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The map label for this gene is nifB [H]

Identifier: 78221901

GI number: 78221901

Start: 747698

End: 748570

Strand: Direct

Name: nifB [H]

Synonym: Gmet_0681

Alternate gene names: 78221901

Gene position: 747698-748570 (Clockwise)

Preceding gene: 78221894

Following gene: 78221902

Centisome position: 18.7

GC content: 64.15

Gene sequence:

>873_bases
ATGGCCACATCATGCGACCAGTTGAAGAAGATCCAGGGGCACCCCTGCTTCGGCGGCAACCACCACAAGAACGGCCGGAT
GCACCTGGCCGTGGCGCCCCGCTGCAACATCAAGTGCGGCTACTGCACCCGCAGGCACGACTGCGCCAACGAGTCCCGCC
CCGGCGTCACGAGCCGGCTTATCACTCCCGCCGAGGCCATCGCCAAGGTTCGCGAGGTGATGGCGAGCTCGGTGATGGGG
CCCATCATCAAGGTGATCGGCATTGCCGGCCCCGGCGACCCCTTGGCCAACGAAGAGACTTTCGAGACGTTCCGCCTCGT
GGGGGAAGAGTTCCCGCATCTCATGAAGTGCATGAGCACCAACGGGCTCCTCCTCCCTGACGCCATCGAGCGGCTCCACC
AACTGGACCTCCACAGCCTCACCGTTACCCTCAACGCCCTCGATCCGGCGGTGGGGGCGCGGATTTACGGACACATCAGC
TATCAAGGCCAGGTTTTTCGGGGCGTCGAGGCGGCGGAGATCCTGGTGGCCAACCAGCTGGAGGGGATCCGGCGGGCCGT
GGGATACGGGATGACGGTGAAGGTGAACACCGTCTACATCCCCGGGGTCAACGAGGAGCAGGTGCCCCTTATCGGAAGAA
AAATCAAGGAGCTCGGCGCCTTTGTCATGAATATAATGCCGCTCATACCCCAGGCTGATTTCGCCCATATCAGCCCGCCG
CCGCCCGAAGAGCTGGACCGGGTGCGGCAGCGAAACGAGTCGATCATCGGCCAGTTCAAGCACTGCCGCCAGTGCCGGGC
CGACGCCGTGGGGCTCATCGGCCGGGATGCCACCGTGACGGAGTCTGGCACTCCGCTTACCGTTGCCGTTTGA

Upstream 100 bases:

>100_bases
ACGACGAAGTGTGGAGAGGAAGACAGGCACAGGAGCCCGGCGCGAGAAATCCGCCGGGCTTTTTTTCGTTCAGGCAGCCC
ATTCGGGCACGGAGGCAGAG

Downstream 100 bases:

>100_bases
GGCTGGCATGAGGATCAGGCCCTTTTATCCCGATGACGTTTCCCTGTTTCTAGCCCTTGCCGCCGCCGAGGGGTGGATCA
GCGACCCGTGGGAGTTTGCA

Product: radical SAM family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MATSCDQLKKIQGHPCFGGNHHKNGRMHLAVAPRCNIKCGYCTRRHDCANESRPGVTSRLITPAEAIAKVREVMASSVMG
PIIKVIGIAGPGDPLANEETFETFRLVGEEFPHLMKCMSTNGLLLPDAIERLHQLDLHSLTVTLNALDPAVGARIYGHIS
YQGQVFRGVEAAEILVANQLEGIRRAVGYGMTVKVNTVYIPGVNEEQVPLIGRKIKELGAFVMNIMPLIPQADFAHISPP
PPEELDRVRQRNESIIGQFKHCRQCRADAVGLIGRDATVTESGTPLTVAV

Sequences:

>Translated_290_residues
MATSCDQLKKIQGHPCFGGNHHKNGRMHLAVAPRCNIKCGYCTRRHDCANESRPGVTSRLITPAEAIAKVREVMASSVMG
PIIKVIGIAGPGDPLANEETFETFRLVGEEFPHLMKCMSTNGLLLPDAIERLHQLDLHSLTVTLNALDPAVGARIYGHIS
YQGQVFRGVEAAEILVANQLEGIRRAVGYGMTVKVNTVYIPGVNEEQVPLIGRKIKELGAFVMNIMPLIPQADFAHISPP
PPEELDRVRQRNESIIGQFKHCRQCRADAVGLIGRDATVTESGTPLTVAV
>Mature_289_residues
ATSCDQLKKIQGHPCFGGNHHKNGRMHLAVAPRCNIKCGYCTRRHDCANESRPGVTSRLITPAEAIAKVREVMASSVMGP
IIKVIGIAGPGDPLANEETFETFRLVGEEFPHLMKCMSTNGLLLPDAIERLHQLDLHSLTVTLNALDPAVGARIYGHISY
QGQVFRGVEAAEILVANQLEGIRRAVGYGMTVKVNTVYIPGVNEEQVPLIGRKIKELGAFVMNIMPLIPQADFAHISPPP
PEELDRVRQRNESIIGQFKHCRQCRADAVGLIGRDATVTESGTPLTVAV

Specific function: Probably involved in the synthesis of the Fe-Mo cofactor [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaA/nifB/pqqE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR003731
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR005980
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02579 Nitro_FeMo-Co; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 31597; Mature: 31466

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATSCDQLKKIQGHPCFGGNHHKNGRMHLAVAPRCNIKCGYCTRRHDCANESRPGVTSRL
CCCCHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCEECCCCCCCCCCCCCCCCCHHHH
ITPAEAIAKVREVMASSVMGPIIKVIGIAGPGDPLANEETFETFRLVGEEFPHLMKCMST
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
NGLLLPDAIERLHQLDLHSLTVTLNALDPAVGARIYGHISYQGQVFRGVEAAEILVANQL
CCEECHHHHHHHHHCCHHEEEEEEECCCCHHCCEEEEEEEECCCEECCCHHHHHHHHHHH
EGIRRAVGYGMTVKVNTVYIPGVNEEQVPLIGRKIKELGAFVMNIMPLIPQADFAHISPP
HHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PPEELDRVRQRNESIIGQFKHCRQCRADAVGLIGRDATVTESGTPLTVAV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEC
>Mature Secondary Structure 
ATSCDQLKKIQGHPCFGGNHHKNGRMHLAVAPRCNIKCGYCTRRHDCANESRPGVTSRL
CCCHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCEECCCCCCCCCCCCCCCCCHHHH
ITPAEAIAKVREVMASSVMGPIIKVIGIAGPGDPLANEETFETFRLVGEEFPHLMKCMST
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
NGLLLPDAIERLHQLDLHSLTVTLNALDPAVGARIYGHISYQGQVFRGVEAAEILVANQL
CCEECHHHHHHHHHCCHHEEEEEEECCCCHHCCEEEEEEEECCCEECCCHHHHHHHHHHH
EGIRRAVGYGMTVKVNTVYIPGVNEEQVPLIGRKIKELGAFVMNIMPLIPQADFAHISPP
HHHHHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PPEELDRVRQRNESIIGQFKHCRQCRADAVGLIGRDATVTESGTPLTVAV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3062178; 3029020 [H]