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The map label for this gene is hmeA [H]

Identifier: 78223018

GI number: 78223018

Start: 2027126

End: 2027839

Strand: Direct

Name: hmeA [H]

Synonym: Gmet_1811

Alternate gene names: 78223018

Gene position: 2027126-2027839 (Clockwise)

Preceding gene: 78223017

Following gene: 78223019

Centisome position: 50.71

GC content: 59.1

Gene sequence:

>714_bases
ATGGAAACAATGACGAGTCAGTACAGATGGGGGATGGTTGTCGACCTTGATCGGTGCACCGGCTGCGGCGCTTGCGTTGT
TGCCTGTCAGGCGGAGAACAACGTGGCCATCTGCGGCAAGAAGGAGATATCCGAAGGACGCAACATGCACTGGTTACGCA
TCGAGCGGTTCTGGGTGGGACAGTATCCGGACGTGCGGGCGCGCTTTCTCCCGGTACTCTGTCAGCACTGTCATGACGCC
CCCTGTGAGCCGGTCTGCCCGGTTTATGCCAGCTACCACAACCCCGACGGTCTTAATGGGCAGGTGTACAACCGCTGCGT
CGGCACCAGGTACTGCGGTAATAACTGCCCCTATGCCGTAAGAGTCTTCAACTTCTACAACCACGAATGGCCCAACCCCC
TCGATAATCAACTTTCTCCGGACATGACCGTGCGTACCCGGGGAGTGATGGAAAAATGCACTTTCTGCATCCAGCGCATC
CGAAGGGCCAAGGAAGATGCCAAGTCCGAAAATCGCACCATTCGTGACGGAGAGATACAACCGGCCTGCGCCCAGACCTG
TCCAACCGACGCTCTGGTGTTCGGGAACATGGCCGACCCTCGGAGCCGGGTCGCCATCCTTTCCCGCAGCCCCAGGCGCT
TCCGGCTGCTGGAGCATCTGGGCACCCACCCCTCGATTTGCTATCTCAAGGGAGGAGGACGCGAGCATGTCTGA

Upstream 100 bases:

>100_bases
GGGGAACTGGTTACGGCAGGACATCCCCAGGGAAGCCTCCGGAGCGAGTTGGTCGAGATATAGGGAAGCCGCGCAAGCGG
TCATCGGGATTGAGTCGATT

Downstream 100 bases:

>100_bases
GCCGACACTTCCTGCCGCCAAGAACTCCAGCCAGTTGAATGACGAGATGTTGTCCTCCCTGCGGACCCCCACCTGGAGCT
GGTACGCGCTCGCGCTATTC

Product: 4Fe-4S ferredoxin, iron-sulfur binding protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Hme subunit A [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
METMTSQYRWGMVVDLDRCTGCGACVVACQAENNVAICGKKEISEGRNMHWLRIERFWVGQYPDVRARFLPVLCQHCHDA
PCEPVCPVYASYHNPDGLNGQVYNRCVGTRYCGNNCPYAVRVFNFYNHEWPNPLDNQLSPDMTVRTRGVMEKCTFCIQRI
RRAKEDAKSENRTIRDGEIQPACAQTCPTDALVFGNMADPRSRVAILSRSPRRFRLLEHLGTHPSICYLKGGGREHV

Sequences:

>Translated_237_residues
METMTSQYRWGMVVDLDRCTGCGACVVACQAENNVAICGKKEISEGRNMHWLRIERFWVGQYPDVRARFLPVLCQHCHDA
PCEPVCPVYASYHNPDGLNGQVYNRCVGTRYCGNNCPYAVRVFNFYNHEWPNPLDNQLSPDMTVRTRGVMEKCTFCIQRI
RRAKEDAKSENRTIRDGEIQPACAQTCPTDALVFGNMADPRSRVAILSRSPRRFRLLEHLGTHPSICYLKGGGREHV
>Mature_237_residues
METMTSQYRWGMVVDLDRCTGCGACVVACQAENNVAICGKKEISEGRNMHWLRIERFWVGQYPDVRARFLPVLCQHCHDA
PCEPVCPVYASYHNPDGLNGQVYNRCVGTRYCGNNCPYAVRVFNFYNHEWPNPLDNQLSPDMTVRTRGVMEKCTFCIQRI
RRAKEDAKSENRTIRDGEIQPACAQTCPTDALVFGNMADPRSRVAILSRSPRRFRLLEHLGTHPSICYLKGGGREHV

Specific function: Has menaquinol-oxidizing activity. HmeA, hmeB and hmeE subunits may together catalyze electron transfer from menaquinol to cytochrome c [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=219, Percent_Identity=33.3333333333333, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1787122, Length=206, Percent_Identity=28.6407766990291, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1787872, Length=206, Percent_Identity=28.6407766990291, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI226510944, Length=211, Percent_Identity=27.9620853080569, Blast_Score=78, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 27005; Mature: 27005

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
10.1 %Cys+Met (Translated Protein)
7.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
10.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METMTSQYRWGMVVDLDRCTGCGACVVACQAENNVAICGKKEISEGRNMHWLRIERFWVG
CCCCCCCCCCCEEEEHHCCCCCCEEEEEEECCCCEEEECCHHHHCCCCEEEEEEEEEECC
QYPDVRARFLPVLCQHCHDAPCEPVCPVYASYHNPDGLNGQVYNRCVGTRYCGNNCPYAV
CCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCEEE
RVFNFYNHEWPNPLDNQLSPDMTVRTRGVMEKCTFCIQRIRRAKEDAKSENRTIRDGEIQ
EEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PACAQTCPTDALVFGNMADPRSRVAILSRSPRRFRLLEHLGTHPSICYLKGGGREHV
CHHHHHCCCCEEEECCCCCHHHHEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCC
>Mature Secondary Structure
METMTSQYRWGMVVDLDRCTGCGACVVACQAENNVAICGKKEISEGRNMHWLRIERFWVG
CCCCCCCCCCCEEEEHHCCCCCCEEEEEEECCCCEEEECCHHHHCCCCEEEEEEEEEECC
QYPDVRARFLPVLCQHCHDAPCEPVCPVYASYHNPDGLNGQVYNRCVGTRYCGNNCPYAV
CCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCEEE
RVFNFYNHEWPNPLDNQLSPDMTVRTRGVMEKCTFCIQRIRRAKEDAKSENRTIRDGEIQ
EEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PACAQTCPTDALVFGNMADPRSRVAILSRSPRRFRLLEHLGTHPSICYLKGGGREHV
CHHHHHCCCCEEEECCCCCHHHHEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475; 11952791 [H]