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The map label for this gene is rpfG [H]

Identifier: 78223126

GI number: 78223126

Start: 2145182

End: 2146234

Strand: Direct

Name: rpfG [H]

Synonym: Gmet_1919

Alternate gene names: 78223126

Gene position: 2145182-2146234 (Clockwise)

Preceding gene: 78223125

Following gene: 78223129

Centisome position: 53.66

GC content: 56.41

Gene sequence:

>1053_bases
GTGCTACCCACCATCTTGACTGTCGACGACGAGGAAATGATCAGAGACCTTATGGTTACGGCCCTTTCCCGTGAGGGATA
CCGTTGCTTTCAAGCCGGCAGCGCCGAGGAGGCTCGTTCCGTCCTCCAGAACAACAAGATTGATCTCGCGATTCTCGACA
TCATGATGCCCGGGCTTTCGGGCGATGACTTTCTCAAGGAACTCAAAGTACTGAGTCCCGAGACCGTGGTGCTTATGGTA
ACAGCCCTGAGTGACACGGAAACAGCCATGAAGTGCATCCACCTCGGGGCCTACGATTATATCCTCAAGCCCTTCGATAT
CGACCGGGTACTCCTTACTATCCACAACGCCCTGGAAAAACAGCGTCTCCTCGCCGAGAACCGGGAATACCAGGCCAATC
TGGAGAAAAAAGTCGACGAGCAGACCCGTCAGATTCGCATGGCCATGGAAGACCTGAACCTCGCCTACAATCATACCCTG
ACGGCTCTCGTGAGGGCGCTCGATGCCCGCGAGAAGGAAACTGGCTCCCACTCGGAGCGGGTTATGACTTACACCCTGCT
CCTCGGGGAGGCCATGGGCGTCCCTGAGAAGGATCTGAGTGTCATGGCGCGGGGAGCACTTTTGCACGACATCGGCAAGG
TCGGCATCTCCGACAATATACTCCTGAAACCTGCCAAGCTCGATGAACGCGAATGGATGGAGATGATGCGCCATCCCCAA
CTCGGCTACGAGATCCTCTCGGGAATCAAGTTTCTCAAGGGACCAGCCGAAATAGTATTTGCACACCATGAGCGGTTCGA
CGGCAGCGGTTATCCAAAGGGACTTTCCCGAAGCGGCATTCCCCTTGGCGCTCGCATCTTCTCCCTGGTGGATACCCTTG
ATGCCATGACGTCGGACAGACCTTACCGCAAAGCGCTCCCCTTCGAGGCGGTCACCAGCGAGGTGAGACGTTGCAGGGGA
ACCCAGTTCGATCCGGAGATCGTCGACATTTTCCTCTCGATTCCCCGTGAGCGGTGGGAAGTCGTCGGGAAGAGGCATTT
CATCGTTGGCTAG

Upstream 100 bases:

>100_bases
CAGCCTTTATCAACAAGCCTTTCACGCCCGATGTGCTGGTCGAACGTGTGCGGGATGTCCTTGAGGGCCGGCTGAATATG
TAAACGCGAGAGGAGCTCCT

Downstream 100 bases:

>100_bases
TCTTTCCATCTCTTATCAGCGCAGAAACAGTCAGACCTCCCCCCTGGCCACTATCGTTTTTCGGCCGTAAACGGGAAAGG
GCCGCCCAGAATGGACGGCC

Product: response regulator receiver (CheY-like) modulated metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MLPTILTVDDEEMIRDLMVTALSREGYRCFQAGSAEEARSVLQNNKIDLAILDIMMPGLSGDDFLKELKVLSPETVVLMV
TALSDTETAMKCIHLGAYDYILKPFDIDRVLLTIHNALEKQRLLAENREYQANLEKKVDEQTRQIRMAMEDLNLAYNHTL
TALVRALDAREKETGSHSERVMTYTLLLGEAMGVPEKDLSVMARGALLHDIGKVGISDNILLKPAKLDEREWMEMMRHPQ
LGYEILSGIKFLKGPAEIVFAHHERFDGSGYPKGLSRSGIPLGARIFSLVDTLDAMTSDRPYRKALPFEAVTSEVRRCRG
TQFDPEIVDIFLSIPRERWEVVGKRHFIVG

