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The map label for this gene is ygcA [C]

Identifier: 78223178

GI number: 78223178

Start: 2201109

End: 2202590

Strand: Direct

Name: ygcA [C]

Synonym: Gmet_1971

Alternate gene names: 78223178

Gene position: 2201109-2202590 (Clockwise)

Preceding gene: 78223177

Following gene: 78223182

Centisome position: 55.06

GC content: 58.23

Gene sequence:

>1482_bases
ATGACTACGAGATACAGGAAACGAGAAGATAATAGAAACCATCGGACCGCCACCGGCCAAACGGTAACACTCACCATCAC
CGGTTTTGACGATGAAGGATTCGGAACGACATCCCACGAAGGGAAAAAAGTCCTCGTGGCAGGTGCCCTCCCTGGTGAAA
CGGTCAGCATCAGGGTAACTCACAACGGACCACGGACCATGTTCGGGGAGGCAATCACCATACTCTCCCCTTCCCCCGAC
CGCATGGCGTCTCCTTCATGCACCACCTCCGCCTGCAGCAACTGTCCTCTCATATCCATGAACTATGGGGCACAGCTTAC
CCTGAAACGCAGCCTCGTCGAAAAGGAGATCCGCTCCTTCGCCTCGCTGCGCGCAGTCCCCATAGACGATGTCATTTCCT
CTCCCCGCCCCCTACAGTACCGAAACTCCGCAAAGCTTGTTGTGGCGGGAACCTTCAAATCTCCAATCATCGGCATCTAT
CGGCGCAACAGCCACAACGTGGTCGATATCAGTGCCTGCCCCCTTCACAACCCACTCATCAATCGGGTGGTGGCTGCCGC
GAAGGAAGGAATACGAAAGGGGAAGGTGCCGGTTTACAATCCCCGCACCGACACCGGCATCCTCCGCTACCTTGTGGTGA
GGGTTTCCGAACACACTGGCCGGGCCATGGCCGTCTTCGTCACTGCCCAGAGAAGTTACAATGAGATTCACCATCTCGCC
AAGTTCGTAAGGCAGGCGGTCCCGGAACTGGATGTGGCGGTTCAGAACGTGAACACCTCCGAAGGGAATGTCATCCTCGC
CCCCCATGACCACTTCCTGACCAAAGACCATGCCATCATTGAAGAGCTTGCCGGGATCCGTTTTTCCATCTCGCCCCGCT
CGTTTTTCCAGGTGAACAGCGGCAGCGCCCGCATCATCTACGAAAAGGTCCGGGAATGGAGCGCCTTGACCGGCAATGAG
AAGGTCGTTGATCTCTATTGCGGCATCGGGGGGATTTCACTCTTCCTGGCCCCCGGCGCACAGGAGGTTCACGGCATAGA
GTACGTGGATGCGGCGGTAGCCGATGCGGAACGAAACGCGCGCCTCAACGGGATTCGCAACTGTTCCTTCGAGGCGGGTG
ATGCGGCAGAACTTCTGGAAGAGTTGGTGGAAGAAGGGACTGAACCCGACGTGGTCATCTTTAACCCGCCACGCAAGGGG
TGCGATCGCCGGGTGCTTGAGGCTGCCGTTAACTCAGGCCCTGGCCGGATGATCTATGTCTCCTGCTCCCCCGCCACCCT
GGCCCGCGACCTCGACATCCTCGCGGGGCTCGGCTACCAGACACGGGAGATCCAGCCCGTGGACATGTTCCCCCAGACTC
CTCACATTGAAAATGTGGCTCTTCTCGTCGATGCCCGTCACGCGCGGGATGACCACGGAAAAACAACGTCCCCCCAAGGT
CGGCAACATTTCACGCACAAAAGAAGAGGGGCATCCGCCTGA

Upstream 100 bases:

>100_bases
TCTGGAAGGTCTGAGCTTTGGCCTGCGGCTTGATGTCAGGACCGCCCACAGGTTTAATAAGTTCCTTGACAGGGAAGTGC
GACTCATTGTTAATAGGGAA

Downstream 100 bases:

>100_bases
ACGGGTGCCCCTCTCTCCACCTGACTATCGTGCCTGGTAACTATTTTCTCAGGACCTTGACCACTGAGGAAAAGTCCTCT
TCGCCGCGGCCATCCTTGAC

Product: 23S rRNA methyltransferase/RumA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MTTRYRKREDNRNHRTATGQTVTLTITGFDDEGFGTTSHEGKKVLVAGALPGETVSIRVTHNGPRTMFGEAITILSPSPD
RMASPSCTTSACSNCPLISMNYGAQLTLKRSLVEKEIRSFASLRAVPIDDVISSPRPLQYRNSAKLVVAGTFKSPIIGIY
RRNSHNVVDISACPLHNPLINRVVAAAKEGIRKGKVPVYNPRTDTGILRYLVVRVSEHTGRAMAVFVTAQRSYNEIHHLA
KFVRQAVPELDVAVQNVNTSEGNVILAPHDHFLTKDHAIIEELAGIRFSISPRSFFQVNSGSARIIYEKVREWSALTGNE
KVVDLYCGIGGISLFLAPGAQEVHGIEYVDAAVADAERNARLNGIRNCSFEAGDAAELLEELVEEGTEPDVVIFNPPRKG
CDRRVLEAAVNSGPGRMIYVSCSPATLARDLDILAGLGYQTREIQPVDMFPQTPHIENVALLVDARHARDDHGKTTSPQG
RQHFTHKRRGASA

Sequences:

>Translated_493_residues
MTTRYRKREDNRNHRTATGQTVTLTITGFDDEGFGTTSHEGKKVLVAGALPGETVSIRVTHNGPRTMFGEAITILSPSPD
RMASPSCTTSACSNCPLISMNYGAQLTLKRSLVEKEIRSFASLRAVPIDDVISSPRPLQYRNSAKLVVAGTFKSPIIGIY
RRNSHNVVDISACPLHNPLINRVVAAAKEGIRKGKVPVYNPRTDTGILRYLVVRVSEHTGRAMAVFVTAQRSYNEIHHLA
KFVRQAVPELDVAVQNVNTSEGNVILAPHDHFLTKDHAIIEELAGIRFSISPRSFFQVNSGSARIIYEKVREWSALTGNE
KVVDLYCGIGGISLFLAPGAQEVHGIEYVDAAVADAERNARLNGIRNCSFEAGDAAELLEELVEEGTEPDVVIFNPPRKG
CDRRVLEAAVNSGPGRMIYVSCSPATLARDLDILAGLGYQTREIQPVDMFPQTPHIENVALLVDARHARDDHGKTTSPQG
RQHFTHKRRGASA
>Mature_492_residues
TTRYRKREDNRNHRTATGQTVTLTITGFDDEGFGTTSHEGKKVLVAGALPGETVSIRVTHNGPRTMFGEAITILSPSPDR
MASPSCTTSACSNCPLISMNYGAQLTLKRSLVEKEIRSFASLRAVPIDDVISSPRPLQYRNSAKLVVAGTFKSPIIGIYR
RNSHNVVDISACPLHNPLINRVVAAAKEGIRKGKVPVYNPRTDTGILRYLVVRVSEHTGRAMAVFVTAQRSYNEIHHLAK
FVRQAVPELDVAVQNVNTSEGNVILAPHDHFLTKDHAIIEELAGIRFSISPRSFFQVNSGSARIIYEKVREWSALTGNEK
VVDLYCGIGGISLFLAPGAQEVHGIEYVDAAVADAERNARLNGIRNCSFEAGDAAELLEELVEEGTEPDVVIFNPPRKGC
DRRVLEAAVNSGPGRMIYVSCSPATLARDLDILAGLGYQTREIQPVDMFPQTPHIENVALLVDARHARDDHGKTTSPQGR
QHFTHKRRGASA

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=471, Percent_Identity=28.8747346072187, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI51173878, Length=471, Percent_Identity=28.8747346072187, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI269784680, Length=417, Percent_Identity=28.0575539568345, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI269784676, Length=417, Percent_Identity=28.0575539568345, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI20149688, Length=417, Percent_Identity=28.0575539568345, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI269784678, Length=285, Percent_Identity=29.8245614035088, Blast_Score=112, Evalue=7e-25,
Organism=Escherichia coli, GI1789148, Length=462, Percent_Identity=28.3549783549784, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1787083, Length=378, Percent_Identity=29.1005291005291, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI1790403, Length=380, Percent_Identity=23.9473684210526, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322909, Length=401, Percent_Identity=25.4364089775561, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24666579, Length=437, Percent_Identity=28.8329519450801, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 53972; Mature: 53841

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRYRKREDNRNHRTATGQTVTLTITGFDDEGFGTTSHEGKKVLVAGALPGETVSIRVT
CCCCCCCCCCCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEE
HNGPRTMFGEAITILSPSPDRMASPSCTTSACSNCPLISMNYGAQLTLKRSLVEKEIRSF
ECCCCCCCCCEEEEECCCCCCCCCCCCCHHHHCCCCEEEECCCCEEEHHHHHHHHHHHHH
ASLRAVPIDDVISSPRPLQYRNSAKLVVAGTFKSPIIGIYRRNSHNVVDISACPLHNPLI
HHCCCCCHHHHHCCCCCCEECCCCCEEEEECCCCCEEEEEECCCCCEEEEECCCCCCHHH
NRVVAAAKEGIRKGKVPVYNPRTDTGILRYLVVRVSEHTGRAMAVFVTAQRSYNEIHHLA
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHH
KFVRQAVPELDVAVQNVNTSEGNVILAPHDHFLTKDHAIIEELAGIRFSISPRSFFQVNS
HHHHHHCCCCEEEEEECCCCCCCEEECCCCCCCCCHHHHHHHHCCCEEEECCCCEEEECC
GSARIIYEKVREWSALTGNEKVVDLYCGIGGISLFLAPGAQEVHGIEYVDAAVADAERNA
CCEEEHHHHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCHHHCCHHHHHHHHHCCCCCC
RLNGIRNCSFEAGDAAELLEELVEEGTEPDVVIFNPPRKGCDRRVLEAAVNSGPGRMIYV
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCEEEEE
SCSPATLARDLDILAGLGYQTREIQPVDMFPQTPHIENVALLVDARHARDDHGKTTSPQG
ECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
RQHFTHKRRGASA
HHHHHHHHCCCCC
>Mature Secondary Structure 
TTRYRKREDNRNHRTATGQTVTLTITGFDDEGFGTTSHEGKKVLVAGALPGETVSIRVT
CCCCCCCCCCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEE
HNGPRTMFGEAITILSPSPDRMASPSCTTSACSNCPLISMNYGAQLTLKRSLVEKEIRSF
ECCCCCCCCCEEEEECCCCCCCCCCCCCHHHHCCCCEEEECCCCEEEHHHHHHHHHHHHH
ASLRAVPIDDVISSPRPLQYRNSAKLVVAGTFKSPIIGIYRRNSHNVVDISACPLHNPLI
HHCCCCCHHHHHCCCCCCEECCCCCEEEEECCCCCEEEEEECCCCCEEEEECCCCCCHHH
NRVVAAAKEGIRKGKVPVYNPRTDTGILRYLVVRVSEHTGRAMAVFVTAQRSYNEIHHLA
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHH
KFVRQAVPELDVAVQNVNTSEGNVILAPHDHFLTKDHAIIEELAGIRFSISPRSFFQVNS
HHHHHHCCCCEEEEEECCCCCCCEEECCCCCCCCCHHHHHHHHCCCEEEECCCCEEEECC
GSARIIYEKVREWSALTGNEKVVDLYCGIGGISLFLAPGAQEVHGIEYVDAAVADAERNA
CCEEEHHHHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCHHHCCHHHHHHHHHCCCCCC
RLNGIRNCSFEAGDAAELLEELVEEGTEPDVVIFNPPRKGCDRRVLEAAVNSGPGRMIYV
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCEEEEE
SCSPATLARDLDILAGLGYQTREIQPVDMFPQTPHIENVALLVDARHARDDHGKTTSPQG
ECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
RQHFTHKRRGASA
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA