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The map label for this gene is dut

Identifier: 78222803

GI number: 78222803

Start: 1795915

End: 1796361

Strand: Direct

Name: dut

Synonym: Gmet_1593

Alternate gene names: 78222803

Gene position: 1795915-1796361 (Clockwise)

Preceding gene: 78222802

Following gene: 78222804

Centisome position: 44.93

GC content: 58.61

Gene sequence:

>447_bases
ATGGAACGCTGCGTAGTCAAGGTTCGACGGGTGCGTGGGGGGGACCAGAATCTCCCCTGCTACATGACAGTCCACGCAGC
AGGCATGGATCTCTGTGCCGATCTCGTGGACGACCTTGTCCTGAATCCAGGTGAGCGAAAGTTGGTACCGACGGGACTTG
CTATTGCTCTCCCCGACGGATTCGAGGCCCAGATAAGGCCCCGGAGTGGTCTCGCTCTCAAGCATGGCATCGCCCTTGTG
AATTCGCCCGGCACCATTGATCCGGATTACCGTGGCGAAATAGGGGTGATCCTGATCAATCACGGCAGTGAACCGTTTGT
TGTGAGGCGGGGAGAGCGTATTGCCCAGATGGTTTTTGCCCCCTTTGCCCGTGCTGAACTGGTTGAGGTGGATGAACTCG
ACGAGACAGCGCGGGGCGAGGGAGGATTTGGCCATACCGGCCGATGA

Upstream 100 bases:

>100_bases
GCCAACGATTTCTTCTCGGGCGATTATGTCACCCTTGCCCTTACGGGGAAGATATCAGGACAGATTCCGGACCTCTCGCA
TCTCGTACTATAGGTGACTG

Downstream 100 bases:

>100_bases
AAGGAACTGAGTCCAATGTTCGATCCGCGCGTCCGTCAACTTGCCGAAGTGCTCGTTACTTACTCGACCCGGGTAAAAAA
GGGGGACGTGGTCCTCATCA

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MERCVVKVRRVRGGDQNLPCYMTVHAAGMDLCADLVDDLVLNPGERKLVPTGLAIALPDGFEAQIRPRSGLALKHGIALV
NSPGTIDPDYRGEIGVILINHGSEPFVVRRGERIAQMVFAPFARAELVEVDELDETARGEGGFGHTGR

Sequences:

>Translated_148_residues
MERCVVKVRRVRGGDQNLPCYMTVHAAGMDLCADLVDDLVLNPGERKLVPTGLAIALPDGFEAQIRPRSGLALKHGIALV
NSPGTIDPDYRGEIGVILINHGSEPFVVRRGERIAQMVFAPFARAELVEVDELDETARGEGGFGHTGR
>Mature_148_residues
MERCVVKVRRVRGGDQNLPCYMTVHAAGMDLCADLVDDLVLNPGERKLVPTGLAIALPDGFEAQIRPRSGLALKHGIALV
NSPGTIDPDYRGEIGVILINHGSEPFVVRRGERIAQMVFAPFARAELVEVDELDETARGEGGFGHTGR

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=130, Percent_Identity=45.3846153846154, Blast_Score=106, Evalue=6e-24,
Organism=Homo sapiens, GI4503423, Length=130, Percent_Identity=45.3846153846154, Blast_Score=105, Evalue=1e-23,
Organism=Homo sapiens, GI70906441, Length=130, Percent_Identity=45.3846153846154, Blast_Score=104, Evalue=3e-23,
Organism=Escherichia coli, GI1790071, Length=150, Percent_Identity=48.6666666666667, Blast_Score=131, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71988561, Length=134, Percent_Identity=41.7910447761194, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319729, Length=139, Percent_Identity=37.410071942446, Blast_Score=99, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24583610, Length=129, Percent_Identity=41.0852713178295, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19921126, Length=129, Percent_Identity=41.0852713178295, Blast_Score=94, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_GEOMG (Q39V99)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384550.1
- ProteinModelPortal:   Q39V99
- SMR:   Q39V99
- STRING:   Q39V99
- GeneID:   3741609
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1593
- NMPDR:   fig|269799.3.peg.1432
- eggNOG:   COG0756
- HOGENOM:   HBG436079
- OMA:   HGIALVN
- PhylomeDB:   Q39V99
- ProtClustDB:   PRK00601
- BioCyc:   GMET269799:GMET_1593-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16015; Mature: 16015

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: BINDING 81-81

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERCVVKVRRVRGGDQNLPCYMTVHAAGMDLCADLVDDLVLNPGERKLVPTGLAIALPDG
CCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEECCCEEEECCCC
FEAQIRPRSGLALKHGIALVNSPGTIDPDYRGEIGVILINHGSEPFVVRRGERIAQMVFA
CCEEECCCCCCEEECCEEEECCCCCCCCCCCCCEEEEEECCCCCCEEEECCHHHHHHHHC
PFARAELVEVDELDETARGEGGFGHTGR
CCCHHHEEEHHHCCHHHCCCCCCCCCCC
>Mature Secondary Structure
MERCVVKVRRVRGGDQNLPCYMTVHAAGMDLCADLVDDLVLNPGERKLVPTGLAIALPDG
CCHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEECCCEEEECCCC
FEAQIRPRSGLALKHGIALVNSPGTIDPDYRGEIGVILINHGSEPFVVRRGERIAQMVFA
CCEEECCCCCCEEECCEEEECCCCCCCCCCCCCEEEEEECCCCCCEEEECCHHHHHHHHC
PFARAELVEVDELDETARGEGGFGHTGR
CCCHHHEEEHHHCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA