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The map label for this gene is pfkA [H]

Identifier: 78222158

GI number: 78222158

Start: 1039789

End: 1040748

Strand: Reverse

Name: pfkA [H]

Synonym: Gmet_0938

Alternate gene names: 78222158

Gene position: 1040748-1039789 (Counterclockwise)

Preceding gene: 78222159

Following gene: 78222137

Centisome position: 26.04

GC content: 64.58

Gene sequence:

>960_bases
ATGAAAAAGATCGGCATTCTCACGAGCGGCGGCGACTGCTCCGGCATGAACGCCGCCATCCGGGCGGCGGTACGGACCGC
GATCCGCCTGAACATCGAGGTGGTGGGATTCAGAAAGGGATATCTGGGGCTCATGAAGGGGGATGCCATCCCCCTCGACA
CCAAGGCGGTTTCGGGCATCCTCCACCGGGGTGGGACGTTCCTCCAGTCGGCCCGCTCGCCGGAATTCAAGACCCCCGAG
GGGCAGCAGAAGGCCATCGACAACCTGAAGGAGTTGGGCGTCGAAGGACTCGTGGTGCTCGGTGGCGACGGCTCCCTAAC
CGGCGCCCTGGCGCTCCACCGGCTCGGCCTGCCGGTGGTCGGCATCCCGTCCAGCATTGACAACGACATCCCCTACACCG
ACATGGCCCTCGGTGTTGATACGGCCCTCAACAACATCATCTACGCCGTCGACTGCATCAAGGATACGGCCAGCTCCCAC
GCCCGGGCCTTTGTGATCGAGGTCATGGGGCGCAACTCGGGGTACCTGGCCAGCATCTCCGCCATCGCCACCGGTGCCGA
GTTCGCCCTGGTCCCCGAACGGGAATACGACCTGGCCGAGATCTGCCAACAGCTCCGAGCACGCTTCGAGGAGGGGCGCG
ATAACGCCATCATCATCCTCGCCGAAGGCGCCGGCCATGCCCGGGAGATCTCCGACAGCATCAAGGACGCCATCGGCTTC
GAGACGCGGATCACGGTGCTGGGCCACTACCAGCGCGGCGGCGCGCCGACCGTCTTCGATCGGCTCCTGGCAAGCCGTTT
CGGCAAGCGGGCGGTGGAACTCCTCATCTCCGGCAACTCCGGGGTGATGGTGGGACTTTCCTGCAATGCCATTCTCGCCA
CGCCCCTGGAAGACGTCATCAAAGGCGAGAAACGCCCCCAGGACGAGGTATTGCGGCTGGCAGAGGTGTTAGGTGTTTAG

Upstream 100 bases:

>100_bases
TCCGGGGGAAGACCTTTTACGCCCCCCGCGGGCATGGCTACGAAAAGACGATCAAGGAACGGATGGAATATCTGCGGACT
TTACAGGAGAGGAACAAGCA

Downstream 100 bases:

>100_bases
TGTTAGGGAACCATGTGGGAGCGGTTCAGGATCGCACAAGGGCGACCAGATCGCCATGGTGGTCGATGCACGTCATGGCG
GGGAGGTCTGCCTTGTAGGC

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKKIGILTSGGDCSGMNAAIRAAVRTAIRLNIEVVGFRKGYLGLMKGDAIPLDTKAVSGILHRGGTFLQSARSPEFKTPE
GQQKAIDNLKELGVEGLVVLGGDGSLTGALALHRLGLPVVGIPSSIDNDIPYTDMALGVDTALNNIIYAVDCIKDTASSH
ARAFVIEVMGRNSGYLASISAIATGAEFALVPEREYDLAEICQQLRARFEEGRDNAIIILAEGAGHAREISDSIKDAIGF
ETRITVLGHYQRGGAPTVFDRLLASRFGKRAVELLISGNSGVMVGLSCNAILATPLEDVIKGEKRPQDEVLRLAEVLGV

Sequences:

>Translated_319_residues
MKKIGILTSGGDCSGMNAAIRAAVRTAIRLNIEVVGFRKGYLGLMKGDAIPLDTKAVSGILHRGGTFLQSARSPEFKTPE
GQQKAIDNLKELGVEGLVVLGGDGSLTGALALHRLGLPVVGIPSSIDNDIPYTDMALGVDTALNNIIYAVDCIKDTASSH
ARAFVIEVMGRNSGYLASISAIATGAEFALVPEREYDLAEICQQLRARFEEGRDNAIIILAEGAGHAREISDSIKDAIGF
ETRITVLGHYQRGGAPTVFDRLLASRFGKRAVELLISGNSGVMVGLSCNAILATPLEDVIKGEKRPQDEVLRLAEVLGV
>Mature_319_residues
MKKIGILTSGGDCSGMNAAIRAAVRTAIRLNIEVVGFRKGYLGLMKGDAIPLDTKAVSGILHRGGTFLQSARSPEFKTPE
GQQKAIDNLKELGVEGLVVLGGDGSLTGALALHRLGLPVVGIPSSIDNDIPYTDMALGVDTALNNIIYAVDCIKDTASSH
ARAFVIEVMGRNSGYLASISAIATGAEFALVPEREYDLAEICQQLRARFEEGRDNAIIILAEGAGHAREISDSIKDAIGF
ETRITVLGHYQRGGAPTVFDRLLASRFGKRAVELLISGNSGVMVGLSCNAILATPLEDVIKGEKRPQDEVLRLAEVLGV

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=356, Percent_Identity=42.4157303370786, Blast_Score=222, Evalue=3e-58,
Organism=Homo sapiens, GI266453768, Length=358, Percent_Identity=41.340782122905, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI266453748, Length=358, Percent_Identity=41.340782122905, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI4505749, Length=358, Percent_Identity=41.340782122905, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI266453619, Length=358, Percent_Identity=41.340782122905, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI48762920, Length=344, Percent_Identity=40.9883720930233, Blast_Score=213, Evalue=1e-55,
Organism=Escherichia coli, GI1790350, Length=306, Percent_Identity=46.4052287581699, Blast_Score=275, Evalue=4e-75,
Organism=Caenorhabditis elegans, GI25147584, Length=333, Percent_Identity=40.2402402402402, Blast_Score=214, Evalue=7e-56,
Organism=Caenorhabditis elegans, GI17558788, Length=308, Percent_Identity=38.3116883116883, Blast_Score=195, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI72003601, Length=330, Percent_Identity=29.6969696969697, Blast_Score=146, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6321679, Length=341, Percent_Identity=40.1759530791789, Blast_Score=216, Evalue=5e-57,
Organism=Saccharomyces cerevisiae, GI6323861, Length=343, Percent_Identity=35.8600583090379, Blast_Score=187, Evalue=1e-48,
Organism=Drosophila melanogaster, GI28573326, Length=356, Percent_Identity=41.0112359550562, Blast_Score=228, Evalue=3e-60,
Organism=Drosophila melanogaster, GI24652337, Length=356, Percent_Identity=40.1685393258427, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI17647809, Length=356, Percent_Identity=40.1685393258427, Blast_Score=222, Evalue=3e-58,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 33781; Mature: 33781

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIGILTSGGDCSGMNAAIRAAVRTAIRLNIEVVGFRKGYLGLMKGDAIPLDTKAVSGI
CCCEEEEECCCCCCCHHHHHHHHHHHHHEEEEEEEEECCCCEEEECCCCCCCCHHHHHHH
LHRGGTFLQSARSPEFKTPEGQQKAIDNLKELGVEGLVVLGGDGSLTGALALHRLGLPVV
HHCCCHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCEE
GIPSSIDNDIPYTDMALGVDTALNNIIYAVDCIKDTASSHARAFVIEVMGRNSGYLASIS
ECCCCCCCCCCCHHHHHHHHHHHHHHEEEEHHHHHHHCCCCEEEEEEEECCCCCCEEEHH
AIATGAEFALVPEREYDLAEICQQLRARFEEGRDNAIIILAEGAGHAREISDSIKDAIGF
HHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCC
ETRITVLGHYQRGGAPTVFDRLLASRFGKRAVELLISGNSGVMVGLSCNAILATPLEDVI
CEEEEEEEECCCCCCHHHHHHHHHHHHCHHEEEEEEECCCCEEEEEECCEEEECCHHHHH
KGEKRPQDEVLRLAEVLGV
CCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKKIGILTSGGDCSGMNAAIRAAVRTAIRLNIEVVGFRKGYLGLMKGDAIPLDTKAVSGI
CCCEEEEECCCCCCCHHHHHHHHHHHHHEEEEEEEEECCCCEEEECCCCCCCCHHHHHHH
LHRGGTFLQSARSPEFKTPEGQQKAIDNLKELGVEGLVVLGGDGSLTGALALHRLGLPVV
HHCCCHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCEE
GIPSSIDNDIPYTDMALGVDTALNNIIYAVDCIKDTASSHARAFVIEVMGRNSGYLASIS
ECCCCCCCCCCCHHHHHHHHHHHHHHEEEEHHHHHHHCCCCEEEEEEEECCCCCCEEEHH
AIATGAEFALVPEREYDLAEICQQLRARFEEGRDNAIIILAEGAGHAREISDSIKDAIGF
HHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCC
ETRITVLGHYQRGGAPTVFDRLLASRFGKRAVELLISGNSGVMVGLSCNAILATPLEDVI
CEEEEEEEECCCCCCHHHHHHHHHHHHCHHEEEEEEECCCCEEEEEECCEEEECCHHHHH
KGEKRPQDEVLRLAEVLGV
CCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA