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The map label for this gene is pycA [H]

Identifier: 78222204

GI number: 78222204

Start: 1091847

End: 1093187

Strand: Direct

Name: pycA [H]

Synonym: Gmet_0984

Alternate gene names: 78222204

Gene position: 1091847-1093187 (Clockwise)

Preceding gene: 78222203

Following gene: 78222205

Centisome position: 27.31

GC content: 60.18

Gene sequence:

>1341_bases
ATGTTCCATAAAGTTCTGATTGCAAACCGGGGGGAGATTGCCCTGCGGGTCATCCGGGCCTGCAAGGAGTTGGGGATCAA
GACGGTGGCCGTCTATTCCGTCGCCGACAAGGATTCCCTTCACGTCAAGCTGGCCGACGAAAGCGTCTGCATCGGACCGG
CGCCAAGCCTCCAGAGCTACCTCAATATCAACGCCATCATCAGCGCCGCCGAAGTGACCGACGCCGAAGCAATCCATCCC
GGCTACGGTTTTCTCTCTGAAAATGCCGCCTTTGCCGAGATCTGCGAAAACTGCGGCATCACCTTCATCGGTCCCTCCTC
CGAGAGTATGCGGATCATGGGGGACAAGATCAGTGCCCGTCAGGCGGTGATCAAGGAGGATGTGCCGATCCTCCCCGGCA
CCAAGGAGGGGGTAAACGACGTCAACGAGGCCATAAAGATTGCCAAGGGGATCGGCTTCCCGGTCATCATCAAGGCGACG
GCCGGTGGTGGCGGACGGGGAATGAAGATCGTTCACTCTCCGGCAACCCTCCCCAACGCCTTTGCCACGGCACGGGCCGA
GGCCCAGGCCGGCTTCGGCAACCCCGAGGTCTACATCGAGAAGTACTGCGAAGAGCCCCGCCACGTGGAGATTCAGATCA
TGGCCGACAAGCACGGCAACGTGATCCACCTGGGCGAGCGGGACTGCTCCATCCAGCGGCGCCACCAGAAGATCATCGAG
GAGGCCCCGTGCCCGGTCATGACCCCGAACCTCCGCAAGGCCATGGGCGATGCAGCGGTCAGGGCCGCAAAGGCCGTTGG
CTATGACAGTGTCGGTACGGTCGAATTCCTGGTGGACAAGAACCTCAACTTCTACTTCATGGAGATGAACACCCGGGTCC
AGGTGGAGCATCCGGTCACCGAGATGATTACCGGCGTCGATATTGTCCGTGAGCAGATACGCTCCGCGGCGGGGCACAAA
CTCCGCTACAAGCAGGGCGATGTGAAGATCCAGGGGCACGCCATCGAGTGCCGCATCAACGCCGAGGACCCGGTGAAGTT
CACTCCGTGCCCCGGCAAGATCACCGGCTATCACACCCCCGGCGGCCTTGGGGTGCGGGTCGATTCCTTTGTGTACGACC
AGTATTCGGTGGTCCCCCACTATGATTCACTCATTTCCAAGCTCATCGTCCATGCCGAAACCCGCGAAGATGCCATCCGC
CGCATGGCCCGGGCACTGGACGAGTACATCATCGAAGGGATCAAGACGACGATCCCGTTCCACAAGCGGATCATGGCCAA
CAAGGACTTCATGGAAGGGAACGTGGATACCGCCTTCCTTGAGAAGATCGTGCTGGAGTAA

Upstream 100 bases:

>100_bases
CTGTTCATCGTCGAGCCGCTCTAACCCGAGACAACGGACGACACGTACGGGGACGGCAACAAGCAGTCGCCCTTTCAACC
CATGAGCGGAGATTTCGTAC

Downstream 100 bases:

>100_bases
GGTATGGATTTTCCTGATGAACTGAAGTACAGCAAGGAACATGTATGGGTCAGGGTCGAGGGTGACCGTGCCGTGATCGG
CATTACCGATTATGCCCAGG

Product: biotin carboxylase/acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha

Products: NA

Alternate protein names: Pyruvic carboxylase A [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MFHKVLIANRGEIALRVIRACKELGIKTVAVYSVADKDSLHVKLADESVCIGPAPSLQSYLNINAIISAAEVTDAEAIHP
GYGFLSENAAFAEICENCGITFIGPSSESMRIMGDKISARQAVIKEDVPILPGTKEGVNDVNEAIKIAKGIGFPVIIKAT
AGGGGRGMKIVHSPATLPNAFATARAEAQAGFGNPEVYIEKYCEEPRHVEIQIMADKHGNVIHLGERDCSIQRRHQKIIE
EAPCPVMTPNLRKAMGDAAVRAAKAVGYDSVGTVEFLVDKNLNFYFMEMNTRVQVEHPVTEMITGVDIVREQIRSAAGHK
LRYKQGDVKIQGHAIECRINAEDPVKFTPCPGKITGYHTPGGLGVRVDSFVYDQYSVVPHYDSLISKLIVHAETREDAIR
RMARALDEYIIEGIKTTIPFHKRIMANKDFMEGNVDTAFLEKIVLE

Sequences:

>Translated_446_residues
MFHKVLIANRGEIALRVIRACKELGIKTVAVYSVADKDSLHVKLADESVCIGPAPSLQSYLNINAIISAAEVTDAEAIHP
GYGFLSENAAFAEICENCGITFIGPSSESMRIMGDKISARQAVIKEDVPILPGTKEGVNDVNEAIKIAKGIGFPVIIKAT
AGGGGRGMKIVHSPATLPNAFATARAEAQAGFGNPEVYIEKYCEEPRHVEIQIMADKHGNVIHLGERDCSIQRRHQKIIE
EAPCPVMTPNLRKAMGDAAVRAAKAVGYDSVGTVEFLVDKNLNFYFMEMNTRVQVEHPVTEMITGVDIVREQIRSAAGHK
LRYKQGDVKIQGHAIECRINAEDPVKFTPCPGKITGYHTPGGLGVRVDSFVYDQYSVVPHYDSLISKLIVHAETREDAIR
RMARALDEYIIEGIKTTIPFHKRIMANKDFMEGNVDTAFLEKIVLE
>Mature_446_residues
MFHKVLIANRGEIALRVIRACKELGIKTVAVYSVADKDSLHVKLADESVCIGPAPSLQSYLNINAIISAAEVTDAEAIHP
GYGFLSENAAFAEICENCGITFIGPSSESMRIMGDKISARQAVIKEDVPILPGTKEGVNDVNEAIKIAKGIGFPVIIKAT
AGGGGRGMKIVHSPATLPNAFATARAEAQAGFGNPEVYIEKYCEEPRHVEIQIMADKHGNVIHLGERDCSIQRRHQKIIE
EAPCPVMTPNLRKAMGDAAVRAAKAVGYDSVGTVEFLVDKNLNFYFMEMNTRVQVEHPVTEMITGVDIVREQIRSAAGHK
LRYKQGDVKIQGHAIECRINAEDPVKFTPCPGKITGYHTPGGLGVRVDSFVYDQYSVVPHYDSLISKLIVHAETREDAIR
RMARALDEYIIEGIKTTIPFHKRIMANKDFMEGNVDTAFLEKIVLE

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotin carboxylation domain [H]

Homologues:

Organism=Homo sapiens, GI189095269, Length=445, Percent_Identity=50.561797752809, Blast_Score=457, Evalue=1e-129,
Organism=Homo sapiens, GI65506442, Length=445, Percent_Identity=50.561797752809, Blast_Score=457, Evalue=1e-129,
Organism=Homo sapiens, GI295821183, Length=445, Percent_Identity=50.561797752809, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI116805327, Length=440, Percent_Identity=50, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI106049528, Length=446, Percent_Identity=47.9820627802691, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI106049295, Length=446, Percent_Identity=47.9820627802691, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI106049292, Length=446, Percent_Identity=47.9820627802691, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI38679971, Length=511, Percent_Identity=32.4853228962818, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI38679960, Length=511, Percent_Identity=32.4853228962818, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI38679977, Length=511, Percent_Identity=32.4853228962818, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI38679967, Length=511, Percent_Identity=32.4853228962818, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI38679974, Length=511, Percent_Identity=32.4853228962818, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI134142062, Length=512, Percent_Identity=32.03125, Blast_Score=254, Evalue=1e-67,
Organism=Escherichia coli, GI1789654, Length=443, Percent_Identity=59.5936794582393, Blast_Score=516, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI17567343, Length=448, Percent_Identity=47.9910714285714, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71987519, Length=441, Percent_Identity=48.9795918367347, Blast_Score=406, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17562816, Length=447, Percent_Identity=46.9798657718121, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI71997168, Length=506, Percent_Identity=29.4466403162055, Blast_Score=201, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI71997163, Length=506, Percent_Identity=29.4466403162055, Blast_Score=201, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI133931226, Length=485, Percent_Identity=30.3092783505155, Blast_Score=194, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6319685, Length=448, Percent_Identity=44.6428571428571, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6319695, Length=447, Percent_Identity=44.5190156599553, Blast_Score=365, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6321376, Length=447, Percent_Identity=45.8612975391499, Blast_Score=359, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6323863, Length=481, Percent_Identity=34.0956340956341, Blast_Score=243, Evalue=4e-65,
Organism=Saccharomyces cerevisiae, GI6324343, Length=516, Percent_Identity=31.5891472868217, Blast_Score=233, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24651757, Length=443, Percent_Identity=48.5327313769752, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI281363050, Length=444, Percent_Identity=48.8738738738739, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24652224, Length=444, Percent_Identity=48.8738738738739, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24652222, Length=444, Percent_Identity=48.8738738738739, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24652220, Length=444, Percent_Identity=48.8738738738739, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24652218, Length=444, Percent_Identity=48.8738738738739, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24652212, Length=444, Percent_Identity=48.8738738738739, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24652210, Length=444, Percent_Identity=48.8738738738739, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24652214, Length=444, Percent_Identity=48.8738738738739, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI19921944, Length=444, Percent_Identity=48.8738738738739, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24652216, Length=444, Percent_Identity=48.8738738738739, Blast_Score=410, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24651759, Length=407, Percent_Identity=47.4201474201474, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI161076409, Length=509, Percent_Identity=31.434184675835, Blast_Score=248, Evalue=6e-66,
Organism=Drosophila melanogaster, GI24586458, Length=509, Percent_Identity=31.434184675835, Blast_Score=248, Evalue=6e-66,
Organism=Drosophila melanogaster, GI161076407, Length=509, Percent_Identity=31.434184675835, Blast_Score=248, Evalue=7e-66,
Organism=Drosophila melanogaster, GI24586460, Length=509, Percent_Identity=31.434184675835, Blast_Score=248, Evalue=7e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004549
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2 [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 48893; Mature: 48893

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50975 ATP_GRASP ; PS00866 CPSASE_1 ; PS00867 CPSASE_2 ; PS50979 BC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHKVLIANRGEIALRVIRACKELGIKTVAVYSVADKDSLHVKLADESVCIGPAPSLQSY
CCCEEEEECCCHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEECCCCEEECCCCCHHHH
LNINAIISAAEVTDAEAIHPGYGFLSENAAFAEICENCGITFIGPSSESMRIMGDKISAR
HCHHHEEEEHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCEEEECCCCCCEEEECCHHHHH
QAVIKEDVPILPGTKEGVNDVNEAIKIAKGIGFPVIIKATAGGGGRGMKIVHSPATLPNA
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEECCCCCCCH
FATARAEAQAGFGNPEVYIEKYCEEPRHVEIQIMADKHGNVIHLGERDCSIQRRHQKIIE
HHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCEEEECCCCCHHHHHHHHHHH
EAPCPVMTPNLRKAMGDAAVRAAKAVGYDSVGTVEFLVDKNLNFYFMEMNTRVQVEHPVT
HCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCEEEECCCHH
EMITGVDIVREQIRSAAGHKLRYKQGDVKIQGHAIECRINAEDPVKFTPCPGKITGYHTP
HHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEEEEEEECCCCCEEECCCCCCCEEEECC
GGLGVRVDSFVYDQYSVVPHYDSLISKLIVHAETREDAIRRMARALDEYIIEGIKTTIPF
CCCCEEEEEEEECCEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
HKRIMANKDFMEGNVDTAFLEKIVLE
HHHHHCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MFHKVLIANRGEIALRVIRACKELGIKTVAVYSVADKDSLHVKLADESVCIGPAPSLQSY
CCCEEEEECCCHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEECCCCEEECCCCCHHHH
LNINAIISAAEVTDAEAIHPGYGFLSENAAFAEICENCGITFIGPSSESMRIMGDKISAR
HCHHHEEEEHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCEEEECCCCCCEEEECCHHHHH
QAVIKEDVPILPGTKEGVNDVNEAIKIAKGIGFPVIIKATAGGGGRGMKIVHSPATLPNA
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEECCCCCCCH
FATARAEAQAGFGNPEVYIEKYCEEPRHVEIQIMADKHGNVIHLGERDCSIQRRHQKIIE
HHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCEEEECCCCCHHHHHHHHHHH
EAPCPVMTPNLRKAMGDAAVRAAKAVGYDSVGTVEFLVDKNLNFYFMEMNTRVQVEHPVT
HCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCEEEECCCHH
EMITGVDIVREQIRSAAGHKLRYKQGDVKIQGHAIECRINAEDPVKFTPCPGKITGYHTP
HHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEEEEEEECCCCCEEECCCCCCCEEEECC
GGLGVRVDSFVYDQYSVVPHYDSLISKLIVHAETREDAIRRMARALDEYIIEGIKTTIPF
CCCCEEEEEEEECCEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
HKRIMANKDFMEGNVDTAFLEKIVLE
HHHHHCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463; 9478969 [H]