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The map label for this gene is phsA [H]

Identifier: 78223017

GI number: 78223017

Start: 2024896

End: 2027088

Strand: Direct

Name: phsA [H]

Synonym: Gmet_1810

Alternate gene names: 78223017

Gene position: 2024896-2027088 (Clockwise)

Preceding gene: 78223016

Following gene: 78223018

Centisome position: 50.66

GC content: 61.38

Gene sequence:

>2193_bases
ATGAAACGGAGAACGTTTTTACAGCTTGGTACAGTAATAGCTGCCGGCGCCGTACTGGATGGATGCCGGTCAAAAAATGA
GCAGCTCATCCCCTATCTCATTCCGCCGGATGAGGGGATAACTCCCGGCAAAGGTACTTATTATGCCAGCTCCTGCAATG
CATGTCCTGCCGGCTGCGGCATCCTAGTCCGAGTGTCCGAGGGACGGGCAAAGAAGATTGAGGGAAACCCGGAACATCCC
ATCAATAGAGGGAAACTCTGCGCCCGGGGGCAGGCCGTTGTTCAGGAACTTTACCATCCCGACCGCGTGCCTCATCCCCT
CAAACGGAATGGCCCCCGTGGTTCCGGTGCCTTCACGCGGATTTCCTGGCAAGAGGGTATTGGGCTGCTCACGGATAGGC
TCAAAGCTTTGCAGCGGGAACAGAGCACTAATGGCCTGGCTCTGCTGACGCCTCATTTGCGGGGTACCCTGGCGGAACTC
ACGGGTCGCTTCATGCGCACGTTCGGCTCGACCAACCACGTCTCCTATGAGTTGCTCACCCCCGACTTGCTTCGGATTGC
TGCCCGTCGTAGCTTCGGGCAGCCTACCCTTCCCTATTACGACATCGCCGAAACGCGCTATCTGCTCAGTTTCGGGGCTG
ATTTTGTGGACTCTCACCTGTCGCCGGTCAAGTATGGTTCCGCCTTTGGTGAGATGCGCCAGGGCCGTGACACGGTTCGC
GGGCATTTCACCTATGTGGGGGGGCGCATGTCACTGACTGCCGCCTCGGCCGACCGCTGGATGCCTGCCAAGCCGGGGAG
CGAAGGAACCCTGGCCCTTGGCATTGCACGCCTGATTCTGGCCGAGTCGCTTTACGACAGCGGAGCACTCGCGATGAACG
GGCTTGAGGCAAAGAAACTGCTGGCCGCGCTGGACAGATACGATCTCCCCCGAGTGGCGCAGCTCTCCGGTCTGCCGCAA
GGAGCGATCGCTGAGGTGGCACGGGAGTTTGCCTCGACCCGTCCCGCACTCGCTCTGGCCGGGGAGATGGTTGCCTTTCA
GAGCAACGGCCCTGAATCGGTCCGTGCGGTCCACCTGCTCAATCTCCTGGTGGGAAGCCTGAATCGGCCGGGTGGGATCT
ACCCCGATGCAGGGTCCCCATCCGGACCGGAAAATTCCTTTGCCGAACTCATCGCTCTTATCGAGACGATGCGCGGCGGC
CACGTGAAGGTGGCCATGATTCATGGCGATCCGCTCTATTCCGTTCCTCCGGCGACTGGGTTTCACGACGCACTGGCAAA
GGTTCCGTTCATCGTCAGCTTTTCCTCGCTGCTCGACGATACCGCCCTGCACGCGGACTTGGTCCTTCCGGACCACGCCG
CCCTGGAAAGCTGGGGAGACGTGATACCGGTCGCCGGCACCCGGGAACCGCTGGTGGGGCTTATGCAGCCGGTTGTGGAG
CCTTTGTTCGATACCCGCCAGTTCCCCGAAGTGTTGCTGGCTACTGCCCATGAGCTGGGAGGCCGGATGGCTGCAGCATT
ACCGGACGAGTCGTACCTGGAGTTGCTGAAGGGAGCGGTCAGGAAGCAAGCCGGATTTGGGGCCGGCGTGGATTTCGAAG
CGGCGTGGGTCGACCTGCTTCAGCACGGAGGGCTCTTTAAAACCAAGAGTGTCCAGCCCACCGGTTATCGATGGTCCTCG
GACGCACCACCGCCATTGCCCGAGGACCCGGACTTTGCCGGGGACCAGAAGACGTACCCATTCCATCTGATTGCCTACCC
TGCCACCGCCTTTTATGACGGCCGCGGTGCACCGTATCCCTGGCTGCAGCAACTCCCGGACCCGATGACCACCGTCGTTT
GGGGAAGTTGGGTGGAGATCAACCCCGAGACCGCAGCCGAGCACGGGATCGGCTTTGGCGATCTGGTGGAGGTCTCATCG
CCCCAGGGCACACTCCGTCTGCCGGCCGTCGTTTATCCCGGAATCCGTCCGGACATGGTGGCGATCCCCTTGGGGCAGGG
GCATCGGGGGATGGGACGCTATGCCCAGGGGCAAGGGGTAAATCCTCTCGCGCTGGTGGCACGGCAAGGGACGAAACAGC
AGCCTGCCTGGAATGCTACCCGGGTGCGGATAACCAGAATCTCAGGGAATGGGGAACTGGTTACGGCAGGACATCCCCAG
GGAAGCCTCCGGAGCGAGTTGGTCGAGATATAG

