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The map label for this gene is paaJ [H]

Identifier: 78223265

GI number: 78223265

Start: 2312207

End: 2313406

Strand: Reverse

Name: paaJ [H]

Synonym: Gmet_2058

Alternate gene names: 78223265

Gene position: 2313406-2312207 (Counterclockwise)

Preceding gene: 78223266

Following gene: 78223264

Centisome position: 57.87

GC content: 61.33

Gene sequence:

>1200_bases
ATGCGTGAAGCAGTAATTGTCGATGCTGTCCGTACTCCGGTGGGAAAATTCAACGGCGCCCTGAAAAACGTCCGCTCTGA
CGACCTGGCCGCCCACTGTATTTCCGAACTGGTGAAGCGTAACAATCTTGATCCGAACCTGGTCGAAGATGTGGTGCTCG
GTTGCACCAACCAGGCGGGCGAGGACAACCGGAACGTCGGCCGGATGGCGGCGCTTCTGGCCGGTCTGCCGTATTCGGTC
GCGGGGCAGACCATCAACCGTCTCTGTGCCTCGGGCCTGAATGCCATCAACAGCGCAGCCCATGCGATTAAACTCGGCGA
AGGTGATGTCTTTATCGCTGGCGGTACCGAATCCATGACCCGTGCCCCCTTTGTCATGGCCAAGTCCGAATCCCCTTTCT
CGCGCGATATCAGGGTGTTTGACAGCGTCATCGGCTGGCGGTTCACCAACCCGAAGATGACTGAACCATATGCCAAGGAA
GGAATGGGCGAAACCGCCGAGAACGTGGCGGTGCGGTATGGCCTCACCCGCCAGGAGCAGGACGAGTTTGCCCTGGAGAC
CCAACGCAAATGGGCTGCCGCCGATGCGGCCGGCAAGTTCAATGACGAGATCGTTCCCGTCGTTATCCCCCAGAAGAAGG
GGGATCCGATCATCGTCTCCAGGGATGAATTCCCTCGCGGCAACGATGTCACCATGGAGCAGCTTGCCAAGCTGCCGGCT
GCCTTCAGAAAGGAGGGCACCGTCACCGCCGGCAACTCCAGCGGCATCAACGACGGCGCCGCAGCGCTCCTCCTCATGGA
GGCAGAAACCGCCAAGAAGCTCGGCTACAAGCCGCTTGCCAGGGTCGTCGCCAGTGCGGTTGCCGGTTGCGATCCCTCGT
ACATGGGGCTCGGCCCCATCCCGGCGATCCAGAAGGTGCTGCAACGGTCCGGCCTGAAAATCGAAGATATTGACCTCTTC
GAGCTGAACGAGGCCTTTGCCGCCCAGTCCATCCCCTGCATCCGCGAACTGGGGATCGATCCGGCCAAGGTGAACGTCAA
CGGCGGCTCCATCGCCATCGGCCACCCCCTCGGCTCCACCGGCGCCCGGATCACCGCCACGCTGGTCCATGAGATGAAGC
GCCGTGGCTCCCGCTACGGTCTCGTGTCCCTCTGTATCGGTGTCGGACAGGGAATTGCGACGATCTTCGAACGCGTGTAA

Upstream 100 bases:

>100_bases
TGCTCCGAGAAGTCCCGCCACATCACCGGCGAATGCATCAAGGTTGATGGCGGTCAGTACATCTAATTAACTCTTAATTC
GCAAAGCGATGGAGGTTCCA

Downstream 100 bases:

>100_bases
GTGAACAATGGGAGGTGGCATGGAGATCATGTCACCTCTTTTTTTATGGGAGTTTGATATCCAGCATGAACGGTGAAGCA
TTAACTATTGCGGCTGTGCC

Product: thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MREAVIVDAVRTPVGKFNGALKNVRSDDLAAHCISELVKRNNLDPNLVEDVVLGCTNQAGEDNRNVGRMAALLAGLPYSV
AGQTINRLCASGLNAINSAAHAIKLGEGDVFIAGGTESMTRAPFVMAKSESPFSRDIRVFDSVIGWRFTNPKMTEPYAKE
GMGETAENVAVRYGLTRQEQDEFALETQRKWAAADAAGKFNDEIVPVVIPQKKGDPIIVSRDEFPRGNDVTMEQLAKLPA
AFRKEGTVTAGNSSGINDGAAALLLMEAETAKKLGYKPLARVVASAVAGCDPSYMGLGPIPAIQKVLQRSGLKIEDIDLF
ELNEAFAAQSIPCIRELGIDPAKVNVNGGSIAIGHPLGSTGARITATLVHEMKRRGSRYGLVSLCIGVGQGIATIFERV

Sequences:

>Translated_399_residues
MREAVIVDAVRTPVGKFNGALKNVRSDDLAAHCISELVKRNNLDPNLVEDVVLGCTNQAGEDNRNVGRMAALLAGLPYSV
AGQTINRLCASGLNAINSAAHAIKLGEGDVFIAGGTESMTRAPFVMAKSESPFSRDIRVFDSVIGWRFTNPKMTEPYAKE
GMGETAENVAVRYGLTRQEQDEFALETQRKWAAADAAGKFNDEIVPVVIPQKKGDPIIVSRDEFPRGNDVTMEQLAKLPA
AFRKEGTVTAGNSSGINDGAAALLLMEAETAKKLGYKPLARVVASAVAGCDPSYMGLGPIPAIQKVLQRSGLKIEDIDLF
ELNEAFAAQSIPCIRELGIDPAKVNVNGGSIAIGHPLGSTGARITATLVHEMKRRGSRYGLVSLCIGVGQGIATIFERV
>Mature_399_residues
MREAVIVDAVRTPVGKFNGALKNVRSDDLAAHCISELVKRNNLDPNLVEDVVLGCTNQAGEDNRNVGRMAALLAGLPYSV
AGQTINRLCASGLNAINSAAHAIKLGEGDVFIAGGTESMTRAPFVMAKSESPFSRDIRVFDSVIGWRFTNPKMTEPYAKE
GMGETAENVAVRYGLTRQEQDEFALETQRKWAAADAAGKFNDEIVPVVIPQKKGDPIIVSRDEFPRGNDVTMEQLAKLPA
AFRKEGTVTAGNSSGINDGAAALLLMEAETAKKLGYKPLARVVASAVAGCDPSYMGLGPIPAIQKVLQRSGLKIEDIDLF
ELNEAFAAQSIPCIRELGIDPAKVNVNGGSIAIGHPLGSTGARITATLVHEMKRRGSRYGLVSLCIGVGQGIATIFERV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=399, Percent_Identity=42.8571428571429, Blast_Score=335, Evalue=4e-92,
Organism=Homo sapiens, GI148539872, Length=396, Percent_Identity=42.6767676767677, Blast_Score=308, Evalue=4e-84,
Organism=Homo sapiens, GI4557237, Length=401, Percent_Identity=39.6508728179551, Blast_Score=275, Evalue=4e-74,
Organism=Homo sapiens, GI4501853, Length=401, Percent_Identity=41.3965087281795, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI4504327, Length=428, Percent_Identity=32.9439252336449, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI194353979, Length=89, Percent_Identity=56.1797752808989, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1787663, Length=402, Percent_Identity=56.2189054726368, Blast_Score=453, Evalue=1e-129,
Organism=Escherichia coli, GI1788554, Length=402, Percent_Identity=48.7562189054726, Blast_Score=335, Evalue=3e-93,
Organism=Escherichia coli, GI87082165, Length=407, Percent_Identity=43.4889434889435, Blast_Score=318, Evalue=3e-88,
Organism=Escherichia coli, GI48994986, Length=408, Percent_Identity=44.3627450980392, Blast_Score=312, Evalue=2e-86,
Organism=Escherichia coli, GI1788683, Length=412, Percent_Identity=33.0097087378641, Blast_Score=195, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI133906874, Length=399, Percent_Identity=47.1177944862155, Blast_Score=351, Evalue=3e-97,
Organism=Caenorhabditis elegans, GI25147385, Length=400, Percent_Identity=36.5, Blast_Score=260, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI17535921, Length=400, Percent_Identity=34.25, Blast_Score=236, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI17551802, Length=428, Percent_Identity=31.3084112149533, Blast_Score=189, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17535917, Length=407, Percent_Identity=29.2383292383292, Blast_Score=163, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6322031, Length=405, Percent_Identity=41.2345679012346, Blast_Score=262, Evalue=7e-71,
Organism=Saccharomyces cerevisiae, GI6325229, Length=400, Percent_Identity=36.25, Blast_Score=230, Evalue=4e-61,
Organism=Drosophila melanogaster, GI24655093, Length=399, Percent_Identity=43.3583959899749, Blast_Score=324, Evalue=7e-89,
Organism=Drosophila melanogaster, GI17648125, Length=400, Percent_Identity=41.5, Blast_Score=295, Evalue=6e-80,
Organism=Drosophila melanogaster, GI24640423, Length=403, Percent_Identity=39.9503722084367, Blast_Score=283, Evalue=1e-76,
Organism=Drosophila melanogaster, GI17137578, Length=425, Percent_Identity=32.9411764705882, Blast_Score=188, Evalue=5e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 42504; Mature: 42504

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREAVIVDAVRTPVGKFNGALKNVRSDDLAAHCISELVKRNNLDPNLVEDVVLGCTNQAG
CCCEEEEHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
EDNRNVGRMAALLAGLPYSVAGQTINRLCASGLNAINSAAHAIKLGEGDVFIAGGTESMT
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEECCCCCHH
RAPFVMAKSESPFSRDIRVFDSVIGWRFTNPKMTEPYAKEGMGETAENVAVRYGLTRQEQ
CCCEEEECCCCCCHHHHHHHHHHHCCEECCCCCCCCHHHHCCCCHHHHHHHHCCCCCCCH
DEFALETQRKWAAADAAGKFNDEIVPVVIPQKKGDPIIVSRDEFPRGNDVTMEQLAKLPA
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCHHHHHHHHH
AFRKEGTVTAGNSSGINDGAAALLLMEAETAKKLGYKPLARVVASAVAGCDPSYMGLGPI
HHHCCCCEECCCCCCCCCCCCEEEEEEHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCC
PAIQKVLQRSGLKIEDIDLFELNEAFAAQSIPCIRELGIDPAKVNVNGGSIAIGHPLGST
HHHHHHHHHCCCCEECCCHHHHHHHHHHHCCCHHHHHCCCCEEEEECCCEEEECCCCCCC
GARITATLVHEMKRRGSRYGLVSLCIGVGQGIATIFERV
CCHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MREAVIVDAVRTPVGKFNGALKNVRSDDLAAHCISELVKRNNLDPNLVEDVVLGCTNQAG
CCCEEEEHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
EDNRNVGRMAALLAGLPYSVAGQTINRLCASGLNAINSAAHAIKLGEGDVFIAGGTESMT
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEECCCCCHH
RAPFVMAKSESPFSRDIRVFDSVIGWRFTNPKMTEPYAKEGMGETAENVAVRYGLTRQEQ
CCCEEEECCCCCCHHHHHHHHHHHCCEECCCCCCCCHHHHCCCCHHHHHHHHCCCCCCCH
DEFALETQRKWAAADAAGKFNDEIVPVVIPQKKGDPIIVSRDEFPRGNDVTMEQLAKLPA
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCHHHHHHHHH
AFRKEGTVTAGNSSGINDGAAALLLMEAETAKKLGYKPLARVVASAVAGCDPSYMGLGPI
HHHCCCCEECCCCCCCCCCCCEEEEEEHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCC
PAIQKVLQRSGLKIEDIDLFELNEAFAAQSIPCIRELGIDPAKVNVNGGSIAIGHPLGST
HHHHHHHHHCCCCEECCCHHHHHHHHHHHCCCHHHHHCCCCEEEEECCCEEEECCCCCCC
GARITATLVHEMKRRGSRYGLVSLCIGVGQGIATIFERV
CCHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 10766858 [H]