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The map label for this gene is tpiA

Identifier: 78223155

GI number: 78223155

Start: 2176614

End: 2177369

Strand: Direct

Name: tpiA

Synonym: Gmet_1948

Alternate gene names: 78223155

Gene position: 2176614-2177369 (Clockwise)

Preceding gene: 78223154

Following gene: 78223156

Centisome position: 54.45

GC content: 50.66

Gene sequence:

>756_bases
ATGAGAACACCGATTATTGCTGGCAATTGGAAACTCTTCAAAAAGAGCAGCGAAGCACAAGACTTCGTTGCCGAACTGAT
CCCTCTCGTCCAGAAAACCACTGACGTGGAAATCGTTATTGCGCCTGTATTTACCGTTCTTTCTGCCGTTAAACGAGCCA
TTGCCGATTGCAACATAATGCTTTCTGCTCAAGATTGCTTCTGGGAAGAGGAAGGGGCCTTCACCGGCGAAATTTCTCCC
GGCATGCTTGTGGACGCCGGATGCAGCCATGTTATAATTGGCCATTCCGAGCGGAGGCAGTATTTTGGTGAAACCGACGA
AACCGTCAACCGGAAGATCAAGGCGGCTATTACAGCAGGTCTTACAGTCCTTTTCTGCATCGGTGAAACACTTGCCGAAC
GTGAAGCAGACAAAACCTTTGAGGTGCTCAGGACCCAGATCGAGAATGGTTTGGCCGGACTCGCAAGGGGCGATCTTGCC
AAAATCGTAATTGCGTACGAACCGGTCTGGGCCATAGGTACCGGTAAAACCGCCACTGATGAGCAGGCACAGATAGCGCA
TGCATTCATCCGCAAGGTCGTGGGCGAGCTGTTTACCGTCCAGATAGCCGAAACTATTCGTATACTCTATGGCGGAAGCG
TAAAACCGGAAAATGTCAGAGGGCTCATGAATCAACCTGACATCGACGGCGCTTTGGTGGGTGGAGCGAGCCTCAAGGCA
GACTCATTTGGAGCAATCGTCAACTATAAAGCGTAA

Upstream 100 bases:

>100_bases
CAGGCGGCGGCGCGTTCCTGGAACTCCTCGAAGGGAAGCATCTTCCCGGCATCAAAGTGCTGGAAGAAAACGGCAAGTAG
ATAACAAACGGGGTGACGAC

Downstream 100 bases:

>100_bases
AACGCCACGGTTGATTCTCTATTGGCATTTCCTGAATGATGTGCTATTAGTTTTAAGTTGCTACATTCATTGGAGTTACG
TCTTATGACTATTCTACTCA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MRTPIIAGNWKLFKKSSEAQDFVAELIPLVQKTTDVEIVIAPVFTVLSAVKRAIADCNIMLSAQDCFWEEEGAFTGEISP
GMLVDAGCSHVIIGHSERRQYFGETDETVNRKIKAAITAGLTVLFCIGETLAEREADKTFEVLRTQIENGLAGLARGDLA
KIVIAYEPVWAIGTGKTATDEQAQIAHAFIRKVVGELFTVQIAETIRILYGGSVKPENVRGLMNQPDIDGALVGGASLKA
DSFGAIVNYKA

Sequences:

>Translated_251_residues
MRTPIIAGNWKLFKKSSEAQDFVAELIPLVQKTTDVEIVIAPVFTVLSAVKRAIADCNIMLSAQDCFWEEEGAFTGEISP
GMLVDAGCSHVIIGHSERRQYFGETDETVNRKIKAAITAGLTVLFCIGETLAEREADKTFEVLRTQIENGLAGLARGDLA
KIVIAYEPVWAIGTGKTATDEQAQIAHAFIRKVVGELFTVQIAETIRILYGGSVKPENVRGLMNQPDIDGALVGGASLKA
DSFGAIVNYKA
>Mature_251_residues
MRTPIIAGNWKLFKKSSEAQDFVAELIPLVQKTTDVEIVIAPVFTVLSAVKRAIADCNIMLSAQDCFWEEEGAFTGEISP
GMLVDAGCSHVIIGHSERRQYFGETDETVNRKIKAAITAGLTVLFCIGETLAEREADKTFEVLRTQIENGLAGLARGDLA
KIVIAYEPVWAIGTGKTATDEQAQIAHAFIRKVVGELFTVQIAETIRILYGGSVKPENVRGLMNQPDIDGALVGGASLKA
DSFGAIVNYKA

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=249, Percent_Identity=42.9718875502008, Blast_Score=191, Evalue=5e-49,
Organism=Homo sapiens, GI226529917, Length=249, Percent_Identity=42.9718875502008, Blast_Score=191, Evalue=5e-49,
Organism=Escherichia coli, GI1790353, Length=247, Percent_Identity=47.3684210526316, Blast_Score=207, Evalue=7e-55,
Organism=Caenorhabditis elegans, GI17536593, Length=254, Percent_Identity=43.3070866141732, Blast_Score=195, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6320255, Length=249, Percent_Identity=40.9638554216867, Blast_Score=172, Evalue=6e-44,
Organism=Drosophila melanogaster, GI28572008, Length=255, Percent_Identity=43.5294117647059, Blast_Score=190, Evalue=8e-49,
Organism=Drosophila melanogaster, GI28572006, Length=255, Percent_Identity=43.5294117647059, Blast_Score=190, Evalue=8e-49,
Organism=Drosophila melanogaster, GI28572004, Length=255, Percent_Identity=43.5294117647059, Blast_Score=189, Evalue=1e-48,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_GEOMG (Q39U97)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384902.1
- HSSP:   P36204
- ProteinModelPortal:   Q39U97
- SMR:   Q39U97
- STRING:   Q39U97
- GeneID:   3741599
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1948
- NMPDR:   fig|269799.3.peg.840
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- PhylomeDB:   Q39U97
- ProtClustDB:   PRK00042
- BioCyc:   GMET269799:GMET_1948-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 27116; Mature: 27116

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 95-95 ACT_SITE 167-167 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTPIIAGNWKLFKKSSEAQDFVAELIPLVQKTTDVEIVIAPVFTVLSAVKRAIADCNIM
CCCCEEECCCCEECCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCEEE
LSAQDCFWEEEGAFTGEISPGMLVDAGCSHVIIGHSERRQYFGETDETVNRKIKAAITAG
EEHHHHCCCCCCCEECCCCCCEEEECCCCEEEECCHHHHHHCCCCHHHHHHHHHHHHHHH
LTVLFCIGETLAEREADKTFEVLRTQIENGLAGLARGDLAKIVIAYEPVWAIGTGKTATD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCEEEEEEECCEEEEECCCCCCC
EQAQIAHAFIRKVVGELFTVQIAETIRILYGGSVKPENVRGLMNQPDIDGALVGGASLKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCEEEECCCCCCC
DSFGAIVNYKA
CCCCCEEEECC
>Mature Secondary Structure
MRTPIIAGNWKLFKKSSEAQDFVAELIPLVQKTTDVEIVIAPVFTVLSAVKRAIADCNIM
CCCCEEECCCCEECCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCEEE
LSAQDCFWEEEGAFTGEISPGMLVDAGCSHVIIGHSERRQYFGETDETVNRKIKAAITAG
EEHHHHCCCCCCCEECCCCCCEEEECCCCEEEECCHHHHHHCCCCHHHHHHHHHHHHHHH
LTVLFCIGETLAEREADKTFEVLRTQIENGLAGLARGDLAKIVIAYEPVWAIGTGKTATD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCEEEEEEECCEEEEECCCCCCC
EQAQIAHAFIRKVVGELFTVQIAETIRILYGGSVKPENVRGLMNQPDIDGALVGGASLKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCEEEECCCCCCC
DSFGAIVNYKA
CCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA