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The map label for this gene is metH [H]

Identifier: 78221770

GI number: 78221770

Start: 585401

End: 587815

Strand: Direct

Name: metH [H]

Synonym: Gmet_0549

Alternate gene names: 78221770

Gene position: 585401-587815 (Clockwise)

Preceding gene: 78221769

Following gene: 78221771

Centisome position: 14.64

GC content: 68.24

Gene sequence:

>2415_bases
ATGAAAACACCCTTTCTGCAGGCGATCAAAGAACGGGTCCTGGTCCTTGACGGGGCCATGGGGACCATGCTCCAGGAGCG
GGGGCTGCGCCCCGGCCAGTCCCCCGAAGAGCTGAACCTCACCATGCCCGACGTGGTGGCCGGGGTCCATCGGGAATATC
TGGAGGCTGGGGCCGACATCATCGTCACCAACACCTTCGGCGGCACCCGCCCCAAGCTGGAGCACTACGGCCTGGAGGAG
AAGGTCCGCGAGATCAACGCCCGGGCCGTGGAGATCGCCCGGGAGGTTTGCGGCGACCGGGCCTACGTGGCCGCCTCCAT
GGGGCCCACGGGGCTCTTCGTGGAGCCGGTGGGGGAGGCGACCTTCGACGCCATGGCCGCCTTCTTCCGGGAACAGGCCG
CGGCCCTCATCGAAGCCGGCGCCGACCTCATCACCCTGGAGACCTTCCTCGACATCAAGGAGATCCGGGCAGCGGTCATC
GCCATCCGTGAGGTCTCTCCCACGATCCCCGTCATCGCCCAGCTCACCTTCGACAACGAGGGACGCACTGTCCTCGGCAC
CCCCCCCGAAGCGGCGGCCGTGACCCTAGCGGCGGCCGGGGCCGACATCGTCGGCTCCAACTGCGGTCTCGGTCCCGATG
GCATCTGCGCCGTCCTGGGAGCCATGCGTGGGGTGACCCGGCTTCCCCTCATTTCCCAGGCCAACGCGGGGCTGCCGAAG
CTGGTGGACGGCAAGACCGTCTTCCCCGGCACCCCCGACGACATGACCGCCTTCCACGACCGGATGCTGGGACTGAACGT
GCGGGTCATTGGCGGTTGCTGCGGCACCACCCCGACCCACATCCGGGCCATCAAGGAGGCCCTGGCGGGGCGCGACCAGT
CGTGGCGCGACTGCGGCCCCCCGGCCGGGGTCACGTTCCTGGCGAGCCGCACCTCGGTCGTCGCCCTCGGCGGGGGCCTT
CCCGCGGCCATCATCGGCGAGCGGATCAACCCCACCGGCAAGAAGGCCTTTGCCCAGGAGCTGCGGGAGGGGAAGATTAC
CACCATCCGCCGCGAGGCCATGGAACAGACGGCTGCCGGCGCCCACCTCCTGGACGTGAACGTGGGGACCCCGGGGATCG
ACGAGCCTGCCGCCATGGAGCGGGCGGTCTTCTGTGTTACCGGAAGCGTCGCCGTGCCGCTGGTCCTCGATTCTTCCGAT
CCGGCGGCCCTGGAACGGGGACTCAAGGCGGCCGACGGCAAGGTTCTCGTGAACTCCGTGAACGGCGAGACGAAGAGCAT
CGAGCGGGTCCTCCCCCTGGTGAAGAAGTACGGCGCCGCCGTCATCGGCCTGGCCCTGGACGAGACCGGTATCCCCGAGA
CCGCCGAGGGGCGCCTGGCCGTGGCGGAACGGATCGTGGCCGCTGCGGAAGGGCGCGGCATCTCCCGCAACGACGTGGTG
ATTGATTGCCTGACCCTCACCGTGAGCGCTGAGCAGAAGCGGGCCATGGAAACCCTGCGGGCGGTGCGCCTCGTGAAGGA
GATCATTGGCTGCCCCACGGTCCTCGGCGTCAGCAACATCTCCTTCGGCCTTCCCCGCCGTCCCCTCATCTCTTCGGCCT
TCTTCGCCATGGCCTTGGCCGCGGGGCTCGACGCCGCCATCGTCAACCCGAAGGAGGAGGAGATGATGGCGGCCTGGCGT
TCGGCCATGGTCCTCCTGAACCGGGACCCCAGCGCTGCCGCGTACATCGCTGCCTACGCCGGGACGGCCGCCGCTGCCCC
TGAACCGGCAGCGTCCGGCGAGGCCCTCGACATTCGGGAGCGCTTGAAGCGTGCCGTCATCACCGGCGACAGGGAGAATA
TCGTTGCGTTGGTTGAGGAGGCGCTTGGCCAGGGGCTCGAACCGCTCCAGGTGAGCAACGAAGGGCTCCTCCCCGGCCTG
GAGGAGGTGGGGCGGCGCTTCGAGAAGAACCTCGTCTTCCTTCCCCAGGTGATGCAGTCGGCCGACACGATGCAGGCCGC
CTTCGGCCGCCTCAGGGAGGTCATGAAGGGTTCTCCCGCCGCCAGCCGGGGGAAGATCCTCATGGCCACCGTCGAGGGGG
ACATCCACGATATCGGCAAGAATATCGTCTGCACGCTCCTGGAGAACCACGGCTTCGAGGTGATCGATCTGGGGAAGAAC
GTCCCTGCCGACCGGATCGTGGGAAAGGCCCGGGAATTGGGCGTCGATGCCGTGGGGCTCTCGGCCCTCATGACCACCAC
CATGACCGAGATGGAGAATGTGATTGCGCGGCTGAAGGAGGCCGGCATCCGGACGTTCACCATGGTGGGGGGGGCGGTGG
TGACCCAGGAGTACGCCGACGAGATTGGCGCCGATCTCTACGCCCGGGACGCCATGGAGGCGGTGGCGCGGATCAAGGCG
CTGCTGGAGAAGTAG

Upstream 100 bases:

>100_bases
ACGGCGCAGAGGTTGAGACCCTTACGGGACACCTGGACCTGCGCCTTCCCATCCCCCCCGATTTCTCCCTGGAAAAAAAT
AACAAAAAGGTGGCGACGCC

Downstream 100 bases:

>100_bases
GCGTAACTGGCCGATTCGGCTGGGTATTTTCAGTCCTTGCAACCGTGGGGCTATCCGTGATAGTTTTAATCGGTTACGTC
ATTTGACGGACAGGTGCCCC

Product: methionine synthase (B12-dependent)

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 804; Mature: 804

Protein sequence:

>804_residues
MKTPFLQAIKERVLVLDGAMGTMLQERGLRPGQSPEELNLTMPDVVAGVHREYLEAGADIIVTNTFGGTRPKLEHYGLEE
KVREINARAVEIAREVCGDRAYVAASMGPTGLFVEPVGEATFDAMAAFFREQAAALIEAGADLITLETFLDIKEIRAAVI
AIREVSPTIPVIAQLTFDNEGRTVLGTPPEAAAVTLAAAGADIVGSNCGLGPDGICAVLGAMRGVTRLPLISQANAGLPK
LVDGKTVFPGTPDDMTAFHDRMLGLNVRVIGGCCGTTPTHIRAIKEALAGRDQSWRDCGPPAGVTFLASRTSVVALGGGL
PAAIIGERINPTGKKAFAQELREGKITTIRREAMEQTAAGAHLLDVNVGTPGIDEPAAMERAVFCVTGSVAVPLVLDSSD
PAALERGLKAADGKVLVNSVNGETKSIERVLPLVKKYGAAVIGLALDETGIPETAEGRLAVAERIVAAAEGRGISRNDVV
IDCLTLTVSAEQKRAMETLRAVRLVKEIIGCPTVLGVSNISFGLPRRPLISSAFFAMALAAGLDAAIVNPKEEEMMAAWR
SAMVLLNRDPSAAAYIAAYAGTAAAAPEPAASGEALDIRERLKRAVITGDRENIVALVEEALGQGLEPLQVSNEGLLPGL
EEVGRRFEKNLVFLPQVMQSADTMQAAFGRLREVMKGSPAASRGKILMATVEGDIHDIGKNIVCTLLENHGFEVIDLGKN
VPADRIVGKARELGVDAVGLSALMTTTMTEMENVIARLKEAGIRTFTMVGGAVVTQEYADEIGADLYARDAMEAVARIKA
LLEK

Sequences:

>Translated_804_residues
MKTPFLQAIKERVLVLDGAMGTMLQERGLRPGQSPEELNLTMPDVVAGVHREYLEAGADIIVTNTFGGTRPKLEHYGLEE
KVREINARAVEIAREVCGDRAYVAASMGPTGLFVEPVGEATFDAMAAFFREQAAALIEAGADLITLETFLDIKEIRAAVI
AIREVSPTIPVIAQLTFDNEGRTVLGTPPEAAAVTLAAAGADIVGSNCGLGPDGICAVLGAMRGVTRLPLISQANAGLPK
LVDGKTVFPGTPDDMTAFHDRMLGLNVRVIGGCCGTTPTHIRAIKEALAGRDQSWRDCGPPAGVTFLASRTSVVALGGGL
PAAIIGERINPTGKKAFAQELREGKITTIRREAMEQTAAGAHLLDVNVGTPGIDEPAAMERAVFCVTGSVAVPLVLDSSD
PAALERGLKAADGKVLVNSVNGETKSIERVLPLVKKYGAAVIGLALDETGIPETAEGRLAVAERIVAAAEGRGISRNDVV
IDCLTLTVSAEQKRAMETLRAVRLVKEIIGCPTVLGVSNISFGLPRRPLISSAFFAMALAAGLDAAIVNPKEEEMMAAWR
SAMVLLNRDPSAAAYIAAYAGTAAAAPEPAASGEALDIRERLKRAVITGDRENIVALVEEALGQGLEPLQVSNEGLLPGL
EEVGRRFEKNLVFLPQVMQSADTMQAAFGRLREVMKGSPAASRGKILMATVEGDIHDIGKNIVCTLLENHGFEVIDLGKN
VPADRIVGKARELGVDAVGLSALMTTTMTEMENVIARLKEAGIRTFTMVGGAVVTQEYADEIGADLYARDAMEAVARIKA
LLEK
>Mature_804_residues
MKTPFLQAIKERVLVLDGAMGTMLQERGLRPGQSPEELNLTMPDVVAGVHREYLEAGADIIVTNTFGGTRPKLEHYGLEE
KVREINARAVEIAREVCGDRAYVAASMGPTGLFVEPVGEATFDAMAAFFREQAAALIEAGADLITLETFLDIKEIRAAVI
AIREVSPTIPVIAQLTFDNEGRTVLGTPPEAAAVTLAAAGADIVGSNCGLGPDGICAVLGAMRGVTRLPLISQANAGLPK
LVDGKTVFPGTPDDMTAFHDRMLGLNVRVIGGCCGTTPTHIRAIKEALAGRDQSWRDCGPPAGVTFLASRTSVVALGGGL
PAAIIGERINPTGKKAFAQELREGKITTIRREAMEQTAAGAHLLDVNVGTPGIDEPAAMERAVFCVTGSVAVPLVLDSSD
PAALERGLKAADGKVLVNSVNGETKSIERVLPLVKKYGAAVIGLALDETGIPETAEGRLAVAERIVAAAEGRGISRNDVV
IDCLTLTVSAEQKRAMETLRAVRLVKEIIGCPTVLGVSNISFGLPRRPLISSAFFAMALAAGLDAAIVNPKEEEMMAAWR
SAMVLLNRDPSAAAYIAAYAGTAAAAPEPAASGEALDIRERLKRAVITGDRENIVALVEEALGQGLEPLQVSNEGLLPGL
EEVGRRFEKNLVFLPQVMQSADTMQAAFGRLREVMKGSPAASRGKILMATVEGDIHDIGKNIVCTLLENHGFEVIDLGKN
VPADRIVGKARELGVDAVGLSALMTTTMTEMENVIARLKEAGIRTFTMVGGAVVTQEYADEIGADLYARDAMEAVARIKA
LLEK

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=854, Percent_Identity=31.1475409836066, Blast_Score=341, Evalue=2e-93,
Organism=Homo sapiens, GI13162290, Length=298, Percent_Identity=30.8724832214765, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI157266337, Length=301, Percent_Identity=30.8970099667774, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1790450, Length=877, Percent_Identity=31.5849486887115, Blast_Score=340, Evalue=2e-94,
Organism=Escherichia coli, GI1786456, Length=299, Percent_Identity=28.7625418060201, Blast_Score=97, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17535405, Length=849, Percent_Identity=32.6266195524146, Blast_Score=352, Evalue=6e-97,
Organism=Drosophila melanogaster, GI24585077, Length=286, Percent_Identity=25.5244755244755, Blast_Score=70, Evalue=7e-12,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 84748; Mature: 84748

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50970 HCY ; PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTPFLQAIKERVLVLDGAMGTMLQERGLRPGQSPEELNLTMPDVVAGVHREYLEAGADI
CCCHHHHHHHHHHEEECCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCE
IVTNTFGGTRPKLEHYGLEEKVREINARAVEIAREVCGDRAYVAASMGPTGLFVEPVGEA
EEEECCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCHH
TFDAMAAFFREQAAALIEAGADLITLETFLDIKEIRAAVIAIREVSPTIPVIAQLTFDNE
HHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
GRTVLGTPPEAAAVTLAAAGADIVGSNCGLGPDGICAVLGAMRGVTRLPLISQANAGLPK
CCEEECCCCCHHEEEEEECCCHHCCCCCCCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCC
LVDGKTVFPGTPDDMTAFHDRMLGLNVRVIGGCCGTTPTHIRAIKEALAGRDQSWRDCGP
CCCCCEECCCCCHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCC
PAGVTFLASRTSVVALGGGLPAAIIGERINPTGKKAFAQELREGKITTIRREAMEQTAAG
CCCEEEEECCCEEEEECCCCCHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCC
AHLLDVNVGTPGIDEPAAMERAVFCVTGSVAVPLVLDSSDPAALERGLKAADGKVLVNSV
CEEEEEECCCCCCCCCHHHHHEEEEEECCEEEEEEECCCCHHHHHHCCCCCCCEEEEEEC
NGETKSIERVLPLVKKYGAAVIGLALDETGIPETAEGRLAVAERIVAAAEGRGISRNDVV
CCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEE
IDCLTLTVSAEQKRAMETLRAVRLVKEIIGCPTVLGVSNISFGLPRRPLISSAFFAMALA
EEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHH
AGLDAAIVNPKEEEMMAAWRSAMVLLNRDPSAAAYIAAYAGTAAAAPEPAASGEALDIRE
HCCCCEECCCCHHHHHHHHHHHEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
RLKRAVITGDRENIVALVEEALGQGLEPLQVSNEGLLPGLEEVGRRFEKNLVFLPQVMQS
HHHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHH
ADTMQAAFGRLREVMKGSPAASRGKILMATVEGDIHDIGKNIVCTLLENHGFEVIDLGKN
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHCHHHHHHHHHCCCCEEEECCCC
VPADRIVGKARELGVDAVGLSALMTTTMTEMENVIARLKEAGIRTFTMVGGAVVTQEYAD
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHH
EIGADLYARDAMEAVARIKALLEK
HHCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTPFLQAIKERVLVLDGAMGTMLQERGLRPGQSPEELNLTMPDVVAGVHREYLEAGADI
CCCHHHHHHHHHHEEECCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCE
IVTNTFGGTRPKLEHYGLEEKVREINARAVEIAREVCGDRAYVAASMGPTGLFVEPVGEA
EEEECCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCHH
TFDAMAAFFREQAAALIEAGADLITLETFLDIKEIRAAVIAIREVSPTIPVIAQLTFDNE
HHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
GRTVLGTPPEAAAVTLAAAGADIVGSNCGLGPDGICAVLGAMRGVTRLPLISQANAGLPK
CCEEECCCCCHHEEEEEECCCHHCCCCCCCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCC
LVDGKTVFPGTPDDMTAFHDRMLGLNVRVIGGCCGTTPTHIRAIKEALAGRDQSWRDCGP
CCCCCEECCCCCHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCC
PAGVTFLASRTSVVALGGGLPAAIIGERINPTGKKAFAQELREGKITTIRREAMEQTAAG
CCCEEEEECCCEEEEECCCCCHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCC
AHLLDVNVGTPGIDEPAAMERAVFCVTGSVAVPLVLDSSDPAALERGLKAADGKVLVNSV
CEEEEEECCCCCCCCCHHHHHEEEEEECCEEEEEEECCCCHHHHHHCCCCCCCEEEEEEC
NGETKSIERVLPLVKKYGAAVIGLALDETGIPETAEGRLAVAERIVAAAEGRGISRNDVV
CCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEE
IDCLTLTVSAEQKRAMETLRAVRLVKEIIGCPTVLGVSNISFGLPRRPLISSAFFAMALA
EEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHH
AGLDAAIVNPKEEEMMAAWRSAMVLLNRDPSAAAYIAAYAGTAAAAPEPAASGEALDIRE
HCCCCEECCCCHHHHHHHHHHHEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
RLKRAVITGDRENIVALVEEALGQGLEPLQVSNEGLLPGLEEVGRRFEKNLVFLPQVMQS
HHHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHH
ADTMQAAFGRLREVMKGSPAASRGKILMATVEGDIHDIGKNIVCTLLENHGFEVIDLGKN
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHCHHHHHHHHHCCCCEEEECCCC
VPADRIVGKARELGVDAVGLSALMTTTMTEMENVIARLKEAGIRTFTMVGGAVVTQEYAD
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHH
EIGADLYARDAMEAVARIKALLEK
HHCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]