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The map label for this gene is purB [H]

Identifier: 78223150

GI number: 78223150

Start: 2169723

End: 2171018

Strand: Reverse

Name: purB [H]

Synonym: Gmet_1943

Alternate gene names: 78223150

Gene position: 2171018-2169723 (Counterclockwise)

Preceding gene: 78223151

Following gene: 78223149

Centisome position: 54.31

GC content: 59.18

Gene sequence:

>1296_bases
GTGATCGAACGCTACAGTCGTCCTGAAATGACCCGCATCTGGGAAGCGCGCAACCGTTATCAGAAATGGCTCGACATAGA
AATCTTCGCTTGTGAGGCCCATGCCGAACTGGGCAATATCCCCCGTGAGGCCGTCGAGCGGATCAAGGCCAAGGCCGACT
TCGACGTTGAGCGGATCGACGAGATCGAGCGAACCGTCAAGCACGACGTCATTGCTTTCCTCACCTCCGTCGCCGACTAC
ATCGGCGACGATTCCCGCTTCGTGCACCTGGGTCTTACCTCCTCCGACGTTCTCGATACCTCCTTTGCCATGCTCCTGGC
CGAGGCTTCCGACCTCATCATTGATGACATCAAGCGCCTCATGGAGGTGATCAAGCGCCGGGCCTTCGAGCACAGGGATA
CGCCCATGATGGGACGTTCCCACGGCATCCACGCCGAACCGGTTACCTTCGGCATCAAGATGGCGCTCTGGTACGACGAG
ATGCGGCGCAACCTCCGCCGGATGGAGTCTGCCCGCGAAACCATCGCCTACGGAAAGATTTCCGGTGCGGTCGGTACCTT
TGCCAATATTGATCCGCGGGTAGAGGAATACGTCTGCAAGAAGGGAGGCCTCAAGCCTGCTCCCTGCTCGACCCAGGTGA
TCCAGCGGGACCGCCATGCCGAGTTCTTCTCGGCCCTAGCCATCATTGCCAGCTCCATCGAAAAATTCGCTGTTGAGATC
CGCCATCTCCAGCGTACCGAAGTCCTGGAGGCGGAGGAATACTTCAGCAAGGGGCAGAAAGGCTCCTCGGCAATGCCCCA
CAAGCGCAACCCCGTCCTTTCCGAGAACCTCACCGGCCTGGCCCGCCTCATACGGGGCTACGCGGTTTCGGCCATGGAGA
ACGTGCCGCTCTGGCACGAGAGAGACATCTCCCACTCGTCGGTGGAGCGGGTCATCGGTCCCGATGCCACCATCGTCATG
GATTTCATGCTCAACCGCTGCATCGGCCTCATCGACAACCTTGTCGTCTACCCCGAGAACATGATGCGTAACCTCAACCT
CATGCGGGGGCTCATCTTCTCCCAGCGGGTGCTTCTGAAGTTGGCCAATGCGGGCGCATCCCGCGAGAAGGCCTATGAGC
TTGTCCAGCGCAACGCCATGAAGGTCTGGGAGCAGGGGAAGGATTTCCAGGAAGAACTTCTGGCCGATGCCGACGTGCGT
GGCTTTCTTCCCGAGGAGGAAATCCGCGAGGCCTTTGACCTCAACTATCACCTGAAGCATGTGGGCACCATCTTCACGAG
GGTCTTTGGTGGATAA

Upstream 100 bases:

>100_bases
CGTCCCAGTGAAACGCTGACCCTCTGACAACACTGTCGAGGGCTGGCACTCTATCGAGCGCCGGCCCTGTTATTTTGCCA
AAGACGAAGGAGAATGCACC

Downstream 100 bases:

>100_bases
CATCCTCAATTTCCTCCGGCTCGAGGAGTCAGATCACCTCCTTCTCTTCTGGTTGAAGGAGATCCTCGTAGCCGTTGTCA
TATTCACGTTATTTTGGGGT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIERYSRPEMTRIWEARNRYQKWLDIEIFACEAHAELGNIPREAVERIKAKADFDVERIDEIERTVKHDVIAFLTSVADY
IGDDSRFVHLGLTSSDVLDTSFAMLLAEASDLIIDDIKRLMEVIKRRAFEHRDTPMMGRSHGIHAEPVTFGIKMALWYDE
MRRNLRRMESARETIAYGKISGAVGTFANIDPRVEEYVCKKGGLKPAPCSTQVIQRDRHAEFFSALAIIASSIEKFAVEI
RHLQRTEVLEAEEYFSKGQKGSSAMPHKRNPVLSENLTGLARLIRGYAVSAMENVPLWHERDISHSSVERVIGPDATIVM
DFMLNRCIGLIDNLVVYPENMMRNLNLMRGLIFSQRVLLKLANAGASREKAYELVQRNAMKVWEQGKDFQEELLADADVR
GFLPEEEIREAFDLNYHLKHVGTIFTRVFGG

Sequences:

>Translated_431_residues
MIERYSRPEMTRIWEARNRYQKWLDIEIFACEAHAELGNIPREAVERIKAKADFDVERIDEIERTVKHDVIAFLTSVADY
IGDDSRFVHLGLTSSDVLDTSFAMLLAEASDLIIDDIKRLMEVIKRRAFEHRDTPMMGRSHGIHAEPVTFGIKMALWYDE
MRRNLRRMESARETIAYGKISGAVGTFANIDPRVEEYVCKKGGLKPAPCSTQVIQRDRHAEFFSALAIIASSIEKFAVEI
RHLQRTEVLEAEEYFSKGQKGSSAMPHKRNPVLSENLTGLARLIRGYAVSAMENVPLWHERDISHSSVERVIGPDATIVM
DFMLNRCIGLIDNLVVYPENMMRNLNLMRGLIFSQRVLLKLANAGASREKAYELVQRNAMKVWEQGKDFQEELLADADVR
GFLPEEEIREAFDLNYHLKHVGTIFTRVFGG
>Mature_431_residues
MIERYSRPEMTRIWEARNRYQKWLDIEIFACEAHAELGNIPREAVERIKAKADFDVERIDEIERTVKHDVIAFLTSVADY
IGDDSRFVHLGLTSSDVLDTSFAMLLAEASDLIIDDIKRLMEVIKRRAFEHRDTPMMGRSHGIHAEPVTFGIKMALWYDE
MRRNLRRMESARETIAYGKISGAVGTFANIDPRVEEYVCKKGGLKPAPCSTQVIQRDRHAEFFSALAIIASSIEKFAVEI
RHLQRTEVLEAEEYFSKGQKGSSAMPHKRNPVLSENLTGLARLIRGYAVSAMENVPLWHERDISHSSVERVIGPDATIVM
DFMLNRCIGLIDNLVVYPENMMRNLNLMRGLIFSQRVLLKLANAGASREKAYELVQRNAMKVWEQGKDFQEELLADADVR
GFLPEEEIREAFDLNYHLKHVGTIFTRVFGG

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=406, Percent_Identity=28.5714285714286, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI183227688, Length=383, Percent_Identity=28.4595300261097, Blast_Score=159, Evalue=3e-39,
Organism=Escherichia coli, GI1787376, Length=312, Percent_Identity=30.4487179487179, Blast_Score=109, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17508577, Length=400, Percent_Identity=27.5, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI32564234, Length=344, Percent_Identity=29.6511627906977, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6323391, Length=407, Percent_Identity=28.2555282555283, Blast_Score=163, Evalue=6e-41,
Organism=Drosophila melanogaster, GI24647570, Length=403, Percent_Identity=27.0471464019851, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49350; Mature: 49350

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYSRPEMTRIWEARNRYQKWLDIEIFACEAHAELGNIPREAVERIKAKADFDVERID
CCCCCCCCHHHHHHHHHHHHHHHHCEEEEEEECHHHHCCCCHHHHHHHHHHCCCCHHHHH
EIERTVKHDVIAFLTSVADYIGDDSRFVHLGLTSSDVLDTSFAMLLAEASDLIIDDIKRL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MEVIKRRAFEHRDTPMMGRSHGIHAEPVTFGIKMALWYDEMRRNLRRMESARETIAYGKI
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTFANIDPRVEEYVCKKGGLKPAPCSTQVIQRDRHAEFFSALAIIASSIEKFAVEI
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLQRTEVLEAEEYFSKGQKGSSAMPHKRNPVLSENLTGLARLIRGYAVSAMENVPLWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RDISHSSVERVIGPDATIVMDFMLNRCIGLIDNLVVYPENMMRNLNLMRGLIFSQRVLLK
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LANAGASREKAYELVQRNAMKVWEQGKDFQEELLADADVRGFLPEEEIREAFDLNYHLKH
HHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHCCCCHHHHHHHHCCCHHHHH
VGTIFTRVFGG
HHHHHHHHHCC
>Mature Secondary Structure
MIERYSRPEMTRIWEARNRYQKWLDIEIFACEAHAELGNIPREAVERIKAKADFDVERID
CCCCCCCCHHHHHHHHHHHHHHHHCEEEEEEECHHHHCCCCHHHHHHHHHHCCCCHHHHH
EIERTVKHDVIAFLTSVADYIGDDSRFVHLGLTSSDVLDTSFAMLLAEASDLIIDDIKRL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MEVIKRRAFEHRDTPMMGRSHGIHAEPVTFGIKMALWYDEMRRNLRRMESARETIAYGKI
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTFANIDPRVEEYVCKKGGLKPAPCSTQVIQRDRHAEFFSALAIIASSIEKFAVEI
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLQRTEVLEAEEYFSKGQKGSSAMPHKRNPVLSENLTGLARLIRGYAVSAMENVPLWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RDISHSSVERVIGPDATIVMDFMLNRCIGLIDNLVVYPENMMRNLNLMRGLIFSQRVLLK
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LANAGASREKAYELVQRNAMKVWEQGKDFQEELLADADVRGFLPEEEIREAFDLNYHLKH
HHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHCCCCHHHHHHHHCCCHHHHH
VGTIFTRVFGG
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]