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The map label for this gene is mdeA [H]

Identifier: 78222776

GI number: 78222776

Start: 1768827

End: 1770071

Strand: Direct

Name: mdeA [H]

Synonym: Gmet_1566

Alternate gene names: 78222776

Gene position: 1768827-1770071 (Clockwise)

Preceding gene: 304570574

Following gene: 78222777

Centisome position: 44.25

GC content: 63.69

Gene sequence:

>1245_bases
ATGGCGCGGGATTTTCAGCTCGACACCCTCTTGATTCACGGCGGCCTTGAACCCGGCCCTGCTGGGGCCACGACGGTTCC
CATCGTCCAGTCTTCCTCCTTTGCCCACGAAACCGCCGAGGATCTGGAGGACATCTTCCGGGGCCGCAAGGCGGGGCATG
TCTATACCCGGATCAACAACCCGACCACCGAGAGTCTGGAGCGCCGGTTGGCGCTGCTGGAAGGGGGGATCTCGGCCATT
GCCACCTCCTCGGGGATGGCGGCCATCACTCTGGCGGTGCTGTCCATCGTCCGCTGCGGCGACGAGATCCTCTCCTCGGC
ATCCCTCTTCGGCGGCACCTTCTCCCTCTTCCGCGACACCCTCTCCAACTACGGCATCGCCACCCGTTTCGTGGATCCGA
GTGATCTCGCCGCAATGCGGGCCGCAGTCACCGAGCGAACCCGTCTCATCTTTGTGGAGACCATGGGGAACCCGCGGCTC
GACGTCCCCGACCTGGCGGCCCTGGCTGCGATCGCCCGGGAGGCAGGTGTGCCCCTCGTAGTGGACGCCACCGTCACCAC
GCCAGTACTCGGCAGGGTGAAAGAGCTGGGGGCCGACATTATTGTCCACTCCACCAGCAAGTACATCAACGGCACCGGCA
GCGCCATCGGCGGGGCCATCATCGACTGCGGCACATTTGACTGGCGGCGGGAGAGCTATCCCCACTTCGAGCCGTACGTG
AAGAAGTTTCGCCAGTTCGCCTTCTCCGCCCGTGCCCGAAAGTTGATCCACAAAGACCTCGGCGCCTGTCCCGCTCCTAT
GAACTCATACCTTCTGTCGGAAGGGATCGAAACCATGGCGCTGCGGATGGAGCGCAACTGCGCAAATGCCCAGCGCCTGG
CGGAGTTCCTGGCCGCCCACCCCAAGGTGGCGTGGGTCAACTATCCCGGCCTCACCAGTTCGCCGTGGCACGAAACGGCT
AAAGCGCAGTTCGGCGGCCGCTACGGCGCGCTTCTGACCTTCGGCCTTGCTGACAAGGCTGCTGCCTTCCACTGCATCGA
CGGCCTGCGGCTGGCCAAGAATCTGGCCAACATCGGCGACACCAAGACTCTGGTGATTCATCCCGCCAGCACCATCTGCG
CCGATTTCGGCCCCGAGGAGAAGGCTCTCATGGGAGTGAGCGAGGAGATGATCCGCGTCTCCGTCGGCATCGAGGCCATC
GACGACATAATCGAGGACTTTGCGGAGTCCCTCAACGCTATATAG

Upstream 100 bases:

>100_bases
TGTTGACATAATTGGTGATAAAAAGGCGCTGTTATTTTGCGGCTGGTTCGATGTGACTTACTTCGCGTGGCCGGGAATGA
TGGCAAGGGGAAAAACGAAT

Downstream 100 bases:

>100_bases
AAGGAGATTGCAATGAATTTGACGGTAAACGGTAAACCCTCCACGGTGGATGGTGCAGAGAGCCTGAACGTGACCGAACT
CCTCTCGGCGCTCAAGGTGG

Product: Cys/Met metabolism pyridoxal-phosphate-dependent enzyme

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MARDFQLDTLLIHGGLEPGPAGATTVPIVQSSSFAHETAEDLEDIFRGRKAGHVYTRINNPTTESLERRLALLEGGISAI
ATSSGMAAITLAVLSIVRCGDEILSSASLFGGTFSLFRDTLSNYGIATRFVDPSDLAAMRAAVTERTRLIFVETMGNPRL
DVPDLAALAAIAREAGVPLVVDATVTTPVLGRVKELGADIIVHSTSKYINGTGSAIGGAIIDCGTFDWRRESYPHFEPYV
KKFRQFAFSARARKLIHKDLGACPAPMNSYLLSEGIETMALRMERNCANAQRLAEFLAAHPKVAWVNYPGLTSSPWHETA
KAQFGGRYGALLTFGLADKAAAFHCIDGLRLAKNLANIGDTKTLVIHPASTICADFGPEEKALMGVSEEMIRVSVGIEAI
DDIIEDFAESLNAI

Sequences:

>Translated_414_residues
MARDFQLDTLLIHGGLEPGPAGATTVPIVQSSSFAHETAEDLEDIFRGRKAGHVYTRINNPTTESLERRLALLEGGISAI
ATSSGMAAITLAVLSIVRCGDEILSSASLFGGTFSLFRDTLSNYGIATRFVDPSDLAAMRAAVTERTRLIFVETMGNPRL
DVPDLAALAAIAREAGVPLVVDATVTTPVLGRVKELGADIIVHSTSKYINGTGSAIGGAIIDCGTFDWRRESYPHFEPYV
KKFRQFAFSARARKLIHKDLGACPAPMNSYLLSEGIETMALRMERNCANAQRLAEFLAAHPKVAWVNYPGLTSSPWHETA
KAQFGGRYGALLTFGLADKAAAFHCIDGLRLAKNLANIGDTKTLVIHPASTICADFGPEEKALMGVSEEMIRVSVGIEAI
DDIIEDFAESLNAI
>Mature_413_residues
ARDFQLDTLLIHGGLEPGPAGATTVPIVQSSSFAHETAEDLEDIFRGRKAGHVYTRINNPTTESLERRLALLEGGISAIA
TSSGMAAITLAVLSIVRCGDEILSSASLFGGTFSLFRDTLSNYGIATRFVDPSDLAAMRAAVTERTRLIFVETMGNPRLD
VPDLAALAAIAREAGVPLVVDATVTTPVLGRVKELGADIIVHSTSKYINGTGSAIGGAIIDCGTFDWRRESYPHFEPYVK
KFRQFAFSARARKLIHKDLGACPAPMNSYLLSEGIETMALRMERNCANAQRLAEFLAAHPKVAWVNYPGLTSSPWHETAK
AQFGGRYGALLTFGLADKAAAFHCIDGLRLAKNLANIGDTKTLVIHPASTICADFGPEEKALMGVSEEMIRVSVGIEAID
DIIEDFAESLNAI

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=395, Percent_Identity=31.8987341772152, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI299473759, Length=395, Percent_Identity=29.1139240506329, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI25453487, Length=394, Percent_Identity=27.9187817258883, Blast_Score=142, Evalue=5e-34,
Organism=Escherichia coli, GI1790375, Length=407, Percent_Identity=33.1695331695332, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI1789383, Length=412, Percent_Identity=26.2135922330097, Blast_Score=132, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI71980708, Length=404, Percent_Identity=30.9405940594059, Blast_Score=197, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI17538055, Length=405, Percent_Identity=30.6172839506173, Blast_Score=184, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17560056, Length=392, Percent_Identity=30.3571428571429, Blast_Score=172, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6323333, Length=435, Percent_Identity=39.5402298850575, Blast_Score=288, Evalue=1e-78,
Organism=Saccharomyces cerevisiae, GI6319307, Length=414, Percent_Identity=30.6763285024155, Blast_Score=163, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6321170, Length=309, Percent_Identity=25.2427184466019, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321904, Length=425, Percent_Identity=25.6470588235294, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6321254, Length=430, Percent_Identity=23.2558139534884, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6322970, Length=326, Percent_Identity=25.7668711656442, Blast_Score=73, Evalue=8e-14,
Organism=Drosophila melanogaster, GI19922554, Length=408, Percent_Identity=28.921568627451, Blast_Score=179, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 44456; Mature: 44325

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDFQLDTLLIHGGLEPGPAGATTVPIVQSSSFAHETAEDLEDIFRGRKAGHVYTRINN
CCCCCEEEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEECCC
PTTESLERRLALLEGGISAIATSSGMAAITLAVLSIVRCGDEILSSASLFGGTFSLFRDT
CCHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LSNYGIATRFVDPSDLAAMRAAVTERTRLIFVETMGNPRLDVPDLAALAAIAREAGVPLV
HHCCCCEEEECCHHHHHHHHHHHHHCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEE
VDATVTTPVLGRVKELGADIIVHSTSKYINGTGSAIGGAIIDCGTFDWRRESYPHFEPYV
EECCHHHHHHHHHHHCCCCEEEECCHHHCCCCCCHHCCCEEECCCCCCCCCCCCCHHHHH
KKFRQFAFSARARKLIHKDLGACPAPMNSYLLSEGIETMALRMERNCANAQRLAEFLAAH
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
PKVAWVNYPGLTSSPWHETAKAQFGGRYGALLTFGLADKAAAFHCIDGLRLAKNLANIGD
CCEEEEECCCCCCCCCHHHHHHHCCCCEEHHEEECCHHHHHHHHHHHHHHHHHHHHHCCC
TKTLVIHPASTICADFGPEEKALMGVSEEMIRVSVGIEAIDDIIEDFAESLNAI
CCEEEEECHHHHHHCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARDFQLDTLLIHGGLEPGPAGATTVPIVQSSSFAHETAEDLEDIFRGRKAGHVYTRINN
CCCCEEEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEECCC
PTTESLERRLALLEGGISAIATSSGMAAITLAVLSIVRCGDEILSSASLFGGTFSLFRDT
CCHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LSNYGIATRFVDPSDLAAMRAAVTERTRLIFVETMGNPRLDVPDLAALAAIAREAGVPLV
HHCCCCEEEECCHHHHHHHHHHHHHCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEE
VDATVTTPVLGRVKELGADIIVHSTSKYINGTGSAIGGAIIDCGTFDWRRESYPHFEPYV
EECCHHHHHHHHHHHCCCCEEEECCHHHCCCCCCHHCCCEEECCCCCCCCCCCCCHHHHH
KKFRQFAFSARARKLIHKDLGACPAPMNSYLLSEGIETMALRMERNCANAQRLAEFLAAH
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
PKVAWVNYPGLTSSPWHETAKAQFGGRYGALLTFGLADKAAAFHCIDGLRLAKNLANIGD
CCEEEEECCCCCCCCCHHHHHHHCCCCEEHHEEECCHHHHHHHHHHHHHHHHHHHHHCCC
TKTLVIHPASTICADFGPEEKALMGVSEEMIRVSVGIEAIDDIIEDFAESLNAI
CCEEEEECHHHHHHCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]