Click here to switch to the map view.

The map label for this gene is glnG [H]

Identifier: 78222435

GI number: 78222435

Start: 1376613

End: 1377044

Strand: Reverse

Name: glnG [H]

Synonym: Gmet_1220

Alternate gene names: 78222435

Gene position: 1377044-1376613 (Counterclockwise)

Preceding gene: 78222443

Following gene: 78222429

Centisome position: 34.45

GC content: 56.25

Gene sequence:

>432_bases
ATGAACCCGACTCAAGGGCACAGGACTGCCACTATCCGGCCAACACGCATCCTCGTCGTTGACGATGACCGCGATGTCCT
CTTTTTCATGAAACTGGCCCTGGAAACCGAGGGGATAGACGTCACATGCGCCGAAAGGGGCGAAGATGCCCTCGGACTGC
TTGCAGAAAGGTCCTATGGAGTCCTTCTCACCGACCAGAACATGCCGGGGATGGATGGATTTGAACTGGCCCAGCACGCC
AGGCGCCTCCAACCGGAGCTGATAATATTCATGGGAACCGGACACCTCTACCCGGGAATCGAAGAACGGGCAACCGCCGT
CGGAATCCGTGAGGTATTTGGCAAGCCATTCAACTTCCAGCGCCTCTTTGAACAACTCCTCGAAGCGCTTTCCCACAACA
AAGGCCATCAGTCTACACGTCGAGCAGAGTGA

Upstream 100 bases:

>100_bases
AAATATGACAATATGTCATTTTTAATATTGACAAATACGATAAATAGTCACACATTGACACGGCACGCCACACCACCCCA
TACGACCACAAGGAATCCCC

Downstream 100 bases:

>100_bases
CCATCACCAGAAACCCATAACCACGACGATCGGCAGCCTAAAAATTCCTTGCAATCGATTAACGACGGTGCTATAGATTT
AAGTCCTAGCGCCGAAGTGG

Product: response regulator receiver domain-containing protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 143; Mature: 143

Protein sequence:

>143_residues
MNPTQGHRTATIRPTRILVVDDDRDVLFFMKLALETEGIDVTCAERGEDALGLLAERSYGVLLTDQNMPGMDGFELAQHA
RRLQPELIIFMGTGHLYPGIEERATAVGIREVFGKPFNFQRLFEQLLEALSHNKGHQSTRRAE

Sequences:

>Translated_143_residues
MNPTQGHRTATIRPTRILVVDDDRDVLFFMKLALETEGIDVTCAERGEDALGLLAERSYGVLLTDQNMPGMDGFELAQHA
RRLQPELIIFMGTGHLYPGIEERATAVGIREVFGKPFNFQRLFEQLLEALSHNKGHQSTRRAE
>Mature_143_residues
MNPTQGHRTATIRPTRILVVDDDRDVLFFMKLALETEGIDVTCAERGEDALGLLAERSYGVLLTDQNMPGMDGFELAQHA
RRLQPELIIFMGTGHLYPGIEERATAVGIREVFGKPFNFQRLFEQLLEALSHNKGHQSTRRAE

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=127, Percent_Identity=30.7086614173228, Blast_Score=67, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 16123; Mature: 16123

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPTQGHRTATIRPTRILVVDDDRDVLFFMKLALETEGIDVTCAERGEDALGLLAERSYG
CCCCCCCEEEEECCEEEEEEECCCCEEEHHHHHHCCCCCEEEECCCCCCHHHHHCCCCCE
VLLTDQNMPGMDGFELAQHARRLQPELIIFMGTGHLYPGIEERATAVGIREVFGKPFNFQ
EEEECCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHH
RLFEQLLEALSHNKGHQSTRRAE
HHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNPTQGHRTATIRPTRILVVDDDRDVLFFMKLALETEGIDVTCAERGEDALGLLAERSYG
CCCCCCCEEEEECCEEEEEEECCCCEEEHHHHHHCCCCCEEEECCCCCCHHHHHCCCCCE
VLLTDQNMPGMDGFELAQHARRLQPELIIFMGTGHLYPGIEERATAVGIREVFGKPFNFQ
EEEECCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHH
RLFEQLLEALSHNKGHQSTRRAE
HHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]