Sequences:

>Translated_350_residues
MLPTILTVDDEEMIRDLMVTALSREGYRCFQAGSAEEARSVLQNNKIDLAILDIMMPGLSGDDFLKELKVLSPETVVLMV
TALSDTETAMKCIHLGAYDYILKPFDIDRVLLTIHNALEKQRLLAENREYQANLEKKVDEQTRQIRMAMEDLNLAYNHTL
TALVRALDAREKETGSHSERVMTYTLLLGEAMGVPEKDLSVMARGALLHDIGKVGISDNILLKPAKLDEREWMEMMRHPQ
LGYEILSGIKFLKGPAEIVFAHHERFDGSGYPKGLSRSGIPLGARIFSLVDTLDAMTSDRPYRKALPFEAVTSEVRRCRG
TQFDPEIVDIFLSIPRERWEVVGKRHFIVG
>Mature_350_residues
MLPTILTVDDEEMIRDLMVTALSREGYRCFQAGSAEEARSVLQNNKIDLAILDIMMPGLSGDDFLKELKVLSPETVVLMV
TALSDTETAMKCIHLGAYDYILKPFDIDRVLLTIHNALEKQRLLAENREYQANLEKKVDEQTRQIRMAMEDLNLAYNHTL
TALVRALDAREKETGSHSERVMTYTLLLGEAMGVPEKDLSVMARGALLHDIGKVGISDNILLKPAKLDEREWMEMMRHPQ
LGYEILSGIKFLKGPAEIVFAHHERFDGSGYPKGLSRSGIPLGARIFSLVDTLDAMTSDRPYRKALPFEAVTSEVRRCRG
TQFDPEIVDIFLSIPRERWEVVGKRHFIVG

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=130, Percent_Identity=33.8461538461538, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790299, Length=129, Percent_Identity=32.5581395348837, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790437, Length=114, Percent_Identity=35.9649122807018, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1786599, Length=118, Percent_Identity=33.0508474576271, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789809, Length=117, Percent_Identity=31.6239316239316, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1786911, Length=122, Percent_Identity=32.7868852459016, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI1790863, Length=134, Percent_Identity=31.3432835820896, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 39562; Mature: 39562

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPTILTVDDEEMIRDLMVTALSREGYRCFQAGSAEEARSVLQNNKIDLAILDIMMPGLS
CCCCEEEECHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCCCEEEEEHHCCCCC
GDDFLKELKVLSPETVVLMVTALSDTETAMKCIHLGAYDYILKPFDIDRVLLTIHNALEK
CHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHH
QRLLAENREYQANLEKKVDEQTRQIRMAMEDLNLAYNHTLTALVRALDAREKETGSHSER
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
VMTYTLLLGEAMGVPEKDLSVMARGALLHDIGKVGISDNILLKPAKLDEREWMEMMRHPQ
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCC
LGYEILSGIKFLKGPAEIVFAHHERFDGSGYPKGLSRSGIPLGARIFSLVDTLDAMTSDR
CHHHHHHHHHHHCCCHHEEEEHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
PYRKALPFEAVTSEVRRCRGTQFDPEIVDIFLSIPRERWEVVGKRHFIVG
CHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLPTILTVDDEEMIRDLMVTALSREGYRCFQAGSAEEARSVLQNNKIDLAILDIMMPGLS
CCCCEEEECHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCCCEEEEEHHCCCCC
GDDFLKELKVLSPETVVLMVTALSDTETAMKCIHLGAYDYILKPFDIDRVLLTIHNALEK
CHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHH
QRLLAENREYQANLEKKVDEQTRQIRMAMEDLNLAYNHTLTALVRALDAREKETGSHSER
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
VMTYTLLLGEAMGVPEKDLSVMARGALLHDIGKVGISDNILLKPAKLDEREWMEMMRHPQ
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCC
LGYEILSGIKFLKGPAEIVFAHHERFDGSGYPKGLSRSGIPLGARIFSLVDTLDAMTSDR
CHHHHHHHHHHHCCCHHEEEEHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
PYRKALPFEAVTSEVRRCRGTQFDPEIVDIFLSIPRERWEVVGKRHFIVG
CHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]