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The map label for this gene is pfkA2 [H]

Identifier: 78222849

GI number: 78222849

Start: 1839853

End: 1840944

Strand: Direct

Name: pfkA2 [H]

Synonym: Gmet_1640

Alternate gene names: 78222849

Gene position: 1839853-1840944 (Clockwise)

Preceding gene: 78222848

Following gene: 78222850

Centisome position: 46.03

GC content: 58.79

Gene sequence:

>1092_bases
ATGAAACGGAAGATCGGCATCCTTACGGGAGGGGGAGACTGTCCCGGCCTCAACGCGGTGATACGGGGCGTGGTGAAGAG
CGCCATCATCGGGAGGGGATGGGAGGTGGTCGGTATCGAGGACGGATTCGACGGCCTTCTCTACGACCGAACCCCCAGAC
CACTGGGGCTTGAAGACGTGAGGGGAATCCTTCCGCGGGGAGGAACGATCCTTGGCACCTCCAATAGGGGCAATCCCTTT
TCCTACCCCATTGAGGTTAACGGTAAAACTGTGCTGACTGATGTGTCCGACCGGGTTGTGGAGCGGGTCAAGGACCTCGG
GATCGACGCCATCGTGGCAGTGGGGGGAGATGGTTCCCTCAAGATCGCCCTGGAGTTGATGAAAAGGGGGGTGCCGGTGG
TTGGCGTCCCGAAGACAATTGACAACGATCTAATGGAAACCGATGTCACCTTTGGTTACAACACGGCCCTGGAAACAGCC
ACTGATGCCCTCGACAAGCTCCATTCGACGGCCGAGAGCCATCACCGTATCATGATCATGGAGGTGATGGGGCGCTATGC
CGGCTGGATCGCACTGGAGTCGGGTATCAGCGGCGGTGCGGACGTGATCCTGATCCCCGAGATCCCCTTTGAACTGAAGG
CCGTCTGCAATGCCATTGAAGGCCGGCGCCGGCGAGGCAGCAAATTCAGCATTGTGGTGGCGGCCGAGGGAGCCTTTCCC
CGGGGCGGTACGCGGGTCGTCCAAAAAAAGGCCGATGAAACCATGGCAATCGAGCGGCTCGGCGGCATCGGCAACTTTGT
GGCCCACGAACTTACCTCCTGTCTCGATATGGACATCCGGGTGACAGTGCTCGGCCATCTCCAACGGGGAGGGTCCCCCT
CAACCTTTGATCGCTGTCTCGGGAGCCGATTTGGCATCGGTGCCGTTGAGCTTATCGAAAGGGAGCAGTACGGAGAGATG
ATCTGTCTCAAGGGAAGAGCCATCCGTTCCGTTCCCATTGAAAAAGCTGTCCGCAAGCTCAAACTTGTCGATCCCCGAGG
ACAGATGGTAACCGCTGCCAAAGAGCTGGGAATCAATGTCGGAAGGGAGTGA

Upstream 100 bases:

>100_bases
CGGGCAAGCGCCTCGTTATCCGCCAGGCTTCCCTGGGAGACGACGGTGGGATTCTCGGCAGTGCCGCACTGGCAAGGAGT
TTTCTTCAGGAGGGGAAACC

Downstream 100 bases:

>100_bases
GAATTGACTTTAATCCCTCCGCGCTATAGAGTGTGACGATATTTCTCTGAAGAAACTCATGCAGCGACCGATGTTATTAT
GTATGAACCTCTTTCGGGGT

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase 2; Phosphohexokinase 2 [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKRKIGILTGGGDCPGLNAVIRGVVKSAIIGRGWEVVGIEDGFDGLLYDRTPRPLGLEDVRGILPRGGTILGTSNRGNPF
SYPIEVNGKTVLTDVSDRVVERVKDLGIDAIVAVGGDGSLKIALELMKRGVPVVGVPKTIDNDLMETDVTFGYNTALETA
TDALDKLHSTAESHHRIMIMEVMGRYAGWIALESGISGGADVILIPEIPFELKAVCNAIEGRRRRGSKFSIVVAAEGAFP
RGGTRVVQKKADETMAIERLGGIGNFVAHELTSCLDMDIRVTVLGHLQRGGSPSTFDRCLGSRFGIGAVELIEREQYGEM
ICLKGRAIRSVPIEKAVRKLKLVDPRGQMVTAAKELGINVGRE

Sequences:

>Translated_363_residues
MKRKIGILTGGGDCPGLNAVIRGVVKSAIIGRGWEVVGIEDGFDGLLYDRTPRPLGLEDVRGILPRGGTILGTSNRGNPF
SYPIEVNGKTVLTDVSDRVVERVKDLGIDAIVAVGGDGSLKIALELMKRGVPVVGVPKTIDNDLMETDVTFGYNTALETA
TDALDKLHSTAESHHRIMIMEVMGRYAGWIALESGISGGADVILIPEIPFELKAVCNAIEGRRRRGSKFSIVVAAEGAFP
RGGTRVVQKKADETMAIERLGGIGNFVAHELTSCLDMDIRVTVLGHLQRGGSPSTFDRCLGSRFGIGAVELIEREQYGEM
ICLKGRAIRSVPIEKAVRKLKLVDPRGQMVTAAKELGINVGRE
>Mature_363_residues
MKRKIGILTGGGDCPGLNAVIRGVVKSAIIGRGWEVVGIEDGFDGLLYDRTPRPLGLEDVRGILPRGGTILGTSNRGNPF
SYPIEVNGKTVLTDVSDRVVERVKDLGIDAIVAVGGDGSLKIALELMKRGVPVVGVPKTIDNDLMETDVTFGYNTALETA
TDALDKLHSTAESHHRIMIMEVMGRYAGWIALESGISGGADVILIPEIPFELKAVCNAIEGRRRRGSKFSIVVAAEGAFP
RGGTRVVQKKADETMAIERLGGIGNFVAHELTSCLDMDIRVTVLGHLQRGGSPSTFDRCLGSRFGIGAVELIEREQYGEM
ICLKGRAIRSVPIEKAVRKLKLVDPRGQMVTAAKELGINVGRE

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453619, Length=335, Percent_Identity=34.3283582089552, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI266453768, Length=335, Percent_Identity=34.3283582089552, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI266453748, Length=335, Percent_Identity=34.3283582089552, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI4505749, Length=335, Percent_Identity=34.3283582089552, Blast_Score=162, Evalue=7e-40,
Organism=Homo sapiens, GI48762920, Length=372, Percent_Identity=32.5268817204301, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI11321601, Length=334, Percent_Identity=33.5329341317365, Blast_Score=154, Evalue=2e-37,
Organism=Escherichia coli, GI1790350, Length=356, Percent_Identity=37.6404494382022, Blast_Score=206, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI25147584, Length=371, Percent_Identity=30.9973045822102, Blast_Score=157, Evalue=7e-39,
Organism=Caenorhabditis elegans, GI17558788, Length=379, Percent_Identity=29.023746701847, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI72003601, Length=360, Percent_Identity=27.7777777777778, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321679, Length=342, Percent_Identity=33.9181286549708, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6323861, Length=374, Percent_Identity=30.7486631016043, Blast_Score=156, Evalue=4e-39,
Organism=Drosophila melanogaster, GI17647809, Length=369, Percent_Identity=33.3333333333333, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24652337, Length=369, Percent_Identity=33.3333333333333, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI28573326, Length=369, Percent_Identity=32.7913279132791, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 38954; Mature: 38954

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKIGILTGGGDCPGLNAVIRGVVKSAIIGRGWEVVGIEDGFDGLLYDRTPRPLGLEDV
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEECCCCCCCCHHHH
RGILPRGGTILGTSNRGNPFSYPIEVNGKTVLTDVSDRVVERVKDLGIDAIVAVGGDGSL
HHHCCCCCEEEECCCCCCCEEEEEEECCCEEEECHHHHHHHHHHHCCCEEEEEECCCCCH
KIALELMKRGVPVVGVPKTIDNDLMETDVTFGYNTALETATDALDKLHSTAESHHRIMIM
HHHHHHHHCCCCEEECCCHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEHH
EVMGRYAGWIALESGISGGADVILIPEIPFELKAVCNAIEGRRRRGSKFSIVVAAEGAFP
HHHHHHHCEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCC
RGGTRVVQKKADETMAIERLGGIGNFVAHELTSCLDMDIRVTVLGHLQRGGSPSTFDRCL
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEEHHHCCCCCHHHHHHH
GSRFGIGAVELIEREQYGEMICLKGRAIRSVPIEKAVRKLKLVDPRGQMVTAAKELGINV
CCCCCCCHHHHHHHHHCCCEEEECCCCHHCCCHHHHHHHHEEECCCCHHHHHHHHHCCCC
GRE
CCC
>Mature Secondary Structure
MKRKIGILTGGGDCPGLNAVIRGVVKSAIIGRGWEVVGIEDGFDGLLYDRTPRPLGLEDV
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEECCCCCCCCHHHH
RGILPRGGTILGTSNRGNPFSYPIEVNGKTVLTDVSDRVVERVKDLGIDAIVAVGGDGSL
HHHCCCCCEEEECCCCCCCEEEEEEECCCEEEECHHHHHHHHHHHCCCEEEEEECCCCCH
KIALELMKRGVPVVGVPKTIDNDLMETDVTFGYNTALETATDALDKLHSTAESHHRIMIM
HHHHHHHHCCCCEEECCCHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEHH
EVMGRYAGWIALESGISGGADVILIPEIPFELKAVCNAIEGRRRRGSKFSIVVAAEGAFP
HHHHHHHCEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCC
RGGTRVVQKKADETMAIERLGGIGNFVAHELTSCLDMDIRVTVLGHLQRGGSPSTFDRCL
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEEHHHCCCCCHHHHHHH
GSRFGIGAVELIEREQYGEMICLKGRAIRSVPIEKAVRKLKLVDPRGQMVTAAKELGINV
CCCCCCCHHHHHHHHHCCCEEEECCCCHHCCCHHHHHHHHEEECCCCHHHHHHHHHCCCC
GRE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]