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The map label for this gene is cobD [H]

Identifier: 78221708

GI number: 78221708

Start: 532747

End: 533946

Strand: Direct

Name: cobD [H]

Synonym: Gmet_0487

Alternate gene names: 78221708

Gene position: 532747-533946 (Clockwise)

Preceding gene: 78221707

Following gene: 78221712

Centisome position: 13.33

GC content: 64.25

Gene sequence:

>1200_bases
ATGAAAGAAACCTTCGACCACGGCGGCACGGTCTTCGCCGTTGCCCGCCAACTGGGCGTTTCCCCCGATGAGATTCTCGA
CTTCTCGGCGAGCATCAACCCCCTCGGCCCCCCGGTGGAGGCACGCGGAGCGGTGGCGGCCGCCTTTGACCGGCTCGTCC
ACTACCCCGACAGCGGGGCCACGGAACTGCGGGATGCCCTAGCCCGCCGCCACCATCTTCTTCCCGAAAATATCTGCGTT
GCCAATGGCTCCACGGAACTGATTTATCTGGTTCCCCGGCTCGTCGGCGGCAGACGCGGCCTCATCGTCGCCCCCCCCTT
CTCCGAGTATGCCAAGGGGCTCTCCCGGGCCGGCTGGGAGATCGACTATCTGGACCTCTCCCCCGCCGATTGCTTTACCC
TTCACCTGGAGGCTCTCGACCAGCGCCTGGCGAAGGGGTGCGATCTCCTCTTTCTCGCCAATCCCGGCAATCCGACAGGG
GCGCTTATCCCCCGCGAGGTCATCGTTGAGGTCGTCAGCCTCTGCCGCCGCCACGGTACCTTCCTCGTTCTCGATGAGGC
GTTCATCGACTTTTGCGAGGAGGAGTCGGCCACGGACCTGGTGGTCGCAGGGGAAGGGGCTGTGGTACTCCGCTCCATGA
CCAAGTTCTACGCCATTCCGGGGCTCCGACTCGGCTACGCCATGGGGAGCGAGGAGGTAATCGCCCGGCTCTCCGCTCTG
CGGGAGCCCTGGAGCGTCAACACCCTGGCCCAGGCCGCCGGCCTCGCCTGTCTGGCTGACGGGGAGTACCCGGCCCGGAC
CCGCGCCCTCGTGGAGACGGAGCGGGAGTGGCTTTCCGCCGGCATCGCCAGACTGCCGGGGCTTGCGGTCTATCCTTCCG
CCGCCAACTACCTCCTGGTCCGGATTGACGCCGGACCCACGGCCCCGGAGCTGGCCACGCGGCTTCTCGCCGAGCGGGTC
CTCATCCGGGACTGCTCCTCCTTCCGGGGGCTTTCGGAGCGGTTCTTCCGGGTTGCCGTGCGGGGGACAGAGGAGAACAG
AAGGCTTCTGGAATTGTTGGGGAGGGTTTTTGCTGTGGATATCACTCGAAAACACAGAGCAACCGTGTTCTCAGTCAACA
AATTAGTGCAGAAGTGGAGTGAATTTCGAGTGATCCCACTTCTGGTTGTTTTTCACCATGGCATTGAGGATGGTGAGTAG

Upstream 100 bases:

>100_bases
TCCGGAGACCCTGGCTGAAATCTGTTTGCTTGCAGGGTAACACCTTAAAACGGTTTTCCTCTGGGTCCCTGTGCCCCTGT
GGTGAAAGGTTTTACGAATA

Downstream 100 bases:

>100_bases
TTTTCGCATGCAAGCGGTGATCGCGACTTTGTGAAGTTTTCCGGCGCTTGTAAGGCGTTGATAGAAGGCTTTTATAACCG
GGTTAAACCTGGCGGCACAC

Product: L-threonine O-3-phosphate decarboxylase

Products: NA

Alternate protein names: L-threonine-O-3-phosphate decarboxylase [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MKETFDHGGTVFAVARQLGVSPDEILDFSASINPLGPPVEARGAVAAAFDRLVHYPDSGATELRDALARRHHLLPENICV
ANGSTELIYLVPRLVGGRRGLIVAPPFSEYAKGLSRAGWEIDYLDLSPADCFTLHLEALDQRLAKGCDLLFLANPGNPTG
ALIPREVIVEVVSLCRRHGTFLVLDEAFIDFCEEESATDLVVAGEGAVVLRSMTKFYAIPGLRLGYAMGSEEVIARLSAL
REPWSVNTLAQAAGLACLADGEYPARTRALVETEREWLSAGIARLPGLAVYPSAANYLLVRIDAGPTAPELATRLLAERV
LIRDCSSFRGLSERFFRVAVRGTEENRRLLELLGRVFAVDITRKHRATVFSVNKLVQKWSEFRVIPLLVVFHHGIEDGE

Sequences:

>Translated_399_residues
MKETFDHGGTVFAVARQLGVSPDEILDFSASINPLGPPVEARGAVAAAFDRLVHYPDSGATELRDALARRHHLLPENICV
ANGSTELIYLVPRLVGGRRGLIVAPPFSEYAKGLSRAGWEIDYLDLSPADCFTLHLEALDQRLAKGCDLLFLANPGNPTG
ALIPREVIVEVVSLCRRHGTFLVLDEAFIDFCEEESATDLVVAGEGAVVLRSMTKFYAIPGLRLGYAMGSEEVIARLSAL
REPWSVNTLAQAAGLACLADGEYPARTRALVETEREWLSAGIARLPGLAVYPSAANYLLVRIDAGPTAPELATRLLAERV
LIRDCSSFRGLSERFFRVAVRGTEENRRLLELLGRVFAVDITRKHRATVFSVNKLVQKWSEFRVIPLLVVFHHGIEDGE
>Mature_399_residues
MKETFDHGGTVFAVARQLGVSPDEILDFSASINPLGPPVEARGAVAAAFDRLVHYPDSGATELRDALARRHHLLPENICV
ANGSTELIYLVPRLVGGRRGLIVAPPFSEYAKGLSRAGWEIDYLDLSPADCFTLHLEALDQRLAKGCDLLFLANPGNPTG
ALIPREVIVEVVSLCRRHGTFLVLDEAFIDFCEEESATDLVVAGEGAVVLRSMTKFYAIPGLRLGYAMGSEEVIARLSAL
REPWSVNTLAQAAGLACLADGEYPARTRALVETEREWLSAGIARLPGLAVYPSAANYLLVRIDAGPTAPELATRLLAERV
LIRDCSSFRGLSERFFRVAVRGTEENRRLLELLGRVFAVDITRKHRATVFSVNKLVQKWSEFRVIPLLVVFHHGIEDGE

Specific function: Decarboxylates L-threonine-O-3-phosphate to yield (R)-1- amino-2-propanol O-2-phosphate, the precursor for the linkage between the nucleotide loop and the corrin ring in cobalamin [H]

COG id: COG0079

COG function: function code E; Histidinol-phosphate/aromatic aminotransferase and cobyric acid decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788332, Length=308, Percent_Identity=27.5974025974026, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1788627, Length=351, Percent_Identity=25.3561253561254, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1788722, Length=319, Percent_Identity=25.0783699059561, Blast_Score=63, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322075, Length=355, Percent_Identity=27.887323943662, Blast_Score=97, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR005860
- InterPro:   IPR004838
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =4.1.1.81 [H]

Molecular weight: Translated: 43696; Mature: 43696

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00105 AA_TRANSFER_CLASS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKETFDHGGTVFAVARQLGVSPDEILDFSASINPLGPPVEARGAVAAAFDRLVHYPDSGA
CCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCH
TELRDALARRHHLLPENICVANGSTELIYLVPRLVGGRRGLIVAPPFSEYAKGLSRAGWE
HHHHHHHHHHHCCCCCCEEEECCCCEEEEEEHHHHCCCCCEEECCCHHHHHHHHHHCCCE
IDYLDLSPADCFTLHLEALDQRLAKGCDLLFLANPGNPTGALIPREVIVEVVSLCRRHGT
EEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCC
FLVLDEAFIDFCEEESATDLVVAGEGAVVLRSMTKFYAIPGLRLGYAMGSEEVIARLSAL
EEEEHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHH
REPWSVNTLAQAAGLACLADGEYPARTRALVETEREWLSAGIARLPGLAVYPSAANYLLV
CCCCCHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEE
RIDAGPTAPELATRLLAERVLIRDCSSFRGLSERFFRVAVRGTEENRRLLELLGRVFAVD
EECCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEE
ITRKHRATVFSVNKLVQKWSEFRVIPLLVVFHHGIEDGE
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKETFDHGGTVFAVARQLGVSPDEILDFSASINPLGPPVEARGAVAAAFDRLVHYPDSGA
CCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCH
TELRDALARRHHLLPENICVANGSTELIYLVPRLVGGRRGLIVAPPFSEYAKGLSRAGWE
HHHHHHHHHHHCCCCCCEEEECCCCEEEEEEHHHHCCCCCEEECCCHHHHHHHHHHCCCE
IDYLDLSPADCFTLHLEALDQRLAKGCDLLFLANPGNPTGALIPREVIVEVVSLCRRHGT
EEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCC
FLVLDEAFIDFCEEESATDLVVAGEGAVVLRSMTKFYAIPGLRLGYAMGSEEVIARLSAL
EEEEHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHH
REPWSVNTLAQAAGLACLADGEYPARTRALVETEREWLSAGIARLPGLAVYPSAANYLLV
CCCCCHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEE
RIDAGPTAPELATRLLAERVLIRDCSSFRGLSERFFRVAVRGTEENRRLLELLGRVFAVD
EECCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEE
ITRKHRATVFSVNKLVQKWSEFRVIPLLVVFHHGIEDGE
CCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]