Click here to switch to the map view.

The map label for this gene is rph

Identifier: 78223083

GI number: 78223083

Start: 2091189

End: 2091905

Strand: Reverse

Name: rph

Synonym: Gmet_1876

Alternate gene names: 78223083

Gene position: 2091905-2091189 (Counterclockwise)

Preceding gene: 78223084

Following gene: 78223082

Centisome position: 52.33

GC content: 53.84

Gene sequence:

>717_bases
ATGCGTTCGGACGGCCGTGAGGCGGGGAGCCTCAGGGGCATAAATATTACTCGGCATTATATCAAGCATGCGGAAGGGGC
TGTGCTTGTGGAGTTTGGCGACACCAAGGTCATCTGTACCGCATCGGTGGAAGAGAGTGTCCCTCCATTTCTCCGGGGGA
AGGGGACCGGTTGGGTGACTGCCGAGTATTCGATGCTTCCCCGTTCGACTCACACGCGTTCTTCGCGCGAAGCTGCCCGT
GGAAAAATCGGTGGAAGGACCCACGAAATACAGAGGTTGATCGGCCGTTCGCTTCGTGCCGTCACAGATTTGACGCTTCT
CGGGGAGAGAAGTGTCCTCATAGACTGTGATGTTATTCAGGCTGACGGAGGAACCAGGACTGCTTCCATAACCGGAGCCT
ATGTCGCTTTGGTGGATGCCTTGCACTTGCTTGTGGAGCGTGGTGTGTTGCCGTTGCTCCCCATCAAGGAAGCCGTTGCG
GCGGTCAGTGTCGGAATAGTGGATGGCAACATTCTATTGGATCTGAATTACGTGGAAGATTCAGCGGCCGAAGTCGACAT
GAATTTTGTCATGACATCATCTGACCGTTTTGTAGAAGTACAGGGTACTGCCGAGTCGGAACCCTTTACCGTTGAACAGA
TGGACGGCATGCGTGTAGCTGCAGTGGAGGGAATACGAAAGCTTTTCTCGATTCAGAATGAGGCTCTTTCCCGATGA

Upstream 100 bases:

>100_bases
ACTACGTGAGGTGTAAGGTCTCGTCATGTCATGCAGATTGACATCCTCCAGCCTATCGTTTAGTGTACGCAGCTTAAAAT
TTAGCACAGAGAGGTGAAAT

Downstream 100 bases:

>100_bases
CTGACTTGATTGTCGCCACGAGGAATAAGGGGAAGCTCCTGGAAATTGGAAAGATCCTAGAAGGAGTTCACTGCAGAATA
TACTCTCTTGCTGATTTTCC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRSDGREAGSLRGINITRHYIKHAEGAVLVEFGDTKVICTASVEESVPPFLRGKGTGWVTAEYSMLPRSTHTRSSREAAR
GKIGGRTHEIQRLIGRSLRAVTDLTLLGERSVLIDCDVIQADGGTRTASITGAYVALVDALHLLVERGVLPLLPIKEAVA
AVSVGIVDGNILLDLNYVEDSAAEVDMNFVMTSSDRFVEVQGTAESEPFTVEQMDGMRVAAVEGIRKLFSIQNEALSR

Sequences:

>Translated_238_residues
MRSDGREAGSLRGINITRHYIKHAEGAVLVEFGDTKVICTASVEESVPPFLRGKGTGWVTAEYSMLPRSTHTRSSREAAR
GKIGGRTHEIQRLIGRSLRAVTDLTLLGERSVLIDCDVIQADGGTRTASITGAYVALVDALHLLVERGVLPLLPIKEAVA
AVSVGIVDGNILLDLNYVEDSAAEVDMNFVMTSSDRFVEVQGTAESEPFTVEQMDGMRVAAVEGIRKLFSIQNEALSR
>Mature_238_residues
MRSDGREAGSLRGINITRHYIKHAEGAVLVEFGDTKVICTASVEESVPPFLRGKGTGWVTAEYSMLPRSTHTRSSREAAR
GKIGGRTHEIQRLIGRSLRAVTDLTLLGERSVLIDCDVIQADGGTRTASITGAYVALVDALHLLVERGVLPLLPIKEAVA
AVSVGIVDGNILLDLNYVEDSAAEVDMNFVMTSSDRFVEVQGTAESEPFTVEQMDGMRVAAVEGIRKLFSIQNEALSR

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Homo sapiens, GI9506689, Length=188, Percent_Identity=32.4468085106383, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI157672248, Length=210, Percent_Identity=69.0476190476191, Blast_Score=298, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI71981632, Length=184, Percent_Identity=29.8913043478261, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_GEOMG (Q39UG9)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384830.1
- HSSP:   O29756
- ProteinModelPortal:   Q39UG9
- SMR:   Q39UG9
- STRING:   Q39UG9
- GeneID:   3740278
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1876
- NMPDR:   fig|269799.3.peg.2139
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- PhylomeDB:   Q39UG9
- ProtClustDB:   PRK00173
- BioCyc:   GMET269799:GMET_1876-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25725; Mature: 25725

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSDGREAGSLRGINITRHYIKHAEGAVLVEFGDTKVICTASVEESVPPFLRGKGTGWVT
CCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCCCHHCCCCCEEEE
AEYSMLPRSTHTRSSREAARGKIGGRTHEIQRLIGRSLRAVTDLTLLGERSVLIDCDVIQ
EEEEECCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEECCCEEEEEEEEEE
ADGGTRTASITGAYVALVDALHLLVERGVLPLLPIKEAVAAVSVGIVDGNILLDLNYVED
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEEEEECCEEEEEEEECCC
SAAEVDMNFVMTSSDRFVEVQGTAESEPFTVEQMDGMRVAAVEGIRKLFSIQNEALSR
CCCEEEEEEEEECCCCEEEEECCCCCCCEEEECCCCEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRSDGREAGSLRGINITRHYIKHAEGAVLVEFGDTKVICTASVEESVPPFLRGKGTGWVT
CCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCCCHHCCCCCEEEE
AEYSMLPRSTHTRSSREAARGKIGGRTHEIQRLIGRSLRAVTDLTLLGERSVLIDCDVIQ
EEEEECCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEECCCEEEEEEEEEE
ADGGTRTASITGAYVALVDALHLLVERGVLPLLPIKEAVAAVSVGIVDGNILLDLNYVED
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEEEEECCEEEEEEEECCC
SAAEVDMNFVMTSSDRFVEVQGTAESEPFTVEQMDGMRVAAVEGIRKLFSIQNEALSR
CCCEEEEEEEEECCCCEEEEECCCCCCCEEEECCCCEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA