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The map label for this gene is oprF [H]

Identifier: 78221730

GI number: 78221730

Start: 550228

End: 550884

Strand: Reverse

Name: oprF [H]

Synonym: Gmet_0509

Alternate gene names: 78221730

Gene position: 550884-550228 (Counterclockwise)

Preceding gene: 78221731

Following gene: 78221728

Centisome position: 13.78

GC content: 65.75

Gene sequence:

>657_bases
ATGAAACGGACAGCACTCTGCGCTCTCACCATCGCCATGGCAGCCCTAACCGGTTGCGCTCAACCCATGACCCGGACCCA
GACGGGGGCCGCCTTAGGGACTGGTGTCGGCGCGGCGGTCGGCGCGGGGCTCGGCCAGGCAATCGGACGCAACACGGAAT
CAACCCTCATCGGCGCGGCGGCGGGCGCCTTGGCCGGCGGCCTCACCGGCGGAGCCATCGGCAACTACATGGACAAGCAG
GAACAAGCCATGCGGCAGGCCGTTGCCAATGTAGAGGGAGCAAGCATCCAGCGTGACCAGAACGTCCTTGCCGTCACCTT
CAAATCGGACATCCTCTTCCCCATCAATTCGGCCGTCCTCCAGCCGGGAGCAAACGATGAAATCACCCGGGTGGCCCAGG
TCCTCACCCAGTATCCCGCAACGACCATCACCATCGCCGGCCACACCGACAGCACGGGAAGCGACGCCCTCAACCAGACG
CTCTCGGAGCGCCGGGCCACTGCCGTCAAGAACGCCCTGGCAGCCCGCGGCGTAGCCCCGGCCCGCATGAACGCCATCGG
TTTCGGCAAGAGCAAGCCCATTGCCGACAACAGCACCGAGGCAGGGCGCAGCATGAACCGCCGGGTAGAGATCACCATCG
TCCCCCAGCAGCAGTAA

Upstream 100 bases:

>100_bases
GTCCATACAGACCCGTCCCCTTCGTGGTTGTCTCCCCCGGCTGATATGCTATACTGCCTCGTTAATGAGCAACAGTATCT
TTATCCACCAGGGAGGAATT

Downstream 100 bases:

>100_bases
TCGGCTGCACACACGGGATACCTGCGGCCCGCCGGCAACGACGGGCCGTTTTTTCATCCCTTCCGCAGCAGGGCCTCCGC
GAAGGGGAGATCCTCCTCGG

Product: surface antigen protein

Products: NA

Alternate protein names: Root adhesin [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MKRTALCALTIAMAALTGCAQPMTRTQTGAALGTGVGAAVGAGLGQAIGRNTESTLIGAAAGALAGGLTGGAIGNYMDKQ
EQAMRQAVANVEGASIQRDQNVLAVTFKSDILFPINSAVLQPGANDEITRVAQVLTQYPATTITIAGHTDSTGSDALNQT
LSERRATAVKNALAARGVAPARMNAIGFGKSKPIADNSTEAGRSMNRRVEITIVPQQQ

Sequences:

>Translated_218_residues
MKRTALCALTIAMAALTGCAQPMTRTQTGAALGTGVGAAVGAGLGQAIGRNTESTLIGAAAGALAGGLTGGAIGNYMDKQ
EQAMRQAVANVEGASIQRDQNVLAVTFKSDILFPINSAVLQPGANDEITRVAQVLTQYPATTITIAGHTDSTGSDALNQT
LSERRATAVKNALAARGVAPARMNAIGFGKSKPIADNSTEAGRSMNRRVEITIVPQQQ
>Mature_218_residues
MKRTALCALTIAMAALTGCAQPMTRTQTGAALGTGVGAAVGAGLGQAIGRNTESTLIGAAAGALAGGLTGGAIGNYMDKQ
EQAMRQAVANVEGASIQRDQNVLAVTFKSDILFPINSAVLQPGANDEITRVAQVLTQYPATTITIAGHTDSTGSDALNQT
LSERRATAVKNALAARGVAPARMNAIGFGKSKPIADNSTEAGRSMNRRVEITIVPQQQ

Specific function: Known to stabilize the outer membrane [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=218, Percent_Identity=39.9082568807339, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787191, Length=120, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788957, Length=104, Percent_Identity=39.4230769230769, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1786962, Length=77, Percent_Identity=38.961038961039, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 22221; Mature: 22221

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01068 OMPA_1 ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTALCALTIAMAALTGCAQPMTRTQTGAALGTGVGAAVGAGLGQAIGRNTESTLIGAA
CCCHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHH
AGALAGGLTGGAIGNYMDKQEQAMRQAVANVEGASIQRDQNVLAVTFKSDILFPINSAVL
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCEEEEEECCCEEEECHHHHC
QPGANDEITRVAQVLTQYPATTITIAGHTDSTGSDALNQTLSERRATAVKNALAARGVAP
CCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
ARMNAIGFGKSKPIADNSTEAGRSMNRRVEITIVPQQQ
HHHHHCCCCCCCCCCCCCCHHHHCCCCEEEEEEECCCC
>Mature Secondary Structure
MKRTALCALTIAMAALTGCAQPMTRTQTGAALGTGVGAAVGAGLGQAIGRNTESTLIGAA
CCCHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHH
AGALAGGLTGGAIGNYMDKQEQAMRQAVANVEGASIQRDQNVLAVTFKSDILFPINSAVL
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCEEEEEECCCEEEECHHHHC
QPGANDEITRVAQVLTQYPATTITIAGHTDSTGSDALNQTLSERRATAVKNALAARGVAP
CCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
ARMNAIGFGKSKPIADNSTEAGRSMNRRVEITIVPQQQ
HHHHHCCCCCCCCCCCCCCHHHHCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1538702 [H]