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The map label for this gene is tuf1

Identifier: 78221844

GI number: 78221844

Start: 698775

End: 699965

Strand: Direct

Name: tuf1

Synonym: Gmet_0624

Alternate gene names: 78221844

Gene position: 698775-699965 (Clockwise)

Preceding gene: 78221843

Following gene: 78221845

Centisome position: 17.48

GC content: 58.86

Gene sequence:

>1191_bases
ATGGCAAAGGCAAAATTCGAGAGAACAAAACCGCACGTCAACATAGGGACGATTGGTCACGTTGACCATGGCAAGACCAC
GCTGACCGCCGCCATCACGCGGGTTCTGGCCGAGCGCGGCCAGGCCGAATTCAAGGGCTTTGATCAGATCGACAACGCCC
CTGAGGAGCGTGAGCGTGGTATCACCATCGCCACCTCGCACGTTGAGTATGAAACCGAAAAGCGTCACTATGCTCACGTT
GACTGTCCCGGCCACGCTGACTACGTCAAGAACATGATCACCGGTGCGGCTCAGATGGACGGCGCGATTCTCGTTGTCTC
CGCCGCCGACGGCCCCATGCCCCAGACCCGCGAGCACATTCTGCTTGCTCGTCAGGTAGGGGTTCCCTACATCGTTGTGT
TCCTTAACAAGGCTGACATGGTCGATGACGAAGAGCTTCTTGAGCTCGTCGAGCTGGAGATTCGCGAACTGCTCTCCTCC
TATGATTTCCCGGGCGACGACATTCCCATCATTAAGGGTTCCGCCCTCAAGGCCCTCAATGGCGACAAGGACGAGCTCGG
CTCTGAAGCCATCGTCAAGCTCATGGACGCCGTCGACGCCTATATTCCCGAGCCTGAGCGCGCCATCGACAAGCCGTTCC
TGATGCCCGTCGAAGACGTCTTTTCCATCTCCGGTCGCGGTACCGTTGCCACTGGTCGGGTTGAGCGTGGCGTTGTCAAG
GTGGGCGAGGAAGTCGAGATCGTTGGAATCAAGACGACCACCAAGACCACTGTTACCGGTGTAGAAATGTTCCGCAAGCT
CCTCGATGAAGGGCGTGCCGGCGACAACATCGGCGCCCTGCTCCGTGGTGTAAAGCGTGAAGACATCGAGCGTGGCCAGG
TGCTGGCGAGGCCGGGGAGCATCACCCCGCACACCAAGTTCAAGGCCGAGGCCTACATTCTGACCAAGGAAGAAGGCGGG
CGCCACACCCCGTTCTTCAACGGCTATCGTCCGCAGTTCTACTTTCGGACCACCGACGTGACCGGCATAGTAGACCTTCC
CGCAGGGACCGAAATGGTCATGCCCGGCGACAACGTAGCCGTAACGGTTAACCTGATCACCCCAATCGCCATGGACGAAG
GCCTGCGGTTCGCTATCCGTGAGGGTGGCCGTACTGTCGGCGCCGGTGTTGTCAGCTCTATTATCGAATAA

Upstream 100 bases:

>100_bases
ATGACGTTCGATCACTACGAGCAGGTTCCGAAATCGGTATCTGAAGAAATAATCGCTAAGGTAAAAGGGTAATTTATTTC
CATAGGGGGAGGAATCCTAC

Downstream 100 bases:

>100_bases
AGCAGGAACGAGGAAGAAATGCCAAGTCAAAAAATAAGAATCCGCCTTAAAGCTTTTGATCATAAGCTTCTTGATCAATC
AGTAGGTGAGATAGTCGATA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITRVLAERGQAEFKGFDQIDNAPEERERGITIATSHVEYETEKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDEELLELVELEIRELLSS
YDFPGDDIPIIKGSALKALNGDKDELGSEAIVKLMDAVDAYIPEPERAIDKPFLMPVEDVFSISGRGTVATGRVERGVVK
VGEEVEIVGIKTTTKTTVTGVEMFRKLLDEGRAGDNIGALLRGVKREDIERGQVLARPGSITPHTKFKAEAYILTKEEGG
RHTPFFNGYRPQFYFRTTDVTGIVDLPAGTEMVMPGDNVAVTVNLITPIAMDEGLRFAIREGGRTVGAGVVSSIIE

