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The map label for this gene is trmFO

Identifier: 78222113

GI number: 78222113

Start: 984835

End: 986139

Strand: Direct

Name: trmFO

Synonym: Gmet_0893

Alternate gene names: 78222113

Gene position: 984835-986139 (Clockwise)

Preceding gene: 78222112

Following gene: 78222114

Centisome position: 24.64

GC content: 64.98

Gene sequence:

>1305_bases
GTGACGGATGCTATTACCATCATCGGCGGAGGGCTCGCCGGATGCGAGGCTGTCTGGCAGGCGGCGGAGCGCGGGGTGCG
GGTCACGCTCCACGAGATGAAGCCCCATGCCTTTTCGCCGGCACACCACCTGGCGGGGCTCGCGGAACTCGTCTGCTCCA
ACTCGCTCCGGGGAGAGTCCCTGGAGAATGCCGTGGGGCTCCTCAAGGAGGAACTGCGGCGGGCCGGCTCCCTCATCATG
GCAGCGGCCGACGCCACCCGCGTGCCGGCCGGCGGAGCCCTGGCGGTGGACCGTGAGCTCTTCTCCGCCTTTGTCACCGA
GAGAATCGAGAACCATCCCCTTGTCGACCTCATGCGGGGAGAGGTGGCGGATATTCCTTCCGAGGATATCGTGATTGTGG
CTACGGGCCCCCTCACGAGCGACGGGCTGGCCGAAAGAATCAGGGCGATCACCGGGCCGAACCTCTACTTCTACGATGCC
ATCGCGCCCATCGTGACCGCCGAATCCCTCGATATGACCAAGGTGTTCCGGGCCTCTCGCTACGGCAAGGGGGACGGCGA
CGACTACCTGAACTGTCCCTTGAACCAGGAAGAGTATGAGGGGTTCGTGGACGCGATCCTTGCCGCCGAGAAAGTGCCTG
CCAGGGATTTTGAAAAAGTGGTACACTTCGAAGGGTGCATGCCGGTTGAAGAAATGGCCGAGCGGGGTCGTGAGACCCTC
CGCTTCGGCCCCCTGAAGCCCGTGGGGCTCGCGGATCCCCGCACCGGGGAGGAGCCCCATGCCGTGGTGCAGCTCAGGGC
CGAGAACCGTGAAGGGACCATGTTCAACCTGGTGGGCTTCCAGACCAAGCTCACCTATCCGGAGCAGCGGAGGATCTTCA
GGATGATCCCCGGCCTGGAGAACGCCGAGTTCGTCCGGCTCGGTTCCATGCACCGCAACACCTTCATCAACGCGCCAGCC
CTCCTGGCCCCCACGTTCCAGCTGAAGGGGGATGCCCGTATTCTCTTTGCCGGCCAGATCACCGGGGTCGAGGGATATGT
GGAGTCGGCGGGGAGCGGTTTCCTGGCCGGCGTCAACGCTGCCCGGCTCGTCCGGGGAGAGGAGCCGGTGGTGCCGCCGC
TGGCCACTGCCCTCGGCGCCCTGGTGACCCACATCACCAACGCTGACGTGAAGCACTTCCAGCCCATGAACGTCAACTAC
GGGCTCTTCCCTCCCCTGGAGGGGAAGGTGAAGAAGAAGGAGCGGCGGGGGAAGCTTGCGGAGCGGGCACTGGCTGAGCT
GGACCGTTGGATAGGAACAGTGTAG

Upstream 100 bases:

>100_bases
GGTACCTCACCATCTGGGACGAGGAGCGGGCCAAGGCGCGGCTCGTGGCGGGGCATCCGCTGCAGCGCTACGAAATACCC
GACGAGGAGGGGAACGCCCA

Downstream 100 bases:

>100_bases
GGGCGCTGCTTGCCGCGCCCGCATCGAAAATGGGCGCGGCAAGCAGCGCCCCTGCGAGGAACCCATGGACGACAAGGAAA
AACAGGAAATATCCCTCATC

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MTDAITIIGGGLAGCEAVWQAAERGVRVTLHEMKPHAFSPAHHLAGLAELVCSNSLRGESLENAVGLLKEELRRAGSLIM
AAADATRVPAGGALAVDRELFSAFVTERIENHPLVDLMRGEVADIPSEDIVIVATGPLTSDGLAERIRAITGPNLYFYDA
IAPIVTAESLDMTKVFRASRYGKGDGDDYLNCPLNQEEYEGFVDAILAAEKVPARDFEKVVHFEGCMPVEEMAERGRETL
RFGPLKPVGLADPRTGEEPHAVVQLRAENREGTMFNLVGFQTKLTYPEQRRIFRMIPGLENAEFVRLGSMHRNTFINAPA
LLAPTFQLKGDARILFAGQITGVEGYVESAGSGFLAGVNAARLVRGEEPVVPPLATALGALVTHITNADVKHFQPMNVNY
GLFPPLEGKVKKKERRGKLAERALAELDRWIGTV

Sequences:

