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The map label for this gene is usf [H]

Identifier: 78222010

GI number: 78222010

Start: 871620

End: 872429

Strand: Direct

Name: usf [H]

Synonym: Gmet_0790

Alternate gene names: 78222010

Gene position: 871620-872429 (Clockwise)

Preceding gene: 78222002

Following gene: 78222011

Centisome position: 21.8

GC content: 64.69

Gene sequence:

>810_bases
ATGGGCGGAATCAGAAAATTTGTCTGGGTGGCGGTCGCACTGGCCGGCGGCTTCCTGGGCGTGTCGACGGCGGATGCCGC
GGTGCAGGGGAAGGTGGTCGAGTACCGCGACGGCGAGGTGACCATGAAGGGGTATCTCGCCTGGAACGATACGGTGAAGG
GAAAACGTCCGGGTGTCCTTGTGGTGCACGAGTGGTGGGGGCTCAACGACTACGCCCGCAAGCGGGCGCGGATGCTGGCG
GAGCTGGGGTATACGGCCCTGGCGGTCGATATGTACGGCGGGGGGAAAGAAGCCACCCATCCCGACGATGCCGGCGCGTT
TTCCTCTGCGGTAATGAAGAACATGGACCTCATGGCTGCCCGCTTCCGGGCGGCGATGGAGTTTCTCAAGAAACAGCCCA
CCGTCGATGCCGTCCGCATCGGCGCCATCGGTTACTGCTTCGGCGGGGCCGTGGTGCTGAACATGGCCCGCCAGGGCCTT
GACCTCAAGGGGGTGGCCAGCTTCCACGGGAACCTCGCCACGGCAAAACCGGCCGGGCCGGGAACCGTCAAGGCAAAGGT
GACGGTTTTCAATGGCGGGGCCGACACCCTTGTGCCCCCCGAACAGGTGGGGGCCTTTACCGCTGAAATGACCAGGGCAG
GCGCCGCCTTCCGGTTCTTCTCCTACCCGGGCGCGAAACACGCCTTCACGAACCCCGACGCCGATGTCTATGCCCGGAAA
TTCGGCCTCCCTCTCGCCTATGACGCCGCCGCCGACAGGGATTCGTGGGAGGAGATGAAGCGCTTCTTCCGGGAAGTGTT
CGGACGATAG

Upstream 100 bases:

>100_bases
CGGAGGCGCATACGACGCCATGCGGGAAATTTCTCCGTAATCTGGTAAAGTTACCGCAGACGTTGGAACCGCCTTCGGGC
GGCGGGAGAGGAGGTGCGGC

Downstream 100 bases:

>100_bases
AGTCCCTTGCGACAACCGGATTGAAATTCACACGAACAGGATGGTCCACCTATGAACTCCATTCGCACGTCTCTGCTCCT
GGTTCTCACCGTCATCTCCC

Product: dienelactone hydrolase

Products: oxohex-2-enedioate [C]

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MGGIRKFVWVAVALAGGFLGVSTADAAVQGKVVEYRDGEVTMKGYLAWNDTVKGKRPGVLVVHEWWGLNDYARKRARMLA
ELGYTALAVDMYGGGKEATHPDDAGAFSSAVMKNMDLMAARFRAAMEFLKKQPTVDAVRIGAIGYCFGGAVVLNMARQGL
DLKGVASFHGNLATAKPAGPGTVKAKVTVFNGGADTLVPPEQVGAFTAEMTRAGAAFRFFSYPGAKHAFTNPDADVYARK
FGLPLAYDAAADRDSWEEMKRFFREVFGR

Sequences:

>Translated_269_residues
MGGIRKFVWVAVALAGGFLGVSTADAAVQGKVVEYRDGEVTMKGYLAWNDTVKGKRPGVLVVHEWWGLNDYARKRARMLA
ELGYTALAVDMYGGGKEATHPDDAGAFSSAVMKNMDLMAARFRAAMEFLKKQPTVDAVRIGAIGYCFGGAVVLNMARQGL
DLKGVASFHGNLATAKPAGPGTVKAKVTVFNGGADTLVPPEQVGAFTAEMTRAGAAFRFFSYPGAKHAFTNPDADVYARK
FGLPLAYDAAADRDSWEEMKRFFREVFGR
>Mature_268_residues
GGIRKFVWVAVALAGGFLGVSTADAAVQGKVVEYRDGEVTMKGYLAWNDTVKGKRPGVLVVHEWWGLNDYARKRARMLAE
LGYTALAVDMYGGGKEATHPDDAGAFSSAVMKNMDLMAARFRAAMEFLKKQPTVDAVRIGAIGYCFGGAVVLNMARQGLD
LKGVASFHGNLATAKPAGPGTVKAKVTVFNGGADTLVPPEQVGAFTAEMTRAGAAFRFFSYPGAKHAFTNPDADVYARKF
GLPLAYDAAADRDSWEEMKRFFREVFGR

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI48994982, Length=261, Percent_Identity=26.4367816091954, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17536419, Length=258, Percent_Identity=36.046511627907, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17532655, Length=248, Percent_Identity=36.6935483870968, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI193204305, Length=216, Percent_Identity=35.1851851851852, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17532653, Length=245, Percent_Identity=32.2448979591837, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17536415, Length=243, Percent_Identity=29.2181069958848, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17534829, Length=153, Percent_Identity=35.2941176470588, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: 3.1.1.45 [C]

Molecular weight: Translated: 28953; Mature: 28821

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGIRKFVWVAVALAGGFLGVSTADAAVQGKVVEYRDGEVTMKGYLAWNDTVKGKRPGVL
CCCHHHHHHHHHHHHCCHHCCCCCCHHHCCCEEEEECCCEEEEEEEEECCCCCCCCCCEE
VVHEWWGLNDYARKRARMLAELGYTALAVDMYGGGKEATHPDDAGAFSSAVMKNMDLMAA
EEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RFRAAMEFLKKQPTVDAVRIGAIGYCFGGAVVLNMARQGLDLKGVASFHGNLATAKPAGP
HHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCCCCCCCCCCC
GTVKAKVTVFNGGADTLVPPEQVGAFTAEMTRAGAAFRFFSYPGAKHAFTNPDADVYARK
CCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHH
FGLPLAYDAAADRDSWEEMKRFFREVFGR
HCCCEEECCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
GGIRKFVWVAVALAGGFLGVSTADAAVQGKVVEYRDGEVTMKGYLAWNDTVKGKRPGVL
CCHHHHHHHHHHHHCCHHCCCCCCHHHCCCEEEEECCCEEEEEEEEECCCCCCCCCCEE
VVHEWWGLNDYARKRARMLAELGYTALAVDMYGGGKEATHPDDAGAFSSAVMKNMDLMAA
EEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RFRAAMEFLKKQPTVDAVRIGAIGYCFGGAVVLNMARQGLDLKGVASFHGNLATAKPAGP
HHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEHHHHCCCCCCCCCCCC
GTVKAKVTVFNGGADTLVPPEQVGAFTAEMTRAGAAFRFFSYPGAKHAFTNPDADVYARK
CCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHH
FGLPLAYDAAADRDSWEEMKRFFREVFGR
HCCCEEECCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-carboxymethylenebut-2-en-4-olide; H2O [C]

Specific reaction: 4-carboxymethylenebut-2-en-4-olide + H2O = 4 oxohex-2-enedioate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7592443 [H]