Upstream 100 bases:

>100_bases
ACCCGGACCGCAAGCCTCAAGATGGGATGGTGCCTGAACTGCCACCATCAGCATAAGGCCAGTATCGACTGCTGGACGTG
TCACAAGTAGCGGAGCGCAA

Downstream 100 bases:

>100_bases
GGAAGCCGCGCAAGCGGTCATCGGGATTGAGTCGATTATGGAAACAATGACGAGTCAGTACAGATGGGGGATGGTTGTCG
ACCTTGATCGGTGCACCGGC

Product: molybdopterin oxidoreductase Fe4S4 region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 730; Mature: 730

Protein sequence:

>730_residues
MKRRTFLQLGTVIAAGAVLDGCRSKNEQLIPYLIPPDEGITPGKGTYYASSCNACPAGCGILVRVSEGRAKKIEGNPEHP
INRGKLCARGQAVVQELYHPDRVPHPLKRNGPRGSGAFTRISWQEGIGLLTDRLKALQREQSTNGLALLTPHLRGTLAEL
TGRFMRTFGSTNHVSYELLTPDLLRIAARRSFGQPTLPYYDIAETRYLLSFGADFVDSHLSPVKYGSAFGEMRQGRDTVR
GHFTYVGGRMSLTAASADRWMPAKPGSEGTLALGIARLILAESLYDSGALAMNGLEAKKLLAALDRYDLPRVAQLSGLPQ
GAIAEVAREFASTRPALALAGEMVAFQSNGPESVRAVHLLNLLVGSLNRPGGIYPDAGSPSGPENSFAELIALIETMRGG
HVKVAMIHGDPLYSVPPATGFHDALAKVPFIVSFSSLLDDTALHADLVLPDHAALESWGDVIPVAGTREPLVGLMQPVVE
PLFDTRQFPEVLLATAHELGGRMAAALPDESYLELLKGAVRKQAGFGAGVDFEAAWVDLLQHGGLFKTKSVQPTGYRWSS
DAPPPLPEDPDFAGDQKTYPFHLIAYPATAFYDGRGAPYPWLQQLPDPMTTVVWGSWVEINPETAAEHGIGFGDLVEVSS
PQGTLRLPAVVYPGIRPDMVAIPLGQGHRGMGRYAQGQGVNPLALVARQGTKQQPAWNATRVRITRISGNGELVTAGHPQ
GSLRSELVEI

Sequences:

>Translated_730_residues
MKRRTFLQLGTVIAAGAVLDGCRSKNEQLIPYLIPPDEGITPGKGTYYASSCNACPAGCGILVRVSEGRAKKIEGNPEHP
INRGKLCARGQAVVQELYHPDRVPHPLKRNGPRGSGAFTRISWQEGIGLLTDRLKALQREQSTNGLALLTPHLRGTLAEL
TGRFMRTFGSTNHVSYELLTPDLLRIAARRSFGQPTLPYYDIAETRYLLSFGADFVDSHLSPVKYGSAFGEMRQGRDTVR
GHFTYVGGRMSLTAASADRWMPAKPGSEGTLALGIARLILAESLYDSGALAMNGLEAKKLLAALDRYDLPRVAQLSGLPQ
GAIAEVAREFASTRPALALAGEMVAFQSNGPESVRAVHLLNLLVGSLNRPGGIYPDAGSPSGPENSFAELIALIETMRGG
HVKVAMIHGDPLYSVPPATGFHDALAKVPFIVSFSSLLDDTALHADLVLPDHAALESWGDVIPVAGTREPLVGLMQPVVE
PLFDTRQFPEVLLATAHELGGRMAAALPDESYLELLKGAVRKQAGFGAGVDFEAAWVDLLQHGGLFKTKSVQPTGYRWSS
DAPPPLPEDPDFAGDQKTYPFHLIAYPATAFYDGRGAPYPWLQQLPDPMTTVVWGSWVEINPETAAEHGIGFGDLVEVSS
PQGTLRLPAVVYPGIRPDMVAIPLGQGHRGMGRYAQGQGVNPLALVARQGTKQQPAWNATRVRITRISGNGELVTAGHPQ
GSLRSELVEI
>Mature_730_residues
MKRRTFLQLGTVIAAGAVLDGCRSKNEQLIPYLIPPDEGITPGKGTYYASSCNACPAGCGILVRVSEGRAKKIEGNPEHP
INRGKLCARGQAVVQELYHPDRVPHPLKRNGPRGSGAFTRISWQEGIGLLTDRLKALQREQSTNGLALLTPHLRGTLAEL
TGRFMRTFGSTNHVSYELLTPDLLRIAARRSFGQPTLPYYDIAETRYLLSFGADFVDSHLSPVKYGSAFGEMRQGRDTVR
GHFTYVGGRMSLTAASADRWMPAKPGSEGTLALGIARLILAESLYDSGALAMNGLEAKKLLAALDRYDLPRVAQLSGLPQ
GAIAEVAREFASTRPALALAGEMVAFQSNGPESVRAVHLLNLLVGSLNRPGGIYPDAGSPSGPENSFAELIALIETMRGG
HVKVAMIHGDPLYSVPPATGFHDALAKVPFIVSFSSLLDDTALHADLVLPDHAALESWGDVIPVAGTREPLVGLMQPVVE
PLFDTRQFPEVLLATAHELGGRMAAALPDESYLELLKGAVRKQAGFGAGVDFEAAWVDLLQHGGLFKTKSVQPTGYRWSS
DAPPPLPEDPDFAGDQKTYPFHLIAYPATAFYDGRGAPYPWLQQLPDPMTTVVWGSWVEINPETAAEHGIGFGDLVEVSS
PQGTLRLPAVVYPGIRPDMVAIPLGQGHRGMGRYAQGQGVNPLALVARQGTKQQPAWNATRVRITRISGNGELVTAGHPQ
GSLRSELVEI

Specific function: Oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787870, Length=743, Percent_Identity=23.4185733512786, Blast_Score=137, Evalue=3e-33,
Organism=Escherichia coli, GI171474008, Length=735, Percent_Identity=23.6734693877551, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI87081797, Length=734, Percent_Identity=23.9782016348774, Blast_Score=123, Evalue=5e-29,
Organism=Escherichia coli, GI1788534, Length=396, Percent_Identity=21.7171717171717, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI3868719, Length=256, Percent_Identity=25.390625, Blast_Score=77, Evalue=5e-15,
Organism=Escherichia coli, GI3868720, Length=406, Percent_Identity=23.3990147783251, Blast_Score=76, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 78470; Mature: 78470

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRTFLQLGTVIAAGAVLDGCRSKNEQLIPYLIPPDEGITPGKGTYYASSCNACPAGCG
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCEEECCCCCCCCCCE
ILVRVSEGRAKKIEGNPEHPINRGKLCARGQAVVQELYHPDRVPHPLKRNGPRGSGAFTR
EEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCEEE
ISWQEGIGLLTDRLKALQREQSTNGLALLTPHLRGTLAELTGRFMRTFGSTNHVSYELLT
EEHHHCHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
PDLLRIAARRSFGQPTLPYYDIAETRYLLSFGADFVDSHLSPVKYGSAFGEMRQGRDTVR
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHCCCHHHC
GHFTYVGGRMSLTAASADRWMPAKPGSEGTLALGIARLILAESLYDSGALAMNGLEAKKL
EEEEEECCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCHHHHHH
LAALDRYDLPRVAQLSGLPQGAIAEVAREFASTRPALALAGEMVAFQSNGPESVRAVHLL
HHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHCCCHHHHHCCEEEEECCCCHHHHHHHHH
NLLVGSLNRPGGIYPDAGSPSGPENSFAELIALIETMRGGHVKVAMIHGDPLYSVPPATG
HHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCC
FHDALAKVPFIVSFSSLLDDTALHADLVLPDHAALESWGDVIPVAGTREPLVGLMQPVVE
HHHHHHHCCHHEEHHHHHHHHHHHEEEECCCHHHHHHCCCEEECCCCHHHHHHHHHHHHH
PLFDTRQFPEVLLATAHELGGRMAAALPDESYLELLKGAVRKQAGFGAGVDFEAAWVDLL
HHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
QHGGLFKTKSVQPTGYRWSSDAPPPLPEDPDFAGDQKTYPFHLIAYPATAFYDGRGAPYP
HCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEECCCCCCCH
WLQQLPDPMTTVVWGSWVEINPETAAEHGIGFGDLVEVSSPQGTLRLPAVVYPGIRPDMV
HHHHCCCCHHHHEECCEEEECCHHHHHHCCCCCCEEEECCCCCCEECCEEECCCCCCCEE
AIPLGQGHRGMGRYAQGQGVNPLALVARQGTKQQPAWNATRVRITRISGNGELVTAGHPQ
EEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCEEECCCCC
GSLRSELVEI
CHHHHHHHCC
>Mature Secondary Structure
MKRRTFLQLGTVIAAGAVLDGCRSKNEQLIPYLIPPDEGITPGKGTYYASSCNACPAGCG
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCEEECCCCCCCCCCE
ILVRVSEGRAKKIEGNPEHPINRGKLCARGQAVVQELYHPDRVPHPLKRNGPRGSGAFTR
EEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCEEE
ISWQEGIGLLTDRLKALQREQSTNGLALLTPHLRGTLAELTGRFMRTFGSTNHVSYELLT
EEHHHCHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
PDLLRIAARRSFGQPTLPYYDIAETRYLLSFGADFVDSHLSPVKYGSAFGEMRQGRDTVR
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHCCCHHHC
GHFTYVGGRMSLTAASADRWMPAKPGSEGTLALGIARLILAESLYDSGALAMNGLEAKKL
EEEEEECCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCHHHHHH
LAALDRYDLPRVAQLSGLPQGAIAEVAREFASTRPALALAGEMVAFQSNGPESVRAVHLL
HHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHCCCHHHHHCCEEEEECCCCHHHHHHHHH
NLLVGSLNRPGGIYPDAGSPSGPENSFAELIALIETMRGGHVKVAMIHGDPLYSVPPATG
HHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCC
FHDALAKVPFIVSFSSLLDDTALHADLVLPDHAALESWGDVIPVAGTREPLVGLMQPVVE
HHHHHHHCCHHEEHHHHHHHHHHHEEEECCCHHHHHHCCCEEECCCCHHHHHHHHHHHHH
PLFDTRQFPEVLLATAHELGGRMAAALPDESYLELLKGAVRKQAGFGAGVDFEAAWVDLL
HHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
QHGGLFKTKSVQPTGYRWSSDAPPPLPEDPDFAGDQKTYPFHLIAYPATAFYDGRGAPYP
HCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEECCCCCCCH
WLQQLPDPMTTVVWGSWVEINPETAAEHGIGFGDLVEVSSPQGTLRLPAVVYPGIRPDMV
HHHHCCCCHHHHEECCEEEECCHHHHHHCCCCCCEEEECCCCCCEECCEEECCCCCCCEE
AIPLGQGHRGMGRYAQGQGVNPLALVARQGTKQQPAWNATRVRITRISGNGELVTAGHPQ
EEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCEEECCCCC
GSLRSELVEI
CHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7751291; 11677609; 7737516 [H]