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The map label for this gene is amtB [C]

Identifier: 78222947

GI number: 78222947

Start: 1953908

End: 1955128

Strand: Reverse

Name: amtB [C]

Synonym: Gmet_1738

Alternate gene names: 78222947

Gene position: 1955128-1953908 (Counterclockwise)

Preceding gene: 78222958

Following gene: 78222946

Centisome position: 48.91

GC content: 59.38

Gene sequence:

>1221_bases
ATGGATCAGCTGTCGATGGCGACAGGTCTCAAGAACAGCACCGATGTCCTCTTCCTCATGCTCGGCGCCGTCATGGTCTT
TGCAATGCACGCCGGTTTCGCCTTCCTCGAAGTGGGGACAGTCCGCAAAAAGAATCAGGTCAACGCGTTTGTAAAGATCC
TGACCGACTGGTCAGTCTCCACCATCGCGTATTTCATGGTCGGCTTCCCCATCGCCTACGGCATATCCTTCCTCGTGCCG
GCCGGCGACCTCATGGGCGCCACCCAGGGGTACGACCTCGTTCACTACTTCTTCCTCCTCTGCTTTTCCGCCTGCATCCC
GGCCATAATCTCGGGAGGAATCGCCGAACGGGCCAAGTTCTGGCCCCAGGTCTTCGCCGGCGCCCTTTTCGCTGCACTGA
CCTATCCCATCTTCGAATCTCTCATCTGGGGAAAGAACGCTTCGATTCTTCAGGGGTTCTTCAAGAGTATCGGCGGCGCT
GAATTCCATGACTACGCCGGTTCGGTGGTGGTCCATTCCATGGGTGGCTGGCTTGCCCTCCCCGCAGTCCTTACCCTCGG
CCCCCGCATGGGAAGGTACATCAGGAGTAAATCCCACCCTATCCCCATCAGCAACATCCCCTTTCTGGCCCTCGGATCCT
GGATCCTGGCGGTCGGATGGTTCGGTTTCAACGTCATGAGCGCCGGCCACTTGGACAAGATATCGGGCCTCGTTGCGGTG
AACTCCCTTCTGGCCATGGTTGGTGGCGTGCTGGCGGCCCTCCTTGCAGGGAAAAACGACCCGGGTTTCATCCATAACGG
CGCCTTGGCAGGACTCATCGCCGTCTGCGCGGGCAGCGACATCATACACCCTCTGGGGGCCTTCGCTACCGGCGCTATCG
CCTCCGTTATCTTCGTCTACGGTTTCCAGATCGAGCAGGAGCGACTCAAGATCGACGACGTTCTCGGCGTCTGGCCCCTT
CACGGCATCATCGGCTCCTGGGGAGGCATTGCCGCCGGGATCTTCGGCCAGCCTCTTCTCGGCGGCATTGGTGGCATATC
ATTCATTTCCCAATTGCTTGGCACGGTCGCTGCCATCCTCTTCGCCCTTGCGAACGGGTTCCTCGTCTACACCGTTCTTT
CCAAGACCGTCGGCATCCGGCTCTCGGACGAAGAGGAGTTTGCCGGCGCTGACCTCTCCATTCACAAAATCGGCGCCTAT
CCAGAGGATCATGTGCGCTGA

Upstream 100 bases:

>100_bases
AACTGGTTAAACTATTGGCAAAGAGGTTGCTAAAGGGAAAACGGCCCTTCCCGACGACGGGAGAACGAGGGCCGAACCAC
ATGCCAACGGAGGTATGCCC

Downstream 100 bases:

>100_bases
TCTTCTTCCCTGTCACCCTTATCAAGGCCCCGTTTCGGGGCCTTTTTTACGCCGATTCGAACAGTTGAACAATTTCTTAC
TGTTTGGCTAAAGTATTTGG

Product: Rh-like protein/ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MDQLSMATGLKNSTDVLFLMLGAVMVFAMHAGFAFLEVGTVRKKNQVNAFVKILTDWSVSTIAYFMVGFPIAYGISFLVP
AGDLMGATQGYDLVHYFFLLCFSACIPAIISGGIAERAKFWPQVFAGALFAALTYPIFESLIWGKNASILQGFFKSIGGA
EFHDYAGSVVVHSMGGWLALPAVLTLGPRMGRYIRSKSHPIPISNIPFLALGSWILAVGWFGFNVMSAGHLDKISGLVAV
NSLLAMVGGVLAALLAGKNDPGFIHNGALAGLIAVCAGSDIIHPLGAFATGAIASVIFVYGFQIEQERLKIDDVLGVWPL
HGIIGSWGGIAAGIFGQPLLGGIGGISFISQLLGTVAAILFALANGFLVYTVLSKTVGIRLSDEEEFAGADLSIHKIGAY
PEDHVR

Sequences:

>Translated_406_residues
MDQLSMATGLKNSTDVLFLMLGAVMVFAMHAGFAFLEVGTVRKKNQVNAFVKILTDWSVSTIAYFMVGFPIAYGISFLVP
AGDLMGATQGYDLVHYFFLLCFSACIPAIISGGIAERAKFWPQVFAGALFAALTYPIFESLIWGKNASILQGFFKSIGGA
EFHDYAGSVVVHSMGGWLALPAVLTLGPRMGRYIRSKSHPIPISNIPFLALGSWILAVGWFGFNVMSAGHLDKISGLVAV
NSLLAMVGGVLAALLAGKNDPGFIHNGALAGLIAVCAGSDIIHPLGAFATGAIASVIFVYGFQIEQERLKIDDVLGVWPL
HGIIGSWGGIAAGIFGQPLLGGIGGISFISQLLGTVAAILFALANGFLVYTVLSKTVGIRLSDEEEFAGADLSIHKIGAY
PEDHVR
>Mature_406_residues
MDQLSMATGLKNSTDVLFLMLGAVMVFAMHAGFAFLEVGTVRKKNQVNAFVKILTDWSVSTIAYFMVGFPIAYGISFLVP
AGDLMGATQGYDLVHYFFLLCFSACIPAIISGGIAERAKFWPQVFAGALFAALTYPIFESLIWGKNASILQGFFKSIGGA
EFHDYAGSVVVHSMGGWLALPAVLTLGPRMGRYIRSKSHPIPISNIPFLALGSWILAVGWFGFNVMSAGHLDKISGLVAV
NSLLAMVGGVLAALLAGKNDPGFIHNGALAGLIAVCAGSDIIHPLGAFATGAIASVIFVYGFQIEQERLKIDDVLGVWPL
HGIIGSWGGIAAGIFGQPLLGGIGGISFISQLLGTVAAILFALANGFLVYTVLSKTVGIRLSDEEEFAGADLSIHKIGAY
PEDHVR

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=417, Percent_Identity=27.5779376498801, Blast_Score=130, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17550180, Length=438, Percent_Identity=30.1369863013699, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17550182, Length=432, Percent_Identity=28.9351851851852, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI32564042, Length=421, Percent_Identity=28.2660332541568, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI133931100, Length=443, Percent_Identity=27.765237020316, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6325396, Length=438, Percent_Identity=24.6575342465753, Blast_Score=83, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6321559, Length=440, Percent_Identity=23.6363636363636, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324187, Length=441, Percent_Identity=23.1292517006803, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI161078314, Length=418, Percent_Identity=28.9473684210526, Blast_Score=129, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 42893; Mature: 42893

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCHHHHHHHHHHHCCEECCCHHHCCCCCEEEECCCCCHHCCC
>Mature Secondary Structure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HHHHCCHHHHHHHHHHHCCEECCCHHHCCCCCEEEECCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]