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The map label for this gene is braC [H]

Identifier: 78223030

GI number: 78223030

Start: 2036801

End: 2037943

Strand: Reverse

Name: braC [H]

Synonym: Gmet_1823

Alternate gene names: 78223030

Gene position: 2037943-2036801 (Counterclockwise)

Preceding gene: 78223031

Following gene: 78223029

Centisome position: 50.98

GC content: 57.66

Gene sequence:

>1143_bases
ATGCTGAAGAAAAGTTCTTTCATACTGACGTTTGTCGTGTCGCTGGCTCTTCTGGCCGGAAGCGCCCTTGCGGCTCCCCC
CATCAAGATCGGCGGGCTCTTCGCTGTGACTGGTCCTGCATCGTTCCTTGGCGAGCCTGAGCGCAACACCGCCCAGATGG
TGGTGAACGGGATCAATGCTGCCGGTGGCGTCAAAGGTCGCAAGCTTGAACTGATCGCCTACGACACGCAAGGGGATGCC
ACCAAGGCGGTGCAGGCTGCCAACCGCCTCATCAAAGAGGACAAGGTTGCCGCAATCATAGGCCCCAGCACCACTGGTGA
CACCATGGCCGTCATTCCGGTTGTGGAACGGGCAAGAATTCCGATGATTTCCTGTGCCGCCGGAAGCAAAATCACCGAGC
CGGTCAAAAAGTGGATTTTCAAGACCGCTCAGAATGATGGCCTCGCCGTGGCGAGAATCTATGGGGATCTGAAGAGGAAA
AAAATATCAAAGATTGCTATCCTCACCGTTTCCGACGGTTTCGGTTCGTCGGGACGCGAGCAGCTGAAGGCGCAGGCATC
GGCCTACGGGATCAGGATCGTGTCTGACGACACCTATGGCCCGAAGGATACCGACATGACAGCCCAGCTTACCAAAATCA
AGGGGAGCGGCGCCCAGGCCGTCATCTGCTGGGGCACCAATCCGGGGCCGGCAGTGGTTGCCCGCAACGCCAAGCAGCTT
GGCCTGAAGCTTCCCCTCTACATGAGCCACGGCGTTTCCTCCAAGAAGTTCATCGGTCTTGCCGGAGGTGCTGCCGAAGG
AATCCTTCTCCCGTCCGGACGAGTTATCGTGGCCGATCTGCTTCCCAATTCAGACCGTCAGAAAAAGTCCCTTCTTGCCT
TCGTGAAGGATTACCAGAAGCACTACAAGGCGGAGGGTGACCACTTTGGCGGCCATGCCTGGGATGCGATCATGCTTCTC
AAAGGCGCTATCGAGCGTGGCGGCGAAAATCCGGCAGCCATACGGGACCAGCTGGAACGGACGCGGGGCTTTGCCGGCAT
CGGCGGTGTTTTCAGCTACTCTCCTTCCGATCACGCGGGACTCACCAAGGACGCTTTCGTCCTTGTGGAGATAAGAAAAC
AAGATTTCGCCCTGGTCAAGTAA

Upstream 100 bases:

>100_bases
GGCGCCCGGCTCTACGCCATGTGATCGCTACGCTGGGAGAGCCGCGGGGATCATCAGGGGAGACTGGGAGTTGGTGAAGT
AATCAACGGGGAGGTTGTCC

Downstream 100 bases:

>100_bases
CCGCTTAATCATACATGGACCTTCAACCAATCAAGGGAGCGCAGGCTTTGTCGGTCTGCGCTCCCTCCTTGTGAGCCAGA
CACATAATCTATGCATATCG

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MLKKSSFILTFVVSLALLAGSALAAPPIKIGGLFAVTGPASFLGEPERNTAQMVVNGINAAGGVKGRKLELIAYDTQGDA
TKAVQAANRLIKEDKVAAIIGPSTTGDTMAVIPVVERARIPMISCAAGSKITEPVKKWIFKTAQNDGLAVARIYGDLKRK
KISKIAILTVSDGFGSSGREQLKAQASAYGIRIVSDDTYGPKDTDMTAQLTKIKGSGAQAVICWGTNPGPAVVARNAKQL
GLKLPLYMSHGVSSKKFIGLAGGAAEGILLPSGRVIVADLLPNSDRQKKSLLAFVKDYQKHYKAEGDHFGGHAWDAIMLL
KGAIERGGENPAAIRDQLERTRGFAGIGGVFSYSPSDHAGLTKDAFVLVEIRKQDFALVK

Sequences:

>Translated_380_residues
MLKKSSFILTFVVSLALLAGSALAAPPIKIGGLFAVTGPASFLGEPERNTAQMVVNGINAAGGVKGRKLELIAYDTQGDA
TKAVQAANRLIKEDKVAAIIGPSTTGDTMAVIPVVERARIPMISCAAGSKITEPVKKWIFKTAQNDGLAVARIYGDLKRK
KISKIAILTVSDGFGSSGREQLKAQASAYGIRIVSDDTYGPKDTDMTAQLTKIKGSGAQAVICWGTNPGPAVVARNAKQL
GLKLPLYMSHGVSSKKFIGLAGGAAEGILLPSGRVIVADLLPNSDRQKKSLLAFVKDYQKHYKAEGDHFGGHAWDAIMLL
KGAIERGGENPAAIRDQLERTRGFAGIGGVFSYSPSDHAGLTKDAFVLVEIRKQDFALVK
>Mature_380_residues
MLKKSSFILTFVVSLALLAGSALAAPPIKIGGLFAVTGPASFLGEPERNTAQMVVNGINAAGGVKGRKLELIAYDTQGDA
TKAVQAANRLIKEDKVAAIIGPSTTGDTMAVIPVVERARIPMISCAAGSKITEPVKKWIFKTAQNDGLAVARIYGDLKRK
KISKIAILTVSDGFGSSGREQLKAQASAYGIRIVSDDTYGPKDTDMTAQLTKIKGSGAQAVICWGTNPGPAVVARNAKQL
GLKLPLYMSHGVSSKKFIGLAGGAAEGILLPSGRVIVADLLPNSDRQKKSLLAFVKDYQKHYKAEGDHFGGHAWDAIMLL
KGAIERGGENPAAIRDQLERTRGFAGIGGVFSYSPSDHAGLTKDAFVLVEIRKQDFALVK

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=274, Percent_Identity=27.7372262773723, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI48994941, Length=266, Percent_Identity=28.1954887218045, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40100; Mature: 40100

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKSSFILTFVVSLALLAGSALAAPPIKIGGLFAVTGPASFLGEPERNTAQMVVNGINA
CCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCEEEECCCHHHCCCCCCCHHHHHEECCCC
AGGVKGRKLELIAYDTQGDATKAVQAANRLIKEDKVAAIIGPSTTGDTMAVIPVVERARI
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEHHCCCC
PMISCAAGSKITEPVKKWIFKTAQNDGLAVARIYGDLKRKKISKIAILTVSDGFGSSGRE
CEEEECCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCEEEEEEEECCCCCCCHH
QLKAQASAYGIRIVSDDTYGPKDTDMTAQLTKIKGSGAQAVICWGTNPGPAVVARNAKQL
HHHHHHHHEEEEEEECCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCCEEEECCHHHC
GLKLPLYMSHGVSSKKFIGLAGGAAEGILLPSGRVIVADLLPNSDRQKKSLLAFVKDYQK
CCCCCCHHHCCCCCCEEEEEECCCCCCEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHH
HYKAEGDHFGGHAWDAIMLLKGAIERGGENPAAIRDQLERTRGFAGIGGVFSYSPSDHAG
HHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCC
LTKDAFVLVEIRKQDFALVK
CCCCEEEEEEEECCCCEECC
>Mature Secondary Structure
MLKKSSFILTFVVSLALLAGSALAAPPIKIGGLFAVTGPASFLGEPERNTAQMVVNGINA
CCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCEEEECCCHHHCCCCCCCHHHHHEECCCC
AGGVKGRKLELIAYDTQGDATKAVQAANRLIKEDKVAAIIGPSTTGDTMAVIPVVERARI
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEHHCCCC
PMISCAAGSKITEPVKKWIFKTAQNDGLAVARIYGDLKRKKISKIAILTVSDGFGSSGRE
CEEEECCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCEEEEEEEECCCCCCCHH
QLKAQASAYGIRIVSDDTYGPKDTDMTAQLTKIKGSGAQAVICWGTNPGPAVVARNAKQL
HHHHHHHHEEEEEEECCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCCEEEECCHHHC
GLKLPLYMSHGVSSKKFIGLAGGAAEGILLPSGRVIVADLLPNSDRQKKSLLAFVKDYQK
CCCCCCHHHCCCCCCEEEEEECCCCCCEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHH
HYKAEGDHFGGHAWDAIMLLKGAIERGGENPAAIRDQLERTRGFAGIGGVFSYSPSDHAG
HHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCC
LTKDAFVLVEIRKQDFALVK
CCCCEEEEEEEECCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]