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The map label for this gene is ywbI [H]

Identifier: 78222893

GI number: 78222893

Start: 1891764

End: 1892663

Strand: Direct

Name: ywbI [H]

Synonym: Gmet_1684

Alternate gene names: 78222893

Gene position: 1891764-1892663 (Clockwise)

Preceding gene: 78222892

Following gene: 78222894

Centisome position: 47.32

GC content: 54.78

Gene sequence:

>900_bases
ATGGAAAGGGTTCATGTGGATATCCGCCAGTTGCAATTTTTTGCCGAGATCGTCAGATACGGCAGCTTCACCAAGGCTGC
GGAACGGTTGCACGTAGCCCAGCCGGCAGTGAGCATGGCTATGAAGAAGTTGGAAGAGGAACTTGACCTCATACTCTTCA
ACCGTCAGGAGAGGAGAATTTCCCTGACCGCCGAGGGAGAGATCTTTCTGCGGCATGCGGAGAAGATACTGGATGAGGTG
AAGTCGGCGGAGACGGAAATGGAGGAACTGCGGGGGCTTGCCAAAGGCGAGGTGCGGGTGGGAATCCCTCCGATGATGAG
TGCATATTTCTTCCCGGAGATCATCCGTGACTTCTCCAACAGCTACCCCGATTTGCACCTTACGGTGTCGGGTGAGGGAG
CTTCAAAAATCCAGAAGATGATCATTGACGGGGAACTGGACATGGGGGTCGTTGCCGGCGCGAGCTTCCCCGAAACGCTT
GAGGTGAGAAGGTTCCTGCGGGAAGAGGTGATCGTTTGCGTCCCCAGGAGCCACCCCCTGGCGTCAAGGGGGAGCCTGAC
GCTGCACGAGTTCGCCAGGCATCCGTTGATCTTCTACCGGGAAGGGTACTATTTGAGGGAACTCATCCTTGACGTTCTGA
GGGGGGCAGGCCTCAGTCCAACCATCAGATTTGAAACAAATCTCTACACTCTGGTCAAGTCGCTGGTCCAGAAGGGTATG
GGTATTTCGGTCTTTTTGAGGATGGTGGTTGCTGAAGATGACGATCTTACGGCAATATCCCTGGATCCACCGCTGTTCCT
CGATCTTCTGATCGCCTGGAAAAAGCATGGATATCTCTCCCGGGCAAACCGCGCGTTTGCCGATTACCTCCTTGAGAAGG
GATCAAGCGCAGCACTGTAG

Upstream 100 bases:

>100_bases
GCTGATTATCCTGAGCGCAACCAACTGAACTTATTCAACTATTAACCGGACAGTAGTGAAATAAAATATATAATATTTAT
GTTCGCCATAATTAGTTTTT

Downstream 100 bases:

>100_bases
CAACCTGGACGACGTGACCGCCTCCTGCGCCGGCGGGTCGGTGATCCTTATGCCCGGGTGGTTACCTACTACCGTCCTGG
CAGCTACAGGGGGAGCATTG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MERVHVDIRQLQFFAEIVRYGSFTKAAERLHVAQPAVSMAMKKLEEELDLILFNRQERRISLTAEGEIFLRHAEKILDEV
KSAETEMEELRGLAKGEVRVGIPPMMSAYFFPEIIRDFSNSYPDLHLTVSGEGASKIQKMIIDGELDMGVVAGASFPETL
EVRRFLREEVIVCVPRSHPLASRGSLTLHEFARHPLIFYREGYYLRELILDVLRGAGLSPTIRFETNLYTLVKSLVQKGM
GISVFLRMVVAEDDDLTAISLDPPLFLDLLIAWKKHGYLSRANRAFADYLLEKGSSAAL

Sequences:

>Translated_299_residues
MERVHVDIRQLQFFAEIVRYGSFTKAAERLHVAQPAVSMAMKKLEEELDLILFNRQERRISLTAEGEIFLRHAEKILDEV
KSAETEMEELRGLAKGEVRVGIPPMMSAYFFPEIIRDFSNSYPDLHLTVSGEGASKIQKMIIDGELDMGVVAGASFPETL
EVRRFLREEVIVCVPRSHPLASRGSLTLHEFARHPLIFYREGYYLRELILDVLRGAGLSPTIRFETNLYTLVKSLVQKGM
GISVFLRMVVAEDDDLTAISLDPPLFLDLLIAWKKHGYLSRANRAFADYLLEKGSSAAL
>Mature_299_residues
MERVHVDIRQLQFFAEIVRYGSFTKAAERLHVAQPAVSMAMKKLEEELDLILFNRQERRISLTAEGEIFLRHAEKILDEV
KSAETEMEELRGLAKGEVRVGIPPMMSAYFFPEIIRDFSNSYPDLHLTVSGEGASKIQKMIIDGELDMGVVAGASFPETL
EVRRFLREEVIVCVPRSHPLASRGSLTLHEFARHPLIFYREGYYLRELILDVLRGAGLSPTIRFETNLYTLVKSLVQKGM
GISVFLRMVVAEDDDLTAISLDPPLFLDLLIAWKKHGYLSRANRAFADYLLEKGSSAAL

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=288, Percent_Identity=29.8611111111111, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1787879, Length=307, Percent_Identity=29.6416938110749, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1788481, Length=296, Percent_Identity=29.3918918918919, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787530, Length=258, Percent_Identity=25.968992248062, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1790399, Length=246, Percent_Identity=24.7967479674797, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788296, Length=282, Percent_Identity=27.3049645390071, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789204, Length=245, Percent_Identity=27.3469387755102, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI145693105, Length=289, Percent_Identity=24.5674740484429, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786401, Length=154, Percent_Identity=35.0649350649351, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788887, Length=297, Percent_Identity=24.5791245791246, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1787601, Length=238, Percent_Identity=26.890756302521, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788748, Length=291, Percent_Identity=25.085910652921, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87081904, Length=205, Percent_Identity=29.2682926829268, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1789639, Length=179, Percent_Identity=30.1675977653631, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788297, Length=241, Percent_Identity=24.0663900414938, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1790262, Length=246, Percent_Identity=24.390243902439, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI2367136, Length=247, Percent_Identity=24.2914979757085, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787806, Length=245, Percent_Identity=23.6734693877551, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786508, Length=148, Percent_Identity=27.7027027027027, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789506, Length=215, Percent_Identity=26.046511627907, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87082132, Length=254, Percent_Identity=24.8031496062992, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787949, Length=147, Percent_Identity=25.8503401360544, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789173, Length=169, Percent_Identity=28.4023668639053, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790208, Length=250, Percent_Identity=22, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33903; Mature: 33903

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERVHVDIRQLQFFAEIVRYGSFTKAAERLHVAQPAVSMAMKKLEEELDLILFNRQERRI
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEE
SLTAEGEIFLRHAEKILDEVKSAETEMEELRGLAKGEVRVGIPPMMSAYFFPEIIRDFSN
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCEEECCCHHHHHHHHHHHHHHHHC
SYPDLHLTVSGEGASKIQKMIIDGELDMGVVAGASFPETLEVRRFLREEVIVCVPRSHPL
CCCCEEEEECCCCHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHCEEEEECCCCCC
ASRGSLTLHEFARHPLIFYREGYYLRELILDVLRGAGLSPTIRFETNLYTLVKSLVQKGM
CCCCCEEHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCC
GISVFLRMVVAEDDDLTAISLDPPLFLDLLIAWKKHGYLSRANRAFADYLLEKGSSAAL
CHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MERVHVDIRQLQFFAEIVRYGSFTKAAERLHVAQPAVSMAMKKLEEELDLILFNRQERRI
CCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEE
SLTAEGEIFLRHAEKILDEVKSAETEMEELRGLAKGEVRVGIPPMMSAYFFPEIIRDFSN
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCEEECCCHHHHHHHHHHHHHHHHC
SYPDLHLTVSGEGASKIQKMIIDGELDMGVVAGASFPETLEVRRFLREEVIVCVPRSHPL
CCCCEEEEECCCCHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHCEEEEECCCCCC
ASRGSLTLHEFARHPLIFYREGYYLRELILDVLRGAGLSPTIRFETNLYTLVKSLVQKGM
CCCCCEEHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCCC
GISVFLRMVVAEDDDLTAISLDPPLFLDLLIAWKKHGYLSRANRAFADYLLEKGSSAAL
CHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]