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The map label for this gene is tilS [H]

Identifier: 78223098

GI number: 78223098

Start: 2105996

End: 2107402

Strand: Reverse

Name: tilS [H]

Synonym: Gmet_1891

Alternate gene names: 78223098

Gene position: 2107402-2105996 (Counterclockwise)

Preceding gene: 78223099

Following gene: 78223097

Centisome position: 52.72

GC content: 60.2

Gene sequence:

>1407_bases
GTGAGATCGGTCCACGCAAAAGTCCTTGCCTTTTCCACTGAGCATTCCCTTTTCACTCCCGGTGATACGGTTGTTGTTGC
CGTTTCCGGCGGTGCCGACTCTGTTGCCCTTCTCGATATCCTCATCTCATTGTCAGACCTCCGTTTGAACCTTGTCGTCG
CCCACTTGAACCATTCTCTGCGTGGCGCCGAGTCTGACGGAGACGAGCGCTTTGTCGCCGACCTCGCCGCCTCCCGCGGC
CTTCCTTTCGCAGGTGAAAAAGCTGACGTGCGGGCCTTGAGTATCCGGGAGCGCCTCTCCCTGGAGGATGCTGGTCGCCG
TGCCCGTTATGAATTCTTCGACCGGGTAGCGACGCAGTACGGCGCCCGCTCCGTGGCCCTTGCCCACCATGCCGACGACC
AGGCCGAGACCCTGCTGCTGAGGCTTCTTCGAGGGGCTGGCGTCACGGGGCTTTCTGCCATGGCCCCCCGCAGGGACGGG
CGGTTCGTGCGTCCGCTCCTCACGCTCTCACGGTCCGAGATTGAAGGGTACCTCCATGGCCGGGGGCTTCGCTTCCGTAT
CGACTCATCCAACGCTGACACGGCCTTTCTCCGTAATCGCATCCGTCACGAGCTCATCCCCCATCTCGTTCGTTTTAATC
CTGAGATTTCCCGACGCCTCGCGGTGACTGCCGATCTTCTTGCCGTAGATGAGGAGGTGCTTGAAACCGTTGCTGACGAG
GCTCTGAAGCGGACTGCACGGGAGCACTCCGGTGAAGGCGTGATTCTTGACGTGGCCGGGTTGCTCCGTGAGCCACGGGG
AGTGCGCTACCGTATCTACCGCCGGGCCGTTGCCCTCGCAATGGGAACGTTACGGGGGCTTAGCTACAAACACGTACTGC
AGATAGATGGTCTTGTCCGATCACCAAAACCGAACCTCCATATCCCTGTACCGGGAGGGGTAGGGGTTGTTCGTGCTTAC
GGACTCGTAACATTCAAGGCGGCGGGAGTTGCCGGTATTGAGTGGGAAGATGAACTCATCATCGAGGGGCCGGGATGTTA
TCCGCTGCCCGATGGTGGGGAGATGGTGATTGATGTCGTTTCGCCCCCCGCGGATTTGGTAACGCTCCCTTCGGAGCGGG
CATGCTTTGACCTTGATTGCGCGCCGTTTCCCTGGGTGGTGAGAGGGTTTCGCCCCGGCGACCGGATCAACCCCCTCGGA
ATGACGGGGACCAAGAAGGTCAAGGATATCTTCATCGACGAGAAAGTCCCGCGGGAACGCCGGCGGCTCGTTCCCCTGGT
TTTCAGCAACAGTGAACTTGTCTGGGTCTGCGGGTACCGTACCGGCCACCATGCCCGTGTCACTGAAAAGACTGTATGCA
CCGTGCTCGCAGAGGTCCGAACCAGCACGGGAATTGCCCTTAAATGA

Upstream 100 bases:

>100_bases
CAAGTCCCTGCACCGCCACCATATCTAGACTGACCCCTCTTGGACTAAGAGGGGTTTTTTAGTTTATCCCCTTTGCATGA
GAGCTTCCAGGAGTGAGCCG

Downstream 100 bases:

>100_bases
CTTGTCAACGGCCATGGGTTATGATAGCTTTTTTCAATTAGTTAGATACATCGCACATACAATGGCCGAAAGGCCGTAGG
ACACATCCACGGGGGTTATC

Product: tRNA(Ile)-lysidine synthetase-like

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MRSVHAKVLAFSTEHSLFTPGDTVVVAVSGGADSVALLDILISLSDLRLNLVVAHLNHSLRGAESDGDERFVADLAASRG
LPFAGEKADVRALSIRERLSLEDAGRRARYEFFDRVATQYGARSVALAHHADDQAETLLLRLLRGAGVTGLSAMAPRRDG
RFVRPLLTLSRSEIEGYLHGRGLRFRIDSSNADTAFLRNRIRHELIPHLVRFNPEISRRLAVTADLLAVDEEVLETVADE
ALKRTAREHSGEGVILDVAGLLREPRGVRYRIYRRAVALAMGTLRGLSYKHVLQIDGLVRSPKPNLHIPVPGGVGVVRAY
GLVTFKAAGVAGIEWEDELIIEGPGCYPLPDGGEMVIDVVSPPADLVTLPSERACFDLDCAPFPWVVRGFRPGDRINPLG
MTGTKKVKDIFIDEKVPRERRRLVPLVFSNSELVWVCGYRTGHHARVTEKTVCTVLAEVRTSTGIALK

Sequences:

>Translated_468_residues
MRSVHAKVLAFSTEHSLFTPGDTVVVAVSGGADSVALLDILISLSDLRLNLVVAHLNHSLRGAESDGDERFVADLAASRG
LPFAGEKADVRALSIRERLSLEDAGRRARYEFFDRVATQYGARSVALAHHADDQAETLLLRLLRGAGVTGLSAMAPRRDG
RFVRPLLTLSRSEIEGYLHGRGLRFRIDSSNADTAFLRNRIRHELIPHLVRFNPEISRRLAVTADLLAVDEEVLETVADE
ALKRTAREHSGEGVILDVAGLLREPRGVRYRIYRRAVALAMGTLRGLSYKHVLQIDGLVRSPKPNLHIPVPGGVGVVRAY
GLVTFKAAGVAGIEWEDELIIEGPGCYPLPDGGEMVIDVVSPPADLVTLPSERACFDLDCAPFPWVVRGFRPGDRINPLG
MTGTKKVKDIFIDEKVPRERRRLVPLVFSNSELVWVCGYRTGHHARVTEKTVCTVLAEVRTSTGIALK
>Mature_468_residues
MRSVHAKVLAFSTEHSLFTPGDTVVVAVSGGADSVALLDILISLSDLRLNLVVAHLNHSLRGAESDGDERFVADLAASRG
LPFAGEKADVRALSIRERLSLEDAGRRARYEFFDRVATQYGARSVALAHHADDQAETLLLRLLRGAGVTGLSAMAPRRDG
RFVRPLLTLSRSEIEGYLHGRGLRFRIDSSNADTAFLRNRIRHELIPHLVRFNPEISRRLAVTADLLAVDEEVLETVADE
ALKRTAREHSGEGVILDVAGLLREPRGVRYRIYRRAVALAMGTLRGLSYKHVLQIDGLVRSPKPNLHIPVPGGVGVVRAY
GLVTFKAAGVAGIEWEDELIIEGPGCYPLPDGGEMVIDVVSPPADLVTLPSERACFDLDCAPFPWVVRGFRPGDRINPLG
MTGTKKVKDIFIDEKVPRERRRLVPLVFSNSELVWVCGYRTGHHARVTEKTVCTVLAEVRTSTGIALK

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=427, Percent_Identity=27.8688524590164, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 51337; Mature: 51337

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSVHAKVLAFSTEHSLFTPGDTVVVAVSGGADSVALLDILISLSDLRLNLVVAHLNHSL
CCCCEEEEEEEECCCCEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHEEEEEEECCCCC
RGAESDGDERFVADLAASRGLPFAGEKADVRALSIRERLSLEDAGRRARYEFFDRVATQY
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHCCCHHHHHHHHHHHHHH
GARSVALAHHADDQAETLLLRLLRGAGVTGLSAMAPRRDGRFVRPLLTLSRSEIEGYLHG
CCCEEEEEECCCCHHHHHHHHHHHCCCCCCHHHCCCCCCCCEEHHHHHHHHHHHHHEEEC
RGLRFRIDSSNADTAFLRNRIRHELIPHLVRFNPEISRRLAVTADLLAVDEEVLETVADE
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALKRTAREHSGEGVILDVAGLLREPRGVRYRIYRRAVALAMGTLRGLSYKHVLQIDGLVR
HHHHHHHHCCCCEEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEC
SPKPNLHIPVPGGVGVVRAYGLVTFKAAGVAGIEWEDELIIEGPGCYPLPDGGEMVIDVV
CCCCCEEEECCCCHHHHHHHHEEEEEECCEECCCCCCCEEEECCCCCCCCCCCCEEEEEE
SPPADLVTLPSERACFDLDCAPFPWVVRGFRPGDRINPLGMTGTKKVKDIFIDEKVPRER
CCCCCEEECCCCCCEEECCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHH
RRLVPLVFSNSELVWVCGYRTGHHARVTEKTVCTVLAEVRTSTGIALK
HCEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRSVHAKVLAFSTEHSLFTPGDTVVVAVSGGADSVALLDILISLSDLRLNLVVAHLNHSL
CCCCEEEEEEEECCCCEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHEEEEEEECCCCC
RGAESDGDERFVADLAASRGLPFAGEKADVRALSIRERLSLEDAGRRARYEFFDRVATQY
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHCCCHHHHHHHHHHHHHH
GARSVALAHHADDQAETLLLRLLRGAGVTGLSAMAPRRDGRFVRPLLTLSRSEIEGYLHG
CCCEEEEEECCCCHHHHHHHHHHHCCCCCCHHHCCCCCCCCEEHHHHHHHHHHHHHEEEC
RGLRFRIDSSNADTAFLRNRIRHELIPHLVRFNPEISRRLAVTADLLAVDEEVLETVADE
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALKRTAREHSGEGVILDVAGLLREPRGVRYRIYRRAVALAMGTLRGLSYKHVLQIDGLVR
HHHHHHHHCCCCEEEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEC
SPKPNLHIPVPGGVGVVRAYGLVTFKAAGVAGIEWEDELIIEGPGCYPLPDGGEMVIDVV
CCCCCEEEECCCCHHHHHHHHEEEEEECCEECCCCCCCEEEECCCCCCCCCCCCEEEEEE
SPPADLVTLPSERACFDLDCAPFPWVVRGFRPGDRINPLGMTGTKKVKDIFIDEKVPRER
CCCCCEEECCCCCCEEECCCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHH
RRLVPLVFSNSELVWVCGYRTGHHARVTEKTVCTVLAEVRTSTGIALK
HCEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA