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The map label for this gene is pflC [H]

Identifier: 78222753

GI number: 78222753

Start: 1745289

End: 1746335

Strand: Reverse

Name: pflC [H]

Synonym: Gmet_1541

Alternate gene names: 78222753

Gene position: 1746335-1745289 (Counterclockwise)

Preceding gene: 78222755

Following gene: 78222752

Centisome position: 43.69

GC content: 50.33

Gene sequence:

>1047_bases
ATGCTCACTCCACTCATCACCGAAATTCAAAGATTCTGCCTCCATGACGGCCCCGGCATCAGAACTACCATCTTCGTCAA
GGGCTGCCCTCTCCATTGCCCATGGTGCCATAATCCTGAGAACATAAATCCTAAGCAAGAGTTCTATTATCATGCAAGCA
AATGCAGTAATTGCGGACAGTGTCTGACAGCTTGTCCATCGGGTGTTGATAATCCTGAGCATGACGACTGTATTGGGAAA
ACGACCGATAGATCTCATTGCACATCATGTTTCCAGTGTGTATCTGCCTGCCGGTTTGGCGCACGTGAAACAGTAGGCAA
ATTCATTTCGATAGAAAGCATTGTCCAGGAGGCTGTGTCCGATCGAATCTTTTACCAGCATAGCGGAGGTGGTGTCACGG
TCAGTGGCGGAGAGCCTCTGATGTATCCTGAATTCACCCGTGATCTGACGTATAGACTGAAAGTAAAAGAGAATGTTCAT
GTAGCGATAGAGACATCTCTGTTTGCCGAATGGCATAATATTGAACCCCTTCTGAAGTACGTAGATCTTTTTATTGTCGA
CATCAAAACCCCTGATCCCCAAAAGCATCAACACGTTATCGGTGGTTCCCTGCACAAAATCTTATCGAACCTGGAACGGC
TCCTGGAAGCCAGGGCAACAGTACGTACCCATCTCCCGATCATTCCCGGCATCAACGACACCAGTCAAGATTTCGAAGCC
TATGCAGAGTATCTGGGGCAGTTCGCCAACCAACTGTCCGGAGTCGACATCCTTCCCTATCACTCCTATGCGACGGGGAA
ATATGTGCAACTGGGGCGCAGCTATCAATACCTGGGGGTTCCCGACCTTCCGGCACAGCAGCTTACCCCCCTGGTTAACG
CACTCAGGCAGCAAGGAATTCGAGAGATTACCCTCGGCGGTATGGTTGGGTCGTCACCAGCAGTTGAAAATGTTGCCGGC
ACGAGATCGCTGAAGCCAAGGCGGGATTATTTTTCACGCCCGGTTTATTCGCCGCAGGGAAGGGGGGTGGTGGTCACTCA
ACGCTAA

Upstream 100 bases:

>100_bases
TGTCTTATGAAACACTGTTTTGGCACAACTGCTGCTAATGCTCTACTAGAAAGCACGTTCGGTTCAGCAGCATACCCTTT
GTCAATCAGGGAGGTGCACC

Downstream 100 bases:

>100_bases
TCGCGCTGTATCACGACAAATCCGCAGGATGTCAACCATGCACATAAAGGAGATTCGAAATGACCACTTGTAAGAATTGC
TCATTCTATTTTGCCGTTCC

Product: glycyl-radical activating protein

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 2; PFL-activating enzyme 2 [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MLTPLITEIQRFCLHDGPGIRTTIFVKGCPLHCPWCHNPENINPKQEFYYHASKCSNCGQCLTACPSGVDNPEHDDCIGK
TTDRSHCTSCFQCVSACRFGARETVGKFISIESIVQEAVSDRIFYQHSGGGVTVSGGEPLMYPEFTRDLTYRLKVKENVH
VAIETSLFAEWHNIEPLLKYVDLFIVDIKTPDPQKHQHVIGGSLHKILSNLERLLEARATVRTHLPIIPGINDTSQDFEA
YAEYLGQFANQLSGVDILPYHSYATGKYVQLGRSYQYLGVPDLPAQQLTPLVNALRQQGIREITLGGMVGSSPAVENVAG
TRSLKPRRDYFSRPVYSPQGRGVVVTQR

Sequences:

>Translated_348_residues
MLTPLITEIQRFCLHDGPGIRTTIFVKGCPLHCPWCHNPENINPKQEFYYHASKCSNCGQCLTACPSGVDNPEHDDCIGK
TTDRSHCTSCFQCVSACRFGARETVGKFISIESIVQEAVSDRIFYQHSGGGVTVSGGEPLMYPEFTRDLTYRLKVKENVH
VAIETSLFAEWHNIEPLLKYVDLFIVDIKTPDPQKHQHVIGGSLHKILSNLERLLEARATVRTHLPIIPGINDTSQDFEA
YAEYLGQFANQLSGVDILPYHSYATGKYVQLGRSYQYLGVPDLPAQQLTPLVNALRQQGIREITLGGMVGSSPAVENVAG
TRSLKPRRDYFSRPVYSPQGRGVVVTQR
>Mature_348_residues
MLTPLITEIQRFCLHDGPGIRTTIFVKGCPLHCPWCHNPENINPKQEFYYHASKCSNCGQCLTACPSGVDNPEHDDCIGK
TTDRSHCTSCFQCVSACRFGARETVGKFISIESIVQEAVSDRIFYQHSGGGVTVSGGEPLMYPEFTRDLTYRLKVKENVH
VAIETSLFAEWHNIEPLLKYVDLFIVDIKTPDPQKHQHVIGGSLHKILSNLERLLEARATVRTHLPIIPGINDTSQDFEA
YAEYLGQFANQLSGVDILPYHSYATGKYVQLGRSYQYLGVPDLPAQQLTPLVNALRQQGIREITLGGMVGSSPAVENVAG
TRSLKPRRDYFSRPVYSPQGRGVVVTQR

Specific function: Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI1790389, Length=302, Percent_Identity=32.7814569536424, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI226510931, Length=273, Percent_Identity=33.6996336996337, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1787130, Length=199, Percent_Identity=31.6582914572864, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790839, Length=302, Percent_Identity=23.5099337748344, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006638
- InterPro:   IPR012839
- InterPro:   IPR011352
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 38795; Mature: 38795

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: PS01087 RADICAL_ACTIVATING ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTPLITEIQRFCLHDGPGIRTTIFVKGCPLHCPWCHNPENINPKQEFYYHASKCSNCGQ
CCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHEEHHHHCCHHH
CLTACPSGVDNPEHDDCIGKTTDRSHCTSCFQCVSACRFGARETVGKFISIESIVQEAVS
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DRIFYQHSGGGVTVSGGEPLMYPEFTRDLTYRLKVKENVHVAIETSLFAEWHNIEPLLKY
HCEEEEECCCEEEECCCCCCCCCHHHCCEEEEEEEECCEEEEEEHHHHHHHCCHHHHHHH
VDLFIVDIKTPDPQKHQHVIGGSLHKILSNLERLLEARATVRTHLPIIPGINDTSQDFEA
HHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
YAEYLGQFANQLSGVDILPYHSYATGKYVQLGRSYQYLGVPDLPAQQLTPLVNALRQQGI
HHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCC
REITLGGMVGSSPAVENVAGTRSLKPRRDYFSRPVYSPQGRGVVVTQR
CEEEECCCCCCCCCHHHHCCCCCCCCHHHHHCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MLTPLITEIQRFCLHDGPGIRTTIFVKGCPLHCPWCHNPENINPKQEFYYHASKCSNCGQ
CCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHEEHHHHCCHHH
CLTACPSGVDNPEHDDCIGKTTDRSHCTSCFQCVSACRFGARETVGKFISIESIVQEAVS
HHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
DRIFYQHSGGGVTVSGGEPLMYPEFTRDLTYRLKVKENVHVAIETSLFAEWHNIEPLLKY
HCEEEEECCCEEEECCCCCCCCCHHHCCEEEEEEEECCEEEEEEHHHHHHHCCHHHHHHH
VDLFIVDIKTPDPQKHQHVIGGSLHKILSNLERLLEARATVRTHLPIIPGINDTSQDFEA
HHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
YAEYLGQFANQLSGVDILPYHSYATGKYVQLGRSYQYLGVPDLPAQQLTPLVNALRQQGI
HHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCC
REITLGGMVGSSPAVENVAGTRSLKPRRDYFSRPVYSPQGRGVVVTQR
CEEEECCCCCCCCCHHHHCCCCCCCCHHHHHCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]