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The map label for this gene is topA [H]

Identifier: 78222111

GI number: 78222111

Start: 981935

End: 984211

Strand: Direct

Name: topA [H]

Synonym: Gmet_0891

Alternate gene names: 78222111

Gene position: 981935-984211 (Clockwise)

Preceding gene: 78222110

Following gene: 78222112

Centisome position: 24.56

GC content: 62.49

Gene sequence:

>2277_bases
ATGCCACAACACCTCGTCATCGTCGAATCTCCGGCCAAGGCCAAGACCATCGAGAAATTTCTCGGCAAGGACTTTCGGGT
CATGGCTTCCTACGGCCACGTACGCGCCCTCCCCAGCAAGCAGGGGTCCGTGGATGTCGCCAACGATTTCGAACCCAAAT
ATGCCATTCTCCCCGAGAGCAAGCGGCACATCGACGCCATCAAGAAGGAGCTGAAGGAGAGCGACATCCTGCTCTTGGCC
ACTGACCCCGACCGCGAAGGGGAGGCGATCTCCTGGCACCTGCTGGCGGCCCTCGGCATGGACAAAAAGAAAAACGCCGT
GCCGGTGAAGCGGGTCGTGTTCCACGAGATCACCAAGGACGCCATCGTCCACGCCGTGGAAAACCCCCGGGACATCTCCC
TGGAGTTGGTGGACGCCCAGCAGGCCCGCTCCATCCTTGACTACCTGGTCGGCTTCAACCTCTCCCCTTTCCTCTGGAAA
AAAATCCGCTACGGCCTCTCTGCCGGACGGGTCCAGTCCGTGGCCCTGCGGCTCATCTGCGAGCGGGAGAAGGAGATCAA
GGCTTTCCAGGAACAGGAGTACTGGACCATCGGCGCGGAGCTGGGCACCGGCAAGGGGCAGAAGTTCTCCGCCAACCTAG
TGGAGGCCGAGGGGAAGAAACTCGGCAAGTTCGATATCCCCGACGGCACGGCGGCCGACCGGCTCGTGAAGGCCCTGGAG
AACGGTGCCTACACCGTCGACAAAGTGGCGAAGAGCGAGCGCAAACGGACCCCAGCGCCGCCGTTCACCACCTCCACCCT
CCAGCAGGAGGCGGCAAGAAAGCTCGGCTTCTCGGCCAAGAAGACCATGTCCACGGCCCAGAAGCTCTACGAAGGGGTCG
CCGTGGGGGACGAGGGGCTTGTCGGTCTCATCACCTACATGCGTACCGACAGCGTGGTGCTGTCGAACCAGGCCCTCCAG
GAGGCCCACGAGGTCATCACCTCCCTCTACGGGAAGGAGTACGCCCTCGCCAAGCCCCGCTTTTACAAGAACAAGGCGAA
GAACGCCCAGGAGGCCCACGAGGCGGTCCGCCCCACCTCCATCGCCCGCACCCCGGTCGAGGTGAAGAAGTACCTCTCCT
CCGACCAGTTCAAACTCTACGATCTCATCTGGAAGCGGACCGTGGCCTGCCAGATGGCCGAGGCGCTTCTGGATCAGACC
TCTGTCGACATCGGCGCCGGCGAGGGCTATCGTTTCCGTGCCGCCGGCACCGTGATCCGCTTCCCCGGCTTCATGAAGCT
CTACATCGAGGGAGTCGACGACCAGGCCGAGGAGAAGGAAGGGACTCTGCCTCCCCTCACGGAAGGGGAACTCCTGAAGC
TCCAGAAGCTCGTTCCGGAGCAGCATTTCACCCAGCCCCCACCGCGGTACACCGAGGCGAGCCTCGTGAAGACCCTGGAG
GAGTACGGTATTGGCCGTCCCTCCACCTACGCCTCCATCATGAACACGCTCCTGGAGCGGAAATACGCCCGCCTCGACAG
CAAGCGCTTCGTTCCCGAGGATGTGGGGATGGTGGTGAACGATCTCCTCACCAACCACTTCACCCAGTACGTGGATTACA
ATTTCACCGCCAGCCTCGAAGAGGAGTTGGACCGGGTCTCCCGGGGTGAGAAGCAGTGGAAGCCCCTCCTTCATGACTTC
TGGGGCCCCTTTAGTTCCCTCCTGAAACAGAAAGAGGGGGAGGTGAGCAAGGCGGACCTCACCACCGAGGCCACCGACGA
ACTCTGCCCCGACTGCGGCAAGCCGCTGGTGGTGAAGCTTGGGAAGCGGGGGAAGTTCATCGCCTGCTCCGGCTACCAGG
AAGGGTGCACCTATACCCGCAACATCGCCCAGGGGGAGCAGCAGGAGCCGGCAGAACCGGTCTTCTCCGAGGAGAAGTGC
GACAAGTGCGGCAGCCCCATGCTCATCAAGGATGGCCGCTTCGGAAAGTACCTGGCCTGCTCCGCCTATCCCGCCTGCAA
GAACATCCAGCCCCTCGTAAAGCCCAAGGGGACCGGCCACACGTGCCCCGAGTGCAAGGAAGGGGAGCTGACCGAGAAGA
AGTCCCGCTACGGCAAGATGTTCTACTCCTGCAACCGCTACCCCCAGTGCAAGTTCGCCCTCTGGGATCCCCCCCAGCCG
GGGCCGTGCCCCAAATGTGGCTTCCCGCTCCTGGTGAAAAAGGTCTACAAGCGGGAAGGGGAGTTCCTCAAGTGCCCGAA
AGAGGGGTGTGACTACCGGACCGAAGGGAAGAAGTAG

