARCRRCNIQTVFERSRCLRCGTCVDTCVNGALRIVRLADIQGDEDV
AKLTDALKKSAQTRSKAMTAIIKDESRCASCGMCARRCPGGAITMAEFYCQEEWE
>Mature_614_residues
ANPSGKYQVKILGIEDYQALTACQSACPLGTDTKRYVRAITEGDYEKAFLIARQTNPLVSVCSRVCTAPCEKSCRKGEQG
HPVDIRALKRFACDKHGVTSPRAVAGRLEEISRQNGGVGDRTGNHILTMAAVQKAGAAATSRKPAPRVAIIGSGPTGLSA
AHDLALLGYGVTIFEAAPYAGGMLRTGIPAFRLPKDVLQQEIDAILNLGVELKLNSPIGEDLTLADLKAQGYESVFITIG
LQDPLRILNIEGTDLAGIYSGVEYVRDHEKIRLGKRCLVIGGGGVAIDCAQHAVRQGAEQVMIACLESWETMPASLTERE
DAQEEGIVFHPALGPRRFMGHNGHVARAEFLQVSSVFDSEGRFAPTFVPDSTTTLEVDSVILAVGQASTAPSLAGLAGLD
LTPNGLIKAAEDMSTNLPGVFAGGDVRWRFTRNATDAIADGQKAARAIHGYLSGHTPQVIRKGFMRPVSPDFENTRCDTI
APVPIPKRDAGERVRTKEEITIGFGEAEAREQAARCRRCNIQTVFERSRCLRCGTCVDTCVNGALRIVRLADIQGDEDVA
KLTDALKKSAQTRSKAMTAIIKDESRCASCGMCARRCPGGAITMAEFYCQEEWE

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=457, Percent_Identity=29.1028446389497, Blast_Score=163, Evalue=5e-40,
Organism=Escherichia coli, GI1789606, Length=452, Percent_Identity=30.3097345132743, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1789243, Length=590, Percent_Identity=28.4745762711864, Blast_Score=170, Evalue=3e-43,
Organism=Escherichia coli, GI1788468, Length=435, Percent_Identity=30.8045977011494, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI87082180, Length=447, Percent_Identity=29.7539149888143, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI1788811, Length=454, Percent_Identity=27.3127753303965, Blast_Score=154, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI71984108, Length=461, Percent_Identity=31.0195227765727, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17570289, Length=475, Percent_Identity=25.8947368421053, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17543860, Length=165, Percent_Identity=33.9393939393939, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6320030, Length=475, Percent_Identity=25.6842105263158, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24665547, Length=467, Percent_Identity=29.3361884368308, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24665543, Length=467, Percent_Identity=29.3361884368308, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI28574881, Length=467, Percent_Identity=29.3361884368308, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24665539, Length=467, Percent_Identity=29.3361884368308, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24640763, Length=456, Percent_Identity=27.6315789473684, Blast_Score=136, Evalue=5e-32,
Organism=Drosophila melanogaster, GI18858217, Length=456, Percent_Identity=27.6315789473684, Blast_Score=136, Evalue=5e-32,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 66089; Mature: 65958

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANPSGKYQVKILGIEDYQALTACQSACPLGTDTKRYVRAITEGDYEKAFLIARQTNPLV
CCCCCCCEEEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCHHH
SVCSRVCTAPCEKSCRKGEQGHPVDIRALKRFACDKHGVTSPRAVAGRLEEISRQNGGVG
HHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCC
DRTGNHILTMAAVQKAGAAATSRKPAPRVAIIGSGPTGLSAAHDLALLGYGVTIFEAAPY
CCCCCEEEEHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCEEEEECCCC
AGGMLRTGIPAFRLPKDVLQQEIDAILNLGVELKLNSPIGEDLTLADLKAQGYESVFITI
CCCHHHCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEHHCCCCCCEEEEEE
GLQDPLRILNIEGTDLAGIYSGVEYVRDHEKIRLGKRCLVIGGGGVAIDCAQHAVRQGAE
ECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEHHHHHHHHHHHH
QVMIACLESWETMPASLTEREDAQEEGIVFHPALGPRRFMGHNGHVARAEFLQVSSVFDS
HHHHHHHHHHCCCCCCHHHHCCHHHCCEEEECCCCCHHHCCCCCCCHHHHHHHHHHHHCC
EGRFAPTFVPDSTTTLEVDSVILAVGQASTAPSLAGLAGLDLTPNGLIKAAEDMSTNLPG
CCCCCCEECCCCCCEEEHHHEEEEECCCCCCCCHHCCCCCCCCCCHHHHHHHHHCCCCCE
VFAGGDVRWRFTRNATDAIADGQKAARAIHGYLSGHTPQVIRKGFMRPVSPDFENTRCDT
EEECCCEEEEEECCCHHHHHCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCE
IAPVPIPKRDAGERVRTKEEITIGFGEAEAREQAARCRRCNIQTVFERSRCLRCGTCVDT
ECCCCCCCCCCCCHHCCCCEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
CVNGALRIVRLADIQGDEDVAKLTDALKKSAQTRSKAMTAIIKDESRCASCGMCARRCPG
HHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
GAITMAEFYCQEEWE
CCEEHHHHHHHCCCC
>Mature Secondary Structure 
ANPSGKYQVKILGIEDYQALTACQSACPLGTDTKRYVRAITEGDYEKAFLIARQTNPLV
CCCCCCEEEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCHHH
SVCSRVCTAPCEKSCRKGEQGHPVDIRALKRFACDKHGVTSPRAVAGRLEEISRQNGGVG
HHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCC
DRTGNHILTMAAVQKAGAAATSRKPAPRVAIIGSGPTGLSAAHDLALLGYGVTIFEAAPY
CCCCCEEEEHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCEEEEECCCC
AGGMLRTGIPAFRLPKDVLQQEIDAILNLGVELKLNSPIGEDLTLADLKAQGYESVFITI
CCCHHHCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEHHCCCCCCEEEEEE
GLQDPLRILNIEGTDLAGIYSGVEYVRDHEKIRLGKRCLVIGGGGVAIDCAQHAVRQGAE
ECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEHHHHHHHHHHHH
QVMIACLESWETMPASLTEREDAQEEGIVFHPALGPRRFMGHNGHVARAEFLQVSSVFDS
HHHHHHHHHHCCCCCCHHHHCCHHHCCEEEECCCCCHHHCCCCCCCHHHHHHHHHHHHCC
EGRFAPTFVPDSTTTLEVDSVILAVGQASTAPSLAGLAGLDLTPNGLIKAAEDMSTNLPG
CCCCCCEECCCCCCEEEHHHEEEEECCCCCCCCHHCCCCCCCCCCHHHHHHHHHCCCCCE
VFAGGDVRWRFTRNATDAIADGQKAARAIHGYLSGHTPQVIRKGFMRPVSPDFENTRCDT
EEECCCEEEEEECCCHHHHHCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCE
IAPVPIPKRDAGERVRTKEEITIGFGEAEAREQAARCRRCNIQTVFERSRCLRCGTCVDT
ECCCCCCCCCCCCHHCCCCEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
CVNGALRIVRLADIQGDEDVAKLTDALKKSAQTRSKAMTAIIKDESRCASCGMCARRCPG
HHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
GAITMAEFYCQEEWE
CCEEHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]