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The map label for this gene is kefA [C]

Identifier: 78223149

GI number: 78223149

Start: 2168648

End: 2169730

Strand: Reverse

Name: kefA [C]

Synonym: Gmet_1942

Alternate gene names: 78223149

Gene position: 2169730-2168648 (Counterclockwise)

Preceding gene: 78223150

Following gene: 78223148

Centisome position: 54.28

GC content: 57.53

Gene sequence:

>1083_bases
GTGGATAACATCCTCAATTTCCTCCGGCTCGAGGAGTCAGATCACCTCCTTCTCTTCTGGTTGAAGGAGATCCTCGTAGC
CGTTGTCATATTCACGTTATTTTGGGGTCTTTCCCAGGTGGTTCGCTACATCCTCACCTCCTGGGTGCCCCGGTTGACCG
CCTTTACGCGCACCGGCCTCGACGACCGGATCATCCAGCGGGTAACGCCGCCGGCAAGCCTCCTGGTGGTTTTCGCCGGC
CTTTACTTTGCGGTACGGTCACTGCCGCTCCCCGAGAAGGCCCATGTGGTTTTGTCGGGAGCGGTATATATCCTCAACAT
TATCGTCGTCACCGTTATCGTGGAGCGGGTCATTGCGGAGCTCCTCGAATGGTACGGTGCGAAGCTCGCCGAGCGGCACG
GTGCCGGAGTTGACCGGCAGATCATCCCCCCCCTGGAAAAGATCGTGACGATTTTTCTGGTGGGAATTGCCCTGATGGTG
ACGCTCAGGCACTTCGACTATGACATCGTGTCGGTGCTTACGGCTCTTGGCGTCGGCTCCCTCGCCATCGGCATGGCGGC
CAAGGACACCCTGGCCAACATGATTTCCGGCTTTACCCTCATGGTTGACCGGCCGTTCCGGATCGGAGACCGGATCCAAC
TCACCTCCGGCCAATGGGGAGATGTGAACGATATCGGCCTGCGCACCACCAAGATCAAGACCGTCGACAACACGCTTCTT
GTCATTCCCAACTCCGAGCTCTGCAATACGACGCTTATCAACCTGGCATATCCCGACACGAGGGCCAAGGGGCGGATAAA
CGTGGGGGTCGGCTACGAAAGTGACGTGGAAAAGGTTAAGGGGATCATGATTCAAACGGCCCTCGACGTCCCCGAGGTGC
TCCCCAATCCTGCTCCGGAGGCGTTTTTCGTTTCCTTTGGCGATAGTGCCCTCGGCATGTCGCTCTTCTTCTGGGTCGAG
GATTATACGCGGCTCTTCGCCGTTACCGACCGCATCAACGAACAGATCATCAACCGGTTCAGGGAGCATGGCATCACCAT
ACCCTACCCGACACGGACCGTGTTCCTGGAAAAGGAAAACTAA

Upstream 100 bases:

>100_bases
GCCGACGTGCGTGGCTTTCTTCCCGAGGAGGAAATCCGCGAGGCCTTTGACCTCAACTATCACCTGAAGCATGTGGGCAC
CATCTTCACGAGGGTCTTTG

Downstream 100 bases:

>100_bases
ATGGCGAGACGAATTGAGATTGCCCTCAAGGAAGGCGTCCGTGACGCCCGTGGCGAGCGGATCAAACGCGAGATCGAGCA
TTTTCTCCATCTGCCGGTGG

Product: MscS mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MDNILNFLRLEESDHLLLFWLKEILVAVVIFTLFWGLSQVVRYILTSWVPRLTAFTRTGLDDRIIQRVTPPASLLVVFAG
LYFAVRSLPLPEKAHVVLSGAVYILNIIVVTVIVERVIAELLEWYGAKLAERHGAGVDRQIIPPLEKIVTIFLVGIALMV
TLRHFDYDIVSVLTALGVGSLAIGMAAKDTLANMISGFTLMVDRPFRIGDRIQLTSGQWGDVNDIGLRTTKIKTVDNTLL
VIPNSELCNTTLINLAYPDTRAKGRINVGVGYESDVEKVKGIMIQTALDVPEVLPNPAPEAFFVSFGDSALGMSLFFWVE
DYTRLFAVTDRINEQIINRFREHGITIPYPTRTVFLEKEN

