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The map label for this gene is pnp

Identifier: 78222801

GI number: 78222801

Start: 1792503

End: 1794596

Strand: Direct

Name: pnp

Synonym: Gmet_1591

Alternate gene names: 78222801

Gene position: 1792503-1794596 (Clockwise)

Preceding gene: 78222800

Following gene: 78222802

Centisome position: 44.84

GC content: 56.83

Gene sequence:

>2094_bases
ATGAATGAACAGAAGGTAAACGTAGATTTTGGCGGCAGAACGATAACCATAGCTACCGGCAAAATGGCCAAACAGGCGAG
CGGTGCGGTAATGGTAAGTTGCGGTGAGACCATGGTGCTCGTCACCGCCGTGGCGCTCAAATCCGCCAAGGAGGGACAGG
ACTTCTTTCCCTTGACGGTCAACTACCAGGAAAAGGCCTATGCCGGCGGCAAGATTCCCGGCGGCTTCTTCAAACGGGAA
GGGCGCCCCACGGAGAATGAGACCCTTACCTGCCGCTTCATCGATCGGCCGATTCGTCCTCTTTTCCCTGAGAACTTTCT
CAACGATACCCAGATCATGGCCACCGTCGTTTCCGCGGATCAGGACAATGACCCGGGAATTCTTGCCATGATCGGCGCCT
CGGCGGCCCTTGAAGTATCTGATATTCCCTTTTTCGGTCCCATCGCCGGCGTAAAGGTTGGCCGGGTTGACGGGAAGTTC
ATTTGCAACCCCACTGCCGAGCAACTGGCTGCCAGTGACTTGGAAGTAGTTGTTGCAGCAAGCCGCGATGCGGTCATTAT
GGTTGAGGGTGGTGCTGCGGAGATTTCAGAAGCCGACCTCCTTGAGGCGATTTTCTTCGGCCATGCCTCGGTGCAGCCGA
TCATCGACGCCCAGATCGAACTGCGCAAGCAGGCTGGCGTACCCAAGCGGGAAATTGCTCCCCCCGCCGTCGATGAGGCT
CTTGGTGCAAAGGTCAAAGAGCTTGCGTATGCCCAGATGAAAGATGCCGTCCGGATTCGCTCGAAGCAGGAGCGCCACAA
TCGTATCAGTGCCATTGCCGATGAAACGGTGGCTGCCCTTGAAGCTGATTTTTCCGGACGCGGCGCCGAGATTAAAGATT
TCCTCGATGGTTTCGAGTATGAGCTTGTACGTGAGCACATCCTCAAAGACGGTGAGAGGATTGACGGCCGCGATACCAAA
ACGATCAGGCCCATCACTTCCGAGGTCGGTATTCTCCCTCGCGTCCACGGCTCAGCTCTTTTCACCCGTGGCGAGACCCA
GGCCCTTGTTGCCGCCACTCTCGGTACTTCCAGCGACGAACAGCGGATCGACTCACTCTACGGGGAGAGCAGAAAGAGGT
TCATGCTCCACTATAACTTCCCTCCCTTCTCTGTGGGGGAGACGAGCTTCCGTCTTGCGCCGGGCCGTCGTGAGATCGGC
CACGGTTACCTGGCTGAGCGCGCTCTTGAGCGGGTTCTGCCCAAGCACGATGATTTCCCGTACACCATCCGCATCGTGTC
GGACATTCTGGAAAGCAACGGTTCCTCCTCTATGGCTTCGGTCTGCGGCGGCGCCCTCTCCATGATGGATGCCGGCGTCC
CCATTTCCGCTCCGGTTGCAGGCATTGCCATGGGGCTCATCAAGGAAGGGGACGACATTGCCATCCTTTCCGACATCCTT
GGTGATGAAGACCACCTTGGCGACATGGACTTCAAGGTCGCCGGCACCTCGACAGGGGTGACCGCAATCCAGATGGACAT
TAAGATTACCGGCGTGACCCGTGAGATCATGGGCAAAGCCCTTGAGCAGGCAAAAGAAGGGCGGCTCCATATCCTCGGTA
AAATGGCCGAAGCCCTGGCTGCTCCGCGTACCGATCTGTCACCCTATGCCCCGCGTATCACGACTATCTGGGTCAAGACC
GACAAGATCCGTGACGTAATCGGCGCCGGTGGCAAGAACGTCAGGGGGATCACTGAGGCTACCGGCGTTTCCATCGATAT
CGAGGATACCGGCAAGATCAATATCGCCAGCACCAACAAAGAGGCCTGCGACAAGGCGATCAAGATGATCCGCGACCTTA
CGGCGGAAGCTGAAGAGGGCAAGCTTTACATGGGTACGGTCCGCAAGGTCATGGACTTTGGCGCTTTTGTCGAAATCTTC
CCGGGTACCGATGGCCTTGTTCATATCTCCGAACTCGATACCGAGCGGGTGAAGGACGTTACCGACATCCTCAAGGAAGG
GGACAAGGTGCTCGTGAAGTGCATCGGCATTGATAAGCAAGGAAAGATAAAACTGTCCCGTAAAGAAGCTCTGGGTGCAG
TACTGCCCGAGTAG

Upstream 100 bases:

>100_bases
TTTGTCAGGGTGTTGGGGGCGTGGATCGAGCAACCAAGGATGCTTGGTTTTTCTATCGACGGCCCCAACGCACCACGAAT
AGGCTAAGGAGGCCACAATT

Downstream 100 bases:

>100_bases
TGTCCGGCGGGAATCCTGCTGGTAAACTCACCGGTCCGCGGTATAGTTTTATTCATGGTCAGTAAAACAATCCTCGATAA
TGGCGTGCGGGTCATATCCG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 697; Mature: 697

Protein sequence:

>697_residues
MNEQKVNVDFGGRTITIATGKMAKQASGAVMVSCGETMVLVTAVALKSAKEGQDFFPLTVNYQEKAYAGGKIPGGFFKRE
GRPTENETLTCRFIDRPIRPLFPENFLNDTQIMATVVSADQDNDPGILAMIGASAALEVSDIPFFGPIAGVKVGRVDGKF
ICNPTAEQLAASDLEVVVAASRDAVIMVEGGAAEISEADLLEAIFFGHASVQPIIDAQIELRKQAGVPKREIAPPAVDEA
LGAKVKELAYAQMKDAVRIRSKQERHNRISAIADETVAALEADFSGRGAEIKDFLDGFEYELVREHILKDGERIDGRDTK
TIRPITSEVGILPRVHGSALFTRGETQALVAATLGTSSDEQRIDSLYGESRKRFMLHYNFPPFSVGETSFRLAPGRREIG
HGYLAERALERVLPKHDDFPYTIRIVSDILESNGSSSMASVCGGALSMMDAGVPISAPVAGIAMGLIKEGDDIAILSDIL
GDEDHLGDMDFKVAGTSTGVTAIQMDIKITGVTREIMGKALEQAKEGRLHILGKMAEALAAPRTDLSPYAPRITTIWVKT
DKIRDVIGAGGKNVRGITEATGVSIDIEDTGKINIASTNKEACDKAIKMIRDLTAEAEEGKLYMGTVRKVMDFGAFVEIF
PGTDGLVHISELDTERVKDVTDILKEGDKVLVKCIGIDKQGKIKLSRKEALGAVLPE

Sequences:

>Translated_697_residues
MNEQKVNVDFGGRTITIATGKMAKQASGAVMVSCGETMVLVTAVALKSAKEGQDFFPLTVNYQEKAYAGGKIPGGFFKRE
GRPTENETLTCRFIDRPIRPLFPENFLNDTQIMATVVSADQDNDPGILAMIGASAALEVSDIPFFGPIAGVKVGRVDGKF
ICNPTAEQLAASDLEVVVAASRDAVIMVEGGAAEISEADLLEAIFFGHASVQPIIDAQIELRKQAGVPKREIAPPAVDEA
LGAKVKELAYAQMKDAVRIRSKQERHNRISAIADETVAALEADFSGRGAEIKDFLDGFEYELVREHILKDGERIDGRDTK
TIRPITSEVGILPRVHGSALFTRGETQALVAATLGTSSDEQRIDSLYGESRKRFMLHYNFPPFSVGETSFRLAPGRREIG
HGYLAERALERVLPKHDDFPYTIRIVSDILESNGSSSMASVCGGALSMMDAGVPISAPVAGIAMGLIKEGDDIAILSDIL
GDEDHLGDMDFKVAGTSTGVTAIQMDIKITGVTREIMGKALEQAKEGRLHILGKMAEALAAPRTDLSPYAPRITTIWVKT
DKIRDVIGAGGKNVRGITEATGVSIDIEDTGKINIASTNKEACDKAIKMIRDLTAEAEEGKLYMGTVRKVMDFGAFVEIF
PGTDGLVHISELDTERVKDVTDILKEGDKVLVKCIGIDKQGKIKLSRKEALGAVLPE
>Mature_697_residues
MNEQKVNVDFGGRTITIATGKMAKQASGAVMVSCGETMVLVTAVALKSAKEGQDFFPLTVNYQEKAYAGGKIPGGFFKRE
GRPTENETLTCRFIDRPIRPLFPENFLNDTQIMATVVSADQDNDPGILAMIGASAALEVSDIPFFGPIAGVKVGRVDGKF
ICNPTAEQLAASDLEVVVAASRDAVIMVEGGAAEISEADLLEAIFFGHASVQPIIDAQIELRKQAGVPKREIAPPAVDEA
LGAKVKELAYAQMKDAVRIRSKQERHNRISAIADETVAALEADFSGRGAEIKDFLDGFEYELVREHILKDGERIDGRDTK
TIRPITSEVGILPRVHGSALFTRGETQALVAATLGTSSDEQRIDSLYGESRKRFMLHYNFPPFSVGETSFRLAPGRREIG
HGYLAERALERVLPKHDDFPYTIRIVSDILESNGSSSMASVCGGALSMMDAGVPISAPVAGIAMGLIKEGDDIAILSDIL
GDEDHLGDMDFKVAGTSTGVTAIQMDIKITGVTREIMGKALEQAKEGRLHILGKMAEALAAPRTDLSPYAPRITTIWVKT
DKIRDVIGAGGKNVRGITEATGVSIDIEDTGKINIASTNKEACDKAIKMIRDLTAEAEEGKLYMGTVRKVMDFGAFVEIF
PGTDGLVHISELDTERVKDVTDILKEGDKVLVKCIGIDKQGKIKLSRKEALGAVLPE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=710, Percent_Identity=38.3098591549296, Blast_Score=460, Evalue=1e-129,
Organism=Escherichia coli, GI145693187, Length=682, Percent_Identity=56.0117302052786, Blast_Score=763, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=718, Percent_Identity=35.933147632312, Blast_Score=381, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6320850, Length=72, Percent_Identity=44.4444444444444, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI281362905, Length=718, Percent_Identity=38.0222841225627, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24651641, Length=718, Percent_Identity=38.0222841225627, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24651643, Length=718, Percent_Identity=38.0222841225627, Blast_Score=461, Evalue=1e-130,
Organism=Drosophila melanogaster, GI161079377, Length=662, Percent_Identity=37.3111782477341, Blast_Score=416, Evalue=1e-116,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_GEOMG (Q39VA1)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384548.1
- HSSP:   Q9NP64
- ProteinModelPortal:   Q39VA1
- SMR:   Q39VA1
- STRING:   Q39VA1
- GeneID:   3741607
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1591
- NMPDR:   fig|269799.3.peg.1430
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   Q39VA1
- ProtClustDB:   PRK11824
- BioCyc:   GMET269799:GMET_1591-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 75052; Mature: 75052

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEQKVNVDFGGRTITIATGKMAKQASGAVMVSCGETMVLVTAVALKSAKEGQDFFPLTV
CCCCEEEEEECCEEEEEECCCHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEE
NYQEKAYAGGKIPGGFFKREGRPTENETLTCRFIDRPIRPLFPENFLNDTQIMATVVSAD
CCCHHHCCCCCCCCCHHCCCCCCCCCCEEEEEEECCCCCCCCCHHHCCCHHHHHEEEECC
QDNDPGILAMIGASAALEVSDIPFFGPIAGVKVGRVDGKFICNPTAEQLAASDLEVVVAA
CCCCCCEEEEECCCCEEEECCCCCCCCCCCEEEECCCCEEEECCCHHHHHHCCCEEEEEE
SRDAVIMVEGGAAEISEADLLEAIFFGHASVQPIIDAQIELRKQAGVPKREIAPPAVDEA
CCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHCCCCHHHHH
LGAKVKELAYAQMKDAVRIRSKQERHNRISAIADETVAALEADFSGRGAEIKDFLDGFEY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHH
ELVREHILKDGERIDGRDTKTIRPITSEVGILPRVHGSALFTRGETQALVAATLGTSSDE
HHHHHHHHCCCCCCCCCCCCEECCCHHHCCCCCCCCCCEEEECCCCCEEEEEECCCCCHH
QRIDSLYGESRKRFMLHYNFPPFSVGETSFRLAPGRREIGHGYLAERALERVLPKHDDFP
HHHHHHHCCCCEEEEEEECCCCCCCCCCCEEECCCCHHHCCHHHHHHHHHHHCCCCCCCC
YTIRIVSDILESNGSSSMASVCGGALSMMDAGVPISAPVAGIAMGLIKEGDDIAILSDIL
EEHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEHHHHC
GDEDHLGDMDFKVAGTSTGVTAIQMDIKITGVTREIMGKALEQAKEGRLHILGKMAEALA
CCCCCCCCCCEEEECCCCCEEEEEEEEEEECCHHHHHHHHHHHHHCCCEEHHHHHHHHHH
APRTDLSPYAPRITTIWVKTDKIRDVIGAGGKNVRGITEATGVSIDIEDTGKINIASTNK
CCCCCCCCCCCCEEEEEEECHHHHHHHCCCCCCCCCCCHHCCCEEEECCCCEEEEECCCH
EACDKAIKMIRDLTAEAEEGKLYMGTVRKVMDFGAFVEIFPGTDGLVHISELDTERVKDV
HHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCEEEEECCCCCEEEEHHCCHHHHHHH
TDILKEGDKVLVKCIGIDKQGKIKLSRKEALGAVLPE
HHHHHCCCEEEEEEECCCCCCCEEEEHHHHCCCCCCC
>Mature Secondary Structure
MNEQKVNVDFGGRTITIATGKMAKQASGAVMVSCGETMVLVTAVALKSAKEGQDFFPLTV
CCCCEEEEEECCEEEEEECCCHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEE
NYQEKAYAGGKIPGGFFKREGRPTENETLTCRFIDRPIRPLFPENFLNDTQIMATVVSAD
CCCHHHCCCCCCCCCHHCCCCCCCCCCEEEEEEECCCCCCCCCHHHCCCHHHHHEEEECC
QDNDPGILAMIGASAALEVSDIPFFGPIAGVKVGRVDGKFICNPTAEQLAASDLEVVVAA
CCCCCCEEEEECCCCEEEECCCCCCCCCCCEEEECCCCEEEECCCHHHHHHCCCEEEEEE
SRDAVIMVEGGAAEISEADLLEAIFFGHASVQPIIDAQIELRKQAGVPKREIAPPAVDEA
CCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHCCCCHHHHH
LGAKVKELAYAQMKDAVRIRSKQERHNRISAIADETVAALEADFSGRGAEIKDFLDGFEY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHH
ELVREHILKDGERIDGRDTKTIRPITSEVGILPRVHGSALFTRGETQALVAATLGTSSDE
HHHHHHHHCCCCCCCCCCCCEECCCHHHCCCCCCCCCCEEEECCCCCEEEEEECCCCCHH
QRIDSLYGESRKRFMLHYNFPPFSVGETSFRLAPGRREIGHGYLAERALERVLPKHDDFP
HHHHHHHCCCCEEEEEEECCCCCCCCCCCEEECCCCHHHCCHHHHHHHHHHHCCCCCCCC
YTIRIVSDILESNGSSSMASVCGGALSMMDAGVPISAPVAGIAMGLIKEGDDIAILSDIL
EEHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEHHHHC
GDEDHLGDMDFKVAGTSTGVTAIQMDIKITGVTREIMGKALEQAKEGRLHILGKMAEALA
CCCCCCCCCCEEEECCCCCEEEEEEEEEEECCHHHHHHHHHHHHHCCCEEHHHHHHHHHH
APRTDLSPYAPRITTIWVKTDKIRDVIGAGGKNVRGITEATGVSIDIEDTGKINIASTNK
CCCCCCCCCCCCEEEEEEECHHHHHHHCCCCCCCCCCCHHCCCEEEECCCCEEEEECCCH
EACDKAIKMIRDLTAEAEEGKLYMGTVRKVMDFGAFVEIFPGTDGLVHISELDTERVKDV
HHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCEEEEECCCCCEEEEHHCCHHHHHHH
TDILKEGDKVLVKCIGIDKQGKIKLSRKEALGAVLPE
HHHHHCCCEEEEEEECCCCCCCEEEEHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA