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The map label for this gene is truB

Identifier: 78222799

GI number: 78222799

Start: 1790972

End: 1791886

Strand: Direct

Name: truB

Synonym: Gmet_1589

Alternate gene names: 78222799

Gene position: 1790972-1791886 (Clockwise)

Preceding gene: 78222798

Following gene: 78222800

Centisome position: 44.8

GC content: 56.5

Gene sequence:

>915_bases
ATGGACGGTTTCATTGTTATCGACAAGCCGGCCGGCTTGACTTCCCACGACATCATTGCGCGCGTCAGACGGATTCTCGG
CCAGAAGAAGGCGGGCCATACGGGAACCCTTGATCCCTTTGCCACCGGAGTTTTGCCGGTTGCAATGGGAGAGGGGACCA
AGGCGATACCGTTTCTTGATGAGGCTGTGAAAGAGTACCGTGCAACCATGGTGCTCGGTGCTGCCACCGATACCCAGGAC
TGTACCGGCAGGGTCGAGAAAGAAGGGGACTGGACTCGATTGTCCGTTGAGGCGGTCAGGGAAGTTTTCGGTGCATATAT
CGGAAAGTGCAAGCAGATCCCTCCCATGTACTCTGCGGTCAAGCAAGGGGGGGTTCCCCTCTACCGACTAGCGCGAAAAG
GGCATGAGGTGGAACGAGAACCCCGGGATATCGAGATCCATTCCCTCATAATCGATCGTATGGAGCTGCCGCGAATTGAG
TTTACGGTTTCCTGCTCCCGGGGCACCTATGTGAGAACGCTGGCACACGACATGGGGGAGCGCCTCGGTTGCGGCGCCCA
TCTTTCTGCGCTGCGCAGAACGCGGAGCGGCCCGTTTGACCTTTCCCGGGCCGTGACCCTTGACGAACTGGCAAGCATCA
AGGAACAAGGCGCTGTTGATTCGCTTCTGCTGGCTCCCAACGAGGTGCTTGGTCATTTTCCCGAGATCGAGCTCTCAGAC
GAGGAAGTCCGCAAGGTTTACTGCGGAGTCCCTCCCCGTTGCCATGGCAGATGCGAAGAATCTTCAGCGTCGCAAAATGA
CGGTAAGAAGGTGCGTCTCGTTCAGAATGGCAAGCTTCTCGCTGTGGCTGAAATCGGTCAGGCTGAATCGGGTAACGGGA
ACAAAAGCCTAAGGCTTATAAGGGTTTTTAACTAG

Upstream 100 bases:

>100_bases
GGCGGCGGGCATCACAATGCCGCAGGAGCTACGATGAATGGTTCCCTTGAAGGGATAAAGGCAACGTTGTTCTCCCACCT
GGAGAGTACCCTCTGATCTG

Downstream 100 bases:

>100_bases
GTTTGGCTTTACAGGGGCGGTTCATTGTGGTATTAGTGTCCCTTGAGTTTTTTTGAAGCACATCACAAACGATCACAGAT
CGGAACACATGAAGATGAAA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MDGFIVIDKPAGLTSHDIIARVRRILGQKKAGHTGTLDPFATGVLPVAMGEGTKAIPFLDEAVKEYRATMVLGAATDTQD
CTGRVEKEGDWTRLSVEAVREVFGAYIGKCKQIPPMYSAVKQGGVPLYRLARKGHEVEREPRDIEIHSLIIDRMELPRIE
FTVSCSRGTYVRTLAHDMGERLGCGAHLSALRRTRSGPFDLSRAVTLDELASIKEQGAVDSLLLAPNEVLGHFPEIELSD
EEVRKVYCGVPPRCHGRCEESSASQNDGKKVRLVQNGKLLAVAEIGQAESGNGNKSLRLIRVFN

Sequences:

>Translated_304_residues
MDGFIVIDKPAGLTSHDIIARVRRILGQKKAGHTGTLDPFATGVLPVAMGEGTKAIPFLDEAVKEYRATMVLGAATDTQD
CTGRVEKEGDWTRLSVEAVREVFGAYIGKCKQIPPMYSAVKQGGVPLYRLARKGHEVEREPRDIEIHSLIIDRMELPRIE
FTVSCSRGTYVRTLAHDMGERLGCGAHLSALRRTRSGPFDLSRAVTLDELASIKEQGAVDSLLLAPNEVLGHFPEIELSD
EEVRKVYCGVPPRCHGRCEESSASQNDGKKVRLVQNGKLLAVAEIGQAESGNGNKSLRLIRVFN
>Mature_304_residues
MDGFIVIDKPAGLTSHDIIARVRRILGQKKAGHTGTLDPFATGVLPVAMGEGTKAIPFLDEAVKEYRATMVLGAATDTQD
CTGRVEKEGDWTRLSVEAVREVFGAYIGKCKQIPPMYSAVKQGGVPLYRLARKGHEVEREPRDIEIHSLIIDRMELPRIE
FTVSCSRGTYVRTLAHDMGERLGCGAHLSALRRTRSGPFDLSRAVTLDELASIKEQGAVDSLLLAPNEVLGHFPEIELSD
EEVRKVYCGVPPRCHGRCEESSASQNDGKKVRLVQNGKLLAVAEIGQAESGNGNKSLRLIRVFN

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=251, Percent_Identity=32.2709163346614, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI215599015, Length=196, Percent_Identity=36.2244897959184, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI4503337, Length=196, Percent_Identity=36.2244897959184, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI2367200, Length=294, Percent_Identity=37.0748299319728, Blast_Score=191, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI17553978, Length=198, Percent_Identity=31.8181818181818, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324037, Length=198, Percent_Identity=29.7979797979798, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323204, Length=289, Percent_Identity=28.0276816608997, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI281364189, Length=197, Percent_Identity=33.502538071066, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI281364187, Length=197, Percent_Identity=33.502538071066, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI281364185, Length=197, Percent_Identity=33.502538071066, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI281364183, Length=197, Percent_Identity=33.502538071066, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI62471759, Length=197, Percent_Identity=33.502538071066, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI17975520, Length=197, Percent_Identity=33.502538071066, Blast_Score=91, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_GEOMG (Q39VA3)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384546.1
- ProteinModelPortal:   Q39VA3
- SMR:   Q39VA3
- STRING:   Q39VA3
- GeneID:   3740570
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1589
- NMPDR:   fig|269799.3.peg.1428
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- PhylomeDB:   Q39VA3
- ProtClustDB:   CLSK2522239
- BioCyc:   GMET269799:GMET_1589-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 33274; Mature: 33274

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: NA

Important sites: ACT_SITE 38-38

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGFIVIDKPAGLTSHDIIARVRRILGQKKAGHTGTLDPFATGVLPVAMGEGTKAIPFLD
CCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCEEEEECCCCCCCHHHH
EAVKEYRATMVLGAATDTQDCTGRVEKEGDWTRLSVEAVREVFGAYIGKCKQIPPMYSAV
HHHHHHHHHEEEECCCCCHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCHHHHHH
KQGGVPLYRLARKGHEVEREPRDIEIHSLIIDRMELPRIEFTVSCSRGTYVRTLAHDMGE
HHCCCHHHHHHHCCCCCCCCCCCEEHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHH
RLGCGAHLSALRRTRSGPFDLSRAVTLDELASIKEQGAVDSLLLAPNEVLGHFPEIELSD
HCCCCHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHCCCHHHHHCCCHHHHCCCCCCCCCH
EEVRKVYCGVPPRCHGRCEESSASQNDGKKVRLVQNGKLLAVAEIGQAESGNGNKSLRLI
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCEEEEE
RVFN
EECC
>Mature Secondary Structure
MDGFIVIDKPAGLTSHDIIARVRRILGQKKAGHTGTLDPFATGVLPVAMGEGTKAIPFLD
CCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCEEEEECCCCCCCHHHH
EAVKEYRATMVLGAATDTQDCTGRVEKEGDWTRLSVEAVREVFGAYIGKCKQIPPMYSAV
HHHHHHHHHEEEECCCCCHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCHHHHHH
KQGGVPLYRLARKGHEVEREPRDIEIHSLIIDRMELPRIEFTVSCSRGTYVRTLAHDMGE
HHCCCHHHHHHHCCCCCCCCCCCEEHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHH
RLGCGAHLSALRRTRSGPFDLSRAVTLDELASIKEQGAVDSLLLAPNEVLGHFPEIELSD
HCCCCHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHCCCHHHHHCCCHHHHCCCCCCCCCH
EEVRKVYCGVPPRCHGRCEESSASQNDGKKVRLVQNGKLLAVAEIGQAESGNGNKSLRLI
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCEEEEE
RVFN
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA