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The map label for this gene is zraS [H]

Identifier: 78221775

GI number: 78221775

Start: 591018

End: 592517

Strand: Direct

Name: zraS [H]

Synonym: Gmet_0554

Alternate gene names: 78221775

Gene position: 591018-592517 (Clockwise)

Preceding gene: 78221774

Following gene: 78221776

Centisome position: 14.78

GC content: 59.2

Gene sequence:

>1500_bases
TTGATCTTCAAAAGCCTGACAACGAGAGTTATCGTAACCCTGGTCGGCCTCCTTTCCGTGGGAATCGGCACCTTTACCTA
CTTCAACCTCAAACGGGAAAAAGACCAGCTTGTCAAGGCAACCCGCGAGAATGCCTACCTCCTTCTCGACACTATCGAGC
GCAGCATCTACAAGTCCATGAGCGTTGGTGACACCCAGGATGTGCAGGTCATGCTGCAGATGGTGGGCCATAGCCACAGC
AAACTCATCGGCGTCAGGATATTTCATCCCCAGGGAATCGTCCTCAAGTCGTCCCGGCCCGAGGAGGTGGGGATGCCGGT
GCCCGAGGGGGATTACCAACTCTTTATCAACAACCGCAAGGAAGGTGTCTACTCCAGCGACGCCTTCGGTGAGGTCCTCG
GGGTTATCAAGCCGATCTACAACGATGTGCTGTGCCAACGGTGCCACGGCACCAAAAAGCGGCTTATCGGGGTTCTCAAC
GTCAACTACTCCCAGGTCGAGACCAACCGCCGGATTCTTGAGGCAACGCGGCTTTCCCTCGTCTCCACCGGTGCGGTCAT
TCTCTTCTGCGCGGCAGCCATCTCCCTCGTCATCGTCCGATTTGTCCGGCAACCCTTGAACAACCTCATCGACAGCATGG
CGCGGGTGGAGGAGGGAGACCTTTCCGTCCGCATAACCCCTGTGGCCGAGGACGAGATCGGCCGGCTCATCGTGAGTTTC
GACTCCATGGTCGGCAAGCTCGACAAGGCGAAGAGGGAACTGGAAGAGTACCACTTCCAGCAGATGGAACGGGCCGATCG
TCTGGCCTCGGTGGGGGAAATGGCCGCGGGGATCGCTCACGAAATCAAGAACCCCCTGGCCGGCATCTCGGCGGCCATGA
CCATCATCCGTCAGGATTTTTCCGACGAAGATCCCCGCAAGGAGATCATCGGAGAAGTGGTCGAGCAGGTGAGCCGGCTC
GACAAGACGGTGAACGATCTCCTGTTCTTCGGCAAGCCGACCCCCCCCGAACCGACCTATGCCGACGTGAACGCGATCCT
GCGGAAAACCCTCCTCTTCGCCCTGCAGCACAAGGGGGGAAAGAATATCGCCAAGAGCCTGGATCTTGCCGACGGCCTTC
CGGAAATCTTCGTGGACCCGAAGCAGGTGCAGCAGGTTTTCCTGAATCTGATCCTTAACGCCATCCAGGCCATGCAGGCC
GACGGGGGGACCCTCACCGTGGGGAGTTCCCTGGTGGACCTTGACGGCAAGGGGTGGGTGCGCACCACCATTGCCGACAC
TGGCCCCGGCATCCCGGAGCCGATCATCGGCAAAATCTTTACCCCCTTCTTCACCACCAAGGCACAAGGGACGGGGCTTG
GACTCGCCATCTGCCACAAGCTCATTACCCAGCATGGCGGTACCCTTTCCGTGACCAGCGAAGACGGCAAAGGAACGGTG
TTCACCATCGATCTGCCGGTGGCGGCATACGAAGAGGGTGCGCCGGAAGAAGAGGCATAG

Upstream 100 bases:

>100_bases
TGGCATCGCCCTTCTGGTGGAGCGGCATGCAGTGGATTGACATCAGCGCAGCGCAGTTGCCCCTGCAAGGATTCCCCGGC
TCCGCCACCTCGGAGGTAGG

Downstream 100 bases:

>100_bases
TGGCCGGAGTCGGTCAGGTTTTATTCTGGATTCTGGCTCCTGTATTCTGTATTCTTTTTTCATTCGGTAATTTCCAGATA
TCCGCTTGGTTACGATAAAG

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MIFKSLTTRVIVTLVGLLSVGIGTFTYFNLKREKDQLVKATRENAYLLLDTIERSIYKSMSVGDTQDVQVMLQMVGHSHS
KLIGVRIFHPQGIVLKSSRPEEVGMPVPEGDYQLFINNRKEGVYSSDAFGEVLGVIKPIYNDVLCQRCHGTKKRLIGVLN
VNYSQVETNRRILEATRLSLVSTGAVILFCAAAISLVIVRFVRQPLNNLIDSMARVEEGDLSVRITPVAEDEIGRLIVSF
DSMVGKLDKAKRELEEYHFQQMERADRLASVGEMAAGIAHEIKNPLAGISAAMTIIRQDFSDEDPRKEIIGEVVEQVSRL
DKTVNDLLFFGKPTPPEPTYADVNAILRKTLLFALQHKGGKNIAKSLDLADGLPEIFVDPKQVQQVFLNLILNAIQAMQA
DGGTLTVGSSLVDLDGKGWVRTTIADTGPGIPEPIIGKIFTPFFTTKAQGTGLGLAICHKLITQHGGTLSVTSEDGKGTV
FTIDLPVAAYEEGAPEEEA

Sequences:

>Translated_499_residues
MIFKSLTTRVIVTLVGLLSVGIGTFTYFNLKREKDQLVKATRENAYLLLDTIERSIYKSMSVGDTQDVQVMLQMVGHSHS
KLIGVRIFHPQGIVLKSSRPEEVGMPVPEGDYQLFINNRKEGVYSSDAFGEVLGVIKPIYNDVLCQRCHGTKKRLIGVLN
VNYSQVETNRRILEATRLSLVSTGAVILFCAAAISLVIVRFVRQPLNNLIDSMARVEEGDLSVRITPVAEDEIGRLIVSF
DSMVGKLDKAKRELEEYHFQQMERADRLASVGEMAAGIAHEIKNPLAGISAAMTIIRQDFSDEDPRKEIIGEVVEQVSRL
DKTVNDLLFFGKPTPPEPTYADVNAILRKTLLFALQHKGGKNIAKSLDLADGLPEIFVDPKQVQQVFLNLILNAIQAMQA
DGGTLTVGSSLVDLDGKGWVRTTIADTGPGIPEPIIGKIFTPFFTTKAQGTGLGLAICHKLITQHGGTLSVTSEDGKGTV
FTIDLPVAAYEEGAPEEEA
>Mature_499_residues
MIFKSLTTRVIVTLVGLLSVGIGTFTYFNLKREKDQLVKATRENAYLLLDTIERSIYKSMSVGDTQDVQVMLQMVGHSHS
KLIGVRIFHPQGIVLKSSRPEEVGMPVPEGDYQLFINNRKEGVYSSDAFGEVLGVIKPIYNDVLCQRCHGTKKRLIGVLN
VNYSQVETNRRILEATRLSLVSTGAVILFCAAAISLVIVRFVRQPLNNLIDSMARVEEGDLSVRITPVAEDEIGRLIVSF
DSMVGKLDKAKRELEEYHFQQMERADRLASVGEMAAGIAHEIKNPLAGISAAMTIIRQDFSDEDPRKEIIGEVVEQVSRL
DKTVNDLLFFGKPTPPEPTYADVNAILRKTLLFALQHKGGKNIAKSLDLADGLPEIFVDPKQVQQVFLNLILNAIQAMQA
DGGTLTVGSSLVDLDGKGWVRTTIADTGPGIPEPIIGKIFTPFFTTKAQGTGLGLAICHKLITQHGGTLSVTSEDGKGTV
FTIDLPVAAYEEGAPEEEA

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=231, Percent_Identity=38.961038961039, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1788549, Length=264, Percent_Identity=32.9545454545455, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1790300, Length=250, Percent_Identity=30.8, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1788393, Length=300, Percent_Identity=27.3333333333333, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI87081816, Length=349, Percent_Identity=28.3667621776504, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789149, Length=339, Percent_Identity=26.2536873156342, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI145693157, Length=259, Percent_Identity=31.2741312741313, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786783, Length=324, Percent_Identity=24.6913580246914, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1788713, Length=237, Percent_Identity=26.5822784810127, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI48994928, Length=237, Percent_Identity=29.1139240506329, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1786912, Length=294, Percent_Identity=26.530612244898, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790346, Length=233, Percent_Identity=26.6094420600858, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1790861, Length=359, Percent_Identity=24.7910863509749, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1789808, Length=306, Percent_Identity=23.202614379085, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 54648; Mature: 54648

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFKSLTTRVIVTLVGLLSVGIGTFTYFNLKREKDQLVKATRENAYLLLDTIERSIYKSM
CCHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHCCCEEEHHHHHHHHHHHC
SVGDTQDVQVMLQMVGHSHSKLIGVRIFHPQGIVLKSSRPEEVGMPVPEGDYQLFINNRK
CCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCHHCCCCCCCCCEEEEEECCC
EGVYSSDAFGEVLGVIKPIYNDVLCQRCHGTKKRLIGVLNVNYSQVETNRRILEATRLSL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCHHHHHHHHHHHHHHHHHH
VSTGAVILFCAAAISLVIVRFVRQPLNNLIDSMARVEEGDLSVRITPVAEDEIGRLIVSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHH
DSMVGKLDKAKRELEEYHFQQMERADRLASVGEMAAGIAHEIKNPLAGISAAMTIIRQDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCC
SDEDPRKEIIGEVVEQVSRLDKTVNDLLFFGKPTPPEPTYADVNAILRKTLLFALQHKGG
CCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
KNIAKSLDLADGLPEIFVDPKQVQQVFLNLILNAIQAMQADGGTLTVGSSLVDLDGKGWV
HHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEECCCCCEE
RTTIADTGPGIPEPIIGKIFTPFFTTKAQGTGLGLAICHKLITQHGGTLSVTSEDGKGTV
EEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEE
FTIDLPVAAYEEGAPEEEA
EEEECCHHHHCCCCCCCCC
>Mature Secondary Structure
MIFKSLTTRVIVTLVGLLSVGIGTFTYFNLKREKDQLVKATRENAYLLLDTIERSIYKSM
CCHHHHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHCCCEEEHHHHHHHHHHHC
SVGDTQDVQVMLQMVGHSHSKLIGVRIFHPQGIVLKSSRPEEVGMPVPEGDYQLFINNRK
CCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEECCCCHHCCCCCCCCCEEEEEECCC
EGVYSSDAFGEVLGVIKPIYNDVLCQRCHGTKKRLIGVLNVNYSQVETNRRILEATRLSL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEECCHHHHHHHHHHHHHHHHHH
VSTGAVILFCAAAISLVIVRFVRQPLNNLIDSMARVEEGDLSVRITPVAEDEIGRLIVSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHH
DSMVGKLDKAKRELEEYHFQQMERADRLASVGEMAAGIAHEIKNPLAGISAAMTIIRQDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCC
SDEDPRKEIIGEVVEQVSRLDKTVNDLLFFGKPTPPEPTYADVNAILRKTLLFALQHKGG
CCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
KNIAKSLDLADGLPEIFVDPKQVQQVFLNLILNAIQAMQADGGTLTVGSSLVDLDGKGWV
HHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEEECCCCCEE
RTTIADTGPGIPEPIIGKIFTPFFTTKAQGTGLGLAICHKLITQHGGTLSVTSEDGKGTV
EEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEE
FTIDLPVAAYEEGAPEEEA
EEEECCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]