Sequences:

>Translated_396_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITRVLAERGQAEFKGFDQIDNAPEERERGITIATSHVEYETEKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDEELLELVELEIRELLSS
YDFPGDDIPIIKGSALKALNGDKDELGSEAIVKLMDAVDAYIPEPERAIDKPFLMPVEDVFSISGRGTVATGRVERGVVK
VGEEVEIVGIKTTTKTTVTGVEMFRKLLDEGRAGDNIGALLRGVKREDIERGQVLARPGSITPHTKFKAEAYILTKEEGG
RHTPFFNGYRPQFYFRTTDVTGIVDLPAGTEMVMPGDNVAVTVNLITPIAMDEGLRFAIREGGRTVGAGVVSSIIE
>Mature_395_residues
AKAKFERTKPHVNIGTIGHVDHGKTTLTAAITRVLAERGQAEFKGFDQIDNAPEERERGITIATSHVEYETEKRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDEELLELVELEIRELLSSY
DFPGDDIPIIKGSALKALNGDKDELGSEAIVKLMDAVDAYIPEPERAIDKPFLMPVEDVFSISGRGTVATGRVERGVVKV
GEEVEIVGIKTTTKTTVTGVEMFRKLLDEGRAGDNIGALLRGVKREDIERGQVLARPGSITPHTKFKAEAYILTKEEGGR
HTPFFNGYRPQFYFRTTDVTGIVDLPAGTEMVMPGDNVAVTVNLITPIAMDEGLRFAIREGGRTVGAGVVSSIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=58.3756345177665, Blast_Score=494, Evalue=1e-140,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=31.0267857142857, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=30.1339285714286, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI223555963, Length=445, Percent_Identity=30.561797752809, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI5729864, Length=445, Percent_Identity=30.561797752809, Blast_Score=171, Evalue=9e-43,
Organism=Homo sapiens, GI194097354, Length=433, Percent_Identity=27.9445727482679, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI194018522, Length=433, Percent_Identity=27.9445727482679, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI194018520, Length=433, Percent_Identity=27.9445727482679, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI46094014, Length=434, Percent_Identity=28.110599078341, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI54607086, Length=345, Percent_Identity=27.8260869565217, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI4503507, Length=268, Percent_Identity=30.5970149253731, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI82546879, Length=430, Percent_Identity=23.953488372093, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI157426893, Length=287, Percent_Identity=27.8745644599303, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=78.4810126582278, Blast_Score=620, Evalue=1e-179,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=78.4810126582278, Blast_Score=620, Evalue=1e-179,
Organism=Escherichia coli, GI2367247, Length=378, Percent_Identity=29.8941798941799, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI48994988, Length=252, Percent_Identity=32.9365079365079, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1789108, Length=323, Percent_Identity=31.2693498452012, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788922, Length=145, Percent_Identity=33.1034482758621, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=51.8796992481203, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=45.9948320413437, Blast_Score=353, Evalue=9e-98,
Organism=Caenorhabditis elegans, GI17552884, Length=445, Percent_Identity=29.6629213483146, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17569207, Length=445, Percent_Identity=29.6629213483146, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI32566303, Length=430, Percent_Identity=29.0697674418605, Blast_Score=147, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI32566629, Length=351, Percent_Identity=29.6296296296296, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI115532067, Length=436, Percent_Identity=27.5229357798165, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=436, Percent_Identity=27.5229357798165, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=299, Percent_Identity=29.0969899665552, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI32566301, Length=145, Percent_Identity=40, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17509919, Length=285, Percent_Identity=28.4210526315789, Blast_Score=85, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=233, Percent_Identity=29.6137339055794, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=66.5829145728643, Blast_Score=543, Evalue=1e-155,
Organism=Saccharomyces cerevisiae, GI6325337, Length=443, Percent_Identity=30.2483069977427, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6319594, Length=443, Percent_Identity=30.2483069977427, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6322937, Length=455, Percent_Identity=24.6153846153846, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320377, Length=337, Percent_Identity=30.8605341246291, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6320863, Length=282, Percent_Identity=29.0780141843972, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323320, Length=243, Percent_Identity=30.8641975308642, Blast_Score=73, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=190, Percent_Identity=33.6842105263158, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=59.5419847328244, Blast_Score=496, Evalue=1e-141,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=59.5419847328244, Blast_Score=496, Evalue=1e-141,
Organism=Drosophila melanogaster, GI19921738, Length=385, Percent_Identity=50.3896103896104, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI45553807, Length=444, Percent_Identity=30.6306306306306, Blast_Score=175, Evalue=5e-44,
Organism=Drosophila melanogaster, GI45553816, Length=444, Percent_Identity=30.6306306306306, Blast_Score=175, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24651721, Length=444, Percent_Identity=30.6306306306306, Blast_Score=175, Evalue=5e-44,
Organism=Drosophila melanogaster, GI17864154, Length=444, Percent_Identity=30.6306306306306, Blast_Score=175, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24652838, Length=450, Percent_Identity=30.4444444444444, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI17137572, Length=450, Percent_Identity=30.4444444444444, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=31.4754098360656, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI45550900, Length=442, Percent_Identity=28.2805429864253, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17137380, Length=340, Percent_Identity=30, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24647046, Length=281, Percent_Identity=29.1814946619217, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24647048, Length=281, Percent_Identity=29.1814946619217, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28572034, Length=179, Percent_Identity=35.7541899441341, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_GEOMG (Q39Y08)

Other databases:

- EMBL:   CP000148
- EMBL:   CP000148
- RefSeq:   YP_383578.1
- RefSeq:   YP_383591.1
- HSSP:   P0A6N1
- ProteinModelPortal:   Q39Y08
- SMR:   Q39Y08
- STRING:   Q39Y08
- GeneID:   3738962
- GeneID:   3739247
- GenomeReviews:   CP000148_GR
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0611
- KEGG:   gme:Gmet_0624
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- PhylomeDB:   Q39Y08
- BioCyc:   GMET269799:GMET_0611-MONOMER
- BioCyc:   GMET269799:GMET_0624-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43208; Mature: 43077

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERTKPHVNIGTIGHVDHGKTTLTAAITRVLAERGQAEFKGFDQIDNAPEERERG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCHHHCCCHHHCCCHHHHHCC
ITIATSHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEECEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIVVFLNKADMVDDEELLELVELEIRELLSSYDFPGDDIPIIKGSALKALN
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEECCCC
GDKDELGSEAIVKLMDAVDAYIPEPERAIDKPFLMPVEDVFSISGRGTVATGRVERGVVK
CCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCEEEECCHHHHHHC
VGEEVEIVGIKTTTKTTVTGVEMFRKLLDEGRAGDNIGALLRGVKREDIERGQVLARPGS
CCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEECCCC
ITPHTKFKAEAYILTKEEGGRHTPFFNGYRPQFYFRTTDVTGIVDLPAGTEMVMPGDNVA
CCCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCEEEECCCCEE
VTVNLITPIAMDEGLRFAIREGGRTVGAGVVSSIIE
EEEEEECCEECCCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKAKFERTKPHVNIGTIGHVDHGKTTLTAAITRVLAERGQAEFKGFDQIDNAPEERERG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCHHHCCCHHHCCCHHHHHCC
ITIATSHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEECEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIVVFLNKADMVDDEELLELVELEIRELLSSYDFPGDDIPIIKGSALKALN
HHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEECCCC
GDKDELGSEAIVKLMDAVDAYIPEPERAIDKPFLMPVEDVFSISGRGTVATGRVERGVVK
CCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHCCCCCCEEEECCHHHHHHC
VGEEVEIVGIKTTTKTTVTGVEMFRKLLDEGRAGDNIGALLRGVKREDIERGQVLARPGS
CCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEECCCC
ITPHTKFKAEAYILTKEEGGRHTPFFNGYRPQFYFRTTDVTGIVDLPAGTEMVMPGDNVA
CCCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCEEEECCCCEE
VTVNLITPIAMDEGLRFAIREGGRTVGAGVVSSIIE
EEEEEECCEECCCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA