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The map label for this gene is gcvH [H]

Identifier: 78222205

GI number: 78222205

Start: 1093191

End: 1093589

Strand: Direct

Name: gcvH [H]

Synonym: Gmet_0985

Alternate gene names: 78222205

Gene position: 1093191-1093589 (Clockwise)

Preceding gene: 78222204

Following gene: 78222206

Centisome position: 27.35

GC content: 60.65

Gene sequence:

>399_bases
ATGGATTTTCCTGATGAACTGAAGTACAGCAAGGAACATGTATGGGTCAGGGTCGAGGGTGACCGTGCCGTGATCGGCAT
TACCGATTATGCCCAGGAGAGCCTCGGCACCATCAGTGCCGTGGAGCTCCCGGCCGTCGGCGACGAGCTTGAGCAGGACG
ACTCCTTCGGCTCGGTGGAGGCCCGCAAGTGCCTGGCGGAGCTCTATGCGCCGGTCAGCGGCACCGTGGCCGAGGTTAAT
GACGAGCTGGAGTCGGCCCCGGAAATCCTGAACGACGACCCCTACGATGGCGGCTGGATCGCGGTGGTGGAACTTGCCGA
CCCCGAGGAGTTGAAATCCCTTCTGTCGGCAGAGGACTATGCCGAATCCGTAAGCCAGGAAGACGAAGAAGACGAATAG

Upstream 100 bases:

>100_bases
GACGACGATCCCGTTCCACAAGCGGATCATGGCCAACAAGGACTTCATGGAAGGGAACGTGGATACCGCCTTCCTTGAGA
AGATCGTGCTGGAGTAAGGT

Downstream 100 bases:

>100_bases
CCACAATCAGGTTCAGATGCAGGCGAAGGCGAGATTACCTATCTCGCCTTTGTTTTTGAGCAATTCATTTCATGATGCAG
GAGCGGTGTCACCATGTCAC

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 132; Mature: 132

Protein sequence:

>132_residues
MDFPDELKYSKEHVWVRVEGDRAVIGITDYAQESLGTISAVELPAVGDELEQDDSFGSVEARKCLAELYAPVSGTVAEVN
DELESAPEILNDDPYDGGWIAVVELADPEELKSLLSAEDYAESVSQEDEEDE

Sequences:

>Translated_132_residues
MDFPDELKYSKEHVWVRVEGDRAVIGITDYAQESLGTISAVELPAVGDELEQDDSFGSVEARKCLAELYAPVSGTVAEVN
DELESAPEILNDDPYDGGWIAVVELADPEELKSLLSAEDYAESVSQEDEEDE
>Mature_132_residues
MDFPDELKYSKEHVWVRVEGDRAVIGITDYAQESLGTISAVELPAVGDELEQDDSFGSVEARKCLAELYAPVSGTVAEVN
DELESAPEILNDDPYDGGWIAVVELADPEELKSLLSAEDYAESVSQEDEEDE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=122, Percent_Identity=37.7049180327869, Blast_Score=100, Evalue=4e-22,
Organism=Homo sapiens, GI89057342, Length=122, Percent_Identity=36.8852459016393, Blast_Score=97, Evalue=5e-21,
Organism=Escherichia coli, GI1789271, Length=120, Percent_Identity=46.6666666666667, Blast_Score=108, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17551294, Length=122, Percent_Identity=41.8032786885246, Blast_Score=105, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17507493, Length=122, Percent_Identity=40.9836065573771, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6319272, Length=129, Percent_Identity=39.5348837209302, Blast_Score=105, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17865652, Length=124, Percent_Identity=41.1290322580645, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 14470; Mature: 14470

Theoretical pI: Translated: 3.49; Mature: 3.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFPDELKYSKEHVWVRVEGDRAVIGITDYAQESLGTISAVELPAVGDELEQDDSFGSVE
CCCCHHHCCCCCEEEEEECCCEEEEEEHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCHH
ARKCLAELYAPVSGTVAEVNDELESAPEILNDDPYDGGWIAVVELADPEELKSLLSAEDY
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHH
AESVSQEDEEDE
HHHHHCCCCCCC
>Mature Secondary Structure
MDFPDELKYSKEHVWVRVEGDRAVIGITDYAQESLGTISAVELPAVGDELEQDDSFGSVE
CCCCHHHCCCCCEEEEEECCCEEEEEEHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCHH
ARKCLAELYAPVSGTVAEVNDELESAPEILNDDPYDGGWIAVVELADPEELKSLLSAEDY
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHH
AESVSQEDEEDE
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]