>Translated_434_residues
MTDAITIIGGGLAGCEAVWQAAERGVRVTLHEMKPHAFSPAHHLAGLAELVCSNSLRGESLENAVGLLKEELRRAGSLIM
AAADATRVPAGGALAVDRELFSAFVTERIENHPLVDLMRGEVADIPSEDIVIVATGPLTSDGLAERIRAITGPNLYFYDA
IAPIVTAESLDMTKVFRASRYGKGDGDDYLNCPLNQEEYEGFVDAILAAEKVPARDFEKVVHFEGCMPVEEMAERGRETL
RFGPLKPVGLADPRTGEEPHAVVQLRAENREGTMFNLVGFQTKLTYPEQRRIFRMIPGLENAEFVRLGSMHRNTFINAPA
LLAPTFQLKGDARILFAGQITGVEGYVESAGSGFLAGVNAARLVRGEEPVVPPLATALGALVTHITNADVKHFQPMNVNY
GLFPPLEGKVKKKERRGKLAERALAELDRWIGTV
>Mature_433_residues
TDAITIIGGGLAGCEAVWQAAERGVRVTLHEMKPHAFSPAHHLAGLAELVCSNSLRGESLENAVGLLKEELRRAGSLIMA
AADATRVPAGGALAVDRELFSAFVTERIENHPLVDLMRGEVADIPSEDIVIVATGPLTSDGLAERIRAITGPNLYFYDAI
APIVTAESLDMTKVFRASRYGKGDGDDYLNCPLNQEEYEGFVDAILAAEKVPARDFEKVVHFEGCMPVEEMAERGRETLR
FGPLKPVGLADPRTGEEPHAVVQLRAENREGTMFNLVGFQTKLTYPEQRRIFRMIPGLENAEFVRLGSMHRNTFINAPAL
LAPTFQLKGDARILFAGQITGVEGYVESAGSGFLAGVNAARLVRGEEPVVPPLATALGALVTHITNADVKHFQPMNVNYG
LFPPLEGKVKKKERRGKLAERALAELDRWIGTV

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

Organism=Escherichia coli, GI2367273, Length=114, Percent_Identity=40.3508771929825, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_GEOMG (Q39X89)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383860.1
- ProteinModelPortal:   Q39X89
- SMR:   Q39X89
- STRING:   Q39X89
- GeneID:   3738852
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0893
- NMPDR:   fig|269799.3.peg.968
- eggNOG:   COG1206
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- BioCyc:   GMET269799:GMET_0893-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 47145; Mature: 47013

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAITIIGGGLAGCEAVWQAAERGVRVTLHEMKPHAFSPAHHLAGLAELVCSNSLRGES
CCCEEEEEECCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCH
LENAVGLLKEELRRAGSLIMAAADATRVPAGGALAVDRELFSAFVTERIENHPLVDLMRG
HHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCC
EVADIPSEDIVIVATGPLTSDGLAERIRAITGPNLYFYDAIAPIVTAESLDMTKVFRASR
HHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCHHHHHHHHC
YGKGDGDDYLNCPLNQEEYEGFVDAILAAEKVPARDFEKVVHFEGCMPVEEMAERGRETL
CCCCCCCCCEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHCCHHHE
RFGPLKPVGLADPRTGEEPHAVVQLRAENREGTMFNLVGFQTKLTYPEQRRIFRMIPGLE
ECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECEEECCCHHHHHHHHHCCCCC
NAEFVRLGSMHRNTFINAPALLAPTFQLKGDARILFAGQITGVEGYVESAGSGFLAGVNA
CCCEEEECCHHHCCCCCCCHHCCCCEEECCCEEEEEEEEECCCHHHHHCCCCCEEECCCH
ARLVRGEEPVVPPLATALGALVTHITNADVKHFQPMNVNYGLFPPLEGKVKKKERRGKLA
HHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ERALAELDRWIGTV
HHHHHHHHHHHCCC
>Mature Secondary Structure 
TDAITIIGGGLAGCEAVWQAAERGVRVTLHEMKPHAFSPAHHLAGLAELVCSNSLRGES
CCEEEEEECCHHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCH
LENAVGLLKEELRRAGSLIMAAADATRVPAGGALAVDRELFSAFVTERIENHPLVDLMRG
HHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCC
EVADIPSEDIVIVATGPLTSDGLAERIRAITGPNLYFYDAIAPIVTAESLDMTKVFRASR
HHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCHHHHHHHHC
YGKGDGDDYLNCPLNQEEYEGFVDAILAAEKVPARDFEKVVHFEGCMPVEEMAERGRETL
CCCCCCCCCEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHCCHHHE
RFGPLKPVGLADPRTGEEPHAVVQLRAENREGTMFNLVGFQTKLTYPEQRRIFRMIPGLE
ECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECEEECCCHHHHHHHHHCCCCC
NAEFVRLGSMHRNTFINAPALLAPTFQLKGDARILFAGQITGVEGYVESAGSGFLAGVNA
CCCEEEECCHHHCCCCCCCHHCCCCEEECCCEEEEEEEEECCCHHHHHCCCCCEEECCCH
ARLVRGEEPVVPPLATALGALVTHITNADVKHFQPMNVNYGLFPPLEGKVKKKERRGKLA
HHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ERALAELDRWIGTV
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA