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The map label for this gene is ntrC [H]

Identifier: 78222273

GI number: 78222273

Start: 1171210

End: 1172736

Strand: Direct

Name: ntrC [H]

Synonym: Gmet_1055

Alternate gene names: 78222273

Gene position: 1171210-1172736 (Clockwise)

Preceding gene: 78222272

Following gene: 78222277

Centisome position: 29.3

GC content: 64.24

Gene sequence:

>1527_bases
ATGTCACAGATCAAGGTATTGATTATCGATGACGAGGCGGATGTCTGCACCTTTTTCCGCCGCCTGCTGACCAGAAAAGG
GTATGACGTCGTCACGGCGTCAAAGGAGCCTGAGGCGATGCGGGCCATCGAAGAGCACCGCTTCACCGTGGCCATGGTGG
ATCTGAAGCTGCCGGATACCGACGGCCTGACCCTCCTTCAGGCGATCAAGGCCCGGCAGCCCGCCTGCGAAGTCATCATC
ATGACCGGCTACAGCACCATCAAGACGGCAGTCGCGGCCATGCAGCTGGGGGCCTACGAGTACCTGGAAAAGCCCTTCGA
CGACATCGACGAAATCGAGGCCCTGATCGAGAAGGCCGCCAGCCACGGGTTCAACCTCCAGCAGGGGAGCGCCGCCGCCG
AGGAGTGGGCCGAGGTGGCCCGGGCCGTCGGTTTCCAGGTGGGGGCATCCTTGGGCATGAGGCGGCTCGTATCCGTGGCC
CACAAGATCGCCGGCAAGAATGTCACCGTGCTGATCCAGGGGAGCACCGGCACCGGGAAAGAGGTCCTGGCCCGCTTTAT
CCATTCCGCATCGGGACGGGCGGATCAGGCGTTCATCCCGGTCAACTGCGGCGCCCTGCCGGAGAACCTCCTGGAAAGCG
AGCTCTTCGGCCACGAGAAAGGGGCCTTCACCGGCGCGGCCCAGCCCCGTCGCGGCATCTTCGAACTGGCCAACCGCGGC
ACCCTGTTCCTCGATGAAATCGGCGATGCCAGCCCGCAAATCCAGGTGAAACTGCTGCGGGTGCTGGAAACCGGCGAATT
CATGCGGGTAGGGGGTGAAAAACCCATCAAGACCGACGTGAGGGTGATCGCCGCCACCAACGTGGACCTGGAGCAGGCCA
TCCGGGAGAAGACGTTCCGCGAGGACCTCTACTACCGGCTCAACGTGGTCCGGCTCGAAATCCCGCCGCTCCGCTCCCGT
TCCGAGGACATCCCCCAGCTGGCGGAGCACTTTGTCCGTCAGCTCAACGCAGCGCTGCGGATTTCACCCCCGGCGCTGCG
CCTTCTGCAGGGGTACGGATGGCCGGGCAATATCCGCCAGCTTGCCAACGTCATCCGGCGCGCCGTGGTCATGTGCAGCG
GTGACACCATCCTCCCCGAGCATCTCGAAAGCACCCTTCTGAACAGCGCAGCCTTGGCCGGCGACACCTTTTCCCCCGTG
TCCTCGCAGGCCGGGGAGGGGGCTGTCTCCCTCTCCCCGGAGCGGCTCTGGCGAACCGACGTCAGCGTAGAGGAGTTGCG
GAAGCTGGGTGCCGATGAGCTGAAGCGGATGCTCGATTCCCTGCGGGCACTGGAGGGGAGGCTGATCACAGCCATGGGAG
AAGGGGGCGGGGCTGCGCGGCGGTGTCTCAAGGATACCGAGGAGGAAACCATCAGGCAGGCACTGGAACAGTACCGGTGG
AACATCACCGAGACGGCGAAGGTCCTGGGCATCGGCAGGAACACCCTCTACCGCAAGATCAAGCAGTACGGGCTGGGGAG
TTCCTGA

Upstream 100 bases:

>100_bases
AATGGCACCATCGAGGTGGAGAGCCGCCTTGGGGAGGGGAGCACCTTTTCGCTGCTCCTCCCCATAGAGCATCAGTGAGA
AGATCACAGCCGGAGCGAAA

Downstream 100 bases:

>100_bases
GTGGTCGGCGGCGGGTGTGCGAAGTTGCGGGAAGAGGAGGCGGGCGGGTCCGGGCGCTACAGAAGCATCCGGATTTCGAT
GAACGGCGTCTCCGGCTTGC

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MSQIKVLIIDDEADVCTFFRRLLTRKGYDVVTASKEPEAMRAIEEHRFTVAMVDLKLPDTDGLTLLQAIKARQPACEVII
MTGYSTIKTAVAAMQLGAYEYLEKPFDDIDEIEALIEKAASHGFNLQQGSAAAEEWAEVARAVGFQVGASLGMRRLVSVA
HKIAGKNVTVLIQGSTGTGKEVLARFIHSASGRADQAFIPVNCGALPENLLESELFGHEKGAFTGAAQPRRGIFELANRG
TLFLDEIGDASPQIQVKLLRVLETGEFMRVGGEKPIKTDVRVIAATNVDLEQAIREKTFREDLYYRLNVVRLEIPPLRSR
SEDIPQLAEHFVRQLNAALRISPPALRLLQGYGWPGNIRQLANVIRRAVVMCSGDTILPEHLESTLLNSAALAGDTFSPV
SSQAGEGAVSLSPERLWRTDVSVEELRKLGADELKRMLDSLRALEGRLITAMGEGGGAARRCLKDTEEETIRQALEQYRW
NITETAKVLGIGRNTLYRKIKQYGLGSS

Sequences:

>Translated_508_residues
MSQIKVLIIDDEADVCTFFRRLLTRKGYDVVTASKEPEAMRAIEEHRFTVAMVDLKLPDTDGLTLLQAIKARQPACEVII
MTGYSTIKTAVAAMQLGAYEYLEKPFDDIDEIEALIEKAASHGFNLQQGSAAAEEWAEVARAVGFQVGASLGMRRLVSVA
HKIAGKNVTVLIQGSTGTGKEVLARFIHSASGRADQAFIPVNCGALPENLLESELFGHEKGAFTGAAQPRRGIFELANRG
TLFLDEIGDASPQIQVKLLRVLETGEFMRVGGEKPIKTDVRVIAATNVDLEQAIREKTFREDLYYRLNVVRLEIPPLRSR
SEDIPQLAEHFVRQLNAALRISPPALRLLQGYGWPGNIRQLANVIRRAVVMCSGDTILPEHLESTLLNSAALAGDTFSPV
SSQAGEGAVSLSPERLWRTDVSVEELRKLGADELKRMLDSLRALEGRLITAMGEGGGAARRCLKDTEEETIRQALEQYRW
NITETAKVLGIGRNTLYRKIKQYGLGSS
>Mature_507_residues
SQIKVLIIDDEADVCTFFRRLLTRKGYDVVTASKEPEAMRAIEEHRFTVAMVDLKLPDTDGLTLLQAIKARQPACEVIIM
TGYSTIKTAVAAMQLGAYEYLEKPFDDIDEIEALIEKAASHGFNLQQGSAAAEEWAEVARAVGFQVGASLGMRRLVSVAH
KIAGKNVTVLIQGSTGTGKEVLARFIHSASGRADQAFIPVNCGALPENLLESELFGHEKGAFTGAAQPRRGIFELANRGT
LFLDEIGDASPQIQVKLLRVLETGEFMRVGGEKPIKTDVRVIAATNVDLEQAIREKTFREDLYYRLNVVRLEIPPLRSRS
EDIPQLAEHFVRQLNAALRISPPALRLLQGYGWPGNIRQLANVIRRAVVMCSGDTILPEHLESTLLNSAALAGDTFSPVS
SQAGEGAVSLSPERLWRTDVSVEELRKLGADELKRMLDSLRALEGRLITAMGEGGGAARRCLKDTEEETIRQALEQYRWN
ITETAKVLGIGRNTLYRKIKQYGLGSS

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=512, Percent_Identity=38.28125, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1790437, Length=392, Percent_Identity=46.1734693877551, Blast_Score=332, Evalue=4e-92,
Organism=Escherichia coli, GI1790299, Length=505, Percent_Identity=38.6138613861386, Blast_Score=326, Evalue=2e-90,
Organism=Escherichia coli, GI1788905, Length=379, Percent_Identity=44.3271767810026, Blast_Score=294, Evalue=8e-81,
Organism=Escherichia coli, GI87082117, Length=258, Percent_Identity=50.7751937984496, Blast_Score=248, Evalue=5e-67,
Organism=Escherichia coli, GI1789087, Length=342, Percent_Identity=41.812865497076, Blast_Score=241, Evalue=8e-65,
Organism=Escherichia coli, GI1789233, Length=366, Percent_Identity=40.1639344262295, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI87082152, Length=243, Percent_Identity=48.559670781893, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1786524, Length=250, Percent_Identity=45.2, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI1787583, Length=307, Percent_Identity=38.7622149837134, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI87081872, Length=220, Percent_Identity=48.1818181818182, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1789828, Length=245, Percent_Identity=38.3673469387755, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI87081858, Length=237, Percent_Identity=35.0210970464135, Blast_Score=125, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 55884; Mature: 55753

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQIKVLIIDDEADVCTFFRRLLTRKGYDVVTASKEPEAMRAIEEHRFTVAMVDLKLPDT
CCCEEEEEEECCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCEEEEEEEEECCCC
DGLTLLQAIKARQPACEVIIMTGYSTIKTAVAAMQLGAYEYLEKPFDDIDEIEALIEKAA
CCHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
SHGFNLQQGSAAAEEWAEVARAVGFQVGASLGMRRLVSVAHKIAGKNVTVLIQGSTGTGK
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHH
EVLARFIHSASGRADQAFIPVNCGALPENLLESELFGHEKGAFTGAAQPRRGIFELANRG
HHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCC
TLFLDEIGDASPQIQVKLLRVLETGEFMRVGGEKPIKTDVRVIAATNVDLEQAIREKTFR
CEEEECCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHH
EDLYYRLNVVRLEIPPLRSRSEDIPQLAEHFVRQLNAALRISPPALRLLQGYGWPGNIRQ
HHHHEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEECCCHHHHHHHCCCCCCCHHH
LANVIRRAVVMCSGDTILPEHLESTLLNSAALAGDTFSPVSSQAGEGAVSLSPERLWRTD
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCEECCHHHHHHCC
VSVEELRKLGADELKRMLDSLRALEGRLITAMGEGGGAARRCLKDTEEETIRQALEQYRW
CCHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHCC
NITETAKVLGIGRNTLYRKIKQYGLGSS
CHHHHHHHHHCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SQIKVLIIDDEADVCTFFRRLLTRKGYDVVTASKEPEAMRAIEEHRFTVAMVDLKLPDT
CCEEEEEEECCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCEEEEEEEEECCCC
DGLTLLQAIKARQPACEVIIMTGYSTIKTAVAAMQLGAYEYLEKPFDDIDEIEALIEKAA
CCHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
SHGFNLQQGSAAAEEWAEVARAVGFQVGASLGMRRLVSVAHKIAGKNVTVLIQGSTGTGK
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHH
EVLARFIHSASGRADQAFIPVNCGALPENLLESELFGHEKGAFTGAAQPRRGIFELANRG
HHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCC
TLFLDEIGDASPQIQVKLLRVLETGEFMRVGGEKPIKTDVRVIAATNVDLEQAIREKTFR
CEEEECCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHH
EDLYYRLNVVRLEIPPLRSRSEDIPQLAEHFVRQLNAALRISPPALRLLQGYGWPGNIRQ
HHHHEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEECCCHHHHHHHCCCCCCCHHH
LANVIRRAVVMCSGDTILPEHLESTLLNSAALAGDTFSPVSSQAGEGAVSLSPERLWRTD
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCEECCHHHHHHCC
VSVEELRKLGADELKRMLDSLRALEGRLITAMGEGGGAARRCLKDTEEETIRQALEQYRW
CCHHHHHHCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHCC
NITETAKVLGIGRNTLYRKIKQYGLGSS
CHHHHHHHHHCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8095910 [H]