Upstream 100 bases:

>100_bases
AACTCAAGGGGGCCGTAACTCAGCTTCCCGGCAAGTATTTTTGCACAAATTGACCCGCCTGGCATGCCAGGCGTTACTTA
CCTATTATTGGATTTTTTTT

Downstream 100 bases:

>100_bases
CCGAGAGACTGACCGGGCGGGGAGCGATCCCCGCCTTTTTAGCATGAAGCGGCAGTTTCAGTTTGAATAACACAGAGCAA
CTGAGCAACTGAGAAAACCC

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 758; Mature: 757

Protein sequence:

>758_residues
MPQHLVIVESPAKAKTIEKFLGKDFRVMASYGHVRALPSKQGSVDVANDFEPKYAILPESKRHIDAIKKELKESDILLLA
TDPDREGEAISWHLLAALGMDKKKNAVPVKRVVFHEITKDAIVHAVENPRDISLELVDAQQARSILDYLVGFNLSPFLWK
KIRYGLSAGRVQSVALRLICEREKEIKAFQEQEYWTIGAELGTGKGQKFSANLVEAEGKKLGKFDIPDGTAADRLVKALE
NGAYTVDKVAKSERKRTPAPPFTTSTLQQEAARKLGFSAKKTMSTAQKLYEGVAVGDEGLVGLITYMRTDSVVLSNQALQ
EAHEVITSLYGKEYALAKPRFYKNKAKNAQEAHEAVRPTSIARTPVEVKKYLSSDQFKLYDLIWKRTVACQMAEALLDQT
SVDIGAGEGYRFRAAGTVIRFPGFMKLYIEGVDDQAEEKEGTLPPLTEGELLKLQKLVPEQHFTQPPPRYTEASLVKTLE
EYGIGRPSTYASIMNTLLERKYARLDSKRFVPEDVGMVVNDLLTNHFTQYVDYNFTASLEEELDRVSRGEKQWKPLLHDF
WGPFSSLLKQKEGEVSKADLTTEATDELCPDCGKPLVVKLGKRGKFIACSGYQEGCTYTRNIAQGEQQEPAEPVFSEEKC
DKCGSPMLIKDGRFGKYLACSAYPACKNIQPLVKPKGTGHTCPECKEGELTEKKSRYGKMFYSCNRYPQCKFALWDPPQP
GPCPKCGFPLLVKKVYKREGEFLKCPKEGCDYRTEGKK

Sequences:

>Translated_758_residues
MPQHLVIVESPAKAKTIEKFLGKDFRVMASYGHVRALPSKQGSVDVANDFEPKYAILPESKRHIDAIKKELKESDILLLA
TDPDREGEAISWHLLAALGMDKKKNAVPVKRVVFHEITKDAIVHAVENPRDISLELVDAQQARSILDYLVGFNLSPFLWK
KIRYGLSAGRVQSVALRLICEREKEIKAFQEQEYWTIGAELGTGKGQKFSANLVEAEGKKLGKFDIPDGTAADRLVKALE
NGAYTVDKVAKSERKRTPAPPFTTSTLQQEAARKLGFSAKKTMSTAQKLYEGVAVGDEGLVGLITYMRTDSVVLSNQALQ
EAHEVITSLYGKEYALAKPRFYKNKAKNAQEAHEAVRPTSIARTPVEVKKYLSSDQFKLYDLIWKRTVACQMAEALLDQT
SVDIGAGEGYRFRAAGTVIRFPGFMKLYIEGVDDQAEEKEGTLPPLTEGELLKLQKLVPEQHFTQPPPRYTEASLVKTLE
EYGIGRPSTYASIMNTLLERKYARLDSKRFVPEDVGMVVNDLLTNHFTQYVDYNFTASLEEELDRVSRGEKQWKPLLHDF
WGPFSSLLKQKEGEVSKADLTTEATDELCPDCGKPLVVKLGKRGKFIACSGYQEGCTYTRNIAQGEQQEPAEPVFSEEKC
DKCGSPMLIKDGRFGKYLACSAYPACKNIQPLVKPKGTGHTCPECKEGELTEKKSRYGKMFYSCNRYPQCKFALWDPPQP
GPCPKCGFPLLVKKVYKREGEFLKCPKEGCDYRTEGKK
>Mature_757_residues
PQHLVIVESPAKAKTIEKFLGKDFRVMASYGHVRALPSKQGSVDVANDFEPKYAILPESKRHIDAIKKELKESDILLLAT
DPDREGEAISWHLLAALGMDKKKNAVPVKRVVFHEITKDAIVHAVENPRDISLELVDAQQARSILDYLVGFNLSPFLWKK
IRYGLSAGRVQSVALRLICEREKEIKAFQEQEYWTIGAELGTGKGQKFSANLVEAEGKKLGKFDIPDGTAADRLVKALEN
GAYTVDKVAKSERKRTPAPPFTTSTLQQEAARKLGFSAKKTMSTAQKLYEGVAVGDEGLVGLITYMRTDSVVLSNQALQE
AHEVITSLYGKEYALAKPRFYKNKAKNAQEAHEAVRPTSIARTPVEVKKYLSSDQFKLYDLIWKRTVACQMAEALLDQTS
VDIGAGEGYRFRAAGTVIRFPGFMKLYIEGVDDQAEEKEGTLPPLTEGELLKLQKLVPEQHFTQPPPRYTEASLVKTLEE
YGIGRPSTYASIMNTLLERKYARLDSKRFVPEDVGMVVNDLLTNHFTQYVDYNFTASLEEELDRVSRGEKQWKPLLHDFW
GPFSSLLKQKEGEVSKADLTTEATDELCPDCGKPLVVKLGKRGKFIACSGYQEGCTYTRNIAQGEQQEPAEPVFSEEKCD
KCGSPMLIKDGRFGKYLACSAYPACKNIQPLVKPKGTGHTCPECKEGELTEKKSRYGKMFYSCNRYPQCKFALWDPPQPG
PCPKCGFPLLVKKVYKREGEFLKCPKEGCDYRTEGKK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=585, Percent_Identity=24.7863247863248, Blast_Score=121, Evalue=3e-27,
Organism=Homo sapiens, GI4507635, Length=779, Percent_Identity=21.5661103979461, Blast_Score=108, Evalue=2e-23,
Organism=Escherichia coli, GI1787529, Length=766, Percent_Identity=38.2506527415144, Blast_Score=482, Evalue=1e-137,
Organism=Escherichia coli, GI48994931, Length=160, Percent_Identity=40, Blast_Score=136, Evalue=5e-33,
Organism=Escherichia coli, GI1788061, Length=554, Percent_Identity=24.5487364620939, Blast_Score=124, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17555378, Length=548, Percent_Identity=26.2773722627737, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32563869, Length=560, Percent_Identity=26.25, Blast_Score=114, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323263, Length=500, Percent_Identity=24.2, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585251, Length=673, Percent_Identity=23.9227340267459, Blast_Score=131, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 84932; Mature: 84801

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQHLVIVESPAKAKTIEKFLGKDFRVMASYGHVRALPSKQGSVDVANDFEPKYAILPES
CCCCEEEEECCHHHHHHHHHHCCCEEEEHHCCCEEECCCCCCCEECCCCCCCCEEECCCC
KRHIDAIKKELKESDILLLATDPDREGEAISWHLLAALGMDKKKNAVPVKRVVFHEITKD
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
AIVHAVENPRDISLELVDAQQARSILDYLVGFNLSPFLWKKIRYGLSAGRVQSVALRLIC
HHHHHHCCCCCCEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
EREKEIKAFQEQEYWTIGAELGTGKGQKFSANLVEAEGKKLGKFDIPDGTAADRLVKALE
HHHHHHHHHHHCCCEEEECCCCCCCCCEEECHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
NGAYTVDKVAKSERKRTPAPPFTTSTLQQEAARKLGFSAKKTMSTAQKLYEGVAVGDEGL
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCH
VGLITYMRTDSVVLSNQALQEAHEVITSLYGKEYALAKPRFYKNKAKNAQEAHEAVRPTS
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHCCCH
IARTPVEVKKYLSSDQFKLYDLIWKRTVACQMAEALLDQTSVDIGAGEGYRFRAAGTVIR
HCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEE
FPGFMKLYIEGVDDQAEEKEGTLPPLTEGELLKLQKLVPEQHFTQPPPRYTEASLVKTLE
CCCEEEEEEECCCCHHHHCCCCCCCCCCCHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHH
EYGIGRPSTYASIMNTLLERKYARLDSKRFVPEDVGMVVNDLLTNHFTQYVDYNFTASLE
HHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHH
EELDRVSRGEKQWKPLLHDFWGPFSSLLKQKEGEVSKADLTTEATDELCPDCGKPLVVKL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHCCCCCCCCEEEE
GKRGKFIACSGYQEGCTYTRNIAQGEQQEPAEPVFSEEKCDKCGSPMLIKDGRFGKYLAC
CCCCCEEEECCCHHCCCHHHHHHCCCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCEEEE
SAYPACKNIQPLVKPKGTGHTCPECKEGELTEKKSRYGKMFYSCNRYPQCKFALWDPPQP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCC
GPCPKCGFPLLVKKVYKREGEFLKCPKEGCDYRTEGKK
CCCCCCCHHHHHHHHHHCCCCEEECCHHCCCCCCCCCC
>Mature Secondary Structure 
PQHLVIVESPAKAKTIEKFLGKDFRVMASYGHVRALPSKQGSVDVANDFEPKYAILPES
CCCEEEEECCHHHHHHHHHHCCCEEEEHHCCCEEECCCCCCCEECCCCCCCCEEECCCC
KRHIDAIKKELKESDILLLATDPDREGEAISWHLLAALGMDKKKNAVPVKRVVFHEITKD
HHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
AIVHAVENPRDISLELVDAQQARSILDYLVGFNLSPFLWKKIRYGLSAGRVQSVALRLIC
HHHHHHCCCCCCEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
EREKEIKAFQEQEYWTIGAELGTGKGQKFSANLVEAEGKKLGKFDIPDGTAADRLVKALE
HHHHHHHHHHHCCCEEEECCCCCCCCCEEECHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
NGAYTVDKVAKSERKRTPAPPFTTSTLQQEAARKLGFSAKKTMSTAQKLYEGVAVGDEGL
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCH
VGLITYMRTDSVVLSNQALQEAHEVITSLYGKEYALAKPRFYKNKAKNAQEAHEAVRPTS
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHCCCH
IARTPVEVKKYLSSDQFKLYDLIWKRTVACQMAEALLDQTSVDIGAGEGYRFRAAGTVIR
HCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEE
FPGFMKLYIEGVDDQAEEKEGTLPPLTEGELLKLQKLVPEQHFTQPPPRYTEASLVKTLE
CCCEEEEEEECCCCHHHHCCCCCCCCCCCHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHH
EYGIGRPSTYASIMNTLLERKYARLDSKRFVPEDVGMVVNDLLTNHFTQYVDYNFTASLE
HHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHH
EELDRVSRGEKQWKPLLHDFWGPFSSLLKQKEGEVSKADLTTEATDELCPDCGKPLVVKL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHCCCCCCCCEEEE
GKRGKFIACSGYQEGCTYTRNIAQGEQQEPAEPVFSEEKCDKCGSPMLIKDGRFGKYLAC
CCCCCEEEECCCHHCCCHHHHHHCCCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCEEEE
SAYPACKNIQPLVKPKGTGHTCPECKEGELTEKKSRYGKMFYSCNRYPQCKFALWDPPQP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCC
GPCPKCGFPLLVKKVYKREGEFLKCPKEGCDYRTEGKK
CCCCCCCHHHHHHHHHHCCCCEEECCHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]