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The map label for this gene is iclR [H]

Identifier: 78223271

GI number: 78223271

Start: 2320696

End: 2321475

Strand: Reverse

Name: iclR [H]

Synonym: Gmet_2064

Alternate gene names: 78223271

Gene position: 2321475-2320696 (Counterclockwise)

Preceding gene: 78223272

Following gene: 78223270

Centisome position: 58.07

GC content: 59.36

Gene sequence:

>780_bases
ATGAAGACAAAAAACAAAACGGTGCCGTTGATAAAATCGGTGGACCATGCTTTCGAGCTGCTGGAGCAGTTCCGGGGCGA
TGCCAGCGAACTCAGCCTGAGCGAGCTGGGGAGGCGCCTCAACCTTCCCAAGAACAACGTGTTCCGGCTCCTGGCCACCC
TCGAATCCAGGAGATTCATCGAGCATAACACCTCTACCGGCAGCTACCGGCTCGGCCTCAAGGCCTTTCACCTGAGCCGG
ATGGTTTCACGGCAGATGAGGCTTGTCAACCGCGCACGACCCACTTTGGAGGCCATGGTCAGGGAATGCAACGAGACGGT
CTGCCTGACGGTCCTGAGGGATTTTTCCATTGTCTGCCTCGATGCGGTGGAGTGCAGCCAGCCGGTGCGGGTCGTACCCC
CCATCGGCATGAGGCTTCCCGCCCATTGCACGGCGGCCGGCAAGGTACAGCTGGCGCACCTGCCGGAGGACAAGGTGGCA
AAACTGCTCACCGTCCATAAGCTCGAGCGGAACACGCCGTACACAATCATTGACCCCGCAAGCTTCAAGGAGCACCTCCT
CCAGATAAAAGAACACGGGTATGCCATCGGTCGGGAAGAGCTGGAAGTGGGAGTCACCTGTGTCGCGACTCCGGTGCGCG
ATTACAGCTCCAACGTCATCGGGGCGATTGTCCTTCCCGGCCCCATCACGCGGTTCAGCGATGATCTGATCAACGGGGTG
CTCATCCCCCTCGCGAAAAAAGGGGCTGAGGAAATTTCCAGCCAGCTCGGGTATTGCTGA

Upstream 100 bases:

>100_bases
CCGAGGTGATGGCCGAGGCCATGGCGTAAGGGGAATCCCCCGGCACTGCCGGGGGATTGCTTTGTTGTACTGTTCCTGAC
AGCCGGAGGGAACACGACCC

Downstream 100 bases:

>100_bases
AGCGGGTTCATGGGGAGACGCGCGTGTCCGGGACACTGTTTACCTATCGGAGAAAAGTCGATTTTGGAGACTGCGACTCC
GCCCGCATCTACTTCACGCC

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MKTKNKTVPLIKSVDHAFELLEQFRGDASELSLSELGRRLNLPKNNVFRLLATLESRRFIEHNTSTGSYRLGLKAFHLSR
MVSRQMRLVNRARPTLEAMVRECNETVCLTVLRDFSIVCLDAVECSQPVRVVPPIGMRLPAHCTAAGKVQLAHLPEDKVA
KLLTVHKLERNTPYTIIDPASFKEHLLQIKEHGYAIGREELEVGVTCVATPVRDYSSNVIGAIVLPGPITRFSDDLINGV
LIPLAKKGAEEISSQLGYC

Sequences:

>Translated_259_residues
MKTKNKTVPLIKSVDHAFELLEQFRGDASELSLSELGRRLNLPKNNVFRLLATLESRRFIEHNTSTGSYRLGLKAFHLSR
MVSRQMRLVNRARPTLEAMVRECNETVCLTVLRDFSIVCLDAVECSQPVRVVPPIGMRLPAHCTAAGKVQLAHLPEDKVA
KLLTVHKLERNTPYTIIDPASFKEHLLQIKEHGYAIGREELEVGVTCVATPVRDYSSNVIGAIVLPGPITRFSDDLINGV
LIPLAKKGAEEISSQLGYC
>Mature_259_residues
MKTKNKTVPLIKSVDHAFELLEQFRGDASELSLSELGRRLNLPKNNVFRLLATLESRRFIEHNTSTGSYRLGLKAFHLSR
MVSRQMRLVNRARPTLEAMVRECNETVCLTVLRDFSIVCLDAVECSQPVRVVPPIGMRLPAHCTAAGKVQLAHLPEDKVA
KLLTVHKLERNTPYTIIDPASFKEHLLQIKEHGYAIGREELEVGVTCVATPVRDYSSNVIGAIVLPGPITRFSDDLINGV
LIPLAKKGAEEISSQLGYC

Specific function: Regulation of the glyoxylate bypass operon (aceBAK), which encodes isocitrate lyase, malate synthase as well as isocitrate dehydrogenase kinase/phosphorylase. Glyoxylate disrupts the interaction with the promoter by favoring the inactive dimeric form. Pyr

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI87082359, Length=241, Percent_Identity=31.5352697095436, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1786468, Length=251, Percent_Identity=32.2709163346614, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1786715, Length=248, Percent_Identity=27.8225806451613, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1788131, Length=248, Percent_Identity=30.241935483871, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1789999, Length=247, Percent_Identity=30.7692307692308, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1790752, Length=230, Percent_Identity=31.304347826087, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 28837; Mature: 28837

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKNKTVPLIKSVDHAFELLEQFRGDASELSLSELGRRLNLPKNNVFRLLATLESRRFI
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHH
EHNTSTGSYRLGLKAFHLSRMVSRQMRLVNRARPTLEAMVRECNETVCLTVLRDFSIVCL
HCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHCCCEEEE
DAVECSQPVRVVPPIGMRLPAHCTAAGKVQLAHLPEDKVAKLLTVHKLERNTPYTIIDPA
EHHHCCCCCEECCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEECCH
SFKEHLLQIKEHGYAIGREELEVGVTCVATPVRDYSSNVIGAIVLPGPITRFSDDLINGV
HHHHHHHHHHHCCCCCCHHHHHHCEEEEECCCHHHCCCCEEEEEECCCCHHHHHHHHHHE
LIPLAKKGAEEISSQLGYC
EHHHHHCCHHHHHHHCCCC
>Mature Secondary Structure
MKTKNKTVPLIKSVDHAFELLEQFRGDASELSLSELGRRLNLPKNNVFRLLATLESRRFI
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHH
EHNTSTGSYRLGLKAFHLSRMVSRQMRLVNRARPTLEAMVRECNETVCLTVLRDFSIVCL
HCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHCCCEEEE
DAVECSQPVRVVPPIGMRLPAHCTAAGKVQLAHLPEDKVAKLLTVHKLERNTPYTIIDPA
EHHHCCCCCEECCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEECCH
SFKEHLLQIKEHGYAIGREELEVGVTCVATPVRDYSSNVIGAIVLPGPITRFSDDLINGV
HHHHHHHHHHHCCCCCCHHHHHHCEEEEECCCHHHCCCCEEEEEECCCCHHHHHHHHHHE
LIPLAKKGAEEISSQLGYC
EHHHHHCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185227; 1995431; 8265357; 9278503; 1995429 [H]