Sequences:

>Translated_360_residues
MDNILNFLRLEESDHLLLFWLKEILVAVVIFTLFWGLSQVVRYILTSWVPRLTAFTRTGLDDRIIQRVTPPASLLVVFAG
LYFAVRSLPLPEKAHVVLSGAVYILNIIVVTVIVERVIAELLEWYGAKLAERHGAGVDRQIIPPLEKIVTIFLVGIALMV
TLRHFDYDIVSVLTALGVGSLAIGMAAKDTLANMISGFTLMVDRPFRIGDRIQLTSGQWGDVNDIGLRTTKIKTVDNTLL
VIPNSELCNTTLINLAYPDTRAKGRINVGVGYESDVEKVKGIMIQTALDVPEVLPNPAPEAFFVSFGDSALGMSLFFWVE
DYTRLFAVTDRINEQIINRFREHGITIPYPTRTVFLEKEN
>Mature_360_residues
MDNILNFLRLEESDHLLLFWLKEILVAVVIFTLFWGLSQVVRYILTSWVPRLTAFTRTGLDDRIIQRVTPPASLLVVFAG
LYFAVRSLPLPEKAHVVLSGAVYILNIIVVTVIVERVIAELLEWYGAKLAERHGAGVDRQIIPPLEKIVTIFLVGIALMV
TLRHFDYDIVSVLTALGVGSLAIGMAAKDTLANMISGFTLMVDRPFRIGDRIQLTSGQWGDVNDIGLRTTKIKTVDNTLL
VIPNSELCNTTLINLAYPDTRAKGRINVGVGYESDVEKVKGIMIQTALDVPEVLPNPAPEAFFVSFGDSALGMSLFFWVE
DYTRLFAVTDRINEQIINRFREHGITIPYPTRTVFLEKEN

Specific function: Unknown

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=186, Percent_Identity=33.8709677419355, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1787591, Length=226, Percent_Identity=31.858407079646, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789291, Length=272, Percent_Identity=26.8382352941176, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI2367355, Length=189, Percent_Identity=27.5132275132275, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 40288; Mature: 40288

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNILNFLRLEESDHLLLFWLKEILVAVVIFTLFWGLSQVVRYILTSWVPRLTAFTRTGL
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DDRIIQRVTPPASLLVVFAGLYFAVRSLPLPEKAHVVLSGAVYILNIIVVTVIVERVIAE
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
LLEWYGAKLAERHGAGVDRQIIPPLEKIVTIFLVGIALMVTLRHFDYDIVSVLTALGVGS
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCH
LAIGMAAKDTLANMISGFTLMVDRPFRIGDRIQLTSGQWGDVNDIGLRTTKIKTVDNTLL
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCEEEEEEEECCEEE
VIPNSELCNTTLINLAYPDTRAKGRINVGVGYESDVEKVKGIMIQTALDVPEVLPNPAPE
EECCCCCCCCEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCC
AFFVSFGDSALGMSLFFWVEDYTRLFAVTDRINEQIINRFREHGITIPYPTRTVFLEKEN
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MDNILNFLRLEESDHLLLFWLKEILVAVVIFTLFWGLSQVVRYILTSWVPRLTAFTRTGL
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DDRIIQRVTPPASLLVVFAGLYFAVRSLPLPEKAHVVLSGAVYILNIIVVTVIVERVIAE
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
LLEWYGAKLAERHGAGVDRQIIPPLEKIVTIFLVGIALMVTLRHFDYDIVSVLTALGVGS
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCH
LAIGMAAKDTLANMISGFTLMVDRPFRIGDRIQLTSGQWGDVNDIGLRTTKIKTVDNTLL
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCEEEEEEEECCEEE
VIPNSELCNTTLINLAYPDTRAKGRINVGVGYESDVEKVKGIMIQTALDVPEVLPNPAPE
EECCCCCCCCEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCHHHCCCCCCC
AFFVSFGDSALGMSLFFWVEDYTRLFAVTDRINEQIINRFREHGITIPYPTRTVFLEKEN
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]