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The map label for this gene is purC

Identifier: 78222136

GI number: 78222136

Start: 1010003

End: 1010893

Strand: Reverse

Name: purC

Synonym: Gmet_0916

Alternate gene names: 78222136

Gene position: 1010893-1010003 (Counterclockwise)

Preceding gene: 78222137

Following gene: 78222120

Centisome position: 25.29

GC content: 61.5

Gene sequence:

>891_bases
ATGGCAAACCTGGTCCTCAAAACCGACTTCCCCGATCTGAAGCTTGTGGCCCGCGGCAAGGTCCGCGACATCTATGACCT
GGGCGAAGCATTGCTGATCGTTACCACCGACCGGATTTCCGCCTTCGACGTCATCATGAACGAGGGGATCCCGGACAAGG
GGTACGTCCTCACCCAGATCTCCGCCTTCTGGTTCCGCCAGATGGAGGACATCATCCCGAACCACATCATCTCCACCGAG
GTAAAGGACTTCCCGGCCGAGTGCCAGAAGTACGCCGCCGACCTGGAAGGGCGCTCCATGCTGGTCAAGAAGGCGAACCC
CCTCCCGGCGGAGTGCATCGTGCGGGGCTACATCTCCGGCTCCGGGTGGAAGGACTACAAGGCCACCGGTTCCATCTGCG
GCATCAAGCTCCCGGCAGGGCTCGTGGAATCCGACAAGCTGGAGGAGCCGATCTTCACCCCCTCCACCAAGGCCGAGCTC
GGCACCCACGACGAGAACATCTCCTTCGACAGGATGGTCGAAATGATGGGGAAGGAACTGGCCGGGAAGGTGCGCGACGT
CACCATCGCCATCTACAAGCGGGCCCGGGACATCGCCGACGCCAAGGGGATCATCATTGCCGACACCAAGTTCGAATACG
GCATTTACAACGGCGAACTGATCATCATCGACGAGTGCATGACCCCCGACTCATCCCGCTTCTGGCCCAAGGACAGCTAC
AAGCCGGGCGGGGCGCAGCCCTCCTTCGACAAGCAGTTCCTGCGGGACTACCTGGAGACCCTCGACTGGAACAAGACCGC
TCCGGCCCCGCCGCTCCCCGCCGAGATCGTCAAGAAGACTGGCGAAAAATACATGGAGGCCCTGGTGCGGCTCACGGGGA
AAGGGAAGTAA

Upstream 100 bases:

>100_bases
CTCAAGGAGCTGCGCGCGAAGGCGAAGATCGAGATCAACCCTGCCCTCAAGGATCAGTAGCGGGCACTATCACACGATTT
ACGAAAAAGGAGACTGAGCC

Downstream 100 bases:

>100_bases
TCGCCAACTCCGATTCAAACCGGTACGCTCCCGACACACGAAAGCCCCGACCTTTTCAGGCCGGGGCTTTTTTCATTTCC
TCCGCGATTCGCCGTCGTTC

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MANLVLKTDFPDLKLVARGKVRDIYDLGEALLIVTTDRISAFDVIMNEGIPDKGYVLTQISAFWFRQMEDIIPNHIISTE
VKDFPAECQKYAADLEGRSMLVKKANPLPAECIVRGYISGSGWKDYKATGSICGIKLPAGLVESDKLEEPIFTPSTKAEL
GTHDENISFDRMVEMMGKELAGKVRDVTIAIYKRARDIADAKGIIIADTKFEYGIYNGELIIIDECMTPDSSRFWPKDSY
KPGGAQPSFDKQFLRDYLETLDWNKTAPAPPLPAEIVKKTGEKYMEALVRLTGKGK

Sequences:

>Translated_296_residues
MANLVLKTDFPDLKLVARGKVRDIYDLGEALLIVTTDRISAFDVIMNEGIPDKGYVLTQISAFWFRQMEDIIPNHIISTE
VKDFPAECQKYAADLEGRSMLVKKANPLPAECIVRGYISGSGWKDYKATGSICGIKLPAGLVESDKLEEPIFTPSTKAEL
GTHDENISFDRMVEMMGKELAGKVRDVTIAIYKRARDIADAKGIIIADTKFEYGIYNGELIIIDECMTPDSSRFWPKDSY
KPGGAQPSFDKQFLRDYLETLDWNKTAPAPPLPAEIVKKTGEKYMEALVRLTGKGK
>Mature_295_residues
ANLVLKTDFPDLKLVARGKVRDIYDLGEALLIVTTDRISAFDVIMNEGIPDKGYVLTQISAFWFRQMEDIIPNHIISTEV
KDFPAECQKYAADLEGRSMLVKKANPLPAECIVRGYISGSGWKDYKATGSICGIKLPAGLVESDKLEEPIFTPSTKAELG
THDENISFDRMVEMMGKELAGKVRDVTIAIYKRARDIADAKGIIIADTKFEYGIYNGELIIIDECMTPDSSRFWPKDSYK
PGGAQPSFDKQFLRDYLETLDWNKTAPAPPLPAEIVKKTGEKYMEALVRLTGKGK

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family

Homologues:

Organism=Escherichia coli, GI1788820, Length=229, Percent_Identity=29.2576419213974, Blast_Score=69, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319326, Length=291, Percent_Identity=50.5154639175258, Blast_Score=276, Evalue=3e-75,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PUR7_GEOMG (Q39X66)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383883.1
- HSSP:   P27616
- ProteinModelPortal:   Q39X66
- SMR:   Q39X66
- STRING:   Q39X66
- GeneID:   3738254
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0916
- NMPDR:   fig|269799.3.peg.991
- eggNOG:   COG0152
- HOGENOM:   HBG555040
- OMA:   LTLMDEV
- PhylomeDB:   Q39X66
- ProtClustDB:   PRK13961
- BioCyc:   GMET269799:GMET_0916-MONOMER
- HAMAP:   MF_00137
- InterPro:   IPR013816
- InterPro:   IPR001636
- Gene3D:   G3DSA:3.30.470.20
- PANTHER:   PTHR11609
- TIGRFAMs:   TIGR00081

Pfam domain/function: PF01259 SAICAR_synt

EC number: =6.3.2.6

Molecular weight: Translated: 33042; Mature: 32911

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS01057 SAICAR_SYNTHETASE_1; PS01058 SAICAR_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANLVLKTDFPDLKLVARGKVRDIYDLGEALLIVTTDRISAFDVIMNEGIPDKGYVLTQI
CCCEEEECCCCCEEEEECCCHHHHHHCCCEEEEEEECCCHHHHHHHHCCCCCCCEEHHHH
SAFWFRQMEDIIPNHIISTEVKDFPAECQKYAADLEGRSMLVKKANPLPAECIVRGYISG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHEECCCCC
SGWKDYKATGSICGIKLPAGLVESDKLEEPIFTPSTKAELGTHDENISFDRMVEMMGKEL
CCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
AGKVRDVTIAIYKRARDIADAKGIIIADTKFEYGIYNGELIIIDECMTPDSSRFWPKDSY
CCHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEECCEEEEEECCCCCCCCCCCCCCCC
KPGGAQPSFDKQFLRDYLETLDWNKTAPAPPLPAEIVKKTGEKYMEALVRLTGKGK
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ANLVLKTDFPDLKLVARGKVRDIYDLGEALLIVTTDRISAFDVIMNEGIPDKGYVLTQI
CCEEEECCCCCEEEEECCCHHHHHHCCCEEEEEEECCCHHHHHHHHCCCCCCCEEHHHH
SAFWFRQMEDIIPNHIISTEVKDFPAECQKYAADLEGRSMLVKKANPLPAECIVRGYISG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHEECCCCC
SGWKDYKATGSICGIKLPAGLVESDKLEEPIFTPSTKAELGTHDENISFDRMVEMMGKEL
CCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
AGKVRDVTIAIYKRARDIADAKGIIIADTKFEYGIYNGELIIIDECMTPDSSRFWPKDSY
CCHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEECCEEEEEECCCCCCCCCCCCCCCC
KPGGAQPSFDKQFLRDYLETLDWNKTAPAPPLPAEIVKKTGEKYMEALVRLTGKGK
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA