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The map label for this gene is fhlA [H]

Identifier: 78223316

GI number: 78223316

Start: 2374764

End: 2376185

Strand: Direct

Name: fhlA [H]

Synonym: Gmet_2109

Alternate gene names: 78223316

Gene position: 2374764-2376185 (Clockwise)

Preceding gene: 78223315

Following gene: 78223317

Centisome position: 59.41

GC content: 58.3

Gene sequence:

>1422_bases
ATGGCCGACACAGAGCAGCATCTGGAAGAGATGCTTCGGCTGGCAAATTTCACCATAGAGCGGACCGGCACCCCCATTGT
CTGGGTGGACTCCACGACCCGTTTTGCACGGGTAAACGACGCGGCATGCCGGTGTTTCGGTTACTCGCCCGATGAATTCA
TCCGCCTGACTGCCCAGGACATCGCCCCGGGGATCCCCACGGAATTGTGGCCGCAGCACTGGGCAGAGCTGAAGCGGATG
CAATTCCGCACCTTTTCGGATCATTTCAGAAAACGCTCCGGGGAAAAGTTCCCGGTAGAGGTCGAGGAAAACTTCATCCG
GTTCGAAGGGAGGGAATACTCCTGCAGCTTCATCAAGGACATGACCCAGCATTTCCAGACCCAGGAAGCCCTGAGGCGGG
CCTTGGACGAGGTGCAAACGCTCAAAAACCGTCTGGAAGCCGAAAACGTGTACCTGATGAACGAGATCAAACTCTCCAAC
AACTTCGATGAAATAGTCACCGGCAATCCCATCTTCATGAAGGCCCTGCAGCTTGTGGAACAGGTTGCCCCCACCGATGC
GTCCGTACTTATCCTCGGAGAAACAGGCGTCGGCAAGGAACTCATCGCCCGCGCCATCCATAACCTGAGCAACCGCCGTG
ATCGCCCCCTCATCCGGCTCAATTGCGCCGCATTGCCGGCAACTCTCATGGAAAACGAACTTTTCGGCCACGAAAGGGGA
GCCTTCACGGGAGCGAACTGCCGCAAACCCGGCAGGTTCGACCTGGCTGACGGGGGGACCCTTTTTCTCGACGAGGTGGG
TGATCTGCCCATGGAGTTGCAGAGCAAGCTTCTGCGTGTCTTGCAGGAAGGGGAATTCGAGCGGCTCGGCGGCGCTGAAA
CGATAAAGGTCGATGTACGCATCCTGGCCGCGACCCACGTGGATTTGAAAGCCCTGCTTGCCGAGGGGCGGTTTCGCAGC
GACCTGTACTATCGCCTCAATGTCTTTCCGATCCAATGCCCCCCCCTCAGGGAACGCAGGGAAGACATCCCCCTGCTGGT
CAGGCATTTTGTCAAAGCCTCCTGCGAAAAGAACGGAAAAAGCATCGAGCTTATTCCGCGCCATGTCATGGAGTCACTGG
AGGGGTACCCCTGGCCCGGCAATATCCGGGAACTGCAGAACGTTATCGAACGGGCGGTGATCATCAGCCGGGGAAACAAG
CTGGAGATCGACTCCCTCCCTAAAATGGTCATAGCCGCCGATGCAGCCCCCATCTCACCTCTGGAAGTGACCGAACGGGA
ACATATCATCCGTGCTCTCGAAACAACGGCATGGCGAGTCGGCGGGCCAAAGGGCGCCGCTGAAATCCTCGGACTGAAAC
GCACAACCCTCCAGGCGAGGATGCGCAAGCTCGGCATCAACCGGCAGGGCCTGCCAGCCTGA

Upstream 100 bases:

>100_bases
TTCCTGGTTTTTCACCGAAACCGGCAGGCAACTGGCCTGGGCAGCGTTGGAAAAGGCGGGATACACACCTTGACCGCGCT
TCAGTGCGCTGGGGGTTTCC

Downstream 100 bases:

>100_bases
CCGCACGCACACTGACTCTCATCCTCCTTCTCCCCCTCCCCTTTCCCGCACGCTGTCTCCCGAGCAAACGCCCAAGTTTT
TCAGAAGATATCGTTCTATT

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MADTEQHLEEMLRLANFTIERTGTPIVWVDSTTRFARVNDAACRCFGYSPDEFIRLTAQDIAPGIPTELWPQHWAELKRM
QFRTFSDHFRKRSGEKFPVEVEENFIRFEGREYSCSFIKDMTQHFQTQEALRRALDEVQTLKNRLEAENVYLMNEIKLSN
NFDEIVTGNPIFMKALQLVEQVAPTDASVLILGETGVGKELIARAIHNLSNRRDRPLIRLNCAALPATLMENELFGHERG
AFTGANCRKPGRFDLADGGTLFLDEVGDLPMELQSKLLRVLQEGEFERLGGAETIKVDVRILAATHVDLKALLAEGRFRS
DLYYRLNVFPIQCPPLRERREDIPLLVRHFVKASCEKNGKSIELIPRHVMESLEGYPWPGNIRELQNVIERAVIISRGNK
LEIDSLPKMVIAADAAPISPLEVTEREHIIRALETTAWRVGGPKGAAEILGLKRTTLQARMRKLGINRQGLPA

Sequences:

>Translated_473_residues
MADTEQHLEEMLRLANFTIERTGTPIVWVDSTTRFARVNDAACRCFGYSPDEFIRLTAQDIAPGIPTELWPQHWAELKRM
QFRTFSDHFRKRSGEKFPVEVEENFIRFEGREYSCSFIKDMTQHFQTQEALRRALDEVQTLKNRLEAENVYLMNEIKLSN
NFDEIVTGNPIFMKALQLVEQVAPTDASVLILGETGVGKELIARAIHNLSNRRDRPLIRLNCAALPATLMENELFGHERG
AFTGANCRKPGRFDLADGGTLFLDEVGDLPMELQSKLLRVLQEGEFERLGGAETIKVDVRILAATHVDLKALLAEGRFRS
DLYYRLNVFPIQCPPLRERREDIPLLVRHFVKASCEKNGKSIELIPRHVMESLEGYPWPGNIRELQNVIERAVIISRGNK
LEIDSLPKMVIAADAAPISPLEVTEREHIIRALETTAWRVGGPKGAAEILGLKRTTLQARMRKLGINRQGLPA
>Mature_472_residues
ADTEQHLEEMLRLANFTIERTGTPIVWVDSTTRFARVNDAACRCFGYSPDEFIRLTAQDIAPGIPTELWPQHWAELKRMQ
FRTFSDHFRKRSGEKFPVEVEENFIRFEGREYSCSFIKDMTQHFQTQEALRRALDEVQTLKNRLEAENVYLMNEIKLSNN
FDEIVTGNPIFMKALQLVEQVAPTDASVLILGETGVGKELIARAIHNLSNRRDRPLIRLNCAALPATLMENELFGHERGA
FTGANCRKPGRFDLADGGTLFLDEVGDLPMELQSKLLRVLQEGEFERLGGAETIKVDVRILAATHVDLKALLAEGRFRSD
LYYRLNVFPIQCPPLRERREDIPLLVRHFVKASCEKNGKSIELIPRHVMESLEGYPWPGNIRELQNVIERAVIISRGNKL
EIDSLPKMVIAADAAPISPLEVTEREHIIRALETTAWRVGGPKGAAEILGLKRTTLQARMRKLGINRQGLPA

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=344, Percent_Identity=54.0697674418605, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI87082117, Length=372, Percent_Identity=47.3118279569892, Blast_Score=337, Evalue=1e-93,
Organism=Escherichia coli, GI1790437, Length=305, Percent_Identity=49.8360655737705, Blast_Score=284, Evalue=8e-78,
Organism=Escherichia coli, GI1788550, Length=347, Percent_Identity=44.956772334294, Blast_Score=278, Evalue=6e-76,
Organism=Escherichia coli, GI1790299, Length=338, Percent_Identity=40.5325443786982, Blast_Score=254, Evalue=9e-69,
Organism=Escherichia coli, GI1788905, Length=232, Percent_Identity=50.4310344827586, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1789233, Length=321, Percent_Identity=41.4330218068536, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI87081872, Length=313, Percent_Identity=43.7699680511182, Blast_Score=234, Evalue=8e-63,
Organism=Escherichia coli, GI87082152, Length=222, Percent_Identity=50.9009009009009, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1786524, Length=332, Percent_Identity=37.9518072289157, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1787583, Length=453, Percent_Identity=30.0220750551876, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI87081858, Length=320, Percent_Identity=34.6875, Blast_Score=168, Evalue=6e-43,
Organism=Escherichia coli, GI1789828, Length=249, Percent_Identity=34.9397590361446, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53733; Mature: 53601

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS50112 PAS ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTEQHLEEMLRLANFTIERTGTPIVWVDSTTRFARVNDAACRCFGYSPDEFIRLTAQD
CCCHHHHHHHHHHHHCCEEECCCCCEEEECCCCCEEECCCHHHEECCCCHHHHEEEEHHH
IAPGIPTELWPQHWAELKRMQFRTFSDHFRKRSGEKFPVEVEENFIRFEGREYSCSFIKD
CCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCEEEECCCCCCHHHHHH
MTQHFQTQEALRRALDEVQTLKNRLEAENVYLMNEIKLSNNFDEIVTGNPIFMKALQLVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCHHHHCCCHHHHHHHHHHH
QVAPTDASVLILGETGVGKELIARAIHNLSNRRDRPLIRLNCAALPATLMENELFGHERG
HHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHCCCCCC
AFTGANCRKPGRFDLADGGTLFLDEVGDLPMELQSKLLRVLQEGEFERLGGAETIKVDVR
CCCCCCCCCCCCEECCCCCEEEECCCCCCCHHHHHHHHHHHHCCCHHHCCCCEEEEEEEE
ILAATHVDLKALLAEGRFRSDLYYRLNVFPIQCPPLRERREDIPLLVRHFVKASCEKNGK
EEEEHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCHHHHHCCHHHHHHHHHHHHHHCCCC
SIELIPRHVMESLEGYPWPGNIRELQNVIERAVIISRGNKLEIDSLPKMVIAADAAPISP
EEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHCCCCEEEEECCCCCCC
LEVTEREHIIRALETTAWRVGGPKGAAEILGLKRTTLQARMRKLGINRQGLPA
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ADTEQHLEEMLRLANFTIERTGTPIVWVDSTTRFARVNDAACRCFGYSPDEFIRLTAQD
CCHHHHHHHHHHHHCCEEECCCCCEEEECCCCCEEECCCHHHEECCCCHHHHEEEEHHH
IAPGIPTELWPQHWAELKRMQFRTFSDHFRKRSGEKFPVEVEENFIRFEGREYSCSFIKD
CCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCEEEECCCCCCHHHHHH
MTQHFQTQEALRRALDEVQTLKNRLEAENVYLMNEIKLSNNFDEIVTGNPIFMKALQLVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCHHHHCCCHHHHHHHHHHH
QVAPTDASVLILGETGVGKELIARAIHNLSNRRDRPLIRLNCAALPATLMENELFGHERG
HHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHCCCCCC
AFTGANCRKPGRFDLADGGTLFLDEVGDLPMELQSKLLRVLQEGEFERLGGAETIKVDVR
CCCCCCCCCCCCEECCCCCEEEECCCCCCCHHHHHHHHHHHHCCCHHHCCCCEEEEEEEE
ILAATHVDLKALLAEGRFRSDLYYRLNVFPIQCPPLRERREDIPLLVRHFVKASCEKNGK
EEEEHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCHHHHHCCHHHHHHHHHHHHHHCCCC
SIELIPRHVMESLEGYPWPGNIRELQNVIERAVIISRGNKLEIDSLPKMVIAADAAPISP
EEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHCCCCEEEEECCCCCCC
LEVTEREHIIRALETTAWRVGGPKGAAEILGLKRTTLQARMRKLGINRQGLPA
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]