Click here to switch to the map view.

The map label for this gene is zraS [H]

Identifier: 78221921

GI number: 78221921

Start: 770651

End: 772618

Strand: Direct

Name: zraS [H]

Synonym: Gmet_0701

Alternate gene names: 78221921

Gene position: 770651-772618 (Clockwise)

Preceding gene: 78221920

Following gene: 78221922

Centisome position: 19.28

GC content: 60.11

Gene sequence:

>1968_bases
GTGCGCCGCTATTTTTTTGCCTTCATGAACAACCTGAAGCTGCGCTGGAAGATGCTGGTGCTGGTGCTCCCCCTCGTGAC
GGTTCCGATCGTTCTGGTCGGTGGGGTCATCAGCTACATTTCAACCCAACAGGCGTACCGGGGGATCACCCAGACCAGCA
AGGACGACCTGGCCCACATGGCCAGTTTCACCATCGACCTCCTCAATTCCCACTACCAGCAGTTCCAGGTATACAAGCAG
GACAAGCAGCGGAGCACCAACCTGGAGCTGGCCACCCTCACCACCCTTGCCTACAACATGGTGGAGGCCCAGCATCGCCA
GTACAAGAGCGGCAAGGTCGACCTCCGGACCGCCAAGGAAGAGGCCCGCAAGGCCCTGAAACGGGTCAACGTGGGGACGA
CCGGCTACATCTACGCCATGTCCACCAAGGGGGATCTGGAGGTGCACATCGCCCGGGAGGGGGAGAACGTCTACGAGGAG
AAGGACGAGAACGGCCGCCCCTTCATCCGGATCATGTGCGAGAACGCCCTGAAGTCCAAGCCGGGGGAGGTCCTCTACAT
CGTCTACCCCTGGCGCAACCCGGTGCTCGGCGACAAGTATCCCCGCAACAAGGTGGTTGCCTACCGTTATTTCCGGGAGT
GGGACTGGATCATCGCCTCGGGGGGCTACCTGGAGGAGACCTACGAAGACCTCGCCTTTGAGCGCCGCTCTTTCCAGGAG
CTGAAGGATAAGATCAAGAGCAAGAAGGTGGGGGATACCGGCTACATTTTCTGCATGGACGACAAGGGGAACTTCACCGT
TCACCCCGGCGGGGAGGGGAAGAATTTCCTCAACGCCGTGGATTCCAGCGGCCACAAGTTCATCAAGGAAATGGTGGAGA
AGAAGCGGGGCTGGATCCGCTATCCCTGGCTCAACCCCGGGGAAAAGAACCCGCGGATGAAGATCGTCCGCTACGACTAC
TTCAAACCGTGGGGATGGATTGTGGCGGTCGGCTCCTACGAGGACGAGTTCTACGGGGAGGCCAACAAGATCCGGGGCCG
GATCGCAGCCTCCATGACGCTGCTGACCATTGTGACGAGTGTTGTGGCCACCGGGCTGGTCTTCCTGGCGGCCAAGATTT
TCACTCAGCCCATCATGCACATGATCGACGTGATCCGTAGGGTGAAGAAAGGGCGGCTCGACGAGCAGATGGAGGTGGAT
GCGGGGGATGAGCTGGGCGAGATGGCGTCGGCCTTTAACCTGATGACCGCCATGATCCGCCAGAACAAGGAGATGGAGGC
AAGCCTCGCCCAGCAGGGGAAGATGGCGTCCCTCGGTGTCCTCTCCTCGGGGGTGGCCCACGAGATCAACAACCCACTCG
GGGTTATCCTTGGCTACGCCTCCTACATCGAGGGGAAAACCAGCGAGGATGATCCCAACTACAGGTTCATCCACGAGATC
AAGCGGGAGAGCAAGCGGTGCAAGAAGATCGTCCAGGATCTCCTCTCCTATGCCCGCACCCCCAAGCCGGCCCTGGAGTG
GACCGACGTGAACGACCTGCTGGGGCAGATCGTCGATTTCGCCGCCAATCACACCGACATGCACCACGTGACCGTCGTGA
AGGATTTCTCTCCCGAATTGCCCCGGGTCATGGTCGACGGCGACCAGATCAGGCAGGTGGCCATCAACCTGATCCTGAAC
GCCGGAGCCGCCATGTCCGGGGAAGGGACCCTGACGGTGAAATCGTTCCTGGACGGCGAGGGGTACGTAAACATCGTCTT
CTGTGACAACGGGGCGGGAATACCCGCCGAGGACATCGAAAAAATTTTCGAGCCCTTCTTCACCACCAAGGCCAAGGGGA
CGGGGCTCGGCCTTGCCATCACGAAGCAGATCATCGAGATGCACCATGGCAAGATCAGCATCCTGAGCGAAGTGGGGAAG
GGGTCCGACGTGATTGTGAAGCTGCCGGTGGACCGGGACGAGCTGTGA

Upstream 100 bases:

>100_bases
CCCGTAACGCTTTCGCAATTCTGCAAATAATACTGGATTTTTTTATGACGTATACATAGTATGTCCTTTGACTTCGCTAA
CTTTCAGGACAGGGGATCCC

Downstream 100 bases:

>100_bases
CGGCCGGTAGGAACGAATTTTTTTCACAGGGGTAGAGAGTGTCCGAAAACAAGCGCATCATGCTCATCGACAACGAGGAG
GGGCTCTGCCGGATGATGGA

Product: Cache sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 655; Mature: 655

Protein sequence:

>655_residues
MRRYFFAFMNNLKLRWKMLVLVLPLVTVPIVLVGGVISYISTQQAYRGITQTSKDDLAHMASFTIDLLNSHYQQFQVYKQ
DKQRSTNLELATLTTLAYNMVEAQHRQYKSGKVDLRTAKEEARKALKRVNVGTTGYIYAMSTKGDLEVHIAREGENVYEE
KDENGRPFIRIMCENALKSKPGEVLYIVYPWRNPVLGDKYPRNKVVAYRYFREWDWIIASGGYLEETYEDLAFERRSFQE
LKDKIKSKKVGDTGYIFCMDDKGNFTVHPGGEGKNFLNAVDSSGHKFIKEMVEKKRGWIRYPWLNPGEKNPRMKIVRYDY
FKPWGWIVAVGSYEDEFYGEANKIRGRIAASMTLLTIVTSVVATGLVFLAAKIFTQPIMHMIDVIRRVKKGRLDEQMEVD
AGDELGEMASAFNLMTAMIRQNKEMEASLAQQGKMASLGVLSSGVAHEINNPLGVILGYASYIEGKTSEDDPNYRFIHEI
KRESKRCKKIVQDLLSYARTPKPALEWTDVNDLLGQIVDFAANHTDMHHVTVVKDFSPELPRVMVDGDQIRQVAINLILN
AGAAMSGEGTLTVKSFLDGEGYVNIVFCDNGAGIPAEDIEKIFEPFFTTKAKGTGLGLAITKQIIEMHHGKISILSEVGK
GSDVIVKLPVDRDEL

Sequences:

>Translated_655_residues
MRRYFFAFMNNLKLRWKMLVLVLPLVTVPIVLVGGVISYISTQQAYRGITQTSKDDLAHMASFTIDLLNSHYQQFQVYKQ
DKQRSTNLELATLTTLAYNMVEAQHRQYKSGKVDLRTAKEEARKALKRVNVGTTGYIYAMSTKGDLEVHIAREGENVYEE
KDENGRPFIRIMCENALKSKPGEVLYIVYPWRNPVLGDKYPRNKVVAYRYFREWDWIIASGGYLEETYEDLAFERRSFQE
LKDKIKSKKVGDTGYIFCMDDKGNFTVHPGGEGKNFLNAVDSSGHKFIKEMVEKKRGWIRYPWLNPGEKNPRMKIVRYDY
FKPWGWIVAVGSYEDEFYGEANKIRGRIAASMTLLTIVTSVVATGLVFLAAKIFTQPIMHMIDVIRRVKKGRLDEQMEVD
AGDELGEMASAFNLMTAMIRQNKEMEASLAQQGKMASLGVLSSGVAHEINNPLGVILGYASYIEGKTSEDDPNYRFIHEI
KRESKRCKKIVQDLLSYARTPKPALEWTDVNDLLGQIVDFAANHTDMHHVTVVKDFSPELPRVMVDGDQIRQVAINLILN
AGAAMSGEGTLTVKSFLDGEGYVNIVFCDNGAGIPAEDIEKIFEPFFTTKAKGTGLGLAITKQIIEMHHGKISILSEVGK
GSDVIVKLPVDRDEL
>Mature_655_residues
MRRYFFAFMNNLKLRWKMLVLVLPLVTVPIVLVGGVISYISTQQAYRGITQTSKDDLAHMASFTIDLLNSHYQQFQVYKQ
DKQRSTNLELATLTTLAYNMVEAQHRQYKSGKVDLRTAKEEARKALKRVNVGTTGYIYAMSTKGDLEVHIAREGENVYEE
KDENGRPFIRIMCENALKSKPGEVLYIVYPWRNPVLGDKYPRNKVVAYRYFREWDWIIASGGYLEETYEDLAFERRSFQE
LKDKIKSKKVGDTGYIFCMDDKGNFTVHPGGEGKNFLNAVDSSGHKFIKEMVEKKRGWIRYPWLNPGEKNPRMKIVRYDY
FKPWGWIVAVGSYEDEFYGEANKIRGRIAASMTLLTIVTSVVATGLVFLAAKIFTQPIMHMIDVIRRVKKGRLDEQMEVD
AGDELGEMASAFNLMTAMIRQNKEMEASLAQQGKMASLGVLSSGVAHEINNPLGVILGYASYIEGKTSEDDPNYRFIHEI
KRESKRCKKIVQDLLSYARTPKPALEWTDVNDLLGQIVDFAANHTDMHHVTVVKDFSPELPRVMVDGDQIRQVAINLILN
AGAAMSGEGTLTVKSFLDGEGYVNIVFCDNGAGIPAEDIEKIFEPFFTTKAKGTGLGLAITKQIIEMHHGKISILSEVGK
GSDVIVKLPVDRDEL

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=233, Percent_Identity=33.9055793991416, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1788549, Length=247, Percent_Identity=32.3886639676113, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1790300, Length=249, Percent_Identity=31.3253012048193, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI145693157, Length=253, Percent_Identity=29.2490118577075, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1788393, Length=286, Percent_Identity=24.4755244755245, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1790346, Length=305, Percent_Identity=25.9016393442623, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI48994928, Length=218, Percent_Identity=27.5229357798165, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1786912, Length=264, Percent_Identity=25.7575757575758, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI87081816, Length=350, Percent_Identity=25.7142857142857, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1789808, Length=301, Percent_Identity=24.5847176079734, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1786600, Length=217, Percent_Identity=27.6497695852535, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788713, Length=257, Percent_Identity=22.9571984435798, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1789149, Length=310, Percent_Identity=21.9354838709677, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 74139; Mature: 74139

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS00014 ER_TARGET ; PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRYFFAFMNNLKLRWKMLVLVLPLVTVPIVLVGGVISYISTQQAYRGITQTSKDDLAHM
CCHHHEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
ASFTIDLLNSHYQQFQVYKQDKQRSTNLELATLTTLAYNMVEAQHRQYKSGKVDLRTAKE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHH
EARKALKRVNVGTTGYIYAMSTKGDLEVHIAREGENVYEEKDENGRPFIRIMCENALKSK
HHHHHHHHCCCCCCEEEEEEECCCCEEEEEEECCCHHHHCCCCCCCCEEEEEEHHHHCCC
PGEVLYIVYPWRNPVLGDKYPRNKVVAYRYFREWDWIIASGGYLEETYEDLAFERRSFQE
CCCEEEEEECCCCCCCCCCCCCCCEEEEEEHHCCCEEEECCCCHHHHHHHHHHHHHHHHH
LKDKIKSKKVGDTGYIFCMDDKGNFTVHPGGEGKNFLNAVDSSGHKFIKEMVEKKRGWIR
HHHHHHHCCCCCCEEEEEEECCCCEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHCCEEE
YPWLNPGEKNPRMKIVRYDYFKPWGWIVAVGSYEDEFYGEANKIRGRIAASMTLLTIVTS
CCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHH
VVATGLVFLAAKIFTQPIMHMIDVIRRVKKGRLDEQMEVDAGDELGEMASAFNLMTAMIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
QNKEMEASLAQQGKMASLGVLSSGVAHEINNPLGVILGYASYIEGKTSEDDPNYRFIHEI
CCCHHHHHHHHCCCCHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHH
KRESKRCKKIVQDLLSYARTPKPALEWTDVNDLLGQIVDFAANHTDMHHVTVVKDFSPEL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCC
PRVMVDGDQIRQVAINLILNAGAAMSGEGTLTVKSFLDGEGYVNIVFCDNGAGIPAEDIE
CEEEECHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHH
KIFEPFFTTKAKGTGLGLAITKQIIEMHHGKISILSEVGKGSDVIVKLPVDRDEL
HHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEECCCCCCCC
>Mature Secondary Structure
MRRYFFAFMNNLKLRWKMLVLVLPLVTVPIVLVGGVISYISTQQAYRGITQTSKDDLAHM
CCHHHEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
ASFTIDLLNSHYQQFQVYKQDKQRSTNLELATLTTLAYNMVEAQHRQYKSGKVDLRTAKE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHH
EARKALKRVNVGTTGYIYAMSTKGDLEVHIAREGENVYEEKDENGRPFIRIMCENALKSK
HHHHHHHHCCCCCCEEEEEEECCCCEEEEEEECCCHHHHCCCCCCCCEEEEEEHHHHCCC
PGEVLYIVYPWRNPVLGDKYPRNKVVAYRYFREWDWIIASGGYLEETYEDLAFERRSFQE
CCCEEEEEECCCCCCCCCCCCCCCEEEEEEHHCCCEEEECCCCHHHHHHHHHHHHHHHHH
LKDKIKSKKVGDTGYIFCMDDKGNFTVHPGGEGKNFLNAVDSSGHKFIKEMVEKKRGWIR
HHHHHHHCCCCCCEEEEEEECCCCEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHCCEEE
YPWLNPGEKNPRMKIVRYDYFKPWGWIVAVGSYEDEFYGEANKIRGRIAASMTLLTIVTS
CCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHH
VVATGLVFLAAKIFTQPIMHMIDVIRRVKKGRLDEQMEVDAGDELGEMASAFNLMTAMIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
QNKEMEASLAQQGKMASLGVLSSGVAHEINNPLGVILGYASYIEGKTSEDDPNYRFIHEI
CCCHHHHHHHHCCCCHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHH
KRESKRCKKIVQDLLSYARTPKPALEWTDVNDLLGQIVDFAANHTDMHHVTVVKDFSPEL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCC
PRVMVDGDQIRQVAINLILNAGAAMSGEGTLTVKSFLDGEGYVNIVFCDNGAGIPAEDIE
CEEEECHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCHHHHH
KIFEPFFTTKAKGTGLGLAITKQIIEMHHGKISILSEVGKGSDVIVKLPVDRDEL
HHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11243806 [H]