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The map label for this gene is ackA

Identifier: 78222252

GI number: 78222252

Start: 1149287

End: 1150552

Strand: Direct

Name: ackA

Synonym: Gmet_1034

Alternate gene names: 78222252

Gene position: 1149287-1150552 (Clockwise)

Preceding gene: 78222250

Following gene: 78222265

Centisome position: 28.75

GC content: 56.79

Gene sequence:

>1266_bases
ATGGTAATTCTCGCCCTCAACTGCGGCAGCTCCTCGGTGAAATACCAGTTGTTCGACTGGGAAAAGAAAGAGGTCGTCGC
CAAGGGTATGGTGGAGCGGGTCATTATCGGCGACTCGTTCATCATGCACGAGGTTCCGGGCCGTGAAACCTACCGGAAGG
AATATGAGTGCCCCGACCACAAGGTGGCCATCGATCTGATTATCAGGACCCTCGTTGATGGGGAGCACGGTGTCCTCAAG
GATATCAATGAGATTTCCGCGGTTGGCCACCGTGTTGTCCACGGTGGAGAAATGTTCACCCGCTCGGTTCTCATTGACGA
GAAGGTGCTTGATGCCGTCAAGGATGTTCAGCACCTGGCACCCCTCCATAACCCGCCGAACATCGCCGGTATCGAGGCGG
CCCAGGCTGTTCTCCCGTCAGTGCCGCACGTTGCCATTTTCGATACGGCTTTCCACCAGACAATGCCCGAGCATGCCTAT
CTCTACCCGCTTCCCTACGAGTGGTATGAGAAATATGGCGTCCGCCGCTACGGTTTCCACGGGACCTCTCACCTCTTCGT
GTCGAAGCGGGCCGCTGTCCTGCTCGGCAAAAAACCTTCGGATTGCAACATCATCACCATGCATATCGGCAACGGTGTTT
CCCACTGTGCCATCAGGGGAGGGGTGTCCATCGATACCAGCATGGGGCTCACGCCGCTCGAAGGGGCGGTTATGGGCACC
CGGTGCGGCGATATCGATCCGGCTATTCCGGCCTTCATGATGCAGAAGGAAAACCTTTCCGCCAAGGAAATCGACAGCAT
TCTCAACAAAAAGAGCGGGGTGCTGGGCGTGACCGGGCGCTTCACCGACCGCCGCGATGTGATCGAAAACGCCAATAATG
GCGATCATCGCTGCAAGGTTGCCCTTGAGATCGAAGCGTACCGTCTCAAGAAGTATATCGGCACCTACATGGCGGTGCTC
GGCCGTCTCGATGCGGTGGTTTTCACTGCCGGCGTCGGTGAGATGGGGTGGCCCATCAGGGAGAAGACCATTGAAGGGCT
CGAAGGTATCGGCATCAAACTGGACCGTGAGCGGAACAAAAGCGCCATGACCAGAAAACGGGAGACGCTCATTACCACCG
ATGACTCCCCCATCAAGGTCTTCGTCATTCCCACCGACGAGGAACTTGTCTTTACCGAGGACGTGGTTGCCATACTCGAT
GGCACCTACACCGACCACATGAACTTTGACTACTCGTTTGCGCGGCAGGACTTCGTTCGGTCCTAG

Upstream 100 bases:

>100_bases
TTTTTGGGGCTTGCGACTTGCGGTATTTGAAATTATCCTGATATTCCACGTTTTATTACTGTTTAACGTTTTTTATTTTT
ACTCAATTTAGGAGGATATG

Downstream 100 bases:

>100_bases
CAAACAGGTGAACTGACCCTGCCTGGAAAAAGGCCGCCGTGGGTTTCCATGGCGGCCTTTCTGTGTTATGCGGACATCAA
CGAAAAAACGGCCGCCGGGC

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MVILALNCGSSSVKYQLFDWEKKEVVAKGMVERVIIGDSFIMHEVPGRETYRKEYECPDHKVAIDLIIRTLVDGEHGVLK
DINEISAVGHRVVHGGEMFTRSVLIDEKVLDAVKDVQHLAPLHNPPNIAGIEAAQAVLPSVPHVAIFDTAFHQTMPEHAY
LYPLPYEWYEKYGVRRYGFHGTSHLFVSKRAAVLLGKKPSDCNIITMHIGNGVSHCAIRGGVSIDTSMGLTPLEGAVMGT
RCGDIDPAIPAFMMQKENLSAKEIDSILNKKSGVLGVTGRFTDRRDVIENANNGDHRCKVALEIEAYRLKKYIGTYMAVL
GRLDAVVFTAGVGEMGWPIREKTIEGLEGIGIKLDRERNKSAMTRKRETLITTDDSPIKVFVIPTDEELVFTEDVVAILD
GTYTDHMNFDYSFARQDFVRS

Sequences:

>Translated_421_residues
MVILALNCGSSSVKYQLFDWEKKEVVAKGMVERVIIGDSFIMHEVPGRETYRKEYECPDHKVAIDLIIRTLVDGEHGVLK
DINEISAVGHRVVHGGEMFTRSVLIDEKVLDAVKDVQHLAPLHNPPNIAGIEAAQAVLPSVPHVAIFDTAFHQTMPEHAY
LYPLPYEWYEKYGVRRYGFHGTSHLFVSKRAAVLLGKKPSDCNIITMHIGNGVSHCAIRGGVSIDTSMGLTPLEGAVMGT
RCGDIDPAIPAFMMQKENLSAKEIDSILNKKSGVLGVTGRFTDRRDVIENANNGDHRCKVALEIEAYRLKKYIGTYMAVL
GRLDAVVFTAGVGEMGWPIREKTIEGLEGIGIKLDRERNKSAMTRKRETLITTDDSPIKVFVIPTDEELVFTEDVVAILD
GTYTDHMNFDYSFARQDFVRS
>Mature_421_residues
MVILALNCGSSSVKYQLFDWEKKEVVAKGMVERVIIGDSFIMHEVPGRETYRKEYECPDHKVAIDLIIRTLVDGEHGVLK
DINEISAVGHRVVHGGEMFTRSVLIDEKVLDAVKDVQHLAPLHNPPNIAGIEAAQAVLPSVPHVAIFDTAFHQTMPEHAY
LYPLPYEWYEKYGVRRYGFHGTSHLFVSKRAAVLLGKKPSDCNIITMHIGNGVSHCAIRGGVSIDTSMGLTPLEGAVMGT
RCGDIDPAIPAFMMQKENLSAKEIDSILNKKSGVLGVTGRFTDRRDVIENANNGDHRCKVALEIEAYRLKKYIGTYMAVL
GRLDAVVFTAGVGEMGWPIREKTIEGLEGIGIKLDRERNKSAMTRKRETLITTDDSPIKVFVIPTDEELVFTEDVVAILD
GTYTDHMNFDYSFARQDFVRS

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=397, Percent_Identity=43.8287153652393, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI145693184, Length=398, Percent_Identity=39.4472361809045, Blast_Score=310, Evalue=1e-85,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA_GEOMG (Q39WV0)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383999.1
- ProteinModelPortal:   Q39WV0
- SMR:   Q39WV0
- STRING:   Q39WV0
- GeneID:   3738140
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1034
- NMPDR:   fig|269799.3.peg.1124
- eggNOG:   COG0282
- HOGENOM:   HBG285863
- OMA:   SSDFRDV
- PhylomeDB:   Q39WV0
- ProtClustDB:   PRK00180
- BioCyc:   GMET269799:GMET_1034-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 46891; Mature: 46891

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVILALNCGSSSVKYQLFDWEKKEVVAKGMVERVIIGDSFIMHEVPGRETYRKEYECPDH
CEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHEEECCCEEEECCCCCHHHHHCCCCCCC
KVAIDLIIRTLVDGEHGVLKDINEISAVGHRVVHGGEMFTRSVLIDEKVLDAVKDVQHLA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHH
PLHNPPNIAGIEAAQAVLPSVPHVAIFDTAFHQTMPEHAYLYPLPYEWYEKYGVRRYGFH
CCCCCCCCCHHHHHHHHCCCCCCEEEEHHHHHHCCCCCEEEECCCHHHHHHCCCEEECCC
GTSHLFVSKRAAVLLGKKPSDCNIITMHIGNGVSHCAIRGGVSIDTSMGLTPLEGAVMGT
CCCEEEEECCEEEEECCCCCCCCEEEEECCCCCCEEEEECCCEECCCCCCCCCCCCEEEC
RCGDIDPAIPAFMMQKENLSAKEIDSILNKKSGVLGVTGRFTDRRDVIENANNGDHRCKV
CCCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCCCCCCEEEE
ALEIEAYRLKKYIGTYMAVLGRLDAVVFTAGVGEMGWPIREKTIEGLEGIGIKLDRERNK
EEEEHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHCCCCCCCEEEECCCCH
SAMTRKRETLITTDDSPIKVFVIPTDEELVFTEDVVAILDGTYTDHMNFDYSFARQDFVR
HHHHHHCCEEEECCCCCEEEEEEECCCCEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHC
S
C
>Mature Secondary Structure
MVILALNCGSSSVKYQLFDWEKKEVVAKGMVERVIIGDSFIMHEVPGRETYRKEYECPDH
CEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHEEECCCEEEECCCCCHHHHHCCCCCCC
KVAIDLIIRTLVDGEHGVLKDINEISAVGHRVVHGGEMFTRSVLIDEKVLDAVKDVQHLA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHH
PLHNPPNIAGIEAAQAVLPSVPHVAIFDTAFHQTMPEHAYLYPLPYEWYEKYGVRRYGFH
CCCCCCCCCHHHHHHHHCCCCCCEEEEHHHHHHCCCCCEEEECCCHHHHHHCCCEEECCC
GTSHLFVSKRAAVLLGKKPSDCNIITMHIGNGVSHCAIRGGVSIDTSMGLTPLEGAVMGT
CCCEEEEECCEEEEECCCCCCCCEEEEECCCCCCEEEEECCCEECCCCCCCCCCCCEEEC
RCGDIDPAIPAFMMQKENLSAKEIDSILNKKSGVLGVTGRFTDRRDVIENANNGDHRCKV
CCCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCCCCCCEEEE
ALEIEAYRLKKYIGTYMAVLGRLDAVVFTAGVGEMGWPIREKTIEGLEGIGIKLDRERNK
EEEEHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHCCCCCCCEEEECCCCH
SAMTRKRETLITTDDSPIKVFVIPTDEELVFTEDVVAILDGTYTDHMNFDYSFARQDFVR
HHHHHHCCEEEECCCCCEEEEEEECCCCEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA