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The map label for this gene is rffA [C]

Identifier: 78223223

GI number: 78223223

Start: 2255485

End: 2256651

Strand: Reverse

Name: rffA [C]

Synonym: Gmet_2016

Alternate gene names: 78223223

Gene position: 2256651-2255485 (Counterclockwise)

Preceding gene: 78223224

Following gene: 78223222

Centisome position: 56.45

GC content: 49.87

Gene sequence:

>1167_bases
ATGAAGGTTAGCAGCAAGCCATCACTGACATGGGAAATTCTGACCTCCCGAGCCGATCGTCCCCGGCAGGAGGTGTTTCC
CTTTAATACCGGCAGATGTGAGTATTTTTACTCTGCTCGGTATGCCCTTGCGGCAGCACTCGATGCTCTGAAGCTTTCTC
CTCAGCAGTCGATTCTCATGCCCTCCTACAACTGCTGGGTTGAAGTTGAACCGGCTGTGCGTTCGGGCGCGAAGATTGAC
TGGTATAGGGTCAAGACCGATTTTTCAATAGATGAAGATGATCTTTTGGCTCGCATCCGGCCGGAGACGGCAGCGATCTT
TGTCATCCACTACCTCGGGTTTCCTCAGCGCCTGGAGAACATTCACCGGATATGCACCGAGCGGGGGATTGTTCTTATCG
AAGACTGCGCCCATGCGTTGCTTAGTAACGATGGCGACGTTCCTCTCGGCTTAACGGGTGACATGGCAATCTTCAGCATT
CGAAAGACCGTTCCCATTCCCGATGGCGGCTGTCTGTTGATCAATAACCCGGCCTTCTCTGTCAATCGGCCTCGAAGGGT
TCGCCCAAATCTATTCGCCACCTACTTCGTTGTTTCGGAAATGCTTGCGCGGGGATCAACTACTGAACCAAAACGTGTGA
ATCAGATGGCCTATTCGTGCAATTTCGCCTTTGCACGCCTGGCTCGAATGGGGCTTAGGGGAATTCACAAAATTTTTCAG
GACCATGGAGATTATCTGGTTTATCCAAGCGGTAATCATTTCAATGAGCAGGTGAAGCATTGGATGATGTCGGACGTTTC
ATCCGCAATACTTCGTGGAACTGAATTTCAACGAATCAAGGAGCGCCGCAGAGCGAACTTTCTCCACCTGCTCGACAAGT
TGATAATGAATGATCGCATAACGTTGCCGATACTTTCTCTTCCCGAAGGTGTTAGCCCATTGTTTTTCCCGGTGATCGTC
CAGGATAGGGACAGAGTTTACCGTCAACTTAAAGATCAGGGATTGTCCGGACATGATTGGTGGGGGGATTTTCATCCGGC
AGTCCCGTGGGATAAATACCCTGATGCTGTTTATCTGAAAACAAATGTATTTGGTTTTCCTGTTCACCAGGATCTTTCTA
GGGAGAGTCTGGGAATAATGCTCGCGGAATTCAGTCAGTGCTTGTGA

Upstream 100 bases:

>100_bases
CTGTTTAACAAAACCATCAAGGGCGTTTTGGCCCATTTTGCCTACACAAAAGTCAACCCGATTGTGAAACGATTTGGCAA
ACGGCTTGAGGAAGAAGGGC

Downstream 100 bases:

>100_bases
TGCTCGGAAGGTAGGACGGTGAAATCGGTGCTTTTGGGTAACCATTTGATATTACAAAGAGAATCAACAGGAGGTTGAAA
ATGAATAGAACATTTGCGCT

Product: DegT/DnrJ/EryC1/StrS aminotransferase

Products: TDP-D-fucosamine; 2-oxoglutarate [C]

Alternate protein names: TDP-4-Oxo-6-Deoxy-D-Glucose Transaminase; Aminotransferase; Degt/Dnrj/Eryc1/Strs Aminotransferase; Aminotransferase Degt/Dnrj/Eryc1/Strs Family; TDP-4-Keto-6-Deoxy-D-Glucose Transaminase Subfamily; Perosamine Synthetase-Related Protein

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MKVSSKPSLTWEILTSRADRPRQEVFPFNTGRCEYFYSARYALAAALDALKLSPQQSILMPSYNCWVEVEPAVRSGAKID
WYRVKTDFSIDEDDLLARIRPETAAIFVIHYLGFPQRLENIHRICTERGIVLIEDCAHALLSNDGDVPLGLTGDMAIFSI
RKTVPIPDGGCLLINNPAFSVNRPRRVRPNLFATYFVVSEMLARGSTTEPKRVNQMAYSCNFAFARLARMGLRGIHKIFQ
DHGDYLVYPSGNHFNEQVKHWMMSDVSSAILRGTEFQRIKERRRANFLHLLDKLIMNDRITLPILSLPEGVSPLFFPVIV
QDRDRVYRQLKDQGLSGHDWWGDFHPAVPWDKYPDAVYLKTNVFGFPVHQDLSRESLGIMLAEFSQCL

Sequences:

>Translated_388_residues
MKVSSKPSLTWEILTSRADRPRQEVFPFNTGRCEYFYSARYALAAALDALKLSPQQSILMPSYNCWVEVEPAVRSGAKID
WYRVKTDFSIDEDDLLARIRPETAAIFVIHYLGFPQRLENIHRICTERGIVLIEDCAHALLSNDGDVPLGLTGDMAIFSI
RKTVPIPDGGCLLINNPAFSVNRPRRVRPNLFATYFVVSEMLARGSTTEPKRVNQMAYSCNFAFARLARMGLRGIHKIFQ
DHGDYLVYPSGNHFNEQVKHWMMSDVSSAILRGTEFQRIKERRRANFLHLLDKLIMNDRITLPILSLPEGVSPLFFPVIV
QDRDRVYRQLKDQGLSGHDWWGDFHPAVPWDKYPDAVYLKTNVFGFPVHQDLSRESLGIMLAEFSQCL
>Mature_388_residues
MKVSSKPSLTWEILTSRADRPRQEVFPFNTGRCEYFYSARYALAAALDALKLSPQQSILMPSYNCWVEVEPAVRSGAKID
WYRVKTDFSIDEDDLLARIRPETAAIFVIHYLGFPQRLENIHRICTERGIVLIEDCAHALLSNDGDVPLGLTGDMAIFSI
RKTVPIPDGGCLLINNPAFSVNRPRRVRPNLFATYFVVSEMLARGSTTEPKRVNQMAYSCNFAFARLARMGLRGIHKIFQ
DHGDYLVYPSGNHFNEQVKHWMMSDVSSAILRGTEFQRIKERRRANFLHLLDKLIMNDRITLPILSLPEGVSPLFFPVIV
QDRDRVYRQLKDQGLSGHDWWGDFHPAVPWDKYPDAVYLKTNVFGFPVHQDLSRESLGIMLAEFSQCL

Specific function: Involved In Eca Elongation. [C]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 44429; Mature: 44429

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSSKPSLTWEILTSRADRPRQEVFPFNTGRCEYFYSARYALAAALDALKLSPQQSILM
CCCCCCCCEEHHHHHCCCCCCHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCEEC
PSYNCWVEVEPAVRSGAKIDWYRVKTDFSIDEDDLLARIRPETAAIFVIHYLGFPQRLEN
CCCCCEEEECHHHHCCCEEEEEEEEECCCCCHHHHHHHCCCCHHEEEEEHHCCCHHHHHH
IHRICTERGIVLIEDCAHALLSNDGDVPLGLTGDMAIFSIRKTVPIPDGGCLLINNPAFS
HHHHHHHCCCEEEHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEECCCCCC
VNRPRRVRPNLFATYFVVSEMLARGSTTEPKRVNQMAYSCNFAFARLARMGLRGIHKIFQ
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DHGDYLVYPSGNHFNEQVKHWMMSDVSSAILRGTEFQRIKERRRANFLHLLDKLIMNDRI
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
TLPILSLPEGVSPLFFPVIVQDRDRVYRQLKDQGLSGHDWWGDFHPAVPWDKYPDAVYLK
EEEEECCCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEE
TNVFGFPVHQDLSRESLGIMLAEFSQCL
ECEEECCCHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVSSKPSLTWEILTSRADRPRQEVFPFNTGRCEYFYSARYALAAALDALKLSPQQSILM
CCCCCCCCEEHHHHHCCCCCCHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCEEC
PSYNCWVEVEPAVRSGAKIDWYRVKTDFSIDEDDLLARIRPETAAIFVIHYLGFPQRLEN
CCCCCEEEECHHHHCCCEEEEEEEEECCCCCHHHHHHHCCCCHHEEEEEHHCCCHHHHHH
IHRICTERGIVLIEDCAHALLSNDGDVPLGLTGDMAIFSIRKTVPIPDGGCLLINNPAFS
HHHHHHHCCCEEEHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEECCCCCC
VNRPRRVRPNLFATYFVVSEMLARGSTTEPKRVNQMAYSCNFAFARLARMGLRGIHKIFQ
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DHGDYLVYPSGNHFNEQVKHWMMSDVSSAILRGTEFQRIKERRRANFLHLLDKLIMNDRI
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
TLPILSLPEGVSPLFFPVIVQDRDRVYRQLKDQGLSGHDWWGDFHPAVPWDKYPDAVYLK
EEEEECCCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEE
TNVFGFPVHQDLSRESLGIMLAEFSQCL
ECEEECCCHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose; L-glutamate [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = TDP-D-fucosamine + 2-oxoglutarate [C]

General reaction: Carbon-Oxygen Lyases; Hydro-